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Colloidal gold chromatography strip for detecting specific IgM antibody and method for making same

A technology of colloidal gold and chromatography strips, applied in the field of chromatography strips, can solve the problems of easy false positives and low overall coincidence rate in detection results, and achieve the effects of good anti-interference ability and stability, and easy and fast operation.

Active Publication Date: 2007-08-08
北京英诺特生物技术股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method is difficult to avoid the influence of widely existing interfering substances such as rheumatoid factor RF, and the detection results are prone to false positives, and the overall coincidence rate is not high

Method used

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  • Colloidal gold chromatography strip for detecting specific IgM antibody and method for making same
  • Colloidal gold chromatography strip for detecting specific IgM antibody and method for making same
  • Colloidal gold chromatography strip for detecting specific IgM antibody and method for making same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1: Colloidal gold chromatography strip for detecting human cytomegalovirus (HCMV) IgM antibody

[0018] The specific preparation process is as follows:

[0019] (1) Prepare rabbit anti-HCMV antibody at a concentration of 5 mg / ml and mouse anti-human IgM monoclonal antibody at a concentration of 2 mg / ml by conventional methods, for use.

[0020] (2) Preparation of antigen-colloidal gold complex: use 0.1 mol / L K 2 CO 3 Adjust the pH value of the gold sol to 6.8, add 2.0 ml of the HCMV antigen at a concentration of 2 mg / ml to 100 ml of the gold sol, and stir for 10 minutes. Then add 10% bovine serum albumin 10.0ml, stir for 10 minutes, add 10% NaCl 10.0ml, and stir for 10 minutes. After centrifugation (1000 r / min) at 4°C for 10 minutes, the precipitation was removed. Centrifuge again (8000r / min) for 30 minutes and then remove the supernatant. The pellet was resuspended in 100.0ml of 20mM TBS, centrifuged at 4°C (8000r / min) for 30 minutes, and the supernatant was remove...

Embodiment 2

[0030] Example 2: Colloidal gold chromatography strips for detecting rubella virus (RV) IgM antibodies

[0031] A rabbit anti-RV antibody with a concentration of 4 mg / ml and a mouse anti-human IgM monoclonal antibody with a concentration of 2.5 mg / ml were prepared by conventional methods. The concentration of RV antigen added in the gold sol is 3 mg / ml. Other preparation processes and preparation parameters are the same as in Example 1.

[0032] The prepared colloidal gold chromatography strips were used to detect 186 positive sera and 2,452 negative sera with clear clinical diagnosis, and compared with the detection results of the capture method ELISA reagent. The results are shown in Table 3 and Table 4, respectively.

[0033] Table 3 Test results of colloidal gold chromatography strips on clinical serum

[0034] Table 4 Comparison of the results of colloidal gold detection and ELISA detection

[0035] From the data in Table 3, the Youden index can be calculated as 173 / (173+...

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PUM

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Abstract

The invention provides a colloidal gold chromatography and preparation technology to detect specific IgM antibody. The chromatography uses colloidal gold as the marker, and uses immunohistochemistry capture method to detect and determine IgM antibodies, and in PVC backplanes, sequentially and mutually attached with sample pad, composite material pad, nitro cellulose membrane, absorbing pad; the nitro cellulose membrane includes the detection area and the control area, the characteristics being: the said composite material pad coated with antigen-colloidal gold composite glass cellulose membrane, and the said detection area covers the anti-IgM antibody, and the said control area covers the antibody corresponding to the antigen.

Description

Technical field [0001] The invention relates to a chromatographic strip for detecting specific IgM antibodies by immunoassay, in particular, to a chromatographic strip for detecting specific IgM antibodies by means of immunochromatographic reaction developed by colloidal gold markers and the same Preparation. Background technique [0002] Immunoglobulin M (Immunoglobulin M, IgM) is the first antibody that appears in the immune response after the antigen stimulates the body. It has a short half-life. If specific IgM antibodies appear in the serum, it indicates that there has been a recent infection. Therefore, IgM antibodies can be used for humans. Or early diagnosis of animal diseases. Currently, enzyme-linked immunosorbent assay (Enzyme Linked Immunosorbent Assay, ELISA) is most commonly used to detect IgM. The ELISA method uses enzymes as markers and has good sensitivity and specificity, but the operating procedures are more complicated and the test time is longer. It generally...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558G01N33/544G01N33/577
Inventor 王志新王健陈廷友翟立伟孔祥菊毕大伟张秀杰
Owner 北京英诺特生物技术股份有限公司
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