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Method for improving soybean herbicide dicamba resistance and plant light sensitivity through transgenic DMO (Dicamba Monooxygenas) gene

A technology of transgenic plants and DNA molecules, applied in botany equipment and methods, biochemical equipment and methods, plant products, etc., can solve few problems

Active Publication Date: 2019-03-08
NANJING AGRICULTURAL UNIVERSITY +2
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

However, there are relatively few reports on the application of the dicamba-degrading monooxygenase (DMO) gene in soybeans in China.

Method used

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  • Method for improving soybean herbicide dicamba resistance and plant light sensitivity through transgenic DMO (Dicamba Monooxygenas) gene
  • Method for improving soybean herbicide dicamba resistance and plant light sensitivity through transgenic DMO (Dicamba Monooxygenas) gene
  • Method for improving soybean herbicide dicamba resistance and plant light sensitivity through transgenic DMO (Dicamba Monooxygenas) gene

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Embodiment Construction

[0045] The technical solution route of the present invention is as follows: construct a plant expression vector containing the DMO gene; introduce the DMO gene into soybeans through the cotyledonary node transformation method mediated by Agrobacterium, obtain resistant plants through dicamba screening, and perform genomic DNA level analysis on resistant rooted plants. PCR and protein-level test strip detection to verify whether the exogenous gene is integrated into the genome of the transgenic plant and transcribed. The resistance analysis of transgenic lines confirmed the function of DMO gene in soybean herbicide resistance. Technical means of the present invention specifically comprises the following steps:

[0046] 1. Codon optimization of the target gene and construction of plant expression vectors and genetically engineered bacteria

[0047] Referring to the full-length gene sequence CDS (GenBank no.AAV53699) of DdmC from Pseudomonas maltophilia strain DI-6, express the ...

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Abstract

The invention discloses a method for improving soybean herbicide dicamba resistance and plant light sensitivity through a transgenic DMO (Dicamba Monooxygenas) gene. A DNA (Deoxyribonucleic Acid) molecule which has a nucleotide sequence as shown in SEQ ID NO.2 is obtained through codon optimization on a DMO gene, and herbicide-resistant transgenic soybeans are cultured by using the gene. The invention provides a solving method for weed growth in agriculture, meanwhile, the labor expenditure for manual weeding is reduced, and convenience in production and utilization is achieved. As a vector contains a broad spectrum herbicide resistant gene, the weeding range of a plant can be further widened, and application ranges can be widened. Meanwhile, leaves of the transgenic DMO gene soybeans grownormally under weak light, and the soybeans have utilization potential under shielding conditions such as intercropping.

Description

technical field [0001] The invention belongs to the fields of molecular biology, genetic engineering technology and transgenic breeding, relates to a dicamba monooxygenase gene (Dicamba monooxygenas, DMO) and its application in the cultivation of herbicide-tolerant transgenic soybeans, and provides a method for cultivating wheatgrass resistant to wheatgrass by using the gene A method for transgenic soybeans resistant to fear, a method for detecting and identifying DMO overexpression vectors, engineering bacteria, and positive transgenic seedlings used in the cultivation process. Background technique [0002] Weeds can compete with crops for growth space and nutrients, and at the same time spread various diseases and insect pests, causing serious damage to crop growth and development (Wang Yilin, Li Hong. Research status of herbicide-resistant transgenic plants[J]. Biology Bulletin, 2005, 40(4):15-17; Li Yunhe, Li Xiangju, Peng Yufa. Global Development and Utilization of Trans...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/53C12N15/82A01H5/00A01H6/54
CPCC12N9/0004C12N15/8274
Inventor 赵团结林忠平张莹莹邱丽娟孔杰杰
Owner NANJING AGRICULTURAL UNIVERSITY
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