Wheat breeding method for improving content of resistant starch of kernels

Abstract

The invention belongs to the technical field of wheat breeding, in particular relates to a wheat breeding method for improving content of resistant starch of kernels and strengthening a health care function. The wheat breeding method for conveniently, rapidly, accurately and efficiently improving content of resistant starch of kernels comprises the steps of: after required variation and breeding generated by variety selection and seed treatment are stabilized, screening materials with reduced main viscosity parameters through starch pasting characteristic measurement, further measuring direct-chain starch content and defining change of physical and chemical properties of starch, and finally confirming materials with increased content of the resistant starch through directly measuring the content of the resistant starch. According to the invention, detection procedures are simplified, selection accuracy and working efficiency are improved, and better effect is obtained in practice.

Description

technical field [0001] The invention belongs to the technical field of wheat breeding, and in particular relates to a wheat breeding method for improving grain resistant starch content and strengthening health care functions. Background technique [0002] At present, the world is facing the serious situation of the rapid spread of chronic non-communicable diseases such as overweight, obesity, and diabetes. The main reason for this phenomenon is that people's diet structure has undergone tremendous changes, and high-calorie foods have increased dramatically. Resistant starch (Resistant starch) is a new type of dietary fiber discovered in recent years. The European Resistant Starch Research Collaborative Network (Euresta) defines it as "the general term for starch and its decomposition products that are not digested and absorbed by the healthy human small intestine." , Resistant starch has a variety of physiological functions (see Table 1). Compared with ordinary dietary fibe...

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Problems solved by technology

Australia's CSIRO has obtained a wheat line containing 70% amylose by silencing the starch branching enzyme gene, hoping to use it to develop resistant starch products, but its yield is very low, which affects its wide-scale application

In addition, genetic modification is feasible, but wheat genetic modification technology still faces many problems, and the safety evaluation is also very cumbersome

[0007] Another important factor affecting the selection of wheat with high resistant starch content is the determination of resistant starch content. The determination of resistant starch is to simulate the digestive process in the human body, which requires the continuous action of various enzymes. The process is complicated, time-consuming and costly. high

At present, there are many methods for the determination of resistant starch, and the results vary widely. Some methods are not easy to operate and have poor reproducibility. For example, Wang Lin et al. found that the content of resistant starch was between 0.87-4.33% in the determination of 100 spring wheats at home and abroad, with an average of 2.11. %; Zhang Pingping found that the content of resistant starch in wheat was 0.64±0.11%

At present, the content of amylose is mostly determined by the iodine-potassium iodide colorimetric method. The reaction conditions such as the pH value of the solution, temperature, and reaction time all strongly affect the color development results, and the operation is relatively cumbersome, resulting in errors.

[0008] Due to the lack of germplasm resources and the complexity of detection methods, the progress of wheat high resistant starch breeding is very slow

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