This invention discloses PCR primers and identification methods for distinguishing the sex of
poplar trees from five major groups and the sex
genotype of the
white poplar group. This invention is based on the sex-determining
gene in
poplar trees. FERR Genes and FERR-R
Specific primers were designed for each
gene, and a PCR primer set for detecting the sex of
poplar trees from five different groups was finally obtained through screening. PCR detection results of poplar materials with known sex using this primer set were completely consistent with the actual poplar sex
phenotype, demonstrating that the PCR primer set provided by this invention can accurately identify the sex of poplar trees from the five different groups at the
molecular level, making poplar sex identification more universal. Furthermore, the specific amplification provided by this invention... FERR The primer pairs for the
gene can accurately identify the sex
chromosome composition of poplar species. This invention has significant application prospects in the identification of male and female sexes in the five major poplar species,
molecular breeding of poplars, and the control of female tree fluff.