5results about How to "Enables ultra-sensitive detection" patented technology

The invention provides a method for detecting biomolecules based on micro-cantilever sensing, and belongs to the technical field of analysis and detection. The method comprises the following steps: providing a micro-fluidic module which comprises a micro-fluidic channel and a temperature controller, arranging a micro-cantilever array in the micro-fluidic channel, uniformly mixing a to-be-detected sample and a magnetic nanoparticle solution through the micro-fluidic channel, and combining the to-be-detected sample and the magnetic nanoparticle solution on the micro-beam surface of the micro-cantilever array; the laser module provides laser and is used for receiving a micro-beam deflection signal of the micro-cantilever beam array; and providing a magnetic field device, and attracting the magnetic nanoparticles by utilizing magnetic force, so that the micro-cantilever deflection deflection of the micro-cantilever array is increased, a deflection signal is enhanced, and the detection of the to-be-detected sample is realized. The micro-beam is driven by magnetic force to generate deformation, and a relatively large micro-beam deformation signal can be generated under extremely low concentration, so that ultra-sensitive detection of trace substances in a micro sample is realized.

The application discloses a preparation method of a dual-mode CD122 gene thalassemia detection self-powered biosensor, successfully applies an enzyme biological fuel cell (EBFC) to dual-mode detection of CD122 gene thalassemia, realizes quantitative analysis through changes of EBFC output voltage and changes of electrolyte color (RGB Blue value), and mainly concentrates on a cathode of the EBFC in the self-powered design. When CD122 exists, an electrode starts catalytic hairpin self-assembly (CHA) amplification, realizes recycling of CD122, and provides a binding site for subsequent hybridization chain reaction (HCR) amplification, at this time, methylene blue (MB) adsorbed by a biological cathode increases, and an electric signal is enhanced, and meanwhile, electrolyte color becomes lighter (RGB Blue value becomes larger). The self-powered CD122 dual-mode biosensor based on the EBFC disclosed by the application can realize simple, rapid, sensitive and efficient detection of a target.

This invention relates to a microfluidic biosensing platform based on upconversion luminescence, comprising: an upconversion luminescence biosensor for specifically identifying EDCs; and a microfluidic chip for integrating the mixing, reaction, separation, and detection of the upconversion luminescence biosensor and a sample to be tested; the microfluidic chip comprising: a sample inlet for introducing the upconversion luminescence biosensor and the sample to be tested; an arc-shaped channel, the inlet of which is simultaneously connected to the sample inlet of the upconversion luminescence biosensor and the sample inlet of the sample to be tested, wherein the arc-shaped channel is used for mixing and reaction after the upconversion luminescence biosensor and the sample to be tested enter the arc-shaped channel; a separation channel connected to the outlet of the arc-shaped channel for magnetic separation of the upconversion luminescence biosensor after the reaction is completed; and a detection cell connected to the outlet of the separation channel for completing luminescence-enhanced quantitative detection of EDCs.

This invention discloses a controllable synthesis method for programmable perforated sheet-like MOF materials, aiming to solve the problems of cumbersome modification processes and low mass transfer efficiency and activity utilization in traditional MOF material modification. This method employs a one-pot in-situ synthesis strategy, abandoning complex etching processes. By precisely controlling reaction parameters such as the amount of sodium dodecyl sulfate (SDS), the controllable construction of MOF materials from monopores to porous structures is achieved, shortening the preparation cycle to 1.5 hours. By loading hemin chloride into the material and combining it with the "structure-activity positive structure-activity theory," the catalytic activity of the porous structure is verified to be significantly superior to that of the monopore structure. This material has a simple process, controllable cost, and can be used as a highly active carrier for biological probe loading, with wide applications in biosensing, immunoassay, and other fields, especially suitable for POCT detection scenarios, showing broad application potential.

This application discloses a photocatalytic enzyme cascade catalytic material, its preparation method, and its applications. The material comprises a photocatalytic component and a peroxidase-mimicking enzyme component, which are covalently linked. The photocatalytic component generates hydrogen peroxide (H₂O₂) in situ under light irradiation, while the peroxidase-mimicking enzyme component catalyzes the oxidation of the substrate to produce precipitation. This application eliminates the need for exogenous H₂O₂ addition, avoiding high background noise and false positive risks. Simultaneously, the use of carbon dot nanozymes with peroxidase-like activity to replace natural enzymes improves the system's stability and lifespan. By integrating photocatalysis-enzyme cascade amplification and catalytic precipitation technology, this system can transform weak biorecognition events into significant photocurrent signal suppression, achieving ultrasensitive detection of trace biomarkers such as microRNAs and proteins. This application provides a novel strategy for the detection of low-concentration biomolecules.