86 results about "Treponema palidum" patented technology

A soft, pliable cellulose acetate phthalate (CAP)—hydroxypropyl cellulose (HPC) composite film is provided which is generated by casting from organic solvent mixtures containing ethanol. The film rapidly reduces the infectivity of several sexually transmitted disease pathogens, including the human immunodeficiency virus (HIV-1), herpesvirus (HSV), non-viral sexually transmitted disease pathogens (such as Neisseria gonorrhoeae, Haemophilus ducreyi, Chlamydia trachomatis and Treponema pallidum) and bacteria associated with bacterial vaginosis (BV). The film is converted into a gel/cream and thus does not have to be removed following application and use. In addition to being a topical microbicide, the film can be employed for the mucosal delivery of pharmaceuticals other than cellulose acetate phthalate.

A tablet for insertion into a vagina including 0.01 to 500 mg of a vaginal medication, such as a microbicide, such as cellulose acetate 1,2-benzenedicarboxylate (CAP); 100 to 500 mg of mannitol powder; 50 to 300 mg of inert microcrystalline cellulose; 10 to 80 mg of hydroxypropyl methylcellulose; 50 to 250 mg of glycerol and optionally 2 to 4 mg of at least one preservative which protects against microbicidal contamination and discourages the growth of yeast in the vagina. The tablet which includes CAP as the vaginal medication is vaginally administered before coitus in methods for preventing the sexual transmission of HIV-1, HIV-2, herpesvirus, or an infection caused by Neisseria gonorrhoeae, Chlamydia trachomatis, Trichomonas vaginalis, Haemophilus ducreyi or Treponema pallidum. The tablet which includes CAP as the vaginal medication is vaginally administered to prevent or treat bacterial vaginosis.

The invention belongs to the technical field of immunoassay and diagnosis and relates to a method for magnetic antibody immunoassay chemiluminescence detection of treponema pallidum antibodies. The method comprises the following steps of: using GoldMag particles as carriers, coating one or more antigens of specific recombinant proteins of treponema pallidum antibodies, TP15, TP17, TP47 and TP44.5, then adding a sample to be detected and enzyme labeled antigens, and finally adding luminescence substrates for luminescence detection. The GoldMag particle surface has a larger coupling capacity to the antigen and has characteristics of high specificity and no pollution of the enzyme linked immunoassay as well as high sensitivity of chemiluminescence, and therefore, the method has the advantages of high detection sensitivity, good specificity, wide linear range, no radioactive pollution and the like.

The invention relates to a treponema pallidum (TP) antibody test kit based on the flow microspheric carrier technology and a preparation method and detection method thereof, belonging to the technical field of immunoassay medical diagnosis. The preparation method comprises the following steps: using TP recombinant antigen to coat heavy polymer microsphere, using bovine serum albumin to seal empty binding site, preparing specific TP probe-heavy polymer microsphere; performing coculture with a sample to be tested to capture TP antibody, washing and centrifuging to remove the unbound TP antibody, then adding fluorescently-labeled anti-human IgG or IgM antibody; and using a flow cytometry to detect the fluorescence intensity of the microsphere, and performing qualitative or quantitative analysis to the tested antibody. The method has the advantages of high sensitivity and specificity and good stability and can be used to perform microanalysis or multivalent analysis to the sample.

The invention provides acquired immune deficiency syndrome virus and treponema pallidum fusion protein which can be used for preparing GICA (Gold Immunochromatographic Assay) one-step double-combined acquired immune deficiency syndrome and syphilis antibody rapid detection test paper, simultaneously and jointly detecting acquired immune deficiency syndrome and syphilis viruses, simultaneously carrying out combined detection on two diseases and carrying out result reading by naked eyes without special instruments, thereby providing a convenient, rapid, accurate and cheap detecting method for the site rapid detection, such as blood center street compulsory blood donor screening, family self-help detection, disease screening for frontier port exit and entry people, and the like, site epidemiology investigation and other mechanisms needing rapid detection.

The invention discloses a kit for detecting a treponema pallidum antibody as well as a detection method and an application thereof, and belongs to the technical field of in vitro diagnosis and detection. The kit consists of the following components: (1) a marker system: including a treponema pallidum recombinant antigen 1 in direct or indirect connection to a marking tracer, or staphylococcus aureus A protein in direct or indirect connection to the marking tracker; (2) a bridged compound system: including a treponema pallidum recombinant antigen 2 in direct or indirect connection to a first bridged compound; and (3) a magnetic microsphere system: comprising a magnetic microsphere in direct or indirect connection to a second bridged compound, wherein the first bridged compound is combined with the second bridged compound. The kit and the detection method disclosed by the invention can promote the full implementation of conjugation reaction between antigen and antibody; and detection sensitivity can be improved.

The invention relates to a kit, particularly a Treponema pallidum real-time fluorescence quantitative PCR (Polymerase Chain Reaction) multitarget detection kit and preparation thereof. The kit comprises a DNA (deoxyribonucleic acid) extraction reagent bottle, a heat-resistant DNA polymerase bottle, a PCR reaction liquid bottle, a standard substance bottle, a quality control substance bottle and apacking box, wherein the DNA extraction reagent bottle comprises a protease K bottle, a lysis buffer bottle, an anhydrous alcohol bottle, an inhibitor removing liquid bottle, a first cleaning buffer bottle, an eluent bottle, a second cleaning buffer bottle and a centrifugation column containing a collection tube; the standard substance bottle is provided with 6 standard substance plasmid bottles containing 5 target genes; and the quality control substance bottle comprises a negative quality control substance bottle and a positive quality control substance bottle. The preparation method comprises the following steps: screening the target genes, and constructing the multitarget gene standard substance plasmids; after making a multitarget gene detection reagent standard curve, sequentially preparing PCR reaction liquid and heat-resistant DNA polymerase; after establishing a sample processing method, preparing the quality control substance; and finally, completing the Treponema pallidum real-time fluorescence quantitative PCR multitarget detection kit.

The invention discloses a full-automatic ELISA detection device for treponema pallidum on a micro-fluidic chip. The full-automatic ELISA detection device comprises the micro-fluidic chip, a direct-current power source, a fluorescence detection system and a control system. The micro-fluidic chip comprises liquid storage tanks from first to seventh and a main channel, the liquid storage tanks from first to fifth are communicated with an inlet of the main channel through sample inlet channels, an outlet of the main channel is communicated with a sample outlet channel, and the other end of the sample outlet channel is communicated with the seventh liquid storage tank. The invention further discloses a detection method using the full-automatic ELISA detection device for treponema pallidum on the micro-fluidic chip. The full-automatic ELISA detection device has the advantages of being high in mechanization degree, simple in operation procedure, small in size, precise in structure, high in reliability and low in cost, and automatic control is convenient to achieve.

An antigen composition and method for the detection of antibodies to Treponema pallidum and the diagnosis of syphilis are described. The antigen composition contains synthetic cardiolipin and synthetic lecithin. The antigen composition may additionally contain cholesterol and an alcohol. The antigen composition is useful as an immunoreagent in immunoassays for the detection of antibodies associated with T. pallidum infection. The methods are sensitive and specific for T. pallidum infection.

The present invention generally features therapeutic and diagnostic compositions and methods for increasing or decreasing the binding of a lysozyme polypeptide to a Treponema pallidum P17 polypeptide (Tp17) or a Tp17-like polypeptide. More particularly, the invention relates to compositions and methods for detecting, treating, or preventing a pathogen infection or a chronic disorder; and to binding assays using a Tp17-like polypeptide and a lysozyme polypeptide.

A method for detecting luetin uses TP 0684 and TP 0453 syphilis specific antigens unitedly to make full use of characteristics and advantages of two said antigens for greatly raising sensitivity and specificity of detection. The kit for realizing said method to detect syphilis in early stage is also disclosed.

The invention relates to a treponema pallidum IgG antibody biotin avidin enzyme-linked immune detection kit and a preparation method thereof, which relates to the syphilis detection. The kit comprises a concentrated washing liquid bottle, a negative and positive control bottle, a biotin mark antihuman IgG specific fragment gamma chain monoclonal antibody bottle, a horseradish peroxidase mark avidin bottle, a TMB coloured solution bottle A, a coloured solution bottle B, a reaction stopping solution bottle, a micropore plate coated with recombinant antigen. The treponema pallidum has infectious standard strain Nichol strain and treponema pallidum non-infectious standard strain Reiter strain, by using a proteomics method, the treponema pallidum proteomics can be separated through dimensional electrophoresis, protein with strong immunogenicity can be identified by different patient or infectious animal serum, and then an antigen marker can be searched. The antigen marker is used for syphilis confirmation test, so that diagnosis sensitivity and specificity can be increased, a window stage is shortened. The treponema pallidum antibody can be sensitively detected and enables quantitative measurement, and has the advantages of cheap price, simple operation and accurate result.

The invention relates to treponema pallidum p15-17-47 fusion protein and a recombination expression method thereof, and belongs to the technical field of mechanical diagnosis of immunoassay. According to the technical scheme, the recombination expression method of treponema pallidum p15-17-47 fusion protein comprises the following steps in sequence: respectively performing PCR amplification for gene segments of p15, p17 and p47 three protein fragments in treponema Nihcols strains; analyzing effective epitopes of the three protein segments; amplifying and performing enzyme-cut and link up for the effective segments; building recombinant plasmid pPIC9k-p15-17-47 and recombinant saccharomycetes eukaryotic expressing system GS115pPIC9k-p15-17-47; performing liquid culture for recombinant yeast; and purifying recombinant expression products.

A TP nucleic acid testing kit mainly comprises a polymerase chain reaction (PCR) reaction reagent and a DNA extraction reagent. The testing kit is characterized in that the PCR reaction reagent comprises an upstream outer primer of specifically amplified TP nucleic acid, a downstream outer primer of the specifically amplified TP nucleic acid, a probe for detecting the TP nucleic acid, wherein the base sequence of the upstream outer primer is 5'-GGTCGATGTGCAAATGAGTGTT-3; The base sequence of the downstream outer primer is 5'-CCGACTTCAATACGGAGTTCAC-3'; the base sequence of the probe is 5'-ACTAGCCCTCCCTTCTACCTGAGATAAG-3'; and the 5'end of the probe is marked through fluorescent dye, and the 3'end of the probe is marked through quenching fluorescent dye. The testing kit has the advantages of being high in sensitivity, specificity and throughput, capable of detecting the TP rapidly and accurately, and suitable for clinical application and the like.

A Treponema pallidum triplet antigen construct is disclosed which includes three Treponema pallidum antigens (TP15, TP17, and TP47), as well as a ten amino acid leader sequence (tag 261) and human copper zinc superoxide dismutase (hSOD). This construct is optimized for in vitro diagnosis of syphilis infection. Plasmids containing DNA encoding the triplet antigen, host cells, production methods, detection methods, and kits are also disclosed.

The invention discloses an LAMP primer combination for detecting three ophthalmic infection spirochetes and application. The primer combination is composed of eighteen single-stranded DNA molecules shown from the sequence 1 to the sequence 18. The invention further provides application of the primer combination. The invention furthermore provides a method of using the primer combination for identifying whether a spirochete to be detected is treponema pallidum, or borrelia burgdorferi or leptospira, a method for identifying whether the spirochete to be detected is treponema pallidum, or borrelia burgdorferi or leptospira and a method for identifying whether a sample to be detected is infected by treponema pallidum and/or borrelia burgdorferi and/or leptospira. Treponema pallidum, borrelia burgdorferi and leptospira can be fast and accurately detected by using the method.

The invention relates to the technical field of immunodetection, and particularly relates to a syphilis test strip and a preparation method thereof, and a syphilis detection card and a preparation method thereof. The syphilis test strip comprises a bottom plate (1). The bottom plate (1) is provided with a sample pad (2), a glass cellulose membrane (3), a nitrocellulose membrane (4) and absorbent paper (5) in sequence. The glass cellulose membrane (3) is provided with a colloidal gold-marked treponema pallidum specific antigen and a colloidal gold-marked cardiolipin-phospholipid binding protein complex. A preparation method of the colloidal gold-marked cardiolipin-phospholipid binding protein complex comprises the steps of mixing cardiolipin and phospholipid binding protein; carrying out first incubation to obtain the cardiolipin-phospholipid binding protein complex; mixing the cardiolipin-phospholipid binding protein complex and colloidal gold; carrying out second incubation; and sealing to obtain the colloidal gold-marked cardiolipin-phospholipid binding protein complex. The detection card provided by the invention is simple and convenient to operate, has high sensitivity, high specificity and low false positive rate, and prevents interference of previous infection in current infection.

The invention discloses a rapid detective reagent strip of treponema pallidum immunoglobulin m (IgM) antibody. The rapid detective reagent strip of the treponema pallidum IgM antibody comprises an upper sampling pad (1), a colloidal gold pad (2), a NC film (3), an absorbing sampling pad (4) and a plastic plate (7). The colloidal gold pad (2) is marked with a T1 antibody and a TP fusion protein. An upper peridium man-resistant IgM mu chain monoclonal antibody of the NC film (3) forms a T line (5) and a sheep-resistant-mouse immunoglobulin G (IgG) antibody forms a C line (6). The upper sampling pad (1), the colloidal gold pad (2), the NC film (3) and the absorbing sampling pad (4) are pasted on the plastic plate (7) to form a detective reagent strip. Compared with the prior art, the rapid detective reagent strip of the treponema pallidum IgM antibody has the advantages of being high in sensitivity, strong in specificity and easy and convenient to operate. The rapid detective reagent strip of the treponema pallidum IgM antibody can provide an exact experimental basis for observation of curative effects after a clinical early stage diagnosis, an early treatment and an anti-luetic treatment.

The invention provides a high-throughput treponema pallidum specific antibody detection kit and a preparation method thereof, and relates to treponema pallidum. The kit comprises an outer packaging box, an alkaline phosphatase labeled antihuman gamma monoclonal antibody bottle, an alkaline phosphatase labeled antihuman mu monoclonal antibody bottle, an alkaline phosphatase labeled antihuman Ig monoclonal antibody bottle, a chemiluminescent substrate bottle, a treponema pallidum specific antibody negative reference substance bottle, a treponema pallidum specific IgG antibody positive reference substance bottle, a treponema pallidum specific IgM positive reference substance bottle, a treponema pallidum specific total antibody positive reference substance bottle, a washing liquid bottle and a recombinant antigen coated microporous plate. The preparation method of the kit comprises the steps of firstly preparing a treponema pallidum specific recombinant antigen, the recombinant antigen coated microporous plate, an antihuman gamma chain monoclonal antibody, an antihuman mu chain monoclonal antibody and an antihuman Ig monoclonal antibody, labelling the alkaline phosphatases of the antibodies, and then preparing a chemiluminescent substrate, washing liquid and reference substances, and finally, assembling the high-throughput treponema pallidum specific antibody detection kit.