18results about How to "Reduce research costs" patented technology

The application discloses a method for predicting the flow velocity along the course of a river with floating vegetation, and the river with floating vegetation is divided along the water flow direction of the river according to the flow regulation distance, and the water flow direction velocity prediction model of the river with floating vegetation is constructed to realize the prediction of the flow velocity along the course of the river, so as to provide a theoretical basis for further research on the water flow, sediment movement characteristics and riverbed evolution of the natural river and lake with floating vegetation.

The invention provides a red-blue confrontation-based large model agent autonomous iterative optimization method and system, and the method comprises the steps: building a customer agent as a blue party based on a large language model, obtaining user data from a plurality of channels, generating diversified customer portrait data, generating a dialogue test set containing diversified customer consultation test data, and carrying out the calculation of the dialogue test set. Taking the data as adversarial data for iterative optimization of the model; the method comprises the following steps: constructing a sales agent as a red party by using a preset first version prompt word, starting the red party and a blue party to carry out dialogue based on adversarial data, limiting a maximum dialogue round or taking a transaction conclusion as an exit condition, and carrying out quality inspection scoring and prompt word optimization direction prompting on the sales agent through a plurality of evaluation dimensions; inputting the first version cue word and the cue word optimization direction to a sales agent to obtain an optimized sales agent cue word; and repeating until the quality inspection score converges, and completing the iterative optimization process of the sales agent. According to the method, the sales conversion rate is remarkably improved, and the manual optimization cost is greatly reduced.

This utility model relates to the field of soil research technology and discloses an equidistant sampling device suitable for the study of soil pollutants in agricultural land. It includes a support plate with a scale fixedly connected to its top. Two I-shaped grooves are formed at the top of the support plate, and two I-shaped sliders are slidably connected to the inner walls of the two grooves. Two pedals are fixedly connected to the tops of the two sliders. The operator first determines the sampling interval according to the research requirements. Then, the operating parameters of the bidirectional motor, such as speed and direction of rotation, are set via the control panel. After starting the bidirectional motor, its output drives a bidirectional screw to rotate. Due to the bidirectional thread characteristic of the bidirectional screw, the two moving sleeves connected to the surface of the bidirectional screw will move in opposite directions at equidistant intervals along the screw. The operator can then observe the position of the pointer on the scale.

The invention relates to the technical field of coal rock, in particular to a fluid action induced coal rock strain method based on dynamic ensemble simulation, which comprises the following steps: constructing a coal rock macromolecular model; determining the type of fluid to be researched; simulating the coal rock macromolecular models of different fluid types by utilizing a dynamic ensemble; and constructing a coal rock strain model induced by different fluid effects. The method solves the problems that existing molecular dynamics simulation lacks reasonable selection and application of a dynamics ensemble, and the strain condition of coal rock under the actual working condition is difficult to accurately simulate.

The present application relates to the technical field of artificial intelligence and black light experiment collaborative control, in particular to a black light experiment collaborative control system and method based on multi-agent collaborative decision. It includes multi-agent collaborative decision module, digital twin and protocol conversion module, black light experiment execution module, human-computer interaction quality control module and result analysis and skill sedimentation module. Through the cooperation of the five modules of multi-agent collaborative decision, digital twin and protocol conversion, multi-actuator arrangement, human-computer interaction quality control, result analysis and skill sedimentation, end-to-end full closed loop from natural language scientific research task input to 7*24 hours unattended experiment execution, full-dimensional data quality control to structured skill sedimentation reuse is realized. The pain points of high dependence on traditional scientific research manpower, poor experimental reproducibility and difficult knowledge inheritance are solved, and the scientific research efficiency and achievement quality are greatly improved.

The invention belongs to the technical field of structural design of test devices, and discloses a servo mechanism combined vibration device which comprises a support I, a support II, a support III, a supporting frame, a reinforcing column, a reinforcing plate, a supporting cylinder, a base I, a base II and the like. The reinforcing column is connected with a support I and a support II, and independent transmission mechanisms can be installed on the support I and the support II. The support II and the support III are connected through a reinforcing plate, and a control assembly can be installed on the reinforcing plate. A supporting frame is installed between the support II and the support III, and the transmission mechanism and the control assembly can be integrated together through the supporting frame, a reinforcing column and a reinforcing plate and can also be installed separately and independently. The supporting cylinders at the two ends of the tool are used for meeting different clamp connector requirements. The split-type servo mechanism can be integrated into a cabin section by simulating a projectile body structure form, so that the disassembly and assembly frequency in the production circulation process is reduced.

The present application relates to a kind of soybean polypeptide preparation method with antioxidant and antagonistic lead damage effect.The soybean polypeptide prepared in the present application has good DPPH free radical clearance rate, ABTS free radical clearance rate and Fe3+ reduction capacity in vitro;It can also alleviate the case that the body weight of mouse is slowly increased due to lead exposure, improve the learning and memory ability of lead exposure mouse, alleviate the organ damage of mouse caused by lead exposure, enhance the level of reduced glutathione, superoxide dismutase and catalase of organism, and alleviate the abnormal increase of malondialdehyde caused by lead exposure.The soybean polypeptide prepared in the present application has good in vitro antioxidant capacity, and has antagonistic effect on oxidative stress caused by lead exposure mouse, and can be used as non-toxic and harmless natural antioxidant.For the development of natural antioxidant food, it has far-reaching application prospect, and also provides a new feasible idea for the combined application of bioinformatics technology and traditional enzymatic technology.

The invention discloses a high-voltage electrified body-hot-line worker gap corona development model and a pilot start judgment method, and the method comprises the steps: carrying out the judgment according to the discharge physical process of a hot-line work combination gap, based on a long air gap discharge corona development model, a pilot start criterion and the like; a high-voltage electrified body-electrified worker gap corona development model is established, and a pilot initial judgment method corresponding to the model is provided. The model can simulate the corona development physical process of the gap between the high-voltage electrified body and the live working personnel under the operation impulse voltage by respectively simulating the potentials of the high-voltage electrified body and the live working personnel in the discharge development process, so as to calculate the regional space charge quantity of the gap corona region and judge whether the pilot in the gap is started or not; and a new model and a new method can be provided for live-line work combined gap discharge simulation research.

The utility model discloses a novel agar salt bridge type electrical stimulation cell culture device which comprises a base, a top cover, a salt bridge positioning module and an electrical stimulation module, the top cover is mounted on the base, and a plurality of groups of optical observation windows are arranged on the top cover; each group of salt bridge positioning modules comprises a base and a salt bridge positioning buckle; an adjusting mechanism is arranged between the salt bridge positioning buckle and the base, and the adjusting mechanism is used for adjusting the height of the salt bridge positioning buckle; the electrical stimulation module comprises a PCB (Printed Circuit Board) and a plurality of groups of electrode columns; the PCB is mounted on the top cover; and the electrode column is arranged on the PCB through a threaded connection structure. The novel agar salt bridge type electrical stimulation cell culture device disclosed by the utility model can be matched with a standard commercial cell culture dish for use, the universality is wider, an experimenter can conveniently observe the cell / tissue state in the commercial cell culture dish, and meanwhile, the electric field intensity gradient can be conveniently adjusted.

This invention belongs to the field of biomedical technology and discloses an in vitro evaluation method for GBM drug delivery systems based on tumor organoid chips. This method uses a PDMS tumor organoid chip as a carrier, co-culturing GBM cells and human umbilical vein endothelial cells to localize and culture GBM cells within the chip chamber. A biomimetic blood-brain barrier is constructed within the microchannels, and a perfusion system continuously maintains the cell growth microenvironment. After in vitro drug administration to the test drug delivery system, its anti-GBM effect is quantitatively detected from three dimensions: proliferation, invasion, and spheroidization. This invention can highly simulate the in vivo GBM tumor microenvironment and blood-brain barrier structure, achieving precise, real-time, and dynamic in vitro efficacy evaluation, while significantly reducing reliance on animal experiments, improving the reliability of results and experimental throughput. It provides an efficient and standardized platform for preclinical screening and mechanism of action research of anti-GBM drug delivery systems, and has significant scientific research value and clinical translational significance.

The present application relates to a kind of bionic lymph node organoids and its construction method, the organoids include in three-dimensional space: one B cell-rich core region;And one T cell-rich region, it is at least partially wrapped in the core region in space;Wherein, the core region can be differentiated to form the light area and dark area with structural difference in response to external stimulation.The method includes preparing first bioink and second bioink, the first bioink contains B lymphocyte, and the second bioink contains T lymphocyte;Using the first and second bioink, the organoid prototype with the spatial structure of T cell-rich region wrapping B cell-rich region is formed by three-dimensional construction technology;The organoid prototype is solidified to stabilize its structure;External stimulation is applied to the structure after solidification, and the B cell-rich region is differentiated to form light area and dark area.

This invention relates to the field of simulation and computation technology, specifically to a simulation analysis method and apparatus for aircraft in-flight water drop based on VOF-DPM. It addresses the problems of actual flight tests being significantly affected by weather, incurring high manpower and material costs, and difficulties in measuring local dynamic physical quantities, as well as the time-consuming nature and scale-effect errors in scaled-down model tests. The method includes setting up a flow field domain, dividing it into an air domain and a water domain; spatially discretizing the flow field domain based on a Cartesian octree grid; effectively capturing the free interface of the gas-liquid two-phase flow using a VOF-DPM combined h-type refined adaptive grid; and progressively refining the local grid of the Cartesian octree grid, with a grid refinement standard of 2 in each direction. x The physical parameters of the flow field are initialized; the position and shape of the phase interface are captured using the VOF method based on grid resolution; and the free-moving droplets are simulated to realize the simulation analysis of airborne water droplets from aircraft.

The invention provides an electric pulse processing device and method, and belongs to the technical field of material processing, the electric pulse processing device comprises a base, two clamping pieces, a power supply, a pulse generator and a resistance regulator; two first fixing parts and a second fixing part are arranged at the upper end of the base, the base is made of an insulating material, and a sample is mounted on the second fixing part; the clamping piece is fixedly connected to the two first fixing parts; the clamping piece is a piece made of a conductive material, and two clamping ends of the clamping piece are respectively clamped on a sample; the power supply is connected with the two clamping pieces and forms a conductive path with the sample; the pulse generator is connected to the conductive path; a control switch connected to the conductive path is arranged on the pulse generator; the resistance regulator is connected to the conductive path and used for regulating the current of the conductive path. According to the electric pulse treatment device, the performance improvement effect of the metal material can be improved, and an electron microscope can be easily used for micromechanism research.

The invention relates to the technical field of computer simulation, and provides a simulation model construction method for stimulating a deep brain area by electromagnetic waves, which comprises the following steps: constructing a brain finite element model, a semi-ellipsoid reflection structure and an electromagnetic wave emission source based on a COMSOL simulation software radio frequency module; the semi-ellipsoid reflection structure is located in the external space of the brain and comprises two semi-ellipsoid reflection surfaces with the same major semi-axis and the same minor semi-axis. The second focuses of the two semi-ellipsoid reflecting surfaces coincide in a deep brain region; the electromagnetic wave emission source comprises a lumped power supply port, a first patch antenna and a second patch antenna, wherein the first patch antenna and the second patch antenna are located at first focuses of the two semi-ellipsoid reflecting surfaces respectively. The first patch antenna and the second patch antenna respectively emit two beams of high-frequency carrier electromagnetic waves, and a preset tiny frequency difference exists between the frequencies of the two beams of high-frequency carrier electromagnetic waves. Through the method, a superficial brain region is bypassed, simulation modeling is performed on a deep brain region non-invasive electrical stimulation scheme, and model parameters are optimized.

This invention discloses a method for analyzing the mobility of original formation water in gas reservoirs, comprising the following steps: S1, acquiring target well and target layer image data, and constructing a three-dimensional multi-pore structure model; S2, restoring the original formation conditions to the three-dimensional multi-pore structure model; S3, establishing a gas-water two-phase displacement mathematical model based on the lattice Boltzmann method, and coupling the two to obtain a coupled model; S4, determining the original state of the gas reservoir, setting evolutionary solution conditions, and performing evolutionary calculations on the coupled model based on the evolution equation to obtain a three-dimensional multi-pore structure model containing both gas and water phases; S5, performing multi-angle slice analysis, classifying and analyzing the mobility of the water phase, and obtaining the distribution of mobile water. This method does not require closed coring operations and avoids subsequent high-cost experiments such as nuclear magnetic resonance and core displacement, effectively reducing research costs, providing accurate and reliable prediction results, greatly shortening the testing and analysis cycle, and realizing the evaluation of formation water mobility under the original conditions of gas reservoirs.

The application relates to the technical field of cell culture, and discloses a construction method of a laying hen liver organoid natural lipid deposition model, which verifies that the functions related to liver tissue lipid synthesis and transport of 1-day-old laying hens are more active, the separated liver organoids and liver primary cells present the same trend, and there is more lipid deposition, so the organoids cultured by the primary liver cells of 1-day-old laying hens are selected as a kind of natural in-vitro lipid deposition model. In order to solve the technical problem that it is difficult to establish an endogenous lipid deposition model of laying hens, the application improves the organoid inoculation and culture technology by separating the primary liver cells of 1-day-old laying hens, successfully constructs a kind of primary liver cell-derived laying hen liver organoid natural lipid deposition model, reduces the research cost, and has higher lipid metabolism activity, and provides an ideal ex vivo model for the research on laying hen liver lipid metabolism and related disease mechanisms.

This invention provides a 3'-UTR element and its application in the production of target proteins. The 3'-UTR element comprises: (a) a nucleic acid molecule with a nucleotide sequence as shown in any of SEQ ID NO. 23, SEQ ID NO. 1-SEQ ID NO. 22, SEQ ID NO. 24-SEQ ID NO. 28, and SEQ ID NO. 37; (b) a nucleic acid molecule transcribed using the antisense strand of (a) as a template; or (c) a nucleic acid molecule derived from (a) or (b) by substitution, deletion, or addition of one or more nucleotides in the nucleotide sequence defined by (a) or (b) while retaining the biological function of its derived sequence. This element can stably and significantly enhance mRNA gene expression, providing a powerful tool for the development of mRNA vaccines and the optimization of gene expression in gene therapy.

The application discloses a method for inducing the mycelial phase of Leucomycicella bicuspidata based on carbon quantum dots and application thereof, and the method comprises the following steps: preparing carbon quantum dots by a hydrothermal method, inducing culture of the mycelial phase, and morphological observation and confirmation. The carbon quantum dots are prepared by hydrothermal reaction of citric acid and urea at 230 DEG C for 15 hours, and the particle size is 2-8 nm; the carbon quantum dots are added into a Sabouraud glucose culture medium, the final concentration is 0.5 mg / mL, and the Leucomycicella bicuspidata is cultured at 28 DEG C for 24-28 hours, so that the mycelial phase of the Leucomycicella bicuspidata can be significantly induced; in combination with microscopic observation and PCR molecular confirmation, the mycelial formation rate can reach more than 85%. The method provides stable and reliable experimental materials for the study of the pathogenic mechanism and the prevention and control technology of the Leucomycicella bicuspidata.