10 results about "Microarray cgh" patented technology

Use of a compound, microarray chip substrate, microarray chip and use and method thereof. The present application provides use of a compound, or a geometric isomer, tautomer, isotopically labeled, hydrate, solvate or salt thereof in chip surface modification. Also provided is a microarray chip substrate modified by the compound, and a microarray chip comprising the microarray chip substrate. The microarray chip substrate of the present application can greatly improve the surface modification function by surface modification, improve nucleic acid synthesis yield, amplify detection signal, and avoid non-specificity and cross-talk. It has a wide application prospect.

The application discloses a surface enhanced Raman scattering (SERS) analysis platform for detecting early gastric cancer protein biomarkers and a preparation method and application thereof, and belongs to the technical field of detection. The preparation method comprises the following steps: (1) modifying the surface of Au@Pt nano-enzyme (Au@Pt NPs) nano-enzyme with a hairpin structure DNA1 (hpDNA1) to form two SERS probes; (2) modifying hpDNA2 on the surface of a special-shaped nano-pillar array (Au / SiNPA) as a capture substrate; and (3) assembling a 4*4 microarray chip as a SERS analysis platform. The SERS analysis platform has the advantages of simple operation, high throughput, low price, high sensitivity and high specificity, and has good repeatability and can be prepared on a large scale.

The invention relates to the technical field of detection, and relates to a single-molecule protein detection method based on a microarray chip and a chemiluminescence immunoassay technology. An antigen-antibody complex reaction solution is randomly segmented into tens of thousands of microarray holes with the single-hole volume at the pL level by utilizing a microarray chip containing 0.5-200 thousands of micropores with the diameter of 5-30 [mu] m. Each micropore is used as an independent reaction unit for detection and comprises one or zero antigen-antibody compounds, a chromogenic substrate or exciting light is added to collect signal data in all the micropores, and the number of all the antigen-antibody compounds in a reaction system is directly and quantitatively detected according to a Poisson distribution calculation formula. And calculating the concentration of the antigen captured in the microarray chip. The method disclosed by the invention has the advantages of small background signal interference, high sensitivity, specificity, accuracy and precision, simplicity and rapidness in operation, low cost and obvious advantages in the technical field of single molecule detection, and the lower limit of detection reaches fg / ml.

The use of a compound, a microarray chip substrate, and a microarray chip and the use thereof and a method therefor. The present application provides a compound, or a geometric isomer, a tautomer, an isotope-labeled compound, a hydrate, a solvate or a salt thereof in the surface modification of a chip. Further provided is a microarray chip substrate modified by the compound, and a microarray chip comprising the microarray chip substrate.

The embodiment of the invention discloses a manufacturing method of a pathological tissue microarray chip of a 3D cell model, a culture chip for preparing the 3D cell model is adopted for manufacturing, the culture chip is dried after being subjected to high-temperature and high-pressure sterilization, and low-adhesion treatment is performed for standby application; selecting a cell sample with a good growth state, and culturing a 3D cell model by utilizing the treated culture chip and the cell sample; directly performing embedding pretreatment on the 3D cell model in the culture chip to obtain a pre-embedded block comprising the 3D cell model; carrying out sample embedding treatment on the pre-embedded block by adopting OCT (Optical Coherence Tomography) glue to obtain a sample embedded block; and carrying out frozen section operation on the sample embedding block by adopting a freezing slicer to obtain the pathological tissue microarray chip. The steps of sample collection, transfer, centrifugation and the like can be omitted, the problems of sample loss, damage and the like are effectively avoided, the slicing operation is simplified, and the slicing efficiency is improved; besides, by utilizing array positioning of the culture micropores, the conditions of sample aggregation, deformation, extrusion damage, overlapping shielding and the like are avoided, and the requirements of visualization, data acquisition and statistics, spatial positioning and the like of sample monomers are met.

The invention relates to a dual-channel microarray chip, a signal probe and a detection method for detecting exosome RNA (Ribonucleic Acid), which are characterized in that specific replication is carried out on target RNA through LCR (Like, Chromatography and Regulation) amplification reaction so as to fulfill the aim of amplifying the number of target objects and amplifying signals; then combining a fragment obtained by amplification with gold-coated magnetic nanoparticles to construct an electrochemical signal probe; purifying the signal probe through a magnetic separation technology, and re-dispersing the signal probe in a PBS solution; and fixing the signal probe on the microarray chip. According to the present invention, with the cooperation of the dual-channel switching assembly, the electrochemical detection system and the matched software control platform, the synchronous detection and analysis of the dual-target exosome RNA can be achieved, and the high detection sensitivity and the high specificity are provided so as to significantly improve the accuracy and the result reliability in the early screening and diagnosis.

The invention relates to a single molecule rapid detection method and system. The single molecule rapid detection method comprises the following steps: placing a to-be-detected microarray chip subjected to an in-situ reaction on a detection table of the detection system; the detection system turns on a bright field light source; the fluorescence camera starts an automatic focusing function, and automatic focusing is completed within 1 s; the fluorescence camera locks a focusing position parameter; the detection system turns off the bright field light source and turns on the fluorescence excitation light source; controlling the fluorescence camera to perform exposure, and collecting a fluorescence image; inputting the fluorescence image into a deep learning model, and identifying and outputting position information of all single-molecule fluorescence spots in the fluorescence image; counting the number of light spots falling into each unit on the microarray chip; on the basis of the Poisson distribution principle, the number of single molecule spots obtained through statistics serves as a direct counting result, and the absolute concentration or the absolute copy number is calculated. According to the invention, rapid, high-precision and absolute quantitative single molecule detection is realized.

The application belongs to the technical field of biology, and discloses a microarray chip, a preparation method and application thereof. The microarray chip has hydrophilic reaction holes and hydrophobic non-reaction areas, the depth of the reaction holes is 20-50 mu m, the etching depth is much higher than the depth of 3-10 mu m of traditional laser etching, the hydrophobic angle of the non-reaction areas is more than 110 degrees, and the chip surface is flat and smooth. The application adopts double photolithography and surface hydrophobization treatment, and can quickly prepare the microarray chip with multiple hydrophilic reaction holes and hydrophobic non-reaction areas, which meets the needs of large-scale industrial production; different patterned masks can be designed to obtain reaction holes with different shapes for different detection scenes.

The application discloses a screening method of molecular glue drugs and application in degradation drug screening. The screening method of molecular glue drugs comprises the following steps: preparing a first microarray chip from a compound library, screening compounds combined with an effector protein based on an OI-RD image to obtain first candidates; preparing a second microarray chip, measuring binary binding kinetics of the first candidates and the effector protein based on OI-RD optical real-time signals, verifying, and obtaining second candidates; and preparing a third microarray chip, screening compounds for ternary reactions based on OI-RD real-time signals of ternary reactions. The screening method directly detects the formation of a target protein-molecular glue-effector protein ternary complex, significantly improves screening accuracy, can effectively identify molecular glue types that do not directly bind to target proteins, and realizes higher specificity and higher flux molecular glue screening.

The invention discloses an FELDI nano-particle chip kit and application thereof in screening metabolic marker combinations, and relates to the technical field of biology. The FELDI nano-particle chip kit comprises a methanol solution and a microarray chip which is pre-coated with iron nano-particles, the kit is used for dewaxing tissue slices, the tissue slices are immersed in a methanol solution and incubated to obtain a sample, and the sample is applied to a microarray chip pre-coated with iron nanoparticles; a colorectal cancer tissue metabolism fingerprint database is constructed, and specific metabolism markers combined with L-cysteine, adipic acid, decanoic acid, azelaic acid, pentadecanoic acid, stearic acid and isolithocholic acid are screened. The invention develops an unmarked iron nanoparticle enhanced laser desorption ionization mass spectrometry technology platform, and the small molecule metabolites can be directly and efficiently captured and ionized from the formalin-fixed paraffin-embedded CRC primary tissue section. And screening out a specific small molecule metabolism marker combination for distinguishing lymphatic metastasis and non-metastasis.