41 results about "Specific igm" patented technology

The present invention comprises the use of follicular dendritic cells (FDCs) or FDC-like cells to generate FDC-dependent, but T cell-independent, B cell responses to T cell-dependent antigens, with antigen-specific and polyclonal antibody production in ˜48 h. In another embodiment, a germinal center (GC) lymphoid tissue equivalent (LTE) was used to generate antigen-specific IgM, followed by switching to IgG. The GC LTE model can be used in vaccine assessment. Dual forms of immunogen were used in the GC LTE and in vivo. Dual immunogens resulted in rapid, specific IgM responses and enhanced IgG responses. This vaccine design approach can be used, for example, to provide rapid IgM protection (˜24-48 h) and high-affinity IgG more quickly in people moving to areas with endemic disease, or in people with T cell insufficiencies, who can be immunized to rapidly generate protective IgM.

The invention discloses a colloidal gold method detection test strip and a reagent kit for IgM and IgG antibodies of mycoplasma pneumonia (MP) and a preparation method of the reagent kit. The test strip determines the IgM antibody and the IgG antibody by using a principle of an immunocapture method; the specific IgM and IgG antibodies of the MP can be detected jointly by one operation; the operation process is simplified; and the reagent kit is simple, convenient, rapid and accurate in detection, is particularly applicable to primary screening and epidemiological survey, and has an auxiliary diagnosis effect on early and interim mycoplasma pneumoniae infection.

The invention provides a colloidal gold test strip for detecting an IgM antibody. The IgM antibody is a specific IgM antibody for nine respiratory tract infection pathogens, and the colloidal gold test strip comprises a sample pad, a conjugate pad, a nitrocellulose film and a water absorption pad which are attached to a polyvinyl chloride base plate in sequence; the conjugate pad is a glass fiber film wrapped with a rabbit-anti-human IgM antibody-colloidal gold conjugate; the nitrocellulose film is wrapped with 9 detection lines and 1 quality control line in sequence; the 9 detection lines are respectively a mycoplasma pneumoniae recombined antigen, a chlamydia pneumoniae recombined antigen, an influenza a virus antigen, an influenza B virus antigen, a sendai virus antigen, a legionella pneumophila antigen, a Coxiella burnetii antigen, a respiratory syncytial virus antigen and an adenovirus antigen, and the quality control line is a second antibody. The invention further provides a colloidal gold test strip card comprising the colloidal gold test strip and a colloid gold kit, a preparation method of the colloidal gold test strip card, and a method for realizing detection by adopting the colloidal gold test strip, the test strip card or the kit.

The invention provides a colloidal gold chromatography band and the preparing technology to detect the special IgM,IgG. The colloidal gold is as the labeled thing of the colloidal gold. One end of the PVC back plate is pasted with the sample pad, the compound pad, the nitryl fibrous membrane, the other end is pasted with the absorbing pad; the compound pad is the glass fibrous membrane covered with the antigen-colloidal gold compound. The character is in that: the fibrous membrane includes three cover lines which cover the anti IgM antibody, the special antigen and the antibody according to the antigen separately. The invention detects the IgM antibody using the immune capture method principle and detects the IgG antibody by the double antigen sandwich method. So it can detect the special IgM, IgG antibody by one operation and the result has the high whole according ratio.

The invention provides a colloidal gold chromatography and preparation technology to detect specific IgM antibody. The chromatography uses colloidal gold as the marker, and uses immunohistochemistry capture method to detect and determine IgM antibodies, and in PVC backplanes, sequentially and mutually attached with sample pad, composite material pad, nitro cellulose membrane, absorbing pad; the nitro cellulose membrane includes the detection area and the control area, the characteristics being: the said composite material pad coated with antigen-colloidal gold composite glass cellulose membrane, and the said detection area covers the anti-IgM antibody, and the said control area covers the antibody corresponding to the antigen.

The invention discloses a kit and detection method for mycoplasma pneumoniae IgM on the basis of a fluorescence immunoassay chromatographic technique.The method comprises the steps that fluorescein serves as a marking material, and a mouse antihuman IgM mu chain monoclonal antibody is marked.A mycoplasma pneumoniae specific IgM antibody in serum is combined with a fluorescein marked antibody to form a reaction compound which is caught by a mycoplasma pneumoniae antigen, the mycoplasma pneumoniae IgM catch yield is in positive correlation with signal strength of the fluorescent antibody, and the mycoplasma pneumoniae IgM in the serum can be detected out by means of an immunofluorescence detector reading.The method has the advantages of being short in time, good in specificity, good in accuracy and stability and the like, and the coincidence rate of the detection result with that of a European Union mycoplasma pneumoniae IgM detection kit is high.A new method for rapidly and accurately detecting the mycoplasma pneumoniae IgM in clinic is provided, and a good market prospect is achieved.

The invention provides a reagent strip for joint detection of a syphilis specific IgM antibody and a specific total antibody and a preparation method thereof, relating to a reagent for joint detection of a syphilis specific IgM antibody and a specific total antibody. The reagent strip is provided with two reagent strips, wherein the two reagent strips are both provided with vector plates, sample adding pads, colloidal gold pads, nitrocellulose membranes, control lines and absorbent pads and respectively provided with a syphilis specific IgM antibody detection line and a specific total antibody detection line. The preparation method comprises the following steps: preparing recombinant syphilis antigens TPN17 and TPN47, applying samples of nitrocellulose membranes, preparing colloidal gold,labeling TPN17 and TPN47 with colloidal gold and preparing immunochromatography detection strips. The reagent strip can be used for detecting the syphilis specific IgM antibody and the specific totalantibody in the specimens of whole blood, blood serum, blood plasma, cerebrospinal fluid and the like. During detection, the specimen amount is minimal, special instruments are not needed and the results are directly interpreted by the naked eyes. The reagent strip is simple, convenient, fast, accurate and reliable and has strong specificity, high sensitivity, low cost and wide application range.

The invention aims at providing a simple and quick Zika virus detection kit. The kit optimally selects fusion expression protein as diagnostic antigen; an anti-human IgG monoclonal antibody A374, an anti-human IgM monoclonal antibody A371 and a biotin-BSA conjugate are respectively coated on a nitrocellulose membrane as a detection line and a quality control line; colloidal gold labeled fusion expression protein and colloidal gold labeled streptavidin and other reagents are matched; and an immunochromatography capture method principle is used for qualitative detection of Zika virus specific IgM antibody and IgG antibody in human serum, thereby realizing quick and specific diagnosis of Zika virus infection.

The invention relates to a SERS-immunochromatography detection method for rapidly and highly sensitively detecting Mycoplasma pneumoniae infection. The detection principle is that Nitrocellulose membranes (NC) are used as a carrier, a double-layer dye 5,5'-dithiobis (2-nitrobenzoic acid){5,5'-dithiobis- (2-nitrozoic acid), DTNB} labeled Au@Ag nano material coupling detection antibody is used as anSERS probe, a novel surface enhanced Raman spectroscopy (SERS-ICA)-based immunochromatography technology is established by combining the traditional Immunochromatography assay (ICA), and the technology is used for detecting human IgM and Mycoplasma pneumoniae (MP) specific IgM positive serum samples. The detection comprises the processes of sample dilution, SERS probe release, antigen-antibody reaction, result analysis and the like. The SERS-immunochromatography detection method for rapidly and highly sensitively detecting Mycoplasma pneumoniae infection can improve the detection sensitivityof human IgM and the detection rate of the lung branch positive serum specimen, and has important significance for clinical treatment.

The invention provides a test strip for rapid detection of a Brucella IgM antibody, which comprises a reaction film and a conjugate release pad. The reaction film has a detection band simultaneously coated with Brucella specific antigen bp26, and a quality control band coated with a double-antibody. The conjugate release pad is coated with colloidal golden labeled anti-human IgM monoclonal antibody. A membrane chromatography indirect sandwich method is adopted to detect the Brucella specific IgM antibody in a specimen. The test strip is simple in operation, convenient, and fast, and has the advantages of no requirements of special instruments and special training, clear and identified result, and easy popularization. The test strip is suitable for base course, site detection and early diagnosis, and has auxiliary effect on the diagnosis of Brucella infection.

The invention provides a western blot kit for cardiolipin and specific IgM antibodies of syphilis and preparation thereof, relating to a kit. The kit is provided with a carrier plate, a cellulose nitrate membrane, a detection line of the specific IgM antibodies and cardiolipin IgM antibodies of the syphilis and a control line, wherein the detection line and the control line are arranged on the cellulose nitrate membrane in sequence; specific recombinant antigens and cardiolipin antigens of the syphilis are coated at the detection line of the specific IgM antibodies of the syphilis, and human IgM antibodies are coated at the control line; and horse radish peroxidase is marked on anti-human mu-chain antibodies. The preparation comprises the following steps: preparing the specific recombinant antigens of the syphilis, then preparing the cardiolipin antigens, carrying out sample application on the cellulose nitrate membrane, and preparing mu-chain monoclonal antibodies of anti-human IgM specific segments; and marking the horse radish peroxidase on the anti-human IgM specific segments mu-chain monoclonal antibodies, and then preparing the western blot kit. The kit provided by the invention can be used for detection of the specific IgM antibodies and cardiolipin antigens of the syphilis in specimens such as whole blood, blood serum, blood plasma, cerebrospinal fluid and the like.

The invention discloses a method for detecting specificity of Epstein-Barr viruses (EBV) through an Epstein-Barr virus p18-p23 fused capsid antigen. The method is characterized in that an overlap extension polymerase chain reaction (PCR) technology is adopted; a coding gene p23 and a coding gene p18 are fused in vitro by a coding sequence of an intermediate head (Gly4Ser)3; a fused protein is expressed and the expressed fused protein is purified and is subjected to specificity identification through an immunoblotting technology; the purified fused protein is coated on an enzyme-linked immuno sorbent assay (ELISA) reaction plate and component content and reaction conditions are optimized; commercial horseradish peroxidase-labelled goat anti-human IgM and IgG and a series of other reagents are prepared; and EBV-viral cuspid antigen (VCA) specific IgM and IgG indirect ELISA diagnostic kit is obtained by assembling.

The invention provides a high-throughput treponema pallidum specific antibody detection kit and a preparation method thereof, and relates to treponema pallidum. The kit comprises an outer packaging box, an alkaline phosphatase labeled antihuman gamma monoclonal antibody bottle, an alkaline phosphatase labeled antihuman mu monoclonal antibody bottle, an alkaline phosphatase labeled antihuman Ig monoclonal antibody bottle, a chemiluminescent substrate bottle, a treponema pallidum specific antibody negative reference substance bottle, a treponema pallidum specific IgG antibody positive reference substance bottle, a treponema pallidum specific IgM positive reference substance bottle, a treponema pallidum specific total antibody positive reference substance bottle, a washing liquid bottle and a recombinant antigen coated microporous plate. The preparation method of the kit comprises the steps of firstly preparing a treponema pallidum specific recombinant antigen, the recombinant antigen coated microporous plate, an antihuman gamma chain monoclonal antibody, an antihuman mu chain monoclonal antibody and an antihuman Ig monoclonal antibody, labelling the alkaline phosphatases of the antibodies, and then preparing a chemiluminescent substrate, washing liquid and reference substances, and finally, assembling the high-throughput treponema pallidum specific antibody detection kit.

The invention provides a quick detection test strip for detecting leptospira IgM antibody, comprising a reaction film and a combination releasing pad; wherein the reaction film is provided with a detection zone capable of wrapping leptospira specific antigen ompL1 and LipL32 and a quality control zone encasing secondary antibody IgG, and the combination releasing pad is encased by anti-human IgM monoclonal antibody marked by colloidal gold. Leptospira specific IgM antibody in a specimen is detected by applying membrane chromatography capturing method. The test strip of the invention is applied to detection and is easy, convenient, fast and short-cut to operate, special instrument or equipment or professional training are not needed, the result is clear and easy to recognize, and the operation is easy, thus being easy to popularize, being applicable to basic level, field detection and early diagnosis, and being beneficial to leptospira infection diagnosis.

The invention provides a test strip for rapid detection of Japanese encephalitis virus IgM antibody. A Japanese encephalitis virus E gene antigen domain III and a double-antibody IgM III coat a nitrate cellulose film (NC film), and a membrane chromatography indirect sandwich method is adopted to detect the Japanese encephalitis virus specific IgM antibody in an infected human body specimen in combination with a colloidal gold labeled antihuman IgM monoclonal antibody. The test strip is simple in operation, convenient, and fast, and has the advantages of no requirements of special instruments and special training, clear and identified result, and easy popularization. The test strip is suitable for base course, site detection and epidemiological investigation, and has auxiliary effect on the diagnosis of Japanese encephalitis virus infection.

The invention discloses arg-gly-asp (RGD)-fused porcine circovirus 2 (PCV2) virus-like particles (VLPs), an infectious clone and a preparation method of the RGD-fused PCV2 VLPs, and application of theRGD-fused PCV2 VLPs and the infectious clone. According to the RGD-fused PCV2 VLPs, RGD oligopeptides are fused on PCV2 Cap proteins. According to the RGD-fused PCV2 VLPs, the PCV2 Cap proteins are subjected to RGD polypeptide sequence oligopeptide modification, the RGD oligopeptides are fused on the PCV2 Cap proteins, and the PCV2 VLPs with RGD-fused on the surfaces are assembled in vitro. Compared with wild PCV2 VLPs, the levels of PCV2 specific IgM and IgG antibodies generated by RGD-fused PCV2 VLP immune mice are significantly increased, high-titer PCV2 antibodies can be generated in an induced mode, and the RGD-fused PCV2 VLPs enhance humoral immunity response. The RGD-fused PCV2 VLPs can be applied to development of enhanced VLPs and differential diagnosis molecular label VLPs vaccines, and a new idea is provided for research and development of PCV2 vaccines.

The present invention provides a method for detecting hemorrhagic fever with renal syndrome (HFRS) antibody, belonging to the field of biological technology. It relates to a technique capable of utilizing immunomagnetic trapping technique and antigen protein of enzyme-labelled recombinant virus to detect antibody of HFRS due to Hantanvirus. Said method includes the following steps: purifying S gene recombinant protein of Hantanvirus representative strain Z10 and L99 expressed by pET prokaryotic expression system, labeling biotin or horseradish peroxidase, making magnetic microsphere be connected with sheep anti-human IgM (mu chain) or sheep anti-human IgG antibody and making it be sensitized to obtain immunomagnetic microsphere for detecting specific IgM/IgG antibody of seoul type or Hantan type of Hantanvirus and creating the quick and sensitive immunomagnetic microphere ELISA method for detecting HFRS specific IgM/IgG antibody in blood serum sample.

Provided herein are methods of sorting antigen-specific IgM memory B cells (MBCs), compositions and methods comprising such antigen-specific IgM MBCs, and recombinant antibody or antigen-binding fragments isolated from such antigen-specific IgM MBCs. As demonstrated herein, IgM and IgD MBCs are unique populations of cells with distinct phenotypic, functional and survival properties. Accordingly, the antigen-specific IgM MBCs and antibodies and antigen-binding fragments derived from these cells described herein are useful in therapeutic applications in vaccine strategies and treatment of infectious diseases.

The invention provides a combined detection card for simultaneously detecting specific IgM (Immunoglobulin M) and IgG (Immunoglobulin G) antibodies of ASFV (African Swine Fever Virus). An IgM detection strip and an IgG detection strip are combined on one detection card. Each detection strip comprises a sample pad, a sample combination pad, a chromatography membrane and a water absorption pad. The two chromatography membranes adopt the same recombinant ASFV antigens as solid phase coating substances, namely MGF 360-14L, CD2v and p72. The sample combination pad of the IgM detection strip is coated with biotin-labeled anti-pig IgM, a tracer-labeled streptavidin and a tracer-labeled rabbit anti-chicken IgY; and the sample combination pad of the IgG detection strip is coated with biotin-labeled anti-pig IgG, a tracer-labeled streptavidin and a tracer-labeled rabbit anti-chicken IgY. The invention also provides a preparation method and application of the combined detection card. The detection card is prepared by using an immunochromatography technology, the operation is convenient, and the result is visual.

The present invention relates to a new method for making recombinant antigen PP65 coated enzyme labeled reaction plate and ELISA kit. Its key technique mainly liesin that using gene engineering method to prepare specific recombinant antigen-HCMV pp65, after the antigen is exracted and purified, using the coating plate as important component in the kit, besides, after the anti-human IgM monoclone antibody prepared by utilizing mouse-mouse hybridome technique is labeled by using horseradish peroxidase, it can be used for assembly of kit. Said method capable of creating sensitive quick ELISA antibody to trap HCMV specific IgM antibody in serum has high specificity and high stability, and said kit mainly can be used in laboratory research and clinical diagnosis.

The invention discloses a babesia mocroti 2D97 antigen protein and application thereof. The protein has an amino acid sequence of SEQ ID NO:2 as shown in the specification, and has an encoded gene sequence of SEQ ID NO:1 as shown in the specification. An immunoreaction spectrum of babesia mocroti is obtained, results show that in a specific IgG antibody reaction, only one antigen protein 2D97 which has relatively intense immunoreactions with early-stage mouse serum of 3dpi and 7dpi is screened, and the antigen protein can be a candidate for early-stage antigen diagnosis. Escherichia coli prokaryotic expression is carried out with the nucleotide sequence of the antigen protein 2D97, a recombinant protein of escherichia coli is successfully obtained, and the protein has relatively intense immunoreactions with early-stage mouse serum of 3dpi and 7dpi in protein chip analysis of a specific IgM antibody, and can be applied to detection or diagnosis at an early stage and/or window stage within 3-7 days after infection of babesia mocroti.

The invention discloses a novel coronavirus COVID-2019 detection card and a preparation method thereof. The novel coronavirus COVID-2019 detection card comprises a card supporting layer attached to detection test paper, a sample unit positioned on the card supporting layer, a preprocessing unit positioned on the card supporting layer, a marker unit positioned on the card supporting layer, a detection unit positioned on the card supporting layer, and a recycling unit positioned on the card supporting layer; the sample units correspond to the sample holes; the pretreatment unit corresponds to the pretreatment hole; a hollow or transparent sealed detection window is arranged at the position, corresponding to the detection unit, of the shell; the erythrocyte agglutination layer is provided with erythrocyte agglutinin; the endogenous interfering substance treatment layer is provided with an endogenous interfering substance conjugate; the exogenous interfering substance treatment layer is provided with an exogenous interfering substance chelating agent; a non-specific IgM antibody treatment layer is provided with an IgM chelating agent; a non-specific IgG antibody treatment layer has anIgG precipitant. The problem that interfering substances influence the detection result of the novel coronavirus is solved, and the detection accuracy is improved.