Method for carrying out test tube grafting and rejuvenization on paulownia fortunei select tree

A white-flowered paulownia and test-tube technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of low asexual reproduction ability and serious maturation effect, improve the success rate and efficiency of juvenile, and reduce scientific research. Cost and effect of shortening the nurturing cycle

Inactive Publication Date: 2011-09-07
CHINA PAULOWNIA RES CENT
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AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to develop a kind of paulownia white flower excellent tree test tube grafting juvenile for the serious maturation effect of the excellent tree material of Paulownia white flower and low asexual reproduction ability. method

Method used

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Embodiment 1

[0025] Embodiment 1: Take the paulownia paulownia tree 'Baiyou 3' as an example to further describe the present invention in detail.

[0026] A method for test-tube grafting and hatching of paulownia white flower paulownia excellent tree, the method comprises the following steps:

[0027] (1) Grafting and rejuvenation of materials: firstly, the branches of 'Baiyou 3', collected in autumn and early spring and treated with indoor sand storage in winter, were grafted in mid-April. The robust paulownia chuanyou tree 'Chuanyou 3' clone of 5 cm in length is used as the rootstock. After the scion germinates, remove the buds on the rootstock in time, and strengthen the management of water, fertilizer and pests according to the growth situation;

[0028] (2) Tissue culture and in vitro grafting: in mid-May, shoots of grafted seedlings with a length of more than 20 cm are collected for tissue culture and in vitro grafting. The process includes the following steps:

[0029] The first st...

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Abstract

The invention provides a method for carrying out test tube grafting and rejuvenization on paulownia fortunei select tree, comprising the following steps: grafting and rejuvenation of select trees; tissue culture and test tube grafting and rejuvenization; explant sterilization; primary culture; test tube grafting and rejuvenization; secondary enrichment culture; induced rootedness; and seedling adaptation and transplantation. In the invention, the select trees suffering from serious ripening are grafted and rejuvenated; then tissue culture and test tube grafting and rejuvenization are carried out on the select tree to overcome the defects that the root segment of the paulownia fortunei select tree is difficult and the germination rate of buffing roots is low so as to effectively realize rejuvenization of the paulownia fortunei select tree, shorten rejuvenization cycle of the paulownia fortunei select tree, and powerfully promote breeding process of the paulownia fortunei. In the invention, the pronounced problem that the paulownia fortunei select tree suffers from serious ripening and low capacity in vegetative propagation can be solved, rejuvenization success rate and rejuvenization efficiency of the paulownia fortunei select tree can be improved and scientific research costs can be reduced.

Description

Technical field: [0001] The invention relates to a method for juvenile paulownia excellent trees, in particular to a method for in vitro grafting and young paulownia white flower excellent trees, which is suitable for young paulownia white flower excellent tree materials with serious maturation effect, and improves the growth rate of paulownia white flower Asexual reproduction ability of Youshu. Background technique: [0002] Paulownia is one of the most important fast-growing high-quality timber tree species and ecological protection tree species in my country. It has always played an irreplaceable role in the construction of commercial forests and ecological environment protection in China. my country is also the country with the richest germplasm resources and the widest distribution range of paulownia in the world. In the mainland alone, there are nine species with two varieties and many variant types. Among them, Paulownia albiflora grows fast, has a wide range of adap...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G1/06
Inventor 李芳东邓建军傅建敏刘昌勇张悦梁玉琴吴硕黄琳
Owner CHINA PAULOWNIA RES CENT
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