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41 results about "Specific igm" patented technology
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Rapid generation of t cell-independent antibody responses to t cell-dependent antigens
InactiveUS20090104221A1Easy to demonstrateLess immunogenicMicrobiological testing/measurementDrug screeningEndemic diseasesGerminal center
The present invention comprises the use of follicular dendritic cells (FDCs) or FDC-like cells to generate FDC-dependent, but T cell-independent, B cell responses to T cell-dependent antigens, with antigen-specific and polyclonal antibody production in ˜48 h. In another embodiment, a germinal center (GC) lymphoid tissue equivalent (LTE) was used to generate antigen-specific IgM, followed by switching to IgG. The GC LTE model can be used in vaccine assessment. Dual forms of immunogen were used in the GC LTE and in vivo. Dual immunogens resulted in rapid, specific IgM responses and enhanced IgG responses. This vaccine design approach can be used, for example, to provide rapid IgM protection (˜24-48 h) and high-affinity IgG more quickly in people moving to areas with endemic disease, or in people with T cell insufficiencies, who can be immunized to rapidly generate protective IgM.
Owner:SANOFI PASTEUR VAX DESIGN +1
Microarray chip for detection of immunoglobulin
InactiveUS20060008895A1Determine hypersensitivity levelTime-effectiveBioreactor/fermenter combinationsSequential/parallel process reactionsTotal igeSpecific igm
Disclosed is a microarray chip for allergy-related immunoglobulin detection, especially for quantitative detection of total IgE and allergen-specific immunoglobulins (such as specific IgE, specific IgG, and specific IgM), which comprises a solid substrate, a reactive layer fabricated on the solid substrate, and at least one allergen or substance capable of binding to immunoglobulin of interest. Whereby, use the result of quantitative detection for allergen-specific IgE to determine hypersensitivity level. In addition, a method for allergy-related immunoglobulin detection using the microarray chip is present, which uses a secondary monoclonal antibody to minimize non-specific binding and applies an enzymatic reaction to amplify reaction signal. An efficient way is thus obtained, which not only reduces time consumption but also provides quantitative measurement.
Owner:CHENG LOONG CORPORATION
Colloidal gold method detection test strip and reagent kit for IgM and IgG antibodies of mycoplasma pneumoniae and preparation method of reagent kit
The invention discloses a colloidal gold method detection test strip and a reagent kit for IgM and IgG antibodies of mycoplasma pneumonia (MP) and a preparation method of the reagent kit. The test strip determines the IgM antibody and the IgG antibody by using a principle of an immunocapture method; the specific IgM and IgG antibodies of the MP can be detected jointly by one operation; the operation process is simplified; and the reagent kit is simple, convenient, rapid and accurate in detection, is particularly applicable to primary screening and epidemiological survey, and has an auxiliary diagnosis effect on early and interim mycoplasma pneumoniae infection.
Owner:JIANGSU KEYGEN BIOTECH CORP LTD
Colloidal gold test strip and test strip card for detecting IgM antibody, and preparation and detection method
InactiveCN105259345AThe detection process is fastImprove efficiencyBiological testingParainfluenza virus antigenIgm antibody
The invention provides a colloidal gold test strip for detecting an IgM antibody. The IgM antibody is a specific IgM antibody for nine respiratory tract infection pathogens, and the colloidal gold test strip comprises a sample pad, a conjugate pad, a nitrocellulose film and a water absorption pad which are attached to a polyvinyl chloride base plate in sequence; the conjugate pad is a glass fiber film wrapped with a rabbit-anti-human IgM antibody-colloidal gold conjugate; the nitrocellulose film is wrapped with 9 detection lines and 1 quality control line in sequence; the 9 detection lines are respectively a mycoplasma pneumoniae recombined antigen, a chlamydia pneumoniae recombined antigen, an influenza a virus antigen, an influenza B virus antigen, a sendai virus antigen, a legionella pneumophila antigen, a Coxiella burnetii antigen, a respiratory syncytial virus antigen and an adenovirus antigen, and the quality control line is a second antibody. The invention further provides a colloidal gold test strip card comprising the colloidal gold test strip and a colloid gold kit, a preparation method of the colloidal gold test strip card, and a method for realizing detection by adopting the colloidal gold test strip, the test strip card or the kit.
Owner:THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
Colloidal gold chromatographic band for joint detecting specific IgM. IgG antibody and producing method thereof
The invention provides a colloidal gold chromatography band and the preparing technology to detect the special IgM,IgG. The colloidal gold is as the labeled thing of the colloidal gold. One end of the PVC back plate is pasted with the sample pad, the compound pad, the nitryl fibrous membrane, the other end is pasted with the absorbing pad; the compound pad is the glass fibrous membrane covered with the antigen-colloidal gold compound. The character is in that: the fibrous membrane includes three cover lines which cover the anti IgM antibody, the special antigen and the antibody according to the antigen separately. The invention detects the IgM antibody using the immune capture method principle and detects the IgG antibody by the double antigen sandwich method. So it can detect the special IgM, IgG antibody by one operation and the result has the high whole according ratio.
Owner:北京英诺特生物技术股份有限公司
Reagent strip for joint detection of syphilis specific IgM and IgG antibodies and preparation method thereof
InactiveCN101825634AJoint detection implementationStrong specificityMaterial analysisReagent stripSyphilis
The invention provides a reagent strip for joint detection of a syphilis specific IgM and IgG antibodies and a preparation method thereof, relating to a reagent for detection of a syphilis specific antibody. The invention provides the reagent strip for joint detection of the syphilis specific IgM and IgG antibodies and the preparation method thereof. The reagent strip is provided with a vector plate, a loading pad, a colloidal gold pad, a nitrocellulose membrane, a syphilis specific IgG antibody detection line, a syphilis specific IgM antibody detection line, a contrast line and an absorption pad. The preparation method comprises the following steps: preparing sample application of nitrocellulose membranes for recombinant syphilis antigens TPN17 and TPN47, preparing colloidal gold, labeling syphilis specific antigen TPN17 and TPN47 with colloidal gold and preparing the reagent strip for joint detection of the syphilis specific IgM and IgG antibodies.
Owner:ZHONGSHAN HOSPITAL XIAMEN UNIV
Colloidal gold chromatography strip for detecting specific IgM antibody and method for making same
ActiveCN101013133AImprove anti-interference abilityImprove stabilityMaterial analysisGlass fiberCellulose
The invention provides a colloidal gold chromatography and preparation technology to detect specific IgM antibody. The chromatography uses colloidal gold as the marker, and uses immunohistochemistry capture method to detect and determine IgM antibodies, and in PVC backplanes, sequentially and mutually attached with sample pad, composite material pad, nitro cellulose membrane, absorbing pad; the nitro cellulose membrane includes the detection area and the control area, the characteristics being: the said composite material pad coated with antigen-colloidal gold composite glass cellulose membrane, and the said detection area covers the anti-IgM antibody, and the said control area covers the antibody corresponding to the antigen.
Owner:北京英诺特生物技术股份有限公司
Mycoplasma pneumoniae detection kit
InactiveCN105759034AQuick checkAccurate detectionBiological material analysisSerum igeImmunofluorescence
Owner:山东德诺生物科技有限公司
Reagent strip for joint detection of syphilis specific IgM antibody and specific total antibody and preparation method thereof
The invention provides a reagent strip for joint detection of a syphilis specific IgM antibody and a specific total antibody and a preparation method thereof, relating to a reagent for joint detection of a syphilis specific IgM antibody and a specific total antibody. The reagent strip is provided with two reagent strips, wherein the two reagent strips are both provided with vector plates, sample adding pads, colloidal gold pads, nitrocellulose membranes, control lines and absorbent pads and respectively provided with a syphilis specific IgM antibody detection line and a specific total antibody detection line. The preparation method comprises the following steps: preparing recombinant syphilis antigens TPN17 and TPN47, applying samples of nitrocellulose membranes, preparing colloidal gold,labeling TPN17 and TPN47 with colloidal gold and preparing immunochromatography detection strips. The reagent strip can be used for detecting the syphilis specific IgM antibody and the specific totalantibody in the specimens of whole blood, blood serum, blood plasma, cerebrospinal fluid and the like. During detection, the specimen amount is minimal, special instruments are not needed and the results are directly interpreted by the naked eyes. The reagent strip is simple, convenient, fast, accurate and reliable and has strong specificity, high sensitivity, low cost and wide application range.
Owner:ZHONGSHAN HOSPITAL XIAMEN UNIV
Preparation of Zika virus multi-segment fusion protein and IgG/IgM antibody detection kit
InactiveCN106841601AQuick checkDisease diagnosisAgainst vector-borne diseasesBiotin-streptavidin complexIgm antibody
The invention aims at providing a simple and quick Zika virus detection kit. The kit optimally selects fusion expression protein as diagnostic antigen; an anti-human IgG monoclonal antibody A374, an anti-human IgM monoclonal antibody A371 and a biotin-BSA conjugate are respectively coated on a nitrocellulose membrane as a detection line and a quality control line; colloidal gold labeled fusion expression protein and colloidal gold labeled streptavidin and other reagents are matched; and an immunochromatography capture method principle is used for qualitative detection of Zika virus specific IgM antibody and IgG antibody in human serum, thereby realizing quick and specific diagnosis of Zika virus infection.
Owner:GUANGZHOU DARUI BIOTECH
SERS-immunochromatography detection method for rapidly and highly sensitively detecting Mycoplasma pneumoniae infection
InactiveCN110133255AHigh detection sensitivityImprove the detection rateBiological testingSurface-enhanced Raman spectroscopyDTNB
The invention relates to a SERS-immunochromatography detection method for rapidly and highly sensitively detecting Mycoplasma pneumoniae infection. The detection principle is that Nitrocellulose membranes (NC) are used as a carrier, a double-layer dye 5,5'-dithiobis (2-nitrobenzoic acid){5,5'-dithiobis- (2-nitrozoic acid), DTNB} labeled Au@Ag nano material coupling detection antibody is used as anSERS probe, a novel surface enhanced Raman spectroscopy (SERS-ICA)-based immunochromatography technology is established by combining the traditional Immunochromatography assay (ICA), and the technology is used for detecting human IgM and Mycoplasma pneumoniae (MP) specific IgM positive serum samples. The detection comprises the processes of sample dilution, SERS probe release, antigen-antibody reaction, result analysis and the like. The SERS-immunochromatography detection method for rapidly and highly sensitively detecting Mycoplasma pneumoniae infection can improve the detection sensitivityof human IgM and the detection rate of the lung branch positive serum specimen, and has important significance for clinical treatment.
Owner:ACADEMY OF MILITARY MEDICAL SCI
Test paper strip for rapidly detecting brucellosis IgM antibody colloidal gold
InactiveCN101363862ASave manpower and material resourcesThe result is clear and easy to distinguishMaterial analysisBrucella IgMSpecific igm
The invention provides a test strip for rapid detection of a Brucella IgM antibody, which comprises a reaction film and a conjugate release pad. The reaction film has a detection band simultaneously coated with Brucella specific antigen bp26, and a quality control band coated with a double-antibody. The conjugate release pad is coated with colloidal golden labeled anti-human IgM monoclonal antibody. A membrane chromatography indirect sandwich method is adopted to detect the Brucella specific IgM antibody in a specimen. The test strip is simple in operation, convenient, and fast, and has the advantages of no requirements of special instruments and special training, clear and identified result, and easy popularization. The test strip is suitable for base course, site detection and early diagnosis, and has auxiliary effect on the diagnosis of Brucella infection.
Owner:BEIJING ZHUANGDI HAOHE BIOMEDICINE SCI & TECH
Western blot kit for cardiolipin and specific IgM antibodies of syphilis and preparation thereof
The invention provides a western blot kit for cardiolipin and specific IgM antibodies of syphilis and preparation thereof, relating to a kit. The kit is provided with a carrier plate, a cellulose nitrate membrane, a detection line of the specific IgM antibodies and cardiolipin IgM antibodies of the syphilis and a control line, wherein the detection line and the control line are arranged on the cellulose nitrate membrane in sequence; specific recombinant antigens and cardiolipin antigens of the syphilis are coated at the detection line of the specific IgM antibodies of the syphilis, and human IgM antibodies are coated at the control line; and horse radish peroxidase is marked on anti-human mu-chain antibodies. The preparation comprises the following steps: preparing the specific recombinant antigens of the syphilis, then preparing the cardiolipin antigens, carrying out sample application on the cellulose nitrate membrane, and preparing mu-chain monoclonal antibodies of anti-human IgM specific segments; and marking the horse radish peroxidase on the anti-human IgM specific segments mu-chain monoclonal antibodies, and then preparing the western blot kit. The kit provided by the invention can be used for detection of the specific IgM antibodies and cardiolipin antigens of the syphilis in specimens such as whole blood, blood serum, blood plasma, cerebrospinal fluid and the like.
Owner:ZHONGSHAN HOSPITAL XIAMEN UNIV
Method for detecting specificity of Epstein-Barr viruses through Epstein-Barr virus p18-p23 fused capsid antigen
The invention discloses a method for detecting specificity of Epstein-Barr viruses (EBV) through an Epstein-Barr virus p18-p23 fused capsid antigen. The method is characterized in that an overlap extension polymerase chain reaction (PCR) technology is adopted; a coding gene p23 and a coding gene p18 are fused in vitro by a coding sequence of an intermediate head (Gly4Ser)3; a fused protein is expressed and the expressed fused protein is purified and is subjected to specificity identification through an immunoblotting technology; the purified fused protein is coated on an enzyme-linked immuno sorbent assay (ELISA) reaction plate and component content and reaction conditions are optimized; commercial horseradish peroxidase-labelled goat anti-human IgM and IgG and a series of other reagents are prepared; and EBV-viral cuspid antigen (VCA) specific IgM and IgG indirect ELISA diagnostic kit is obtained by assembling.
Owner:邱清芳
High-throughput treponema pallidum specific antibody detection kit and preparation method thereof
ActiveCN104330562AAccurate detectionAccurate Epidemiological SurveyMaterial analysisSpiroplasmaSpecific igm
Owner:ZHONGSHAN HOSPITAL XIAMEN UNIV +1
Leptospira IgM antibody quick detection test strip
ActiveCN101592665ASave manpower and material resourcesThe result is clear and easy to distinguishMaterial analysisIgm antibodyColloidal gold
The invention provides a quick detection test strip for detecting leptospira IgM antibody, comprising a reaction film and a combination releasing pad; wherein the reaction film is provided with a detection zone capable of wrapping leptospira specific antigen ompL1 and LipL32 and a quality control zone encasing secondary antibody IgG, and the combination releasing pad is encased by anti-human IgM monoclonal antibody marked by colloidal gold. Leptospira specific IgM antibody in a specimen is detected by applying membrane chromatography capturing method. The test strip of the invention is applied to detection and is easy, convenient, fast and short-cut to operate, special instrument or equipment or professional training are not needed, the result is clear and easy to recognize, and the operation is easy, thus being easy to popularize, being applicable to basic level, field detection and early diagnosis, and being beneficial to leptospira infection diagnosis.
Owner:辽宁迪浩生物科技有限公司
Test paper strip for detecting encephalitis virus IgM antibody colloidal gold, method for making same and applications
ActiveCN101363866ASave manpower and material resourcesThe result is clear and easy to distinguishMaterial analysisCelluloseIgm antibody
The invention provides a test strip for rapid detection of Japanese encephalitis virus IgM antibody. A Japanese encephalitis virus E gene antigen domain III and a double-antibody IgM III coat a nitrate cellulose film (NC film), and a membrane chromatography indirect sandwich method is adopted to detect the Japanese encephalitis virus specific IgM antibody in an infected human body specimen in combination with a colloidal gold labeled antihuman IgM monoclonal antibody. The test strip is simple in operation, convenient, and fast, and has the advantages of no requirements of special instruments and special training, clear and identified result, and easy popularization. The test strip is suitable for base course, site detection and epidemiological investigation, and has auxiliary effect on the diagnosis of Japanese encephalitis virus infection.
Owner:辽宁迪浩生物科技有限公司
Arg-gly-asp (RGD)-fused porcine circovirus 2 (PCV2) virus-like particles (VLPs), mutant infectious clone and preparation method of RGD-fused PCV2 VLPs, and application of RGD-fused PCV2 VLPs and infectious clone
ActiveCN110669142AImprove the level ofEnhance immune responseAntibody mimetics/scaffoldsViral antigen ingredientsVirus-like particleSpecific igm
The invention discloses arg-gly-asp (RGD)-fused porcine circovirus 2 (PCV2) virus-like particles (VLPs), an infectious clone and a preparation method of the RGD-fused PCV2 VLPs, and application of theRGD-fused PCV2 VLPs and the infectious clone. According to the RGD-fused PCV2 VLPs, RGD oligopeptides are fused on PCV2 Cap proteins. According to the RGD-fused PCV2 VLPs, the PCV2 Cap proteins are subjected to RGD polypeptide sequence oligopeptide modification, the RGD oligopeptides are fused on the PCV2 Cap proteins, and the PCV2 VLPs with RGD-fused on the surfaces are assembled in vitro. Compared with wild PCV2 VLPs, the levels of PCV2 specific IgM and IgG antibodies generated by RGD-fused PCV2 VLP immune mice are significantly increased, high-titer PCV2 antibodies can be generated in an induced mode, and the RGD-fused PCV2 VLPs enhance humoral immunity response. The RGD-fused PCV2 VLPs can be applied to development of enhanced VLPs and differential diagnosis molecular label VLPs vaccines, and a new idea is provided for research and development of PCV2 vaccines.
Owner:湖南派智生物科技有限公司
Method for detecting nephrotic syndrome hemorrhagic fever antibody
The present invention provides a method for detecting hemorrhagic fever with renal syndrome (HFRS) antibody, belonging to the field of biological technology. It relates to a technique capable of utilizing immunomagnetic trapping technique and antigen protein of enzyme-labelled recombinant virus to detect antibody of HFRS due to Hantanvirus. Said method includes the following steps: purifying S gene recombinant protein of Hantanvirus representative strain Z10 and L99 expressed by pET prokaryotic expression system, labeling biotin or horseradish peroxidase, making magnetic microsphere be connected with sheep anti-human IgM (mu chain) or sheep anti-human IgG antibody and making it be sensitized to obtain immunomagnetic microsphere for detecting specific IgM / IgG antibody of seoul type or Hantan type of Hantanvirus and creating the quick and sensitive immunomagnetic microphere ELISA method for detecting HFRS specific IgM / IgG antibody in blood serum sample.
Owner:天津市卫生防病中心 +2
Identification and production of high affinity igm antibodies and derivatives thereof
InactiveUS20190183997A1High affinityCell receptors/surface-antigens/surface-determinantsDepsipeptidesAntigen Binding FragmentIgm antibody
Provided herein are methods of sorting antigen-specific IgM memory B cells (MBCs), compositions and methods comprising such antigen-specific IgM MBCs, and recombinant antibody or antigen-binding fragments isolated from such antigen-specific IgM MBCs. As demonstrated herein, IgM and IgD MBCs are unique populations of cells with distinct phenotypic, functional and survival properties. Accordingly, the antigen-specific IgM MBCs and antibodies and antigen-binding fragments derived from these cells described herein are useful in therapeutic applications in vaccine strategies and treatment of infectious diseases.
Owner:UNIV OF WASHINGTON +1
Combined detection card for simultaneously detecting specific IgM and IgG antibodies of ASFV as well as preparation method and application thereof
The invention provides a combined detection card for simultaneously detecting specific IgM (Immunoglobulin M) and IgG (Immunoglobulin G) antibodies of ASFV (African Swine Fever Virus). An IgM detection strip and an IgG detection strip are combined on one detection card. Each detection strip comprises a sample pad, a sample combination pad, a chromatography membrane and a water absorption pad. The two chromatography membranes adopt the same recombinant ASFV antigens as solid phase coating substances, namely MGF 360-14L, CD2v and p72. The sample combination pad of the IgM detection strip is coated with biotin-labeled anti-pig IgM, a tracer-labeled streptavidin and a tracer-labeled rabbit anti-chicken IgY; and the sample combination pad of the IgG detection strip is coated with biotin-labeled anti-pig IgG, a tracer-labeled streptavidin and a tracer-labeled rabbit anti-chicken IgY. The invention also provides a preparation method and application of the combined detection card. The detection card is prepared by using an immunochromatography technology, the operation is convenient, and the result is visual.
Owner:XINGJIA BIO ENG CO LTD
Kit and method for detecting novel coronavirus SARS-CoV-2 antibody
InactiveCN113295873ADetection speedLow technical requirementsChemiluminescene/bioluminescenceBiological testingSpecific igmCoronavirus antibody
The invention provides a kit for detecting a novel coronavirus SARS-CoV-2 antibody. The kit comprises magnetic particles coated with an SARS-CoV-2 antigen, an anti-human IgM / IgG secondary antibody labeled by a chemiluminescent marker, and a non-novel coronavirus antigen. By adopting the kit, whether a patient sample to be detected contains a specific IgM / IgG antibody generated by infection of the novel coronavirus SARS-CoV-2 or not can be specifically detected, and the specific IgM / IgG antibody does not generate cross reaction with influenza virus, common coronavirus and the like. The invention also provides a detection method of the novel coronavirus.
Owner:MEDCAPTAIN MEDICAL TECH
Method for preparing IgM (Immunoglobulin M) calibrator
PendingCN114544295AHigh affinityImprove stabilityPreparing sample for investigationIgm antibodyAntiserum
The invention discloses a method for preparing an IgM calibrator, and relates to the field of IgM calibrators, the method comprises the following steps: 1, obtaining a sheep polyclonal antibody: immunizing a sheep according to a fixed immunization program, and extracting serum when the titer is qualified; 2, purifying the sheep polyclonal antibody: purifying the extracted sheep polyclonal antibody serum by using affinity chromatography; 3, cutting the sheep polyclonal antibody: cutting the sheep polyclonal antibody IgG by using an enzyme, purifying a Fab fragment in the sheep polyclonal antibody, and using the purified Fab fragment for subsequent coupling; 4, coupling of the human IgM and the Fab fragment: coupling the human IgM and the Fab fragment of the sheep polyclonal antibody by using a chemical method; 5, detection: carrying out affinity test and aging test on a product obtained by coupling the human IgM and the Fab fragment of the sheep polyclonal antibody; the prepared specific IgM is added into negative serum according to a certain concentration to obtain the IgM antibody serum, and the IgM antibody serum can be used for research and development of in-vitro diagnosis IgM kits and calibration products of the specific IgM serum.
Owner:武汉原谷生物科技有限责任公司
Method for making recombinant antigen pp 65 envelope enzyme-linked reaction plate and ELISA test kit
The present invention relates to a new method for making recombinant antigen PP65 coated enzyme labeled reaction plate and ELISA kit. Its key technique mainly liesin that using gene engineering method to prepare specific recombinant antigen-HCMV pp65, after the antigen is exracted and purified, using the coating plate as important component in the kit, besides, after the anti-human IgM monoclone antibody prepared by utilizing mouse-mouse hybridome technique is labeled by using horseradish peroxidase, it can be used for assembly of kit. Said method capable of creating sensitive quick ELISA antibody to trap HCMV specific IgM antibody in serum has high specificity and high stability, and said kit mainly can be used in laboratory research and clinical diagnosis.
Owner:王明丽
A homogeneous immunoassay kit for detecting target igm antibody in a sample and its use method and application
Owner:CHEMCLIN DIAGNOSTICS CO LTD
Babesia mocroti 2D97 antigen protein and application thereof
ActiveCN108893476ALower immune responseImprove throughputMicrobiological testing/measurementImmunoglobulinsEscherichia coliNucleotide
The invention discloses a babesia mocroti 2D97 antigen protein and application thereof. The protein has an amino acid sequence of SEQ ID NO:2 as shown in the specification, and has an encoded gene sequence of SEQ ID NO:1 as shown in the specification. An immunoreaction spectrum of babesia mocroti is obtained, results show that in a specific IgG antibody reaction, only one antigen protein 2D97 which has relatively intense immunoreactions with early-stage mouse serum of 3dpi and 7dpi is screened, and the antigen protein can be a candidate for early-stage antigen diagnosis. Escherichia coli prokaryotic expression is carried out with the nucleotide sequence of the antigen protein 2D97, a recombinant protein of escherichia coli is successfully obtained, and the protein has relatively intense immunoreactions with early-stage mouse serum of 3dpi and 7dpi in protein chip analysis of a specific IgM antibody, and can be applied to detection or diagnosis at an early stage and / or window stage within 3-7 days after infection of babesia mocroti.
Owner:中国疾病预防控制中心寄生虫病预防控制所国家热带病研究中心 +1
Colloidal gold chromatography strip for detecting specific IgM antibody and method for making same
ActiveCN101013133BImprove anti-interference abilityImprove stabilityMaterial analysisGlass fiberCellulose
The invention provides a colloidal gold chromatography and preparation technology to detect specific IgM antibody. The chromatography uses colloidal gold as the marker, and uses immunohistochemistry capture method to detect and determine IgM antibodies, and in PVC backplanes, sequentially and mutually attached with sample pad, composite material pad, nitro cellulose membrane, absorbing pad; the nitro cellulose membrane includes the detection area and the control area, the characteristics being: the said composite material pad coated with antigen-colloidal gold composite glass cellulose membrane, and the said detection area covers the anti-IgM antibody, and the said control area covers the antibody corresponding to the antigen.
Owner:北京英诺特生物技术股份有限公司
Babesia voles 2d97 antigen protein and its application
ActiveCN108893476BLower immune responseImprove throughputMicrobiological testing/measurementImmunoglobulinsEscherichia coliNucleotide
The invention discloses a Babesia vole 2D97 antigen protein and application thereof. Its amino acid sequence is shown in SEQ ID NO: 2, and its coding gene sequence is shown in SEQ ID NO: 1. The present invention obtains the immune response spectrum of Babesia voles, and finds that in the specific IgM antibody response, only an antigen protein 2D97 which exhibits a higher immune response with the early 3dpi and 7dpi mouse serum is screened, which can be used as an early diagnosis. Antigen candidates. The nucleotide sequence of the antigenic protein 2D97 was used for prokaryotic expression in Escherichia coli, and its recombinant protein was successfully obtained. In the protein chip analysis of specific IgM antibody, it showed that it was higher than the early 3,7dpi mouse serum. The immune response can be used for detection or diagnosis in the early and / or window period of Babesia voles infection 3 to 7 days.
Owner:中国疾病预防控制中心寄生虫病预防控制所国家热带病研究中心 +1
Novel coronavirus COVID-2019 detection card and preparation method thereof
PendingCN111474352AImprove accuracyEasy to detectBiological material analysisAgainst vector-borne diseasesSpecific iggEngineering
The invention discloses a novel coronavirus COVID-2019 detection card and a preparation method thereof. The novel coronavirus COVID-2019 detection card comprises a card supporting layer attached to detection test paper, a sample unit positioned on the card supporting layer, a preprocessing unit positioned on the card supporting layer, a marker unit positioned on the card supporting layer, a detection unit positioned on the card supporting layer, and a recycling unit positioned on the card supporting layer; the sample units correspond to the sample holes; the pretreatment unit corresponds to the pretreatment hole; a hollow or transparent sealed detection window is arranged at the position, corresponding to the detection unit, of the shell; the erythrocyte agglutination layer is provided with erythrocyte agglutinin; the endogenous interfering substance treatment layer is provided with an endogenous interfering substance conjugate; the exogenous interfering substance treatment layer is provided with an exogenous interfering substance chelating agent; a non-specific IgM antibody treatment layer is provided with an IgM chelating agent; a non-specific IgG antibody treatment layer has anIgG precipitant. The problem that interfering substances influence the detection result of the novel coronavirus is solved, and the detection accuracy is improved.
Owner:北京乐普诊断科技股份有限公司
Test paper strip for detecting encephalitis virus IgM antibody colloidal gold, method for making same and applications
ActiveCN101363866BSave manpower and material resourcesThe result is clear and easy to distinguishMaterial analysisCelluloseIgm antibody
The invention provides a test strip for rapid detection of Japanese encephalitis virus IgM antibody. A Japanese encephalitis virus E gene antigen domain III and a double-antibody IgM III coat a nitrate cellulose film (NC film), and a membrane chromatography indirect sandwich method is adopted to detect the Japanese encephalitis virus specific IgM antibody in an infected human body specimen in combination with a colloidal gold labeled antihuman IgM monoclonal antibody. The test strip is simple in operation, convenient, and fast, and has the advantages of no requirements of special instruments and special training, clear and identified result, and easy popularization. The test strip is suitable for base course, site detection and epidemiological investigation, and has auxiliary effect on the diagnosis of Japanese encephalitis virus infection.
Owner:辽宁迪浩生物科技有限公司
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