Kit and method for detecting tp antibodies
By using a fusion protein of enzyme and TP antigenic protein in the detection of Treponema pallidum TP antibody, the problems of complex operation and non-uniform molar ratio of chemical activation cross-linking method are solved, achieving higher detection accuracy and simplified operation.
Patent Information
- Application Number
- CN201980101128.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2019-10-14
- Publication Date
- 2025-10-24
- Estimated Expiration
- 2039-10-14
AI Technical Summary
Among existing TP enzyme immunoassay methods, the chemical activation cross-linking method is complex and difficult to control, resulting in non-uniform molar ratios of enzyme and antigen molecules, which affects the production control of syphilis test kits and causes large batch-to-batch differences in test results.
A fusion protein of the enzyme and TP antigen was prepared by recombinant expression technology and used in an immunoassay kit for Treponema pallidum TP antibody, avoiding chemical activation and cross-linking.
A uniform molar ratio of labeling enzyme to TP antigenic protein was achieved, reducing batch-to-batch variability in test results, improving the differentiation between positive and negative samples and the sample concordance rate, and simplifying the operation process.