The application belongs to the technical field of
biology, and discloses a
microarray chip, a preparation method and application thereof. The
microarray chip has hydrophilic reaction holes and hydrophobic non-reaction areas, the depth of the reaction holes is 20-50 mu m, the
etching depth is much higher than the depth of 3-10 mu m of traditional
laser etching, the hydrophobic angle of the non-reaction areas is more than 110 degrees, and the
chip surface is flat and smooth. The application adopts double
photolithography and surface hydrophobization treatment, and can quickly prepare the
microarray chip with multiple hydrophilic reaction holes and hydrophobic non-reaction areas, which meets the needs of large-scale industrial production; different patterned masks can be designed to obtain reaction holes with different shapes for different detection scenes.