The invention discloses a dual-signal-amplification fluorescence sensor for detecting mercury ions and a preparation method thereof. The fluorescence sensor comprises seven probes, namely a, b, c andd, namely a probe S, a probe F, a probe F1, a probe F2, a probe IP, a probe H1 and a probe H2; fuel stimulation isothermal amplification (FSP) primary circulation is realized based on specific recognition (T- Hg<2+> -T specific base mismatch) of the mercury ions and nucleic acid, an F2 chain released by the FSP can achieve chain displacement catalytic card assembly reaction (CHA) with H1 and H2, secondary circulation is achieved, and lastly the mercury ions are detected through fluorescence signal enhancement. The fluorescence sensor is advantaged in that the sensor has low detection limit, high sensitivity, high reaction speed, strong anti-interference capability, mild reaction conditions and the like, can make up for defects of a mercury ion detection method in the prior art, and realizes rapid and accurate quantitative detection of the mercury ions.