47 results about "Microarray cgh" patented technology

The invention belongs to the field of biomedical detection, and particularly relates to a protein chip, a detection kit based on a polypeptide coding microarray chip and application of the detection kit. According to the protein chip provided by the invention, an antibody and a bispecific antibody of corresponding indexes can be added into a treating fluid according to detection requirements, the antibody and the bispecific antibody are combined with the chip in a manner of polypeptide specific recognition after being subjected to antigen specific recognition respectively, and joint detection of different antigen combinations and detection of a single index are carried out. By utilizing the polypeptide sample application chip and the antigen and antibody combined bispecific antibody provided by the invention, the universality of the chip in use, the high efficiency of detection and the low cost can be remarkably improved.

The invention relates to a detection method, in particular to a method for rapidly detecting alpha and beta-lactoglobulin in auxiliary material lactose based on a microarray chip technology. On the basis of an indirect competitive enzyme-linked immunosorbent assay principle and a silver enhanced developing technology, a high-sensitivity microarray chip detection platform is successfully constructed by virtue of a biological chip scanner, and rapid quantitative analysis of alpha-lactalbumin and beta-lactoglobulin in the auxiliary material lactose for injection is realized. According to the method, an antigen-antibody specific recognition mechanism and a nano-silver signal amplification mechanism are integrated, and the detection efficiency and sensitivity are remarkably improved through the high-throughput layout of the microarray chip. In the aspect of methodology performance, the technology has the remarkable advantages of simple and convenient operation process, short detection time, high sample treatment flux, excellent specificity (CRlt; 1%) and the like.

The invention belongs to the field of neuroblastoma tissue analysis, and particularly discloses a child neuroblastoma tissue microarray chip and a preparation method thereof.The child neuroblastoma tissue microarray chip comprises a glass slide base material, and a plurality of neuroblastoma tissue samples arranged in a dot-matrix mode are arranged on the glass slide base material; by integrating a plurality of neuroblastoma samples including various pathological typing, grading and clinical staging types of neuroblastoma in a tissue block, efficient sample processing and analysis are achieved, comprehensiveness and representativeness of research are ensured, a large number of samples are analyzed simultaneously through a microarray technology, and the accuracy and accuracy of analysis are improved. The requirements for high-throughput screening of specific genes and expression proteins thereof in scientific research activities are met, the prediction capacity for tumor prognosis and the guidance accuracy for treatment schemes are improved, and neuroblastoma tissues are analyzed more efficiently and comprehensively.

The invention discloses a surface enhanced Raman scattering (SERS) analysis platform for detecting early gastric cancer protein biomarkers as well as a preparation method and application thereof, and belongs to the technical field of detection. The preparation method comprises the following steps: (1) modifying the surface of Au (at) Pt nano particle (Au (at) PtNPs) nano enzyme with DNA1 (hpDNA1) of a hairpin structure to form two SERS probes; (2) modifying hpDNA2 on the surface of the special-shaped nanorod array (Au / SiNPA) to serve as a capture substrate; (3) assembling a 4 * 4 microarray chip as an SERS (Surface Enhanced Raman Scattering) analysis platform; the SERS analysis platform has the advantages of simplicity in operation, high throughput, low price, high sensitivity and specificity and the like; moreover, the repeatability is good, and large-scale preparation can be realized.

The application discloses a kind of based on digital droplet microfluidic multi-sample flux multi-index detection device and application thereof, belong to electromechanical device, microfluidic chip and immunoprotein analysis field.The device includes droplet microfluidic equipment and immunoprotein analysis chip component, droplet microfluidic equipment is used to drive multiple droplets, mainly divided into three major systems: power supply system, computer operating system and chip clamp system, immunoprotein analysis chip component includes biological analysis substrate and microarray chip.The present application drives micro liquid sample by digital droplet microfluidic technology, realizes the multi-index detection of automated immunoprotein analysis chip.The detection device of the present application has a series of advantages such as rapidity, independence, low consumption, automation, function integration and multi-index detection, and is expected to become the most fruitful rapid diagnostic device for improving tumor diagnosis positive rate.

The utility model discloses a draw-pull type shock absorption microarray chip detection kit which comprises a kit body, the kit body comprises a box body and a box cover covering the box body, a cooling box and a plurality of parallel microarray chip placing layers are sequentially arranged in the box body from bottom to top, an opening is formed in the front side face of the box body, and the microarray chip placing layers are arranged in the box body. The plurality of microarray chip placement layers and the cooling box can be pulled out from the opening in the front side surface; the adjacent microarray chip placing layers are connected through sponges, and the microarray chip placing layers and the cooling box are connected through sponges. The upper portion of the cooling box is open, an ice bag is arranged in the cooling box, cold air emitted by the ice bag is conducted upwards from the bottom through a hollow area in the sponge, and the refrigeration and heat preservation effects on the microarray chip are achieved. The utility model solves the technical problems that the kit is easy to break due to vibration in the transportation process, the insertion is inconvenient, and the temperature is not easy to control.

The invention discloses a DNA microarray chip with an ordered nanostructure and application thereof, the DNA microarray chip is mainly composed of an ordered silicon film array formed by self-assembly of SiO2 microspheres, the particle size of the silicon spheres is 250-650nm, a single-layer densely arranged ordered nanostructure is formed on the surface of a silicon wafer, and probes are distributed on the silicon wafer modified by an aldehyde group to form a spot array. The silicon film array is used as a substrate, SiO2 microspheres are self-assembled on a silicon wafer, the SiO2 microsphere array has optical characteristics, movement of DNA to a solid-liquid interface is accelerated, the probability of collision between DNA and probe DNA is increased, and finally DNA hybridization efficiency and imaging efficiency are improved.

Methods of detecting a leak from a subarray of a microarray chip, kits of components that facilitate leak detection, and microarray chips configured for leak detection are disclosed herein. The methods include positioning, within the given subarray, a sample solution, which includes dissolved sample molecules (DSMs) that define a dissolved sample oligonucleotide sequence and dissolved leak detection molecules (DLDMs) that define a predetermined dissolved leak detection oligonucleotide sequence. The methods also include detecting the DLDMs within a region of the microarray chip that is external the given subarray. The kits of components include the microarray chip and DLDMs. The microarray chips includes a plurality of subarrays and a leak detection region that is at least partially external the plurality of subarrays.

Use of a compound, microarray chip substrate, microarray chip and use and method thereof. The present application provides use of a compound, or a geometric isomer, tautomer, isotopically labeled, hydrate, solvate or salt thereof in chip surface modification. Also provided is a microarray chip substrate modified by the compound, and a microarray chip comprising the microarray chip substrate. The microarray chip substrate of the present application can greatly improve the surface modification function by surface modification, improve nucleic acid synthesis yield, amplify detection signal, and avoid non-specificity and cross-talk. It has a wide application prospect.

The present invention relates to a single nucleotide polymorphism marker for precocious puberty diagnosis or treatment prognosis prediction for the disease, and a use thereof. In the present invention, the DNA of 700 girl patients of precocious puberty is processed on a microarray chip containing a total of 8,754,887 SNP probes so that SNP genotype data is obtained, and 23 SNPs related to the risk of developing precocious puberty are discovered by means of genome-wide association analysis so that the SNPs can be used for precocious puberty diagnosis or treatment prognosis prediction, early diagnosis of precocious puberty can be conveniently and rapidly carried out, and the prediction of treatment effects and prognosis can be carried, and thus the present invention can be effectively used in a personalized treatment.

The invention discloses a molecular glue drug screening method and application in degradable drug screening. A molecular gel drug screening method comprises the following steps: preparing a compound library into a first microarray chip, and screening compounds combined with effect protein based on an OI-RD image to obtain a first candidate; preparing a second microarray chip, measuring binary binding kinetics of the first candidate and the effect protein based on an OI-RD optical real-time signal, and verifying to obtain a second candidate; and preparing a third microarray chip, and screening a compound subjected to the ternary reaction based on an OI-RD real-time signal of the ternary reaction. According to the screening method disclosed by the invention, by directly detecting the formation of the target protein-molecular glue-effect protein ternary complex, the screening accuracy is remarkably improved, the type of the molecular glue which is not directly combined with the target protein can be effectively identified, and molecular glue screening with higher specificity and higher flux is realized.

The application discloses a surface enhanced Raman scattering (SERS) analysis platform for detecting early gastric cancer protein biomarkers and a preparation method and application thereof, and belongs to the technical field of detection. The preparation method comprises the following steps: (1) modifying the surface of Au@Pt nano-enzyme (Au@Pt NPs) nano-enzyme with a hairpin structure DNA1 (hpDNA1) to form two SERS probes; (2) modifying hpDNA2 on the surface of a special-shaped nano-pillar array (Au / SiNPA) as a capture substrate; and (3) assembling a 4*4 microarray chip as a SERS analysis platform. The SERS analysis platform has the advantages of simple operation, high throughput, low price, high sensitivity and high specificity, and has good repeatability and can be prepared on a large scale.

The invention belongs to the technical field of type 2 diabetes mellitus, and particularly relates to a type 2 diabetes mellitus risk detection kit and a detection method, the type 2 diabetes mellitus risk detection kit comprises PCR (polymerase chain reaction) amplification primers of SNP (single nucleotide polymorphism) sites: SEQ IQ NO.1-4, SEQ IQ NO.7-10, SEQ IQ NO.13-16, SEQ IQ NO.19-22, SEQ IQ NO.25-28, SEQ IQ NO.31-34, SEQ IQ NO.37-40, SEQ IQ NO.43-46, SEQ IQ NO.49-52 and SEQ IQ NO.55-58. The detection method comprises the following steps: extracting and purifying a sample to obtain a gDNA sample to be detected; in a PCR amplification system, adding a gDNA sample to be detected for amplification; mixing the amplified product with a hybridization reagent system, and carrying out hybridization incubation; scanning the hybridized microarray chip by adopting a scanner, and collecting and recording a fluorescence signal. Through the design of specific primers and probes, the application of the high-density SNP microarray chip and the construction of a weighted genetic risk model, the high-sensitivity and high-specificity detection of the type 2 diabetes related SNP loci is realized; further, the disease risk of the individual type 2 diabetes mellitus is accurately evaluated, and a powerful basis is provided for early prevention and intervention of diseases.

The invention relates to the technical field of detection, and relates to a single-molecule protein detection method based on a microarray chip and a chemiluminescence immunoassay technology. An antigen-antibody complex reaction solution is randomly segmented into tens of thousands of microarray holes with the single-hole volume at the pL level by utilizing a microarray chip containing 0.5-200 thousands of micropores with the diameter of 5-30 [mu] m. Each micropore is used as an independent reaction unit for detection and comprises one or zero antigen-antibody compounds, a chromogenic substrate or exciting light is added to collect signal data in all the micropores, and the number of all the antigen-antibody compounds in a reaction system is directly and quantitatively detected according to a Poisson distribution calculation formula. And calculating the concentration of the antigen captured in the microarray chip. The method disclosed by the invention has the advantages of small background signal interference, high sensitivity, specificity, accuracy and precision, simplicity and rapidness in operation, low cost and obvious advantages in the technical field of single molecule detection, and the lower limit of detection reaches fg / ml.

The use of a compound, a microarray chip substrate, and a microarray chip and the use thereof and a method therefor. The present application provides a compound, or a geometric isomer, a tautomer, an isotope-labeled compound, a hydrate, a solvate or a salt thereof in the surface modification of a chip. Further provided is a microarray chip substrate modified by the compound, and a microarray chip comprising the microarray chip substrate.

The invention provides a microarray chip image identification method and system, and the method comprises the steps: positioning a Block external rectangular frame of an array through a Block target detection model, and enabling the Block target detection model to be generated through the training of a predetermined target detection model through an image set which is formed by a plurality of arrays; performing semantic segmentation on the Block bounding rectangular frame area by using a Spot semantic segmentation model to generate a spot image block mask image, and adjusting the angle of the Block bounding rectangular frame based on the projection result of the spot image block mask image; and calculating by using the spot image block mask graph to obtain the center-of-mass coordinate and radius of each spot area in the adjusted Block circumscribed rectangular frame, and finally determining the circumscribed circle of each spot image block. According to the method, the position of the Block can be accurately recognized, the Spot can be accurately aligned, meanwhile, the analysis efficiency is greatly improved, and the analysis time is shortened.

The invention discloses a radiation dose evaluation model related to gene expression level and application, the genes are MDM2 and TNFSF4, and a construction method of the model comprises the following steps: 1) acquiring microarray chip data after different types of ray irradiation on a human peripheral blood sample; and 2) taking expression quantities of MDM2 and TNFSF4 genes in the microarray chip data as independent variables, taking the radiation dose as a dependent variable, and establishing a multiple linear regression equation by using SPSS software to obtain a radiation dose evaluation model. The model is good in linearity and high in accuracy, shows specificity and sensitivity, and is used for accurately detecting the radiation dose after the human body is exposed to radiation.

The invention relates to the technical field of tissue microarray chips, in particular to a multi-element integrated multi-organ normal tissue microarray chip and an implementation method thereof. The multi-element integrated multi-organ normal tissue microarray chip mainly comprises a glass slide base, a special label for Zhongsha and a sample application tissue sheet with target information, wherein the sample application tissue sheet is attached to a glass slide; a target information sample application tissue piece is carried on the glass slide base, and the tissue point spacing of the target information sample application tissue piece corresponds to the preset tissue point diameter; wherein the target information sample application tissues on the glass slide base are arranged from left to right according to a preset row and column mode, in the horizontal direction, from left to right, each row is numbered by numbers in sequence, in the vertical direction, each column is numbered according to an alphabetic sequence, and 702-4 points can not be equal on each glass slide base. The technical scheme provided by the invention has the advantages of high throughput, high efficiency, parallelism, large information amount, small error and the like.

The invention discloses an in-situ Morse code fast addressing microarray chip unit and a preparation method thereof, the microarray chip unit comprises a super-wetting microchip array substrate and a super-wetting microchip array which is coded by referring to Morse codes, and the chip unit on a two-dimensional plane is combined with the pattern characteristics of the Morse codes. The whole chip array is divided into a plurality of areas, and the chip units in each area can be marked and positioned by using characters and numbers of Morse codes. And after the chip array substrate is subjected to ultraviolet radiation through the mask plate, forming the patterned array chip of the Morse code. The method does not depend on time-consuming and labor-consuming positioning means such as global scanning imaging and manual counting, meanwhile, the coding mode of Morse coding is combined with existing patterns in an array, the coding positioning position can also serve as a detection part, coding positioning and detection are integrated, scanning of a microscope can be efficiently and accurately guided, and the detection accuracy is improved. And the occupation of the detection area can be minimized.

The embodiment of the invention discloses a manufacturing method of a pathological tissue microarray chip of a 3D cell model, a culture chip for preparing the 3D cell model is adopted for manufacturing, the culture chip is dried after being subjected to high-temperature and high-pressure sterilization, and low-adhesion treatment is performed for standby application; selecting a cell sample with a good growth state, and culturing a 3D cell model by utilizing the treated culture chip and the cell sample; directly performing embedding pretreatment on the 3D cell model in the culture chip to obtain a pre-embedded block comprising the 3D cell model; carrying out sample embedding treatment on the pre-embedded block by adopting OCT (Optical Coherence Tomography) glue to obtain a sample embedded block; and carrying out frozen section operation on the sample embedding block by adopting a freezing slicer to obtain the pathological tissue microarray chip. The steps of sample collection, transfer, centrifugation and the like can be omitted, the problems of sample loss, damage and the like are effectively avoided, the slicing operation is simplified, and the slicing efficiency is improved; besides, by utilizing array positioning of the culture micropores, the conditions of sample aggregation, deformation, extrusion damage, overlapping shielding and the like are avoided, and the requirements of visualization, data acquisition and statistics, spatial positioning and the like of sample monomers are met.

The invention discloses a primer, a probe and a microarray chip for detecting mycobacterium tuberculosis and drug resistance of the mycobacterium tuberculosis, the screened primer and the probe can be used for detecting the drug resistance of the mycobacterium tuberculosis and isoniazide and rifampicin through PCR (Polymerase Chain Reaction) amplification and fluorescence scanning by taking sputum as a sample; the screened probe can be specifically combined with a corresponding target gene. The microarray chip prepared by utilizing the screened probe can be specifically combined with a corresponding target gene, is relatively strong and stable in hybridization signal, can take a sputum sample as a detection object, and does not need invasion or anesthesia. The invention establishes a microarray chip detection method which is good in specificity, high in sensitivity, strong in stability, time-saving and labor-saving.

The invention discloses a human CYP2C19 genotype detection method based on a near infrared spectrum technology, a detection model and a model construction method. The detection method comprises the following steps: collecting a human blood sample for near infrared transmission and reflection spectrum detection; determining the CYP2C19 genotype in the human blood sample based on a microarray chip method; the method comprises the following steps: constructing a data matrix of a CYP2C19 genotype and a near-infrared transmission and reflection spectrum of the CYP2C19 genotype, preprocessing data, and establishing a discriminant analysis model; and inputting near infrared reflection spectrum data of a blood sample to be detected into the discriminant analysis model to obtain the CYP2C19 genotype of the blood sample to be detected. According to the method, the data block model is established on the basis of the near infrared spectrum data, the method has the advantages of being rapid, simple and economical in data acquisition method, rapid and accurate recognition of the CYP2C19 gene can be achieved, and rapid and accurate guidance suggestions can be provided for clinical medication.

Disclosed are methods of determining or predicting whether a subject is having or likely to have a proliferative disease, methods of predicting the likelihood of a subject surviving proliferative disease (prognosis of a subject), a kit or microarray chip, methods for manufacturing a microarray chip or protein array chip, and methods of treating or preventing a proliferative disease.

The invention provides a preparation method, a product and application of an SERS (Surface Enhanced Raman Scattering) sensor for detecting a colorectal cancer precancerous lesion biomarker, and belongs to the technical field of biology. The preparation method comprises the following steps: firstly, obtaining a nanoring array by utilizing a liquid-liquid interface self-assembly method, annealing treatment and gold magnetron sputtering treatment, and then coupling and modifying Raman signal molecules and aptamer complementary chains to the surface of a gold nanocube through Au-S bonds so as to prepare two SERS nanoprobes; an aptamer chain is modified on the surface of a nanoring array, an SERS nanoprobe is combined with a complementary basic group in the aptamer chain and is fixed on the nanoring array, and the SERS sensor is constructed. The SERS sensor is embedded into the PDMS chip to obtain the SERS microarray chip, and the SERS microarray chip has the advantages of high sensitivity, high specificity, high detection speed, high flux and the like.

The utility model discloses a mixing carrier for sequencing a micro-array chip, which belongs to the field of biochips and comprises a bottom plate, hangers symmetrically arranged on two sides of the bottom plate, positioning grooves arranged on the bottom plate and pressing blocks arranged between the hangers and the bottom plate, and the positioning of the micro-array chip for sequencing is realized through the positioning grooves of the bottom plate. Fixing is achieved through the pressing blocks, the device can be fixed to a mixing instrument through the hanging lugs to conduct swing mixing, the structure is simple, operation is convenient, the reliability of micropore mixing can be ensured, the connecting grooves and the connecting protruding blocks are arranged on the periphery of the bottom plate, mixing operation can be conducted on multiple chips in a splicing mode, and the use flexibility is high.

The invention relates to the technical field of biomedicine, and particularly discloses application of an lncRNA marker based on m6A modification in prognosis prediction of colorectal cancer. Constructing a multi-dimensional prognosis evaluation model by integrating the lncRNA expression quantity, the m6A modification level and clinical pathological parameters; according to the technical scheme, the method comprises the steps that m6A modified lncRNA markers related to colorectal cancer prognosis are screened, the expression and modification states of the m6A modified lncRNA markers are quantitatively analyzed through qRT-PCR and m6A antibody detection technologies, a prognosis risk score is calculated in combination with clinical parameters, and accurate layering of the recurrence risk and survival time of a patient is achieved. The matched products comprise a detection kit, a microarray chip and data analysis software, are simple and convenient to operate and are suitable for clinical laboratories and third-party detection. The method breaks through the limitation of a traditional prognosis method, the prediction accuracy is remarkably improved, a scientific basis is provided for personalized diagnosis and treatment, and the method has wide clinical application prospects.

The present invention relates to the field of digital PCR, and specifically to a multi-layer three-dimensional digital nucleic acid amplification chip and a method for making and using the same. In the multi-layer three-dimensional digital nucleic acid amplification chip of the present invention, multi-layer superposition of microarray chips can be achieved, greatly increasing the number of reaction units for digital nucleic acid amplification, so that the dynamic range of nucleic acid quantification and the accuracy of the lower limit of detection are greatly improved, which is conducive to greatly increasing the reaction flux in actual detection. In addition, the present invention also provides a method for making and using the multi-layer three-dimensional digital nucleic acid amplification, which can further improve the accuracy of the quantitative results.

Methods of detecting a leak from a subarray of a microarray chip are disclosed herein. The methods include positioning, within the subarray, a sample solution that includes dissolved sample molecules (DSMs) that define a dissolved sample oligonucleotide sequence and dissolved leak detection molecules (DLDMs) that define a predetermined dissolved leak detection oligonucleotide sequence. The methods also include leaking the sample solution from the subarray to an adjacent subarray and detecting the leak by detecting the DLDMs within the adjacent subarray.

The present application relates to the quality inspection method of microarray chip scale production, comprising: step 1) the nanometer membrane is placed in clean environment, humidity <20%, temperature 23~25 ℃ for a period of time to make the nanometer membrane fully balanced;Step 2) preparation of sample solution, present preparation present use;Step 3) the nanometer membrane of step 1) is fully balanced, is placed in sample point instrument, is gradually sampled in the quality inspection point of nanometer membrane with the sample solution of step 2), each hole quality inspection point is not less than 2, 20nl / point;Step 4) the nanometer membrane of step 3) is sampled is loaded into the matching fixture, is placed under the lens of industrial camera, is photographed with industrial camera, is measured with software for each quality inspection point diameter and is exported;Step 5) analysis of the diameter data of each quality inspection point on the nanometer membrane, the overall standard deviation of the diameter of the quality inspection point on the nanometer membrane, the median and the range, the overall standard deviation ≤5%, the median 460 μm-480 μm, the range ≤30 μm is the nanometer membrane of quality inspection qualified.

The application relates to a microarray chip-based SERS serum analysis method, belonging to the cross field of medicine and artificial intelligence, and a technical scheme is as follows: by using the prepared microarray chip composed of AuNOs arrays, high-throughput spectrum acquisition can be realized, and the signal intensity of extremely weak biological components can be significantly amplified; SERS is combined with the microarray chip to overcome the problems of changes in spectrum peak position and intensity caused by the exposed environment in serum SERS detection; a spectrum recognition analysis model is proposed, which can be used for recognizing serum spectra at different stages, capturing and distinguishing several key spectral characteristics at different stages, effectively improving the accuracy, sensitivity and specificity of recognizing similar SERS spectra, so that the liver injury caused by cisplatin (CILI) can be accurately recognized and analyzed at the molecular level.