A method for ultrasensitive detection of dopamine based on nucleic acid aptamer

A nucleic acid aptamer and sensitive detection technology, applied in the field of biomedicine, can solve the problems of large trauma, poor sensitivity, and complicated operation, and achieve the effects of reducing burden, low cost and high sensitivity
CN109975561BActive Publication Date: 2021-11-09HEBEI MEDICAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
HEBEI MEDICAL UNIVERSITY
Publication Date
2021-11-09

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Abstract

The present invention provides a method for ultrasensitive detection of dopamine based on nucleic acid aptamers. First, ssDNA1 is combined with dopamine aptamers and ssDNA2 through the principle of complementary base pairing to form double-stranded DNA1 with two 3' protruding ends, wherein , ssDNA2 has fewer bases than ssDNA1 and is complementary to ssDNA1, and the dopamine aptamer starts from the 3' end of ssDNA2 to partially complement ssDNA1; then add the sample to be tested, hairpin DNA, fluorescent dye SYBR Greenâ… , and exonucleic acid Enzyme III, wherein, the number of bases of the hairpin DNA is less than that of ssDNA1 and can form a double-stranded DNA2 with a recess at the 3' end of the hairpin DNA chain with the free ssDNA1 through the principle of complementary base pairing. In the present invention, the dopamine aptamer recognizes dopamine, so that the aptamer is detached from the double-stranded DNA1, thereby continuously triggering the enzyme cutting reaction, reducing the fluorescence signal, and realizing the supersensitive detection of dopamine by using the cyclic amplification technology.
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Description

technical field

[0001] The invention relates to the field of biomedicine, in particular to a method for ultrasensitive detection of dopamine based on nucleic acid aptamers. Background technique

[0002] Parkinson's disease is a common neurological dysfunction disease that mainly affects middle-aged and elderly people. It is the "third killer" of middle-aged and elderly people after tumors and cardiovascular and cerebrovascular diseases. Surprisingly, of the approximately 4.5 million Parkinson's disease patients worldwide, nearly half are in China. The main pathological change of Parkinson's disease is the degeneration and death of dopaminergic neurons in the substantia nigra of the midbrain, which leads to a significant decrease in the content of dopamine (DA) in the striatum. Therefore, the detection of neurotransmitters such as dopamine is of great significance for the protection of national health and the treatment and prevention of diseases.

[0003] However, due to th...

Claims

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