Colloidal gold chromatography strip for detecting specific IgM antibody and method for making same

A technology of colloidal gold and chromatographic strips, which is applied in the field of chromatographic strips, can solve problems such as low overall coincidence rate and easy false positives in test results, and achieve good anti-interference ability and stability, and easy and fast operation
CN101013133BActive Publication Date: 2011-01-26北京英诺特生物技术股份有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
北京英诺特生物技术股份有限公司
Publication Date
2011-01-26

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Abstract

The invention provides a colloidal gold chromatography and preparation technology to detect specific IgM antibody. The chromatography uses colloidal gold as the marker, and uses immunohistochemistry capture method to detect and determine IgM antibodies, and in PVC backplanes, sequentially and mutually attached with sample pad, composite material pad, nitro cellulose membrane, absorbing pad; the nitro cellulose membrane includes the detection area and the control area, the characteristics being: the said composite material pad coated with antigen-colloidal gold composite glass cellulose membrane, and the said detection area covers the anti-IgM antibody, and the said control area covers the antibody corresponding to the antigen.
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Description

technical field

[0001] The invention relates to a chromatographic strip for detecting specific IgM antibodies by means of an immunoassay, in particular to a chromatographic strip for detecting specific IgM antibodies by means of colloidal gold-labeled chromogenic immunochromatographic reactions and its Preparation. Background technique

[0002] Immunoglobulin M (Immunoglobulin M, IgM) is the earliest antibody that appears in the immune response after the antigen stimulates the body. Or early diagnosis of animal diseases. Currently, enzyme-linked immunosorbent assay (Enzyme Linked Immunosorbent Assay, ELISA) is the most commonly used method for detecting IgM. The ELISA method uses enzymes as markers, which has good sensitivity and specificity, but the operation procedures are complicated and the test time is long. Generally, it takes 2.5 to 4 hours to obtain the test results, and a special enzyme immunoassay analyzer is required. Instruments such as a plate washer are not ...

Claims

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