31 results about "Microarray cgh" patented technology

The invention belongs to the field of biomedical detection, and particularly relates to a protein chip, a detection kit based on a polypeptide coding microarray chip and application of the detection kit. According to the protein chip provided by the invention, an antibody and a bispecific antibody of corresponding indexes can be added into a treating fluid according to detection requirements, the antibody and the bispecific antibody are combined with the chip in a manner of polypeptide specific recognition after being subjected to antigen specific recognition respectively, and joint detection of different antigen combinations and detection of a single index are carried out. By utilizing the polypeptide sample application chip and the antigen and antibody combined bispecific antibody provided by the invention, the universality of the chip in use, the high efficiency of detection and the low cost can be remarkably improved.

The invention relates to a detection method, in particular to a method for rapidly detecting alpha and beta-lactoglobulin in auxiliary material lactose based on a microarray chip technology. On the basis of an indirect competitive enzyme-linked immunosorbent assay principle and a silver enhanced developing technology, a high-sensitivity microarray chip detection platform is successfully constructed by virtue of a biological chip scanner, and rapid quantitative analysis of alpha-lactalbumin and beta-lactoglobulin in the auxiliary material lactose for injection is realized. According to the method, an antigen-antibody specific recognition mechanism and a nano-silver signal amplification mechanism are integrated, and the detection efficiency and sensitivity are remarkably improved through the high-throughput layout of the microarray chip. In the aspect of methodology performance, the technology has the remarkable advantages of simple and convenient operation process, short detection time, high sample treatment flux, excellent specificity (CRlt; 1%) and the like.

The invention belongs to the field of neuroblastoma tissue analysis, and particularly discloses a child neuroblastoma tissue microarray chip and a preparation method thereof.The child neuroblastoma tissue microarray chip comprises a glass slide base material, and a plurality of neuroblastoma tissue samples arranged in a dot-matrix mode are arranged on the glass slide base material; by integrating a plurality of neuroblastoma samples including various pathological typing, grading and clinical staging types of neuroblastoma in a tissue block, efficient sample processing and analysis are achieved, comprehensiveness and representativeness of research are ensured, a large number of samples are analyzed simultaneously through a microarray technology, and the accuracy and accuracy of analysis are improved. The requirements for high-throughput screening of specific genes and expression proteins thereof in scientific research activities are met, the prediction capacity for tumor prognosis and the guidance accuracy for treatment schemes are improved, and neuroblastoma tissues are analyzed more efficiently and comprehensively.

The invention discloses a surface enhanced Raman scattering (SERS) analysis platform for detecting early gastric cancer protein biomarkers as well as a preparation method and application thereof, and belongs to the technical field of detection. The preparation method comprises the following steps: (1) modifying the surface of Au (at) Pt nano particle (Au (at) PtNPs) nano enzyme with DNA1 (hpDNA1) of a hairpin structure to form two SERS probes; (2) modifying hpDNA2 on the surface of the special-shaped nanorod array (Au / SiNPA) to serve as a capture substrate; (3) assembling a 4 * 4 microarray chip as an SERS (Surface Enhanced Raman Scattering) analysis platform; the SERS analysis platform has the advantages of simplicity in operation, high throughput, low price, high sensitivity and specificity and the like; moreover, the repeatability is good, and large-scale preparation can be realized.

The application discloses a kind of based on digital droplet microfluidic multi-sample flux multi-index detection device and application thereof, belong to electromechanical device, microfluidic chip and immunoprotein analysis field.The device includes droplet microfluidic equipment and immunoprotein analysis chip component, droplet microfluidic equipment is used to drive multiple droplets, mainly divided into three major systems: power supply system, computer operating system and chip clamp system, immunoprotein analysis chip component includes biological analysis substrate and microarray chip.The present application drives micro liquid sample by digital droplet microfluidic technology, realizes the multi-index detection of automated immunoprotein analysis chip.The detection device of the present application has a series of advantages such as rapidity, independence, low consumption, automation, function integration and multi-index detection, and is expected to become the most fruitful rapid diagnostic device for improving tumor diagnosis positive rate.

The utility model discloses a draw-pull type shock absorption microarray chip detection kit which comprises a kit body, the kit body comprises a box body and a box cover covering the box body, a cooling box and a plurality of parallel microarray chip placing layers are sequentially arranged in the box body from bottom to top, an opening is formed in the front side face of the box body, and the microarray chip placing layers are arranged in the box body. The plurality of microarray chip placement layers and the cooling box can be pulled out from the opening in the front side surface; the adjacent microarray chip placing layers are connected through sponges, and the microarray chip placing layers and the cooling box are connected through sponges. The upper portion of the cooling box is open, an ice bag is arranged in the cooling box, cold air emitted by the ice bag is conducted upwards from the bottom through a hollow area in the sponge, and the refrigeration and heat preservation effects on the microarray chip are achieved. The utility model solves the technical problems that the kit is easy to break due to vibration in the transportation process, the insertion is inconvenient, and the temperature is not easy to control.

The invention discloses a DNA microarray chip with an ordered nanostructure and application thereof, the DNA microarray chip is mainly composed of an ordered silicon film array formed by self-assembly of SiO2 microspheres, the particle size of the silicon spheres is 250-650nm, a single-layer densely arranged ordered nanostructure is formed on the surface of a silicon wafer, and probes are distributed on the silicon wafer modified by an aldehyde group to form a spot array. The silicon film array is used as a substrate, SiO2 microspheres are self-assembled on a silicon wafer, the SiO2 microsphere array has optical characteristics, movement of DNA to a solid-liquid interface is accelerated, the probability of collision between DNA and probe DNA is increased, and finally DNA hybridization efficiency and imaging efficiency are improved.

Methods of detecting a leak from a subarray of a microarray chip, kits of components that facilitate leak detection, and microarray chips configured for leak detection are disclosed herein. The methods include positioning, within the given subarray, a sample solution, which includes dissolved sample molecules (DSMs) that define a dissolved sample oligonucleotide sequence and dissolved leak detection molecules (DLDMs) that define a predetermined dissolved leak detection oligonucleotide sequence. The methods also include detecting the DLDMs within a region of the microarray chip that is external the given subarray. The kits of components include the microarray chip and DLDMs. The microarray chips includes a plurality of subarrays and a leak detection region that is at least partially external the plurality of subarrays.

Use of a compound, microarray chip substrate, microarray chip and use and method thereof. The present application provides use of a compound, or a geometric isomer, tautomer, isotopically labeled, hydrate, solvate or salt thereof in chip surface modification. Also provided is a microarray chip substrate modified by the compound, and a microarray chip comprising the microarray chip substrate. The microarray chip substrate of the present application can greatly improve the surface modification function by surface modification, improve nucleic acid synthesis yield, amplify detection signal, and avoid non-specificity and cross-talk. It has a wide application prospect.

The present invention relates to a single nucleotide polymorphism marker for precocious puberty diagnosis or treatment prognosis prediction for the disease, and a use thereof. In the present invention, the DNA of 700 girl patients of precocious puberty is processed on a microarray chip containing a total of 8,754,887 SNP probes so that SNP genotype data is obtained, and 23 SNPs related to the risk of developing precocious puberty are discovered by means of genome-wide association analysis so that the SNPs can be used for precocious puberty diagnosis or treatment prognosis prediction, early diagnosis of precocious puberty can be conveniently and rapidly carried out, and the prediction of treatment effects and prognosis can be carried, and thus the present invention can be effectively used in a personalized treatment.

The invention discloses a molecular glue drug screening method and application in degradable drug screening. A molecular gel drug screening method comprises the following steps: preparing a compound library into a first microarray chip, and screening compounds combined with effect protein based on an OI-RD image to obtain a first candidate; preparing a second microarray chip, measuring binary binding kinetics of the first candidate and the effect protein based on an OI-RD optical real-time signal, and verifying to obtain a second candidate; and preparing a third microarray chip, and screening a compound subjected to the ternary reaction based on an OI-RD real-time signal of the ternary reaction. According to the screening method disclosed by the invention, by directly detecting the formation of the target protein-molecular glue-effect protein ternary complex, the screening accuracy is remarkably improved, the type of the molecular glue which is not directly combined with the target protein can be effectively identified, and molecular glue screening with higher specificity and higher flux is realized.

The application discloses a surface enhanced Raman scattering (SERS) analysis platform for detecting early gastric cancer protein biomarkers and a preparation method and application thereof, and belongs to the technical field of detection. The preparation method comprises the following steps: (1) modifying the surface of Au@Pt nano-enzyme (Au@Pt NPs) nano-enzyme with a hairpin structure DNA1 (hpDNA1) to form two SERS probes; (2) modifying hpDNA2 on the surface of a special-shaped nano-pillar array (Au / SiNPA) as a capture substrate; and (3) assembling a 4*4 microarray chip as a SERS analysis platform. The SERS analysis platform has the advantages of simple operation, high throughput, low price, high sensitivity and high specificity, and has good repeatability and can be prepared on a large scale.

The invention relates to the technical field of detection, and relates to a single-molecule protein detection method based on a microarray chip and a chemiluminescence immunoassay technology. An antigen-antibody complex reaction solution is randomly segmented into tens of thousands of microarray holes with the single-hole volume at the pL level by utilizing a microarray chip containing 0.5-200 thousands of micropores with the diameter of 5-30 [mu] m. Each micropore is used as an independent reaction unit for detection and comprises one or zero antigen-antibody compounds, a chromogenic substrate or exciting light is added to collect signal data in all the micropores, and the number of all the antigen-antibody compounds in a reaction system is directly and quantitatively detected according to a Poisson distribution calculation formula. And calculating the concentration of the antigen captured in the microarray chip. The method disclosed by the invention has the advantages of small background signal interference, high sensitivity, specificity, accuracy and precision, simplicity and rapidness in operation, low cost and obvious advantages in the technical field of single molecule detection, and the lower limit of detection reaches fg / ml.

The use of a compound, a microarray chip substrate, and a microarray chip and the use thereof and a method therefor. The present application provides a compound, or a geometric isomer, a tautomer, an isotope-labeled compound, a hydrate, a solvate or a salt thereof in the surface modification of a chip. Further provided is a microarray chip substrate modified by the compound, and a microarray chip comprising the microarray chip substrate.

The invention provides a microarray chip image identification method and system, and the method comprises the steps: positioning a Block external rectangular frame of an array through a Block target detection model, and enabling the Block target detection model to be generated through the training of a predetermined target detection model through an image set which is formed by a plurality of arrays; performing semantic segmentation on the Block bounding rectangular frame area by using a Spot semantic segmentation model to generate a spot image block mask image, and adjusting the angle of the Block bounding rectangular frame based on the projection result of the spot image block mask image; and calculating by using the spot image block mask graph to obtain the center-of-mass coordinate and radius of each spot area in the adjusted Block circumscribed rectangular frame, and finally determining the circumscribed circle of each spot image block. According to the method, the position of the Block can be accurately recognized, the Spot can be accurately aligned, meanwhile, the analysis efficiency is greatly improved, and the analysis time is shortened.

The embodiment of the invention discloses a manufacturing method of a pathological tissue microarray chip of a 3D cell model, a culture chip for preparing the 3D cell model is adopted for manufacturing, the culture chip is dried after being subjected to high-temperature and high-pressure sterilization, and low-adhesion treatment is performed for standby application; selecting a cell sample with a good growth state, and culturing a 3D cell model by utilizing the treated culture chip and the cell sample; directly performing embedding pretreatment on the 3D cell model in the culture chip to obtain a pre-embedded block comprising the 3D cell model; carrying out sample embedding treatment on the pre-embedded block by adopting OCT (Optical Coherence Tomography) glue to obtain a sample embedded block; and carrying out frozen section operation on the sample embedding block by adopting a freezing slicer to obtain the pathological tissue microarray chip. The steps of sample collection, transfer, centrifugation and the like can be omitted, the problems of sample loss, damage and the like are effectively avoided, the slicing operation is simplified, and the slicing efficiency is improved; besides, by utilizing array positioning of the culture micropores, the conditions of sample aggregation, deformation, extrusion damage, overlapping shielding and the like are avoided, and the requirements of visualization, data acquisition and statistics, spatial positioning and the like of sample monomers are met.

The utility model discloses a mixing carrier for sequencing a micro-array chip, which belongs to the field of biochips and comprises a bottom plate, hangers symmetrically arranged on two sides of the bottom plate, positioning grooves arranged on the bottom plate and pressing blocks arranged between the hangers and the bottom plate, and the positioning of the micro-array chip for sequencing is realized through the positioning grooves of the bottom plate. Fixing is achieved through the pressing blocks, the device can be fixed to a mixing instrument through the hanging lugs to conduct swing mixing, the structure is simple, operation is convenient, the reliability of micropore mixing can be ensured, the connecting grooves and the connecting protruding blocks are arranged on the periphery of the bottom plate, mixing operation can be conducted on multiple chips in a splicing mode, and the use flexibility is high.

The invention relates to the technical field of biomedicine, and particularly discloses application of an lncRNA marker based on m6A modification in prognosis prediction of colorectal cancer. Constructing a multi-dimensional prognosis evaluation model by integrating the lncRNA expression quantity, the m6A modification level and clinical pathological parameters; according to the technical scheme, the method comprises the steps that m6A modified lncRNA markers related to colorectal cancer prognosis are screened, the expression and modification states of the m6A modified lncRNA markers are quantitatively analyzed through qRT-PCR and m6A antibody detection technologies, a prognosis risk score is calculated in combination with clinical parameters, and accurate layering of the recurrence risk and survival time of a patient is achieved. The matched products comprise a detection kit, a microarray chip and data analysis software, are simple and convenient to operate and are suitable for clinical laboratories and third-party detection. The method breaks through the limitation of a traditional prognosis method, the prediction accuracy is remarkably improved, a scientific basis is provided for personalized diagnosis and treatment, and the method has wide clinical application prospects.

The present application relates to the quality inspection method of microarray chip scale production, comprising: step 1) the nanometer membrane is placed in clean environment, humidity <20%, temperature 23~25 ℃ for a period of time to make the nanometer membrane fully balanced;Step 2) preparation of sample solution, present preparation present use;Step 3) the nanometer membrane of step 1) is fully balanced, is placed in sample point instrument, is gradually sampled in the quality inspection point of nanometer membrane with the sample solution of step 2), each hole quality inspection point is not less than 2, 20nl / point;Step 4) the nanometer membrane of step 3) is sampled is loaded into the matching fixture, is placed under the lens of industrial camera, is photographed with industrial camera, is measured with software for each quality inspection point diameter and is exported;Step 5) analysis of the diameter data of each quality inspection point on the nanometer membrane, the overall standard deviation of the diameter of the quality inspection point on the nanometer membrane, the median and the range, the overall standard deviation ≤5%, the median 460 μm-480 μm, the range ≤30 μm is the nanometer membrane of quality inspection qualified.

The application relates to a microarray chip-based SERS serum analysis method, belonging to the cross field of medicine and artificial intelligence, and a technical scheme is as follows: by using the prepared microarray chip composed of AuNOs arrays, high-throughput spectrum acquisition can be realized, and the signal intensity of extremely weak biological components can be significantly amplified; SERS is combined with the microarray chip to overcome the problems of changes in spectrum peak position and intensity caused by the exposed environment in serum SERS detection; a spectrum recognition analysis model is proposed, which can be used for recognizing serum spectra at different stages, capturing and distinguishing several key spectral characteristics at different stages, effectively improving the accuracy, sensitivity and specificity of recognizing similar SERS spectra, so that the liver injury caused by cisplatin (CILI) can be accurately recognized and analyzed at the molecular level.

This application belongs to the field of biochemical substance detection, and particularly relates to a measuring device and its detection method. The measuring device includes a detection system, an SPRi system, a terahertz imaging system, and a data acquisition and control system. The measuring device incorporates a terahertz imaging system and sets four channels on the sample cell at the bottom of the microarray chip. A standard sample is sequentially introduced into the first channel, the analyte into the second channel, the standard sample into the third channel, and the analyte into the fourth channel. The data acquisition and control system controls the injection of different samples into the four channels, while simultaneously controlling the movement of the light source assembly and the terahertz emitter, so that the p-polarized beam and the terahertz signal simultaneously illuminate the same channel. By calculating the signals between different channels, the terahertz imaging system can determine the sample type within the fluid by measuring the refractive index change of the analyte compared to the standard sample, thereby improving the accuracy of determining the start and end times of specific binding.

The invention belongs to the technical field of biological sample treatment, and particularly relates to a wax block remelting method of a tissue microarray chip. According to the technical scheme, the method comprises the steps that a to-be-treated wax block is placed in a quartz glass mold; the lower layer of the wax block is preheated, the heating temperature is controlled to be 65-70 DEG C, the heating lasts for 2-10 min, and heating is stopped when the wax block on the bottom layer is dissolved by 1-2 mm; standing and cooling to form a bottom solidified wax layer; the mold filled with the wax is transferred to a cooling table, and the temperature of the cooling table is controlled to range from-10 DEG C to-5 DEG C; meanwhile, heating the upper layer of the wax block by adopting a heating source; when the upper-layer wax block is completely melted and a 1-2mm unmelted wax layer is reserved on the lower-layer wax block, removing bubbles in the wax liquid by using a fine needle; covering the bottom support of the embedding box, and continuously standing the wax block on the cold table until the wax block is completely cured. The method is mainly used for providing a new choice for the wax block remelting method of the tissue microarray chip.

The invention relates to a dual-channel microarray chip, a signal probe and a detection method for detecting exosome RNA (Ribonucleic Acid), which are characterized in that specific replication is carried out on target RNA through LCR (Like, Chromatography and Regulation) amplification reaction so as to fulfill the aim of amplifying the number of target objects and amplifying signals; then combining a fragment obtained by amplification with gold-coated magnetic nanoparticles to construct an electrochemical signal probe; purifying the signal probe through a magnetic separation technology, and re-dispersing the signal probe in a PBS solution; and fixing the signal probe on the microarray chip. According to the present invention, with the cooperation of the dual-channel switching assembly, the electrochemical detection system and the matched software control platform, the synchronous detection and analysis of the dual-target exosome RNA can be achieved, and the high detection sensitivity and the high specificity are provided so as to significantly improve the accuracy and the result reliability in the early screening and diagnosis.

The invention discloses a method for preparing coding microspheres based on a microarray chip and a product and application thereof, and the method comprises the following steps: spraying a coding sequence to the surface of a glass slide to construct a coding microarray chip; arranging the gel microspheres fixed with the amplification primers on the surface of a coding microarray chip in a single layer, so that the microspheres are tightly combined with the surface of the chip; copying a coding sequence on the surface of the chip to the surface of the microsphere by using a molecular hybridization and extension technology; eluting the microspheres with the copied and transferred coding sequences from the surface of the chip, carrying out bridge type PCR amplification reaction, and copying a large amount of the coding sequences transferred from the surface of the chip until the whole microspheres are full of the coding sequences; and after amplification is completed, one complementary chain is cut off, so that the complete coding microspheres are obtained. The chip can be reused, the cost is obviously reduced, the operation is simple, convenient and flexible, and the mass production is high.

The invention relates to a single molecule rapid detection method and system. The single molecule rapid detection method comprises the following steps: placing a to-be-detected microarray chip subjected to an in-situ reaction on a detection table of the detection system; the detection system turns on a bright field light source; the fluorescence camera starts an automatic focusing function, and automatic focusing is completed within 1 s; the fluorescence camera locks a focusing position parameter; the detection system turns off the bright field light source and turns on the fluorescence excitation light source; controlling the fluorescence camera to perform exposure, and collecting a fluorescence image; inputting the fluorescence image into a deep learning model, and identifying and outputting position information of all single-molecule fluorescence spots in the fluorescence image; counting the number of light spots falling into each unit on the microarray chip; on the basis of the Poisson distribution principle, the number of single molecule spots obtained through statistics serves as a direct counting result, and the absolute concentration or the absolute copy number is calculated. According to the invention, rapid, high-precision and absolute quantitative single molecule detection is realized.

The invention discloses an electronic microarray chip detector liquid path system and a control method, and belongs to the technical field of biological detection.The electronic microarray chip detector liquid path system comprises a four-channel-structure liquid distribution needle set, a liquid distribution needle set and a liquid distribution needle set, four channels of the rotary valve with the ten-channel structure are connected with the reagent needle, and the reagent needle needs to suck an air column larger than or equal to 2 mm after sucking a reagent so as to suck another reagent, so that physical isolation is realized; according to the confluence plate assembly adopting laser bonding, a micro-channel has a rectangular cross section (0.5 * 0.5 mm, the surface roughness Ra is less than or equal to 1.6 mu m), and the dead volume is less than or equal to 12 mu L; the temperature control unit is integrated on the bus board assembly and comprises an FPC (Flexible Printed Circuit) heating sheet and a temperature sensor; and the injection pump is linked with the flow sensor. Through three-stage cooperative control of a rotary valve, a confluence plate assembly and an injection pump, flow channel design and process optimization are combined, and accurate control over micro-upgrading liquid is achieved.

The invention discloses a polymer activated ester microarray chip as well as a preparation method and application thereof, and relates to the technical field of microarray chip preparation. The invention provides a poly (activated ester) microarray chip and a preparation method and application thereof, a poly (lysine) glass slide is modified by dicarboxylic acid activated ester, the poly (activated ester) microarray chip with a three-dimensional substrate is constructed, the process is simplified through a one-step coupling reaction, the technical bottleneck of a traditional microarray chip is broken through, and the production cost is reduced. The method has the advantages of simplicity and convenience in operation, high fixing efficiency and ultra-sensitive detection capability, provides an efficient tool for high-throughput analysis in the field of biomedicine, and has remarkable scientific value and market application prospect.

The application belongs to the technical field of biology, and discloses a microarray chip, a preparation method and application thereof. The microarray chip has hydrophilic reaction holes and hydrophobic non-reaction areas, the depth of the reaction holes is 20-50 mu m, the etching depth is much higher than the depth of 3-10 mu m of traditional laser etching, the hydrophobic angle of the non-reaction areas is more than 110 degrees, and the chip surface is flat and smooth. The application adopts double photolithography and surface hydrophobization treatment, and can quickly prepare the microarray chip with multiple hydrophilic reaction holes and hydrophobic non-reaction areas, which meets the needs of large-scale industrial production; different patterned masks can be designed to obtain reaction holes with different shapes for different detection scenes.

The application discloses a screening method of molecular glue drugs and application in degradation drug screening. The screening method of molecular glue drugs comprises the following steps: preparing a first microarray chip from a compound library, screening compounds combined with an effector protein based on an OI-RD image to obtain first candidates; preparing a second microarray chip, measuring binary binding kinetics of the first candidates and the effector protein based on OI-RD optical real-time signals, verifying, and obtaining second candidates; and preparing a third microarray chip, screening compounds for ternary reactions based on OI-RD real-time signals of ternary reactions. The screening method directly detects the formation of a target protein-molecular glue-effector protein ternary complex, significantly improves screening accuracy, can effectively identify molecular glue types that do not directly bind to target proteins, and realizes higher specificity and higher flux molecular glue screening.

The invention discloses an electronic microarray chip detector, and belongs to the technical field of biological detection, the electronic microarray chip detector comprises a bottom plate and a shell assembly arranged on the bottom plate, and a plurality of assemblies are integrated in the shell assembly. The reagent bin assembly can be used for storing a NaCl solution, an SSC buffer solution and an enzyme digestion reagent; the power socket assembly is arranged on the side wall of the shell assembly and used for connecting an external power supply. The sampling needle assembly comprises a sampling needle and a driving mechanism and can accurately draw a reagent. The chip bin assembly can contain a detection chip and move to a detection station, a rotary valve is arranged in the chip bin assembly, the sampling needle is connected with an inlet of the rotary valve through an independent pipeline, and an outlet of the rotary valve is connected with a confluence plate. The pump assembly is provided with a three-way electromagnetic valve and an injection pump, the injection pump generates negative pressure, and the three-way electromagnetic valve injects reagents according to a time sequence. The control panel assembly is electrically connected with the multiple assemblies, receives voltage signals of the chip bin assembly and uploads the voltage signals to the industrial personal computer for analysis. The detector has the advantages of accurate sampling, efficient detection and high integration level, and can improve the accuracy and efficiency of qualitative detection of nucleic acid.