The invention discloses a formula and a production method for preparing a deep yellow wheat straw dye. Gamboge tree resin, grass bouquet box, amur corktree, madder, senna leaf, alumen, a ferric ion compound solution and spring water are taken as main raw materials. The production method comprises the following main technological processes: sorting, cropping, drying, soaking, hot boiling, mixing, participating, dyeing, and forming a product. According to the formula and the production method for preparing the deep yellow wheat straw dye, the deep yellow wheat straw dye prepared from pure natural plants is pure natural, free of pollution, green and environment-friendly, and free of toxic and side effects on a human body.