Babesia voles 2d97 antigen protein and its application

A technology of antigenic protein and Babesia, applied in cell, proteomics and immunology research, and molecular fields, can solve the problems of unmonitored disease process, unclear antigen spectrum/immune response spectrum, and ineffective detection of samples
CN108893476BActive Publication Date: 2022-07-05中国疾病预防控制中心寄生虫病预防控制所国家热带病研究中心 +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
中国疾病预防控制中心寄生虫病预防控制所国家热带病研究中心
Publication Date
2022-07-05

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Abstract

The invention discloses a Babesia vole 2D97 antigen protein and application thereof. Its amino acid sequence is shown in SEQ ID NO: 2, and its coding gene sequence is shown in SEQ ID NO: 1. The present invention obtains the immune response spectrum of Babesia voles, and finds that in the specific IgM antibody response, only an antigen protein 2D97 which exhibits a higher immune response with the early 3dpi and 7dpi mouse serum is screened, which can be used as an early diagnosis. Antigen candidates. The nucleotide sequence of the antigenic protein 2D97 was used for prokaryotic expression in Escherichia coli, and its recombinant protein was successfully obtained. In the protein chip analysis of specific IgM antibody, it showed that it was higher than the early 3,7dpi mouse serum. The immune response can be used for detection or diagnosis in the early and / or window period of Babesia voles infection 3 to 7 days.
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Description

technical field

[0001] The invention relates to the fields of molecular, cellular, proteomic and immunological research, in particular to the preparation of Babesia voles immune proteins, the acquisition of immune response profiles, and the application in screening of candidate diagnostic antigens and immune protection vaccines. Background technique

[0002] Babesiosis is a zoonotic parasitic disease caused by the protozoa Babesia in red blood cells and is transmitted by tick bites and blood transfusions. There are more than 100 kinds of Babesia isolated from wild animals and livestock, among which the reported cases of human infection mainly include: B.microti and related pathogens, B.divergens and related pathogens, B.duncani and related pathogens, B. venatorum. In January 2011, Babesiosis was listed as a national notifiable infectious disease. In the United States, the majority of Babesia cases are caused by Babesia vole, and it has been reported that between 1979 and 2...

Claims

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