Colloidal gold chromatography strip for detecting specific IgM antibody and method for making same

A technology of colloidal gold and chromatography strips, applied in the field of chromatography strips, can solve the problems of easy false positives and low overall coincidence rate in detection results, and achieve the effects of good anti-interference ability and stability, and easy and fast operation.
CN101013133AActive Publication Date: 2007-08-08北京英诺特生物技术股份有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
北京英诺特生物技术股份有限公司
Publication Date
2007-08-08

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Abstract

The invention provides a colloidal gold chromatography and preparation technology to detect specific IgM antibody. The chromatography uses colloidal gold as the marker, and uses immunohistochemistry capture method to detect and determine IgM antibodies, and in PVC backplanes, sequentially and mutually attached with sample pad, composite material pad, nitro cellulose membrane, absorbing pad; the nitro cellulose membrane includes the detection area and the control area, the characteristics being: the said composite material pad coated with antigen-colloidal gold composite glass cellulose membrane, and the said detection area covers the anti-IgM antibody, and the said control area covers the antibody corresponding to the antigen.
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Description

Technical field

[0001] The invention relates to a chromatographic strip for detecting specific IgM antibodies by immunoassay, in particular, to a chromatographic strip for detecting specific IgM antibodies by means of immunochromatographic reaction developed by colloidal gold markers and the same Preparation. Background technique

[0002] Immunoglobulin M (Immunoglobulin M, IgM) is the first antibody that appears in the immune response after the antigen stimulates the body. It has a short half-life. If specific IgM antibodies appear in the serum, it indicates that there has been a recent infection. Therefore, IgM antibodies can be used for humans. Or early diagnosis of animal diseases. Currently, enzyme-linked immunosorbent assay (Enzyme Linked Immunosorbent Assay, ELISA) is most commonly used to detect IgM. The ELISA method uses enzymes as markers and has good sensitivity and specificity, but the operating procedures are more complicated and the test time is longer. It generally...

Claims

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