216 results about "Microarray cgh" patented technology

The present invention provides compositions providing and methods using fluorescence detection device, comprising an electroluminescent light (EL) source, for measuring fluorescence in biological samples. In particularly preferred embodiments, the present invention provides an economical, battery powered and Hand-held device for detecting fluorescent light emitted from reporter molecules incorporated into DNA, RNA, proteins or other biological samples, such as a fluorescence emitting biological sample on a microarray chip. Further, a real-time hand-held PCR Analyzer device comprising an EL light source for measuring fluorescence emissions from amplified DNA is provided.

The present invention provides a microarray chip for use in the analysis of various sample types. The microarray chips disclosed herein generally comprise a substrate covered with a coating material comprising a photoresist material, wherein the coating material is patterned to comprise a plurality of microstructures such as microwells and / or microcolumns. Methods for preparing and utilizing the microarray chips of the invention are further provided. The microarray chips of the instant invention find particular use in high-throughput assays.

The present invention relates to a method for the preparation of compound of microarray chips, especially the method by repeatedly impressing reagents at fixed locations on a substrate to synthesize said chips; and also realtes to a compound microarray chips produced according to said method.

Methods and apparatus relate to reduction of sequence errors generated during synthesis of nucleic acids on a microarray chip. The error reduction can include synthesis of complementary stands (to template strands), using a short universal primer complementary to the template strands and polymerase. Heteroduplex can be formed be melting and re-annealing complementary stands and template strands. The heteroduplexes containing a mismatch can be recognized and cleaved by a mismatch endonuclease. The mismatch-containing cleaved heteroduplexes can be removed from the microarray chip using a global buffer exchange. The error free synthetic nucleic acids generated therefrom can be used for a variety of applications, including synthesis of biofuels and value-added pharmaceutical products.

The present invention relates to pathogen detection and identification by use of DNA resequencing microarrays. The present invention also provides resequencing microarray chips for differential diagnosis and serotyping of pathogens present in a biological sample. The present invention further provides methods of detecting the presence and identity of pathogens present in a biological sample.

The invention discloses a microarray chip without solid walls based on LSPR (Localized Surface Plasmon Resonance) and application thereof. The substrate of the microarray chip is in a hydrophilic and hydrophobic mode and comprises a base, a plurality of hydrophilic regions and hydrophobic regions, wherein the hydrophilic regions are arranged on the base; the hydrophobic regions separate the hydrophilic regions; the surfaces of the hydrophilic regions are provided with metal nanometer material layers with local area surface plasma resonance attributes; the outer regions of the hydrophilic regions are not provided with the solid walls; the surfaces of the hydrophobic regions are provided with hydrophobic material layers. The microarray chip disclosed by the invention can be obtained by combining a peculiar affinitive molecule for detecting a substance to be detected on the substrate provided by the invention. The microarray chip disclosed by the invention has the advantages of easiness and convenience for use, low detection cost, accurate result, reusability, and the like, can be used for detecting multiple substances, such as micromolecules, heavy metal ions, proteins, bacteria, viruses, and the like, and has wide application prospect in the fields of medical health, environmental monitoring, scientific experiments, and the like.

The invention relates to an immune gene prognosis model for predicting hepatocellular carcinoma tumor immune infiltration and postoperative survival time, and belongs to the technical field of biological medicines. The model can be used for evaluating the infiltration degree of immune cells in a tumor in clinical practice by detecting the expression levels of 22 specific immune related genes of ahepatocellular carcinoma patient, so that the model can be used for predicting hepatocellular carcinoma tumor immune infiltration in clinical practice and improve the prediction capability of the liver cancer immunotherapy response. The model can be used for judging the postoperative overall survival risk of a patient and guiding the formulation of a postoperative treatment strategy, and the corresponding microarray chip kit can realize the standardization and convenience of detection. Meanwhile, the immune gene prognosis model provided by the invention can increase the prediction accuracy andthe clinical net income of a hepatocellular carcinoma TNM staging system on the total survival time of three years and five years after operation. As a molecular marker for objectively and accuratelyevaluating the tumor immune state and poor prognosis risk of hepatocellular carcinoma, the model can realize accurate implementation of hepatocellular carcinoma immunotherapy and accurate prognosis prediction.

The present invention provides a protein micro-array surface plasma resonance imaging detection system and its detection method. Said system includes surface plasma resonance micro-array protein sensor, incident arm, reflecting arm and signal processing unit, the described sensor includes prism, micro-array chip and sample cell, between prism and micro-array chip a refractivity oil layer is coated, the incident arm is positioned at one side of the sensor, successively includes semiconductor laser, collimator, polarizer, attenuator and reactangular light diaphragm, the reflecting arm is positioned at another side of the sensor, and successively includes lens and CCD receiver, and signal processing unit includes signal processing circuit and computer.

A system, method and device for the detection of reactions between analytes, (e.g., DNA, biomolecules, or cells) and a second compound are disclosed. The present invention includes a coating of a fluorescent material having a fluorescence that changes with temperature. The fluorescent material is associated with a substrate, and can be used for any type of surface reaction that requires determination of temperature conditions at an interface between the surface and the reaction analyte subject to assay. The substrate may be, for example, a microarray chip or microplate, preferably suitable for use in high-throughput screening of biomolecules or cells. Substrates containing the fluorescent material also can be used to compensate for temperature variations in refractive index in optical sensors.

Disclosed is a microarray chip for allergy-related immunoglobulin detection, especially for quantitative detection of total IgE and allergen-specific immunoglobulins (such as specific IgE, specific IgG, and specific IgM), which comprises a solid substrate, a reactive layer fabricated on the solid substrate, and at least one allergen or substance capable of binding to immunoglobulin of interest. Whereby, use the result of quantitative detection for allergen-specific IgE to determine hypersensitivity level. In addition, a method for allergy-related immunoglobulin detection using the microarray chip is present, which uses a secondary monoclonal antibody to minimize non-specific binding and applies an enzymatic reaction to amplify reaction signal. An efficient way is thus obtained, which not only reduces time consumption but also provides quantitative measurement.

The invention belongs to the technical field of chemical analysis and discloses a method for preparing a microarray chip between a hydrophilic phase and a hydrophobic phase and carrying out mass spectrum imaging quantitative analysis by using the microarray chip. The method comprises the following steps: selecting a hydrophobic polymer; printing the hydrophobic polymer on conductive glass by adopting a screen printing technique according to a designed template to form a hydrophilic region and a hydrophobic region which are clear and uniform; uniformly paving a water solution of a mixture system to be detected in the hydrophilic region to form a chip; drying and curing the chip; uniformly paving a base material solution for ionizing the water solution of the mixture system to be detected on the surface of the chip; after the chip is dried, forming a sample; measuring a mass spectrum image of the sample by using a mass spectrum imaging method; processing the image obtained by mass spectrum imaging of the sample in the same area through using a mathematical method, and carrying out the quantitative analysis. The method is high in automation degree, simple in operation and high in sensitivity; the detection sensitivity of a material to be detected reaches 10-15mol / L; the use amount of the sample is less and is as low as a microliter-grade.

The invention can prepare the microarray of double -stranded DNA(ds DNA) on the surface of solid substrates. By this method, the probes of ds DNA can engomphosised in the nuclear factor kappa B(NF-kappa B) to high-throughpput detect NF-kappa B inside of caryon and screen ds DNA / NF-kappa B molecules interfering dsDNA / NF-kappa B interaction. First, depending on patent technic and others methods of this invention, the invention prepare the microarray of double -stranded DNA(ds DNA) on the surface of solid substrates to allow mounts of dsDNA explorer on the dsDNA micro embattle to link with locus. Second, allow dsDNA micro embattle to across and link with specifically cell nuckoproteides or NF-kappa pure albumen. Third, do qualitative determination or quantiative determination to NF-kappa albumen in special caryon by reaction of NF-kappa B with dsDNA as micro embattle butt.

This invention relates generally to the field of microarray technology. In particular, the invention provides an integrated microarray device, which device comprises a substrate comprising a plurality of distinct microlocations and a plurality of microarray chips, wherein the number of said microlocations equals to or is more than the number of said microarray chips. In preferred embodiments, the devices also comprises a temperature controller at some or all of the microlocations. The use of the integrated microarray devices for detecting interactions among various moieties in various fields, such as clinical diagnostics, drug discovery, environmental monitoring and forensic analysis, etc., are further provided.

The nucleic acid sequencing process based on nucleic acid micro array chip is for sequencing genome and detecting gene transcription and expression spectrum of some specific species, and detecting various genome DNA modifying spectrum. Different DNA segments to be sequenced are fixed onto one solid chip to form nucleic acid micro array, and each of the DNA segments is made to contain the same common sequence as the complementary sequence of the sequencing primer. After crossing the common sequencing primer and the chip, some mixed nucleotide solution with certain ratio of fluorescent marker ddNTP and dNTP is injected to the chip to terminate on-chip base reaction with a micro flow controller by means of Sanger end terminating process. Once the termination is finished, one fluorescent signal is obtained through scanning to obtain the base extending information in different sample points on the chip. All the sequencing maps are finally superposed to obtain the complete nucleic acid segment information.

A fluidic nano / micro array chipset comprises a microarray filling chip and a nano / micro array stamping chip. There are a plurality of sample containers and a plurality of nano / micro channels on the top of the microarray filling chip, and a plurality of nano / micro-scaled micro filling holes on the bottom of the microarray filling chip. Each nano / micro channel is connected to one of the sample containers and leads the sample solution in that sample container to the corresponding micro filling hole. The nano / micro array stamp chip comprises a plurality of stamping heads arranged in an array pattern, with a body part of the stamp chip and a plurality of space channels forming hydrophobic areas. Each sample solution is stored in the body of the stamp chip, and is transported by the corresponding stamping head to the stamping part of this stamping head.

A computer based method for analyzing microarray chip information. A computer implemented artificial neural network (ANN) is trained by back propagation of error using a set of training microarray chip input vectors to create a trained ANN. At least one set of test data is applied to the trained ANN to generate a prediction. The trained ANN numerically analyzing with respect to a subset of the input vectors to identify those elements of the input vector which are most effective in obtaining the prediction. the set of input vectors is reduced in dimension to contain data only from those genes found most effective in obtaining the prediction to form a dimensionally reduced set of input vectors. The neural network is retrained using the dimensionally reduced set of input vectors by back propagation of error to generate a retrained network. The at least one set of test data is reapplied to the retrained neural network to generate a second prediction.

The invention discloses a hybridization system for real-time monitoring parsing process of a micro array chip, which comprises a hybridization cavity body, a temperature control device, a peripheral flow path and a real-time imaging module, wherein the hybridization cavity body is composed of the micro array chip, a cavity body cofferdam and a cover plate, and a fluid inlet and a fluid outlet are formed on the cover plate; the temperature control device is connected with the hybridization cavity body; the peripheral flow path is communicated with the hybridization cavity body by the fluid inlet and the fluid outlet; and the real-time imaging module comprises a light source and a photodetection device, and the light of the light source irradiates the surface of the micro array chip, reflected by the surface of the micro array chip and then projected to the photodetection device. The micro array chip hybridization system can realize dynamic hybridization, cleaning, drying and other flows of the chip; and the real-time imaging module can realize real-time monitoring on the magnetic bead intensity of the processing flows of the chip. According to the signal change condition, the chip processing condition is optimized; and the hybridization system can also be used for studying the dynamic problems of solid-liquid phase hybridization.

The present application describes a method for normalizing for variations in signal intensity observed in a biomolecular binding assay carried out in a flow cell cartridge. Variations in signal intensity occur as a result of the effect of the surfaces of a flow cell cartridge on the laminar flow of reagent through the cartridge. In any individual reagent stream, fluid flows faster in the center of the stream and slower at the outer periphery of the stream due to contact of the reagent with the walls of the cartridge, creating a parabolic fluid flow profile. The present invention describes a method for normalizing or calibrating out the differences in intensity observed in different regions of interest on a single chip or similar reactions carried out in different cartridges, as a result of these differential fluid flow rates. Microarray chips having integrated calibration regions are also described.

The invention discloses a semiconductor chip used for characteristic spectrum discrimination and a manufacture method thereof, as well as a characteristic spectrum discrimination device and a biochip scanning device using the chip to measure absorption spectroscopy and fluorescence spectrum. According to the invention, the chip used for characteristic spectrum discrimination comprises an array semiconductor optical sensor, the array structure of which comprises at least a detection pixel and at least a reference pixel; the chip also comprises at least one optical filter plate, and the surface of each detection pixel is covered by a corresponding optical filter plate. Each optical filter plate has characteristic wavelength, thus leading the wavelength which takes the characteristic wavelength as the center in a predetermined width range to transmit through the optical filter plate, while the wavelength beyond the width range not to transmit. According to the invention, the semiconductor technology can be used for manufacturing the chip for characteristic spectrum discrimination at low cost and an absorption spectroscopy apparatus, a fluorescence spectrum apparatus and a biological microarray chip, with small volume, can be conveniently manufactured at low cost.

The invention discloses a preparation method of a microarray chip. The method comprises the steps of target chip selection, auxiliary chip selection, chip docking, chip separation and the like, mother liquor droplets and buffer solution droplets of the surface of a target chip and the surface of an auxiliary chip are manipulated to be fused and split, the components in corresponding droplets are changed, and the obtained target chip is the microarray chip, target droplets are adsorbed on the hydrophilic region of the microarray chip, and the target droplets serve as a droplet array composed of multiple droplets with different components. According to the preparation method of the microarray chip, multiple droplets can be manipulated simultaneously to conduct fusion and separation, the droplet microarrays of concentration gradient, mechanical gradient, multicomponent chemistry, cell density, anisotropic gel and the like are formed, and broad prospects of preparation aspects on biological analysis and gel droplets are achieved.

This invention relates to a plastic substrate for a microarray chip, which is characterized in that an aminoalkylsilane with an aldehyde group derived from glutaraldehyde and introduced onto an amino group of the aminoalkylsilane exists on a surface of the plastic substrate, and also to a process for its production and a method of its use. Substrates according to the present invention have no variations in the immobilized amount of DNA fragments upon immobilization of DNA fragments, permit reproducible hybridization with high efficiency, and are excellent in storability.

A method for indexing and coding element information of at least one biological element provided on a microarray chip thereby identifying the microarray chip. Some of the element spots arranged on the microarray chip are used as index spots to be selectively provided with at a detective colorant. By doing so, the microarray chip is specified, and the element information as well as experimental information of the chip is automatically managed.

The invention relates to a chip automatic loading device of a microarray chip scanner, which is characterized by comprising a clamping box, a clamping box inlet and outlet device and a positioning bin, wherein a microarray chip is arranged in the clamping box, and when the microarray chip scanner is in a non-working state, the clamping box is placed outside a scanning platform of the microarray chip scanner; the clamping box inlet and outlet device is connected with the positioning bin, and can be used for automatically loading the clamping box in the positioning bin; and the positioning bin is arranged above the scanning platform of the microarray chip scanner, and used for positioning the working surface of the microarray chip in the clamping box. The invention not only can effectively protect the microarray chip from being damaged and polluted, but also has the characteristics of simple structure and low cost, thereby being capable of being widely applied to various microarray chipscanners.

The invention discloses a surface patterning-modified substrate. The surface patterning-modified substrate comprises a substrate and a patterned nano thin layer, wherein the surface of the substrate is provided with hydrophilic groups, and the nano thin layer is a PDMS thin layer with thickness being smaller than 1 micron; the patterned nano thin layer and the substrate are bonded so as to form a hydrophilic region without the nano thin layer and a hydrophobic region with the nano thin layer, the hydrophilic region is used for adsorbing microfluid, the microfluid is water, a solution or a suspension, and the shape of a projection of the microfluid on the surface of the substrate is identical with the corresponding hydrophilic region. The invention also discloses a preparation method for the substrate and application of the substrate in the preparation of array chips. According to the surface patterning-modified substrate and the preparation method therefor, the surface of the substrate is subjected to patterning modification by using a simple and fast process, and the substrate has a broad application prospect in the preparation of microarray chips.

The invention relates to a method for detecting an induced pluripotent stem cell in the technical field of detection, which comprises the following steps of: preparing magnetic nano-particles and water-soluble quantum dots; generating silicon dioxide microspheres by hydrolyzing tetraethyl orthosilicate and preparing fluorescent magnetic nano silicon spheres by adopting an inverse microemulsion method; carrying out surface modification on the fluorescent magnetic nano silicon spheres and then coupling an antibody to obtain the fluorescent magnetic nano silicon spheres coupled with the antibody; preparing a microarray chip by using internal control protein as a positive control, using bovine serum albumin as a negative control and utilizing the antibody for resisting a specific marker expressed on the surface of the induced pluripotent stem cell; and detecting a sample by utilizing the microarray chip and then carrying out fluorescence labeling on the microarray chip by utilizing the fluorescent magnetic nano silicon spheres coupled with the antibody to obtain a detecting result. The method of the invention can realize the simultaneous detection of a plurality of specific molecules on a single sample and has little sample usage quantity, high sensitivity and specificity, low detecting cost, simple operation, high automation degree and high detecting speed.

The present invention relates to a method of detecting a chromosome abnormality and a microarray chip for detecting a chromosome abnormality. More specifically, the present invention is directed to a method of detection for chromosome abnormality in order to easily diagnosing the health condition and disease of the subject by detecting of the chromosome abnormality rapidly and precisely, and to providing a probe for diagnosis of disease by identifying chromosome abnormality specific to the disease.

A process for high-flux screening, capturing and separation of target moleculae from complex substance (Chinese medicine or chemical mixer) includes preparing a ds DNA microarray chip to make its probe have a binding sites of dsDNA bindin, measuring the affinity of the sites, creating the standard affinity parameter system, mixing the microarray chip with said complex substance, reacting, detecting the affinity variation, screening the varied targets, linking them to the surface of chromatographic column, filling the complex substance in it, capturing the effective moleculae by probes, eluting and collecting the effective moleculae.

The invention provides methods and compositions for use in the diagnosis and management of cancer, particularly breast cancer. The invention utilizes differential gene expression profiles in tumor associated stroma and normal stroma to compile a stroma derived prognostic predictor that classifies breast cancer patients according to clinical outcome. The application provides nucleic acids, antibodies, microarray chips and kits for use with the methods described in the application.