Rejuvenating method for tissue culture of superior nanmu trees

A tissue culture and nanmu technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of serious maturation effect, low asexual reproduction ability, etc., to improve the success rate, improve the efficiency of juvenile and reduce scientific research. cost effect

Inactive Publication Date: 2019-02-05
HUBEI UNIV FOR NATITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to study a kind of method for the tissue culture and juvenileization of excellent nanmu trees, aiming at the serious maturation effect of nanmu excellent tree materials and low asexual reproduction ability

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1: Taking the excellent tree "Nanyou 5" as an example, the present invention will be further described in detail.

[0029] A method for tissue culture larvae of Nanmu excellent tree, the method may further comprise the steps:

[0030] (1) Grafting and rejuvenation of materials: Firstly, the branches of "Nanyou 5" excellent trees collected in autumn and early spring were grafted in late March. Phoebe is used as the rootstock. After the scion germinates, the buds on the rootstock should be wiped off in time, and the management of water, fertilizer and pests should be strengthened according to the growth situation;

[0031] (2) Tissue culture and test tube grafting young: in the first ten days of May, the twigs of grafted seedlings with a length of more than 10cm are collected for tissue culture and test tube grafting. The process includes the following steps:

[0032] The first step, explant sterilization: collect grafted and rejuvenated Phoebe excellent tree ...

Embodiment 2

[0045] This example is basically the same as Example 1, except that, when sterilizing the explant surface, the alcohol concentration is 85%, and the soaking time is 3-5 seconds.

Embodiment 3

[0047] This example is basically the same as Example 1, except that when the primary culture is carried out, the culture condition room temperature is 28-29°C, the light intensity is 2000-3000 lux, and the light time is 6:00 every day ~22:00.

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Abstract

The invention belongs to the technical field of cultivation of nanmu seedlings, and relates to a rejuvenating method for superior nanmu trees. The method includes the steps of grafting and rejuvenating of superior nanmu tree materials, explant sterilization, primary culture, test tube grafting and rejuvenating, subculture multiplication culture, root induction, seedling hardening and transplanting. The rejuvenating method for tissue culture of the superior nanmu trees has the advantages that the superior nanmu trees have high twig cuttage survival rate and strong asexual reproduction ability,and the like; the problem of difficulty in collection of seeds, branches and roots of the superior nanmu trees is solved, the rejuvenation cycle of the superior nanmu tree materials is shortened, andthe rejuvenation efficiency is improved greatly; therefore, a new way is provided for rejuvenation of superior nanmu trees, and the nanmu breeding process is promoted vigorously.

Description

technical field [0001] The invention relates to a method for tissue culture juvenile of excellent nanmu trees, which is to sterilize the excellent tree materials with serious maturation effect of nanmu, thereby improving the asexual reproduction ability of the excellent nanmu trees. Background technique [0002] Phoebe is a subtropical evergreen broad-leaved tall tree. It is a rare and endangered tree species unique to my country. The wood material is excellent, hard and rot-resistant. In my country, Phoebe is mainly distributed in the west of Hubei Province, the northwest of Guizhou Province, Sichuan Province and Chongqing City. Its tree shape is tall and straight, its branches and leaves are luxuriant, and it is evergreen in all seasons. It is the main tree species that make up evergreen broad-leaved forests. Excellent adult tree. However, due to various reasons such as natural and man-made, the germplasm resources of Phoebe in our country are decreasing day by day, which ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G2/30
CPCA01H4/001A01H4/008A01G2/30
Inventor 崔令军石开明林健
Owner HUBEI UNIV FOR NATITIES
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