High-throughput treponema pallidum specific antibody detection kit and preparation method thereof

The technology of Treponema pallidum and detection kit is applied in the directions of measuring devices, instruments, scientific instruments, etc., which can solve the problems of low sensitivity, low detection positive rate and high false positive rate of cardiolipin antibody, and achieves accurate results, simple operation, The effect of sensitive detection

Active Publication Date: 2015-02-04
ZHONGSHAN HOSPITAL XIAMEN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology allows us to accurately identify different types of immunoglobulins called trepilin that help fight against malaria bacteria (TSB). These proteins include both free or bound forms of these molecules found naturally within red blood cells from humans with symptoms like fever and chills due to their ability to bind specifically to certain receptors located inside them's cytoplasm. They may cause damage if they get stuck into somatic tissues where it causes severe illnesses such as leukemia. By testing this type of protein through various methods, researchers could determine how well other drugs work towards treating diseases caused by microorganisms.

Problems solved by technology

This patented technical problem addressed in this patents relates to improving the accuracy and efficiency of testing for trepilmium virus diseases like sypharia through immunochemistry techniques such as flow cyscopy Microcampler® and Fluorimicroscopic Immunofluorescences technique(IFIT).

Method used

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  • High-throughput treponema pallidum specific antibody detection kit and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0037] see figure 1 , the embodiment of the Treponema pallidum-specific antibody high-throughput detection kit is provided with an outer packaging box 1, an alkaline phosphatase-labeled anti-human γ monoclonal antibody bottle 2, an alkaline phosphatase-labeled anti-human μ monoclonal antibody bottle 3 , alkaline phosphatase-labeled anti-human Ig monoclonal antibody bottle 4, luminescent substrate bottle 5, Treponema pallidum-specific antibody negative control bottle 6, Treponema pallidum-specific IgG antibody positive control bottle 7, Treponema pallidum-specific IgM positive 8 bottles of reference substance, 9 bottles of positive reference substance specific for Treponema pallidum specific antibodies, 10 bottles of washing solution, 11 bottles of recombinant antigen-coated microwell plates; 2 bottles of alkaline phosphatase-labeled anti-human γ monoclonal antibody, alkaline phosphatase-labeled Anti-human μ monoclonal antibody vial 3, alkaline phosphatase-labeled anti-human Ig...

Embodiment 2

[0064] The following shows the use of the Treponema pallidum-specific antibody high-throughput detection kit to detect the Treponema pallidum-specific antibody in clinical samples of patients:

[0065] 1. Specimen processing: Serum: 5mL of venous blood, placed in a 37°C water bath for 30 minutes, centrifuged at 3000g for 10 minutes, and the supernatant was used as a test sample for later use.

[0066] 2. Adding samples: add 100 μL of samples to the reaction plate, and make blank, negative and positive control wells at the same time. Incubate at 37°C for 1h.

[0067] 3. Washing: After reacting at 37°C for 30 minutes, the measured syphilis-specific antibody binds to the syphilis-specific recombinant antigen coated on the microwell plate, and the unbound free components are separated by washing;

[0068] 4. Add alkaline phosphatase-labeled anti-human gamma monoclonal antibody (to detect Treponema pallidum-specific IgG antibody), alkaline phosphatase-labeled anti-human μ monoclon...

Embodiment 3

[0070] The performance test of the Treponema pallidum-specific antibody high-throughput detection kit is given below

[0071] (1) Conformity rate of positive samples

[0072] Use 50 positive reference sera of syphilis-specific antibodies to test, and calculate the positive coincidence rate.

[0073] (2) Negative specimen coincidence rate

[0074] Use 50 samples of syphilis-specific antibody-negative reference sera to test, and calculate the negative coincidence rate.

[0075] (3) Intra-batch variance

[0076] The same batch of kits is tested with characteristic serum, requiring CV ≤ 10%.

[0077] (4) Difference between batches

[0078] Different batches of kits are tested with characteristic serum, requiring CV ≤ 12%.

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Abstract

The invention provides a high-throughput treponema pallidum specific antibody detection kit and a preparation method thereof, and relates to treponema pallidum. The kit comprises an outer packaging box, an alkaline phosphatase labeled antihuman gamma monoclonal antibody bottle, an alkaline phosphatase labeled antihuman mu monoclonal antibody bottle, an alkaline phosphatase labeled antihuman Ig monoclonal antibody bottle, a chemiluminescent substrate bottle, a treponema pallidum specific antibody negative reference substance bottle, a treponema pallidum specific IgG antibody positive reference substance bottle, a treponema pallidum specific IgM positive reference substance bottle, a treponema pallidum specific total antibody positive reference substance bottle, a washing liquid bottle and a recombinant antigen coated microporous plate. The preparation method of the kit comprises the steps of firstly preparing a treponema pallidum specific recombinant antigen, the recombinant antigen coated microporous plate, an antihuman gamma chain monoclonal antibody, an antihuman mu chain monoclonal antibody and an antihuman Ig monoclonal antibody, labelling the alkaline phosphatases of the antibodies, and then preparing a chemiluminescent substrate, washing liquid and reference substances, and finally, assembling the high-throughput treponema pallidum specific antibody detection kit.

Description

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Claims

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Application Information

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Owner ZHONGSHAN HOSPITAL XIAMEN UNIV
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