12 results about "Treponema palidum" patented technology

This invention discloses a recombinant adenovirus vaccine targeting the Tp0326 antigen and its preparation method, comprising a recombinant adenovirus vector and the Tp0326 antigen expressed therein. The recombinant adenovirus vector uses a replication-defective human adenovirus type 5 (Ad5) as a backbone, inserting the full-length Tp0326 antigen gene of *Treponema pallidum* into the adenovirus shuttle vector pshuttle-IRES-rGFP-1, and obtaining it through homologous recombination with the adenovirus backbone plasmid pAdEasy-1. This invention, using a replication-defective Ad5 as a vector, provides a recombinant adenovirus vaccine with high safety and efficient expression of the full-length Tp0326 antigen. The expressed antigen has a natural conformation, retains the ECL4 immunodominant epitope, and is more likely to induce a specific immune response, resulting in stronger immunogenicity. The preparation method involves constructing a recombinant adenovirus vector through homologous recombination, packaging it in HEK293T cells, and purifying it by CsCl density gradient centrifugation to obtain a high-purity, high-titer recombinant adenovirus vaccine suitable for large-scale production. The recombinant adenovirus vaccine can be administered via intramuscular or intranasal injection.

The invention relates to the technical field of mRNA vaccine research and development, and discloses a Tp0136T cell epitope mRNA vaccine based on lipid nanoparticle delivery and application of the Tp0136T cell epitope mRNA vaccine in syphilis prevention, the vaccine contains an mRNA sequence and an LNP delivery system, the mRNA sequence contains a Tp0136T1T cell epitope coding region (amino acid L477-S486, optimized by codon), a Cap 1 structure, optimized UTR and poly (A) tails of 65-76 adenosine, and psi or m5C is used for replacing trona; the LNP is prepared from SM-102, DSPC (Distearoyl Pyrrolidone), cholesterol and DMG-PEG (Dimethyl Glycol-Polyethylene Glycol) according to a molar ratio of 50 The particle size of the vaccine is less than or equal to 150nm, PDIlt; 0.2, the Zeta potential is-5 to-15 mV, and the encapsulation efficiency is greater than or equal to 93.47%. The preparation method comprises the steps of mRNA design synthesis, mRNA-LNP preparation characterization and in-vitro expression verification, through intramuscular injection inoculation, strong Th1 type and CD8 + CTL immune response can be induced, treponema pallidum load and skin ulcer rate can be reduced, and the method can be used for syphilis prevention.

The invention discloses a coater for clinical treponema pallidum non-specific antibody detection test. The coater comprises a main body hollow handle, a rubber coating sleeve and a standby liquid injector, wherein the main body hollow handle comprises a handle body, a handle piston rod, a liquid injection port and a main body needle head; the rubber smearing sleeve is integrally formed and comprises an outer wrapping connector and a smearing wafer. And the liquid injection part comprises an independent standby injector. The coater can store liquid, is large and uniform in coating surface and convenient to operate, the liquid outlet amount is convenient to adjust due to piston type liquid outlet, the needle head, the coating wafer and the like can be replaced, the problem of coating a serum specimen is effectively solved, and the coater is used for coating a treponema pallidum non-specific antibody detection test specimen.

The application provides a treponema pallidum antibody detection kit and application thereof, and relates to the technical field of biology.The treponema pallidum antibody detection kit comprises: a first reagent comprising mixed treponema pallidum membrane polypeptide coated negative charge gelatin particles; a second reagent comprising negative charge gelatin particles; a sample diluent; wherein the mixed treponema pallidum membrane polypeptide coated negative charge gelatin particles are prepared by mixing negative charge gelatin particles coated by four kinds of synthetic treponema pallidum membrane polypeptides with amino acid sequences of SEQ ID No.1, SEQ ID No.2, SEQ ID No.3 and SEQ ID No.4 respectively.The kit adopts the negative charge gelatin particles coated by the four kinds of synthetic treponema pallidum membrane polypeptides, and greatly improves the sensitivity and specificity of treponema pallidum antibody detection.

The present invention discloses a click chemistry-activated aggregation-induced fluorescent probe, a preparation method thereof, and a Treponema pallidum labeling method and application. The preparation steps include: reacting 4-bromotriphenylamine and 5-formyl-2-thiopheneboronic acid to obtain TPA-A, reacting TPA-A with ethynylmagnesium bromide to obtain an oily product TPA-TH with blue fluorescence, and then treating it with manganese dioxide to obtain 5-(4-(diphenylamino)phenyl)thiophene-2-alkynyl ketone (TPA-TA). Advantages of the present invention include: through the rational design and synthesis of a click chemistry-activated aggregation-induced fluorescent probe TPA-TA, it can then undergo a click chemistry reaction with amino proteins on the outer membrane of Treponema pallidum, thereby achieving stable labeling of T. pallidum.

The invention discloses a multifunctional bionic nanoparticle for syphilis light-driven treatment as well as a preparation method and application of the multifunctional bionic nanoparticle. The multifunctional bionic nano particle is of a structure that a core is coated with an outer membrane, the outer membrane is a cell membrane of macrophage pre-activated by treponema pallidum, and the core is a composite material with an AIE type photosensitizer. The multifunctional bionic nanoparticles provided by the invention can realize graded targeting of the ulcerative scleritis and pathogens in the ulcerative scleritis, and show accurate tracking of rash and fluorescence imaging of a near-infrared region I; under the irradiation of 808nm laser, the multifunctional bionic nanoparticles generate efficient photo-thermal and photodynamic effects, and treponema pallidum can be eliminated by photolysis, so that the non-invasive phototherapy of syphilis is realized. The multifunctional bionic nano-particle can be used as a bionic nano-drug for targeted therapy of syphilis, toxic and side effects of the drug are reduced, high-resolution imaging can be achieved, and the purpose of multi-mode comprehensive diagnosis and treatment is achieved.

The invention provides a method for detecting a treponema pallidum antibody based on a chemiluminescence method and application thereof, and the method comprises the following steps: mixing a to-be-detected solution with a magnetic bead suspension mother solution coated with a TP recombinant antigen, incubating to form a TP recombinant antigen-anti-TP antibody compound, cleaning to remove free components, the to-be-detected solution being a cerebrospinal fluid sample or a calibrator; adding an alkaline phosphatase-labeled TP recombinant antigen into the TP recombinant antigen-anti-TP antibody compound, incubating to form a TP recombinant antigen-anti-TP antibody-alkaline phosphatase-labeled TP recombinant antigen compound, and cleaning to remove free components; adding a luminous substrate into the TP recombinant antigen-anti-TP antibody-alkaline phosphatase labeled TP recombinant antigen compound, and detecting the luminous value of the cerebrospinal fluid sample; and judging whether the cerebrospinal fluid sample contains the treponema pallidum antibody or not according to the ratio of the luminous value of the cerebrospinal fluid sample to the Cut-off value of the calibrator. The invention provides powerful technical support for clinical diagnosis and monitoring of syphilis.

The invention discloses a multiplex PCR detection nucleic acid composition for treponema pallidum and herpes simplex virus, a detection product and application of the multiplex PCR detection nucleic acid composition and the detection product, and relates to the technical field of pathogen detection. According to the nucleic acid composition provided by the invention, four pathogen targets and one internal reference can be detected in the same reaction system by virtue of a fluorescent PCR instrument with four different fluorescent channels, and the nucleic acid composition has the technical advantages of high detection specificity, good typing effect and highly consistent detection results.

The present disclosure relates to a method for detecting and distinguishing between Treponema pallidum subspecies in a samples and distinguishing between venereal syphilis and non-venereal disease (e.g., yaws or bejel). Compositions, assays, and kits for use in the methods are also disclosed.

The present invention relates to an antigen combination comprising Treponema pallidum (Tp) antigens. The present invention also relates to vectors, polynucleotides, host cells and methods for producing such antigen combinations. The present invention also relates to method and uses of such antigen combinations. The present invention also relates to compositions, assays and kits and the methods and uses thereof.

An enzyme label used in an immunodetection of Treponema pallidum (TP) antibody, the enzyme label being a fusion protein of a labeling enzyme and a TP antigenic protein. A kit for detecting a TP antibody and a method for detecting an antibody produced after infection with Treponema pallidum (TP) in a sample, the kit including a fusion protein of a labeling enzyme and a TP antigenic protein. The antigenic protein contains one or more of TP15 antigen, TP17 antigen, TP47 antigen, and TP45 antigen, or a fusion antigen of two or more thereof.

The present invention relates to a kit for detecting sexually transmitted infection-causing pathogens selected from the group consisting of 12 sexually transmitted infection (STI)-causing pathogens, including Chlamydia trachomatis (CT), Neisseria gonorrhea (NG), Treponema pallidum (TP), Trichomonas vaginalis (TV), Ureaplasma urealyticum (UU), Ureaplasma parvum (UP), Mycoplasma genitalium (MG), Mycoplasma hominis (MH), Gardnerella vaginalis (GV), Candida albicans (CA), Herpes simplex virus 1 (HSV1), and Herpes simplex virus 2 (HSV2), and a method of detecting sexually transmitted infection-causing pathogens using the same.