Preparation method of enzyme-linked immunosorbent assay kit for detecting ovarian cancer tumor marker CA125 based on trypsin fluorogenic substrate

An enzyme-linked immunosorbent reagent, CA125 technology, applied in the direction of measuring devices, biological material analysis, instruments, etc., can solve the problems of lack of obvious symptoms and effective screening, low survival rate, etc., to achieve low detection of non-specific adsorption, high sensitivity, Effect of Improving Detection Sensitivity
CN106442993AInactive Publication Date: 2017-02-22TIANJIN UNIV
1 Cites 5 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
TIANJIN UNIV
Publication Date
2017-02-22
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention relates to a preparation method of an enzyme-linked immunosorbent assay kit for detecting an ovarian cancer tumor marker CA125 based on a trypsin fluorogenic substrate. An enzyme-linked immunosorbent assay technology and a fluorescent detection technology are combined to prepare a detection probe through trypsin and a tumor marker corresponding antibody Ab2; the probe, the tumor marker and another tumor marker antibody Ab1 embedded into the enzyme-linked immunosorbent assay (ELISA) kit can form a structure similar to a sandwich through the action between antigens of the antibodies; finally fluorescence is generated through the action between enzyme and the fluorescent substrate to qualitatively and quantitatively detect the ovarian cancer related tumor marker CA125, and a novel method for detecting protein markers is established. The preparation method has the characteristics that the whole preparation process is simple and suitable for industrial production; the protein markers can be qualitatively and quantitatively detected according to the fluorescent characteristics of the fluorogenic substrate and the action of the fluorogenic substrate with the enzyme, and the specificity is good; the whole detection process is low in cost and very convenient to operate; the novel method for detecting the protein markers is established.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to the technical field of disease detection, and more specifically relates to a method for preparing an enzyme-linked immunoassay kit for detecting ovarian cancer tumor marker CA125 based on a trypsin fluorescent substrate. Background technique

[0002] In recent years, with the rapid changes in people's lifestyles and increasing mental pressure, the incidence of tumors in our country has been increasing year by year. Among them, the mortality rate of ovarian cancer ranks first among gynecological tumors. Unlike breast cancer, ovarian cancer lacks obvious symptoms and effective screening in the early stage, and the survival rate is low. Tumor markers of ovarian cancer can be divided into various subtypes according to their histopathological types, among which epithelial-derived tumors are the most common type, and most of the existing tumor markers are also closely related to ovarian epithelial carcinoma, such as CA125 and human e...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More