2results about How to "Enables ultra-sensitive detection" patented technology

The application discloses a preparation method of a dual-mode CD122 gene thalassemia detection self-powered biosensor, successfully applies an enzyme biological fuel cell (EBFC) to dual-mode detection of CD122 gene thalassemia, realizes quantitative analysis through changes of EBFC output voltage and changes of electrolyte color (RGB Blue value), and mainly concentrates on a cathode of the EBFC in the self-powered design. When CD122 exists, an electrode starts catalytic hairpin self-assembly (CHA) amplification, realizes recycling of CD122, and provides a binding site for subsequent hybridization chain reaction (HCR) amplification, at this time, methylene blue (MB) adsorbed by a biological cathode increases, and an electric signal is enhanced, and meanwhile, electrolyte color becomes lighter (RGB Blue value becomes larger). The self-powered CD122 dual-mode biosensor based on the EBFC disclosed by the application can realize simple, rapid, sensitive and efficient detection of a target.

This invention discloses a controllable synthesis method for programmable perforated sheet-like MOF materials, aiming to solve the problems of cumbersome modification processes and low mass transfer efficiency and activity utilization in traditional MOF material modification. This method employs a one-pot in-situ synthesis strategy, abandoning complex etching processes. By precisely controlling reaction parameters such as the amount of sodium dodecyl sulfate (SDS), the controllable construction of MOF materials from monopores to porous structures is achieved, shortening the preparation cycle to 1.5 hours. By loading hemin chloride into the material and combining it with the "structure-activity positive structure-activity theory," the catalytic activity of the porous structure is verified to be significantly superior to that of the monopore structure. This material has a simple process, controllable cost, and can be used as a highly active carrier for biological probe loading, with wide applications in biosensing, immunoassay, and other fields, especially suitable for POCT detection scenarios, showing broad application potential.