Colloidal gold test strip and test strip card for detecting IgM antibody, and preparation and detection method

A technology of colloidal gold test paper and colloidal gold, which is used in biological testing, measuring devices, material inspection products, etc.

Inactive Publication Date: 2016-01-20
THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The above 9 detection lines can be arranged in a

Method used

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  • Colloidal gold test strip and test strip card for detecting IgM antibody, and preparation and detection method
  • Colloidal gold test strip and test strip card for detecting IgM antibody, and preparation and detection method
  • Colloidal gold test strip and test strip card for detecting IgM antibody, and preparation and detection method

Examples

Experimental program
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Embodiment 1

[0057] This embodiment is used to illustrate the colloidal gold test strip for detecting IgM antibody of the present invention and its preparation method.

[0058] Such as figure 1 As shown, the colloidal gold test strip of the present embodiment comprises sample pads, conjugate pads, nitrocellulose membranes and water-absorbent pads attached successively on the polyvinyl chloride (PVC) base plate; Described sample pads are glass fiber membranes; The conjugate pad is a glass fiber membrane coated with a rabbit anti-human IgM antibody-colloidal gold conjugate; the nitrocellulose membrane is coated with 9 detection lines and 1 quality control line in turn, and the 9 detection lines The lines are Mycoplasma pneumoniae recombinant antigen, Chlamydia pneumoniae recombinant antigen, Influenza A virus antigen, Influenza B virus antigen, Parainfluenza virus antigen, Legionella pneumophila antigen, Q fever Rickettsia antigen, Respiratory syncytial virus antigen and Adenovirus antigens...

Embodiment 2

[0068] This embodiment is used to illustrate the colloidal gold test paper card and colloidal gold kit for detecting IgM antibody of the present invention and their preparation methods.

[0069] Such as figure 2 As shown, the colloidal gold test paper card includes a casing and a colloidal gold test strip located in the casing, and the colloidal gold test strip of this embodiment comes from Example 1. The housing is provided with a sample injection hole and a color development observation window, the sample injection hole is aligned with the position of the sample pad, and the color development observation window is aligned with the coating. There are 9 detection lines and 1 detection line. Position the nitrocellulose membrane for quality control lines.

[0070] The preparation method of this colloidal gold test paper card is: (1) prepare or purchase described housing (such as purchased from Hangzhou Hangan Technology Co., Ltd.); (2) pack the colloidal gold test paper strip ...

Embodiment 3

[0074] This embodiment is used to illustrate the method of using the colloidal gold test strip, colloidal gold test card or colloidal gold kit of the present invention for detecting nine respiratory tract infection pathogen-specific IgM antibodies.

[0075] (1) Collect the sample to be tested: the sample to be tested is serum, and when the serum is turbid, perform high-speed centrifugation to remove the turbid impurities.

[0076] (2) Put the colloidal gold test strip or colloidal gold test card flat on the test table, dilute the serum in step (1) 10 times and drop it on the sample pad, observe the test line and quality control within 10 to 15 minutes The color development result of the line; the color development result after 20 minutes and / or the quality control line does not show a red band, the test result is considered invalid and needs to be re-tested;

[0077] (3) According to the following table, determine the specific IgM antibodies of the nine respiratory infection p...

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Abstract

The invention provides a colloidal gold test strip for detecting an IgM antibody. The IgM antibody is a specific IgM antibody for nine respiratory tract infection pathogens, and the colloidal gold test strip comprises a sample pad, a conjugate pad, a nitrocellulose film and a water absorption pad which are attached to a polyvinyl chloride base plate in sequence; the conjugate pad is a glass fiber film wrapped with a rabbit-anti-human IgM antibody-colloidal gold conjugate; the nitrocellulose film is wrapped with 9 detection lines and 1 quality control line in sequence; the 9 detection lines are respectively a mycoplasma pneumoniae recombined antigen, a chlamydia pneumoniae recombined antigen, an influenza a virus antigen, an influenza B virus antigen, a sendai virus antigen, a legionella pneumophila antigen, a Coxiella burnetii antigen, a respiratory syncytial virus antigen and an adenovirus antigen, and the quality control line is a second antibody. The invention further provides a colloidal gold test strip card comprising the colloidal gold test strip and a colloid gold kit, a preparation method of the colloidal gold test strip card, and a method for realizing detection by adopting the colloidal gold test strip, the test strip card or the kit.

Description

technical field [0001] The invention relates to a test strip, in particular to a colloidal gold test strip for detecting nine specific IgM antibodies of respiratory tract infection pathogens, and a colloidal gold test strip and a colloidal gold test kit including the colloidal gold test strip. A preparation method of a colloidal gold test strip, and a detection method using the colloidal gold test strip, colloidal gold test card or colloidal gold test kit. Background technique [0002] At present, it is a mature technology to detect the specific IgM antibodies of nine respiratory pathogens in samples by immunofluorescence kits, but it takes a long time. For example, Antu Biological Company’s nine-item respiratory infection pathogen IgM antibody detection kit adopts the principle of indirect immunofluorescence, and is coated on glass slides (10 wells / slice): 1 well is coated with Legionella pneumophila serum type I, 2 Wells coated with Mycoplasma pneumoniae in McCoy cells, 3...

Claims

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Application Information

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IPC IPC(8): G01N33/558G01N33/68
CPCG01N33/6854G01N33/558
Inventor 蒋兴宇曹丰晶张伟
Owner THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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