Method for improving soybean herbicide dicamba resistance and plant light sensitivity through transgenic DMO (Dicamba Monooxygenas) gene
A technology of transgenic plants and DNA molecules, applied in botany equipment and methods, biochemical equipment and methods, plant products, etc., can solve few problems
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[0045] The technical solution route of the present invention is as follows: construct a plant expression vector containing the DMO gene; introduce the DMO gene into soybeans through the cotyledonary node transformation method mediated by Agrobacterium, obtain resistant plants through dicamba screening, and perform genomic DNA level analysis on resistant rooted plants. PCR and protein-level test strip detection to verify whether the exogenous gene is integrated into the genome of the transgenic plant and transcribed. The resistance analysis of transgenic lines confirmed the function of DMO gene in soybean herbicide resistance. Technical means of the present invention specifically comprises the following steps:
[0046] 1. Codon optimization of the target gene and construction of plant expression vectors and genetically engineered bacteria
[0047] Referring to the full-length gene sequence CDS (GenBank no.AAV53699) of DdmC from Pseudomonas maltophilia strain DI-6, express the ...
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