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Tissue culture and rapid propagation method for paulownia catalpifolia superior tree

A technology for rapid propagation of paulownia catalpa and tissue culture, applied in horticultural methods, botany equipment and methods, plant regeneration, etc., can solve problems such as difficulty, reduction, and difficult differentiation of cuttage propagation callus, and achieve the solution of difficult differentiation, Solve the serious effect of maturation and realize the effect of rapid asexual reproduction

Inactive Publication Date: 2020-04-21
中国林业科学研究院经济林研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a kind of tissue culture and rapid propagation of Paulownia catalpa in order to solve the outstanding problems that the fine germplasm resources of Paulownia catalpa are decreasing day by day, the roots are senile and degraded seriously, and the collection of root segments is difficult, and the callus of cutting propagation is difficult to differentiate. method

Method used

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  • Tissue culture and rapid propagation method for paulownia catalpifolia superior tree
  • Tissue culture and rapid propagation method for paulownia catalpifolia superior tree
  • Tissue culture and rapid propagation method for paulownia catalpifolia superior tree

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Embodiment 1

[0034] A method for tissue culture and rapid propagation of an excellent paulownia catalpa tree, comprising the steps of grafting and rejuvenation, selection of explants, treatment of explants, primary culture, subculture, rooting culture, seedling hardening and transplanting.

[0035] The operation steps are as follows:

[0036] (1) Grafting and rejuvenation of materials: Before the spring germination in the deciduous period, the annual branches of No. 1 Paulownia catalpa leaf tree were collected, and grafted on March 25. The grafting method was split grafting, and the ground diameter was selected to be 3 cm to 5 cm. The robust 9501 clone is used as the rootstock. After the scion germinates, the buds on the rootstock should be wiped out in time, and the management of water, fertilizer and pests should be strengthened according to the growth conditions;

[0037] (2) Selection of explants: Collect the young shoots of the grafted seedlings of Paulownia catalpa in the growing sea...

Embodiment 2

[0044] A method for tissue culture and rapid propagation of an excellent paulownia catalpa tree, comprising the steps of grafting and rejuvenation, selection of explants, treatment of explants, primary culture, subculture, rooting culture, seedling hardening and transplanting.

[0045] The operation steps are as follows:

[0046] (2) Grafting and rejuvenation of the material: before germination in spring in the deciduous period, the annual branches of No. 1 paulownia catalpa tree were collected and grafted on March 6. The grafting method was split grafting, and the ground diameter was selected to be 3 cm to 5 cm The robust 9501 clone is used as the rootstock. After the scion germinates, the buds on the rootstock should be wiped out in time, and the management of water, fertilizer and pests should be strengthened according to the growth conditions;

[0047] (2) Selection of explants: Collect the young shoots of the grafted seedlings of Paulownia catalpa in the growing season fo...

Embodiment 3

[0054] A method for tissue culture and rapid propagation of an excellent paulownia catalpa tree, comprising the steps of grafting and rejuvenation, selection of explants, treatment of explants, primary culture, subculture, rooting culture, seedling hardening and transplanting.

[0055] The operation steps are as follows:

[0056] (3) Grafting and rejuvenation of materials: Before the spring germination in the deciduous period, the annual branches of No. 1 paulownia catalpa tree were collected and grafted on April 15. The grafting method was split grafting, and the ground diameter was selected to be 3 cm to 5 cm. The robust 9501 clone is used as the rootstock. After the scion germinates, the buds on the rootstock should be wiped out in time, and the management of water, fertilizer and pests should be strengthened according to the growth conditions;

[0057] (2) Selection of explants: Collect the young shoots of the grafted seedlings of Paulownia catalpa in the growing season fo...

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Abstract

The invention relates to a tissue culture and rapid propagation method for a paulownia catalpifolia superior tree. In a growth season, young branches of paulownia catalpifolia grafted seedlings are collected and disinfected, and then tissue culture is performed. The method comprises the following steps of: (1) grafting and rejuvenation of materials, (2) explant selection, (3) explant treatment, (4) primary culture, (5) subculture, (6) rooting culture, and (7) seedling acclimatization and transplantation. By the method, the key problems that paulownia catalpifolia callus is difficult to differentiate, and propagation materials are not easy to obtain are solved, rapid vegetative propagation of the paulownia catalpifolia superior tree materials is successfully achieved through a tissue culture technology for the first time, and a technical guarantee is provided for later storage of paulownia catalpifolia resources and rapid propagation of a large number of seedlings needed for breeding ofexcellent clones.

Description

technical field [0001] The invention relates to a method for tissue culture rapid propagation of an excellent Paulownia catarrhalis tree, which is suitable for the rapid propagation of an excellent Paulownia catarrhalis tree whose callus is difficult to differentiate and whose propagation materials are difficult to obtain, and can improve the asexual reproduction ability of the Paulownia catarrhalis. Background technique [0002] Paulownia is one of the important fast-growing timber tree species in China, and occupies a pivotal position in the commercial timber market of construction, furniture, decorative materials, wood-based panels, musical instruments and so on in my country and even in the world. At present, my country has 11 species, 2 varieties and many types of variation such as inter-species and intra-species. Among them, Paulownia catalpa has high wood density, excellent material color, beautiful pattern, strong natural drying ability and narrow crown width. It is ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G2/30
CPCA01H4/008A01G2/30
Inventor 乔杰王保平冯延芝赵阳张悦周海江段伟杨超伟
Owner 中国林业科学研究院经济林研究所
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