Method for detecting nephrotic syndrome hemorrhagic fever antibody

A technology for renal syndrome and hemorrhagic fever, which is applied in the field of specific IgM/IgG antibodies and pathogens of renal syndrome hemorrhagic fever, can solve the problems of false positives, large volume of enzyme plate, inconvenient portability, poor sensitivity, etc.
CN1811451AInactive Publication Date: 2006-08-02天津市卫生防病中心 +2
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
天津市卫生防病中心
Publication Date
2006-08-02
Estimated Expiration
Not applicable · inactive patent

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Abstract

The present invention provides a method for detecting hemorrhagic fever with renal syndrome (HFRS) antibody, belonging to the field of biological technology. It relates to a technique capable of utilizing immunomagnetic trapping technique and antigen protein of enzyme-labelled recombinant virus to detect antibody of HFRS due to Hantanvirus. Said method includes the following steps: purifying S gene recombinant protein of Hantanvirus representative strain Z10 and L99 expressed by pET prokaryotic expression system, labeling biotin or horseradish peroxidase, making magnetic microsphere be connected with sheep anti-human IgM (mu chain) or sheep anti-human IgG antibody and making it be sensitized to obtain immunomagnetic microsphere for detecting specific IgM / IgG antibody of seoul type or Hantan type of Hantanvirus and creating the quick and sensitive immunomagnetic microphere ELISA method for detecting HFRS specific IgM / IgG antibody in blood serum sample.
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Description

Technical field:

[0001] The present invention relates to the preparation and labeling of Hantavirus (HV) S gene recombinant protein and the preparation of immunomagnetic microspheres, the pathogen of hemorrhagic fever with renal syndrome (HFRS). Immunomagnetic microsphere-ELISA method to detect specific IgM / IgG antibodies in serum samples of patients with hemorrhagic fever with renal syndrome. Belongs to the field of biotechnology. technical background:

[0002] my country is the country most seriously affected by hemorrhagic fever with renal syndrome. The number of cases each year accounts for more than 90% of the total reported in the world, and the fatality rate is extremely high. Attention at home and abroad. Most of the studies on the diagnosis and prevention of the disease are carried out by Chinese scholars. The disease has an acute onset, complicated clinical manifestations, prominent kidney damage, and no specific drug, which is seriously harmful. Therefore, early...

Claims

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