Arg-gly-asp (RGD)-fused porcine circovirus 2 (PCV2) virus-like particles (VLPs), mutant infectious clone and preparation method of RGD-fused PCV2 VLPs, and application of RGD-fused PCV2 VLPs and infectious clone

A technology for porcine circovirus and type 2 virus, applied in the field of molecular biology and virology, can solve the problems of poor reduction ratio of viral load in pigs, low antibody titer, long immune blank period, etc., to enhance humoral immune response , the effect of increased antibody levels
CN110669142AActive Publication Date: 2020-01-10湖南派智生物科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
湖南派智生物科技有限公司
Publication Date
2020-01-10

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Abstract

The invention discloses arg-gly-asp (RGD)-fused porcine circovirus 2 (PCV2) virus-like particles (VLPs), an infectious clone and a preparation method of the RGD-fused PCV2 VLPs, and application of theRGD-fused PCV2 VLPs and the infectious clone. According to the RGD-fused PCV2 VLPs, RGD oligopeptides are fused on PCV2 Cap proteins. According to the RGD-fused PCV2 VLPs, the PCV2 Cap proteins are subjected to RGD polypeptide sequence oligopeptide modification, the RGD oligopeptides are fused on the PCV2 Cap proteins, and the PCV2 VLPs with RGD-fused on the surfaces are assembled in vitro. Compared with wild PCV2 VLPs, the levels of PCV2 specific IgM and IgG antibodies generated by RGD-fused PCV2 VLP immune mice are significantly increased, high-titer PCV2 antibodies can be generated in an induced mode, and the RGD-fused PCV2 VLPs enhance humoral immunity response. The RGD-fused PCV2 VLPs can be applied to development of enhanced VLPs and differential diagnosis molecular label VLPs vaccines, and a new idea is provided for research and development of PCV2 vaccines.
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Description

technical field

[0001] The invention relates to the fields of molecular biology and virology, in particular to a porcine circovirus type 2 (PCV2) virus-like particle and mutant infectious clone fused with RGD. In addition, the present invention also relates to a preparation method and application of porcine circovirus type 2 (PCV2) virus-like particles and mutant infectious clones comprising the fusion RGD. Background technique

[0002] Porcine circovirus (PCV for short) belongs to Circoviridae, is a kind of non-encapsulated, icosahedral single-stranded circular DNA virus, with a genome size of about 1.7Kb. There are currently three genotypes of PCV, namely porcine circovirus 1 (PCV1 for short), porcine circovirus 2 (PCV2 for short) and the newly discovered porcine circovirus type 3 (Porcine circovirus 1). circovirus 3, referred to as PCV3). According to relevant reports, PCV1 is not pathogenic, while PCV2 can cause many related diseases, and whether PCV3 is pathogenic is ...

Claims

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