Colloidal gold chromatography strip for detecting specific IgM antibody and method for making same

A technology of colloidal gold and chromatographic strips, which is applied in the field of chromatographic strips, can solve problems such as low overall coincidence rate and easy false positives in test results, and achieve good anti-interference ability and stability, and easy and fast operation

Active Publication Date: 2011-01-26
北京英诺特生物技术股份有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this method is difficult to avoid the influence of widely existing interfering substances such as rheumatoid factor RF, and the detection results are prone to false positives, and the overall coincidence rate is not high

Method used

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  • Colloidal gold chromatography strip for detecting specific IgM antibody and method for making same
  • Colloidal gold chromatography strip for detecting specific IgM antibody and method for making same
  • Colloidal gold chromatography strip for detecting specific IgM antibody and method for making same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1: detect the colloidal gold chromatography strip of human cytomegalovirus (HCMV) IgM antibody

[0018] Concrete preparation process is as follows:

[0019] (1) Rabbit anti-HCMV antibody at a concentration of 5 mg / ml and mouse anti-human IgM monoclonal antibody at a concentration of 2 mg / ml were prepared by conventional methods for future use.

[0020] (2) Preparation of antigen-colloidal gold complex: use 0.1mol / L K 2 CO 3 Adjust the pH value of the gold sol to 6.8, add 2.0 ml of HCMV antigen with a concentration of 2 mg / ml to 100 ml of the gold sol, and stir for 10 minutes. Add 10.0 ml of 10% bovine serum albumin, stir for 10 minutes, add 10.0 ml of 10% NaCl, and stir for 10 minutes. After centrifugation (1000r / min) at 4°C for 10 minutes, the precipitate was removed. Centrifuge again (8000r / min) for 30 minutes and remove the supernatant. The pellet was resuspended with 100.0ml of 20mM TBS, centrifuged at 4°C (8000r / min) for 30 minutes, and the supernat...

Embodiment 2

[0032] Embodiment 2: detect the colloidal gold chromatography strip of rubella virus (RV) IgM antibody

[0033] Rabbit anti-RV antibody at a concentration of 4 mg / ml and mouse anti-human IgM monoclonal antibody at a concentration of 2.5 mg / ml were prepared by conventional methods. The concentration of RV antigen added in the gold sol was 3 mg / ml. Other preparation process and preparation parameter are with example 1.

[0034] The prepared colloidal gold chromatography strip was used to detect 186 positive sera and 2452 negative sera with clear clinical diagnosis, and compared with the detection results of the capture ELISA reagent. The results are shown in Table 3 and Table 4, respectively.

[0035] Table 3 colloidal gold chromatography strips to the detection result of clinical serum

[0036]

[0037] Table 4 colloidal gold detection and ELISA detection result comparison

[0038]

[0039] From the data in Table 3, the Youden index can be calculated as 173 / (173+13)+24...

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PUM

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Abstract

The invention provides a colloidal gold chromatography and preparation technology to detect specific IgM antibody. The chromatography uses colloidal gold as the marker, and uses immunohistochemistry capture method to detect and determine IgM antibodies, and in PVC backplanes, sequentially and mutually attached with sample pad, composite material pad, nitro cellulose membrane, absorbing pad; the nitro cellulose membrane includes the detection area and the control area, the characteristics being: the said composite material pad coated with antigen-colloidal gold composite glass cellulose membrane, and the said detection area covers the anti-IgM antibody, and the said control area covers the antibody corresponding to the antigen.

Description

technical field [0001] The invention relates to a chromatographic strip for detecting specific IgM antibodies by means of an immunoassay, in particular to a chromatographic strip for detecting specific IgM antibodies by means of colloidal gold-labeled chromogenic immunochromatographic reactions and its Preparation. Background technique [0002] Immunoglobulin M (Immunoglobulin M, IgM) is the earliest antibody that appears in the immune response after the antigen stimulates the body. Or early diagnosis of animal diseases. Currently, enzyme-linked immunosorbent assay (Enzyme Linked Immunosorbent Assay, ELISA) is the most commonly used method for detecting IgM. The ELISA method uses enzymes as markers, which has good sensitivity and specificity, but the operation procedures are complicated and the test time is long. Generally, it takes 2.5 to 4 hours to obtain the test results, and a special enzyme immunoassay analyzer is required. Instruments such as a plate washer are not ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/544G01N33/558G01N33/577
Inventor 王志新王健陈廷友翟立伟孔祥菊毕大伟张秀杰
Owner 北京英诺特生物技术股份有限公司
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