34results about How to "The result is clear and easy to distinguish" patented technology

The invention provides a test strip for rapid detection of clostridium difficile toxins A and B. A monoclonal antibody or polyclonal antibody of the clostridium difficile toxin A, a monoclonal antibody or polyclonal antibody of the clostridium difficile toxin B, and a quality control double-antibody IgG coat a nitrate cellulose film (NC film), and a membrane chromatography double antibody sandwich method is adopted to detect the clostridium difficile toxins A and B in a specimen in combination with a monoclonal antibody of colloidal gold labeled clostridium difficile toxins A and B. The test strip is simple in operation, convenient, and fast, and has the advantages of no requirements of special instruments and special training, clear and identified result, and easy popularization. The test strip is suitable for base course, site detection and epidemiological investigation, and has auxiliary effect on the diagnosis of clostridium difficile toxin infection.

The invention provides a colloidal gold test paper strip for detecting a respiratory syncytial virus (RSV) antibody. In the colloidal gold test paper strip, the IgA antibody of the respiratory syncytial virus is detected by enveloping an antihuman IgA polyclonal antibody on a nitrocellulose membrane (NC membrane), and combining a respiratory syncytial virus specific recombinant antigen marked by colloidal gold and by using immune chromatography technology. The IgA antibody of the respiratory syncytial virus can be detected simply, conveniently, quickly and accurately by using the test paper strip, and a detection result is clear and easy to distinguish, so the test paper strip is suitable for batch detection and is suitable for grass-root screening and epidemiological survey, plays the role in assisting diagnosis of the early stage and medium stage of the infection of the respiratory syncytial virus and has a reference significance for judging whether children have the immunity of RSV resistance or not.

The invention belongs to the field of bio-detection and particularly relates to a C reactive protein saliva test paper strip and a preparation method thereof. The C reactive protein saliva test paper strip includes a reactive film and a gold marker pad. The reactive film is a nitrocellulose membrane, which has a detection line coated with a C reactive protein monoclonal antibody and a quality control line coated with goad-anti-mouse IgG. The gold marker pad is a polyester membrane that is coated with the C reactive protein monoclonal antibody marked by colloidal gold. The test paper strip, with human saliva as a detection sample, can effectively monitor the C reactive protein in saliva. The test paper strip has strong specificity and good repeatability, has high sensitivity, is 10 mg / L in lowest detection limit, is easy to operate and is free of special instruments and devices and professional training, has clear and distinguishable result and is easy to promote, and is suitable for on-site test.

The invention provides a test strip for rapid detection of a swine blue ear virus antibody. The swine blue ear virus antibody N protein, and anti swine blue ear virus antibody N protein polyclonal antibody are coated on a nitrate cellulose film (NC film), and a membrane chromatography double antigen sandwich method is adopted to detect the swine blue ear virus antibody in a swine serum, plasma, or whole blood specimen in combination with a colloidal gold labeled wine blue ear virus N protein. The test strip is simple in operation, convenient, and fast, and has the advantages of no requirements of special instruments and special training, clear and identified result, and easy popularization. The test strip is suitable for base course, large scale site detection of an accident and epidemiological investigation, and has auxiliary effect on the diagnosis of swine blue ear virus infection.

The invention discloses a test paper for rapidly detecting swine fever antibody and a preparation method thereof. The inventive test paper consists of a sample pad (4), a glass fiber member (3), a cellulose nitrate membrane (2), a water absorbent pad (1) and a support (5); wherein the cellulose nitrate membrane contains a detection line (6) coated by swine fever virus E2 protein and a reference line (7) coated with rabbit anti swine fever antibody; and the glass fiber member is combined with swine fever virus E2 protein marked by colloidal gold. The test paper can rapidly detect possibly present swine fever antibody in a sample to achieve rapid detection and timely epidemic control, thus creating favorable conditions for further separation and identification. The test paper has the advantages of convenient, rapid and easy usage, clear result, easy generalization, and no need for special instrument and equipment as well as professionals, and is suitable for large-batch onsite detection for base layer and for sudden events. The test paper is suitable for epidemic inquisition and performs assistant effect on swine fever virus infection diagnosis.

The invention provides a colloidal gold test strip for the detection of measles and rubella virus specific IgM antibodies. The measles specific gene engineering antigen H, the rubella virus specific gene engineering antigen E1, and a quality control double-antibody IgG are simultaneously coated on a nitrate cellulose film (NC film), and a membrane chromatography double antigen sandwich method is adopted to detect the measles and rubella virus specific IgM antibodies in a specimen in combination with a colloidal gold labeled anti-human IgM. The test strip is simple in operation, convenient, and fast, and has the advantages of no requirements of special instruments and special training, clear and identified result, and easy popularization. The test strip is suitable for base and site detection and epidemiological investigation, has auxiliary and differential diagnosis effects on measles and rubella virus infection, and can be used for the immune effect observation after vaccination.

The invention provides a colloidal gold test strip for detection of Streptococcus suis (serotype2). A monoclonal antibody of Streptococcus suis (serotype2) specific antibody MRP or polyclonal antibody of Streptococcus suis (serotype2) specific antigen, and a quality control second antigen IgG are coated on a nitrate cellulose film (NC film), and a membrane chromatography double antibody sandwich method is adopted to detect the Streptococcus suis (serotype2) specific antigen in a specimen in combination with a monoclonal antibody of 9i8kvbkikl colloidal gold labeled Streptococcus suis (serotype2) specific antibody MRP. The test strip is simple in operation, convenient, and fast, and has the advantages of no requirements of special instruments and special training, clear and identified result, and easy popularization. The test strip is suitable for base course, large scale site detection of an accident and epidemiological investigation, and has auxiliary effect on the diagnosis of Streptococcus suis (serotype2) infection.

This invention provides one glue gold test bar to test rabies virus protective antibody, which covers rabies virus protein and rabies multiple clone antibodies on the NC film and combines glue gold rabies virus antigen and applies film analysis double antigen clamper method and tests specimen rabies virus protective antibody.

The invention provides a rapid test strip for detecting an enterovirus 71 (EV71) IgM antibody. In the invention, EV71 gene antigenic vascular adhesion protein-1 (VAP1) and anti-mouse IgG are coated on a nitrocellulose (NC) membrane in combination with a colloidal gold labeled anti-human IgM monoclonal antibody, and the specific EV71 IgM antibody in an infected human specimen is detected by utilizing a membrane chromatography capture method. The rapid test strip provided by the invention has the advantages of being simple, convenient and rapid in operation, having no need of special instrumentand equipment and professional training, ensuring clear and easily-distinguished results and being easy for popularization; and the rapid test strip is applicable to a basic level as well as field test and early diagnosis, and plays a role in assisting the EV71 infection diagnosis.

The invention discloses a detection test paper. The test paper comprises (1) a reaction support, (2) a water absorption pad, (3) a nitrocellulose membrane, (4) a gold-labeled antibody adsorption membrane and (5) a sample pad, wherein the nitrocellulose membrane is coated with a detection strip and a quality control strip of a staphylococcal enterotoxin B antibody and a quality control antibody; and the gold-labeled antibody adsorption membrane contains the staphylococcal enterotoxin B antibody labeled by colloidal gold. The test paper can be matched with a small instrument to carry out semi-quantitative detection, does not need professional training, has clear and identifiable result, can objectively store data, is simple to operate, easy to popularize, suitable for a basic level, suitable for field detection of emergency and suitable for epidemiological investigation, and plays an auxiliary role in diagnosis of staphylococcal enterotoxin B infection.

This invention provides one rapid test bar for 01 cholera vibrio antigen, which covers 01 cholera vibrio An antigen single clone antibody or multiple clone antibody and quality IgG on NC film and combines glue gold label single clone antigen and applies film analysis double antibody clamper and tests the cholera vibrio antigen.

This invention provides one test bar, which comprises the following steps: a, covering A set of group wheel virus single clone antibody and IgG two ban NC fiber film; b, comprising glue gold label A set wheel single clone antibody glass fiber film; c, applying film analysis double antibody clamper to test A set wheel virus of feces.

The invention belongs to the field of bio-detection and particularly relates to a C reactive protein saliva test paper strip and a preparation method thereof. The C reactive protein saliva test paper strip includes a reactive film and a gold marker pad. The reactive film is a nitrocellulose membrane, which has a detection line coated with a C reactive protein monoclonal antibody and a quality control line coated with goad-anti-mouse IgG. The gold marker pad is a polyester membrane that is coated with the C reactive protein monoclonal antibody marked by colloidal gold. The test paper strip, with human saliva as a detection sample, can effectively monitor the C reactive protein in saliva. The test paper strip has strong specificity and good repeatability, has high sensitivity, is 10 mg / L in lowest detection limit, is easy to operate and is free of special instruments and devices and professional training, has clear and distinguishable result and is easy to promote, and is suitable for on-site test.

The invention provides colloidal gold rapid detection test paper for enterovirus 71 (EV71). The rapid detection test paper is characterized by coating the monoclonal antibody or polyclonal antibody of a specific antigen VP1 of EV71 and quality control anti-mouse IgG (immunoglobulin G) on a nitrocellulose membrane (NC membrane), and combining the monoclonal antibody of the specific antigen VP1 of EV71 marked by colloidal gold; and adopting a membrane chromatography double antibody sandwich method to detect the EV71 in a sample. The test paper provided by the invention can be used for detecting in a simple, convenient, rapid and direct mode without special apparatus and professional training; the test paper has a quite distinct result, is simple in operation and easy for popularization, and is suitable for basic level, suitable for the large scale field test for emergency, and suitable for early diagnosis, and plays an additional role for diagnosing the infection of the EV71.

The invention provides a test strip for fast detection of Streptococcus suis (serotype2), which comprises a reaction film and a conjugate release pad. The reaction film has a detection band coated with Streptococcus suis (serotype2) specific antigen MRP, and a quality control band of polyclonal antibody coated with anti Streptococcus suis (serotype2) specific antigen. The conjugate release pad is coated with colloidal gold labeled Streptococcus suis (serotype2) specific antigen, and a membrane chromatography double antigen sandwich method is adopted to detect the Streptococcus suis (serotype2) specific antigen. The test strip is simple in operation, convenient, and fast, and has the advantages of no requirements of special instruments and special training, clear and identified result, and easy popularization. The test strip is suitable for base course, large scale detection of an accident and epidemiological investigation, and has auxiliary effect on the diagnosis of Streptococcus suis (serotype2) infection.

The invention discloses a colloidal gold test strip for detecting a hepatitis E virus IgG antibody. The colloidal gold test strip comprises a reaction support, an absorbent paper pad, a nitrocellulosemembrane, a colloidal gold pad and a gold-labeled antigen protection membrane, wherein the colloidal gold pad is a glass fiber membrane containing colloidal gold-labeled hepatitis E virus ORF2 protein, and the nitrocellulose membrane is provided with two strips coated with a goat anti-human IgG antibody and a rabbit anti-HEV polyclonal antibody. According to the invention, the hepatitis E virus IgG antibody can be detected, and the method is simple, convenient, quick and accurate, does not need the special instruments and devices and the professional training, is clear in results and simple in operation, is easy to distinguish the results, is easy to popularize, is suitable for large-batch detection, is suitable for grassroots screening and epidemiological investigation, can play an auxiliary diagnosis role in the early stage and the middle stage of hepatitis E virus infection, and has the reference significance in judging whether a human body is infected with hepatitis E virus antigens recently or not.

This invention provides one test bar, which comprises the following steps: a, covering syncytium virus antibody of respiratory tract and IgG two band NC fiber film; b, comprising glue gold label respiratory syncytium virus single clone antibody glass fiber film; b, applying film analysis double antibody clamper principle to test nose secretion respiratory virus.

The invention discloses an anti-avian leukosis virus p27 protein monoclonal antibody, a gold-colloidal strip containing the same, and application. The anti-avian leukosis virus p27 protein monoclonal antibody is generated by secretion of a hybridoma cell strain with a preservation number of CGMCC NO. 11089 or a hybridoma cell strain with a preservation number of CGMCC NO. 11090. The monoclonal antibody is high in titer to p27 protein and good in specificity, and therefore can be used for preparing a kit and the gold-colloidal strip for detecting an avian leukosis virus. A preparation method of the monoclonal antibody provided by the invention is simple, and an antibody purification process is simple, and the efficiency is high, and the cost is low. As the gold-colloidal strip prepared by the anti-avian leukosis virus p27 protein monoclonal antibody provided by the invention is adopted to detect the anti-avian leukosis virus, the specificity is strong, and the operation is simple, and convenience, speediness and simplicity are realized, besides, a special instrument and equipment are not needed, and professional training is not needed, and a result is clear and easy to recognize; the operation is simple and convenient; the popularization is easy, and the antibody is more suitable for real-time detection.

The invention provides a test strip for rapid detection of a leptospira antibody, which comprises a reaction film and a conjugate release pad. The reaction film has a detection band simultaneously coated with Leptospira specific antigen ompL1 and LipL32, and a quality control band of polyclonal antibody coated with rabbit anti-Lebtospira specific antigen. The conjugate release pad is coated with colloidal gold labeled Leptospira specific antigens ompL1 and LipL32, and a membrane chromatography double antigen sandwich method is adopted to detect the Leptospira specific antigens ompL1 and LipL32 in a specimen. The test strip is simple in operation, convenient, and fast, and has the advantages of no requirements of special instruments and special training, clear and identified result, and easy popularization. The test strip is suitable for base and site detection and epidemiological investigation, and has auxiliary effect on the diagnosis of leptospiral infection.

The invention provides a test strip for rapid detection of a swine blue ear virus antibody. The swine blue ear virus antibody N protein, and anti swine blue ear virus antibody N protein polyclonal antibody are coated on a nitrate cellulose film (NC film), and a membrane chromatography double antigen sandwich method is adopted to detect the swine blue ear virus antibody in a swine serum, plasma, or whole blood specimen in combination with a colloidal gold labeled wine blue ear virus N protein. The test strip is simple in operation, convenient, and fast, and has the advantages of no requirements of special instruments and special training, clear and identified result, and easy popularization. The test strip is suitable for base course, large scale site detection of an accident and epidemiological investigation, and has auxiliary effect on the diagnosis of swine blue ear virus infection.

The invention relates to the technical field of immunoassay, and especially relates to a fluorescence immunoassay method of procalcitonin in saliva. A saliva collector, saliva, an anti-procalcitonin antibody, a fluorescence reagent and a detector are used in the invention. The saliva collector comprises one or a combination of a collecting tube, a collecting rod and a collecting scraper sheet; the anti-procalcitonin antibody is one or a combination of an anti-procalcitonin monoclonal antibody and an anti-procalcitonin polyclonal antibody; the fluorescence reagent is a liquid phase and / or solid phase material containing a fluorescent substance, and the fluorescent substance is one or a combination of organic fluorescence dyes or rare earth element fluorescence dyes; and the detector is a fluorescence detector. The method adopting human body saliva as a detection sample has the advantages of effective monitoring of procalcitonin in saliva, strong specificity, good repeatability, high sensitivity, simplicity in operation, no special devices, no professional training, clear and distinguishable result, easiness in promotion, and suitableness for onsite detection.

The invention provides a test strip for rapid detection of Japanese encephalitis virus IgM antibody. A Japanese encephalitis virus E gene antigen domain III and a double-antibody IgM III coat a nitrate cellulose film (NC film), and a membrane chromatography indirect sandwich method is adopted to detect the Japanese encephalitis virus specific IgM antibody in an infected human body specimen in combination with a colloidal gold labeled antihuman IgM monoclonal antibody. The test strip is simple in operation, convenient, and fast, and has the advantages of no requirements of special instruments and special training, clear and identified result, and easy popularization. The test strip is suitable for base course, site detection and epidemiological investigation, and has auxiliary effect on the diagnosis of Japanese encephalitis virus infection.

The invention provides a quick detection test strip for detecting leptospira IgM antibody, comprising a reaction film and a combination releasing pad; wherein the reaction film is provided with a detection zone capable of wrapping leptospira specific antigen ompL1 and LipL32 and a quality control zone encasing secondary antibody IgG, and the combination releasing pad is encased by anti-human IgM monoclonal antibody marked by colloidal gold. Leptospira specific IgM antibody in a specimen is detected by applying membrane chromatography capturing method. The test strip of the invention is applied to detection and is easy, convenient, fast and short-cut to operate, special instrument or equipment or professional training are not needed, the result is clear and easy to recognize, and the operation is easy, thus being easy to popularize, being applicable to basic level, field detection and early diagnosis, and being beneficial to leptospira infection diagnosis.

The invention provides a test strip for rapid detection of a Brucella specific antibody, which comprises a reaction film and a conjugate release pad. The reaction film has a detection band simultaneously coated with Brucella specific antigen bp26, and a quality control band of polyclonal antibody coated with Brucella outer-membrane protein bp26. The conjugate release pad is coated with colloidal golden labeled Brucella outer-membrane protein bp26. A membrane chromatography double antigen sandwich method is adopted to detect the Brucella specific antibody in a specimen. The test strip is simple in operation, convenient, and fast, and has the advantages of no requirements of special instruments and special training, clear and identified result, and easy popularization. The test strip is suitable for base course, large scale site detection of an accident and epidemiological investigation, and has auxiliary effect on the diagnosis of Brucella infection.

The invention provides ketamine-collaurum test paper for detection of saliva. The ketamine-collaurum test paper comprises a reaction film and a gold mark cushion, wherein according to the reaction film, a reaction line and a quality control line are formed by respectively coating ketamine and quality-control secondary antibodies IgG (immunoglobulin G) connected with carrier protein on a nitrocellulose film (NC film); the gold mark cushion is coated with ketamine monoclonal antibodies marked with collaurum; and a film chromatography competitive inhibition method is adopted to detect the ketamine in a saliva sample. The test paper can be used for effectively monitoring the ketamine in saliva, the detection specificity is strong, the repeatability is good, the sensitivity is high, and the lowest detection quantity reaches 50ng / ml. The test paper is simple to operate, does not need special instruments and equipment, does not need professional training and has clear and obvious results, thus being suitable for field detection.

The invention provides a test strip for simultaneous detection of measles and rubella virus specific IgG antibodies, which comprises a reaction film and a conjugate release pad. The reaction film has a detection band simultaneously coated with measles virus H antigen and rubella virus E1 specific antigen, and a quality control band coated with double-antibody IgG. The conjugate release pad is coated with colloidal gold labeled anti-human IgG. The test strip is simple in operation, convenient, and fast, and has the advantages of no requirements of special instruments and special training, clear and identified result, and easy popularization. The test strip is suitable for base and site detection and epidemiological investigation, has auxiliary and differential diagnosis effects on measles and rubella virus infection, and can be used for the immune effect observation after vaccination.

The invention provides a colloidal gold test strip for the detection of Leptospira. A monoclonal antibody of Leptospira specific antigen LipL32 or polyclonal antibody of anti Leptospira specific antigen, and a quality control double-antibody IgG coat a nitrate cellulose film (NC film), and a membrane chromatography double antibody sandwich method is adopted to detect the leptospira in a specimen in combination with a monoclonal antibody of colloidal gold labeled Leptospira specific antigen LipL32. The test strip is simple in operation, convenient, and fast, and has the advantages of no requirements of special instruments and special training, clear and identified result, and easy popularization. The test strip is suitable for base course, large scale site detection of an accident and epidemiological investigation, and has auxiliary effect on the diagnosis of leptospira infection.

The invention provides a colloidal gold test strip for the detection of Japanese encephalitis virus specific IgG antibody. A Japanese encephalitis virus E gene antigen domain III and an anti III polyclonal antibody are coated on a nitrate cellulose film (NC film), and a membrane chromatography double antigen sandwich method is adopted to detect the Japanese encephalitis virus specific IgG antibody in an animal or human body serum specimen in combination with a colloidal gold labeled Japanese encephalitis virus E gene antigen domain III. Or the Japanese encephalitis virus E gene antigen domainIII and an anti-mouse IgG are coated on the nitrate cellulose film (NC film), and a capture method is adopted to detect the Japanese encephalitis virus specific IgG antibody in the human body serum specimen in combination with a colloidal gold labeled antihuman monoclonal antibody. The test strip is simple in operation, convenient, and fast, and has the advantages of no requirements of special instruments and special training, clear and identified result, and easy popularization. The test strip is suitable for base course, site detection and epidemiological investigation, has auxiliary effecton the diagnosis of Japanese encephalitis virus infection, and can be used for the effect observation after vaccination.

The invention provides a detection test strip for a coxsackie virus A16 type (CA16) IgM (Immune Globulin M) antibody. According to the detection test strip, a nitrocellulose membrane (NC membrane) is coated by a CA16 gene antigen (VP1) and an anti-mouse IgG; and by combining a tihuman IgM monoclonal antibody labeled by colloidal gold, a CA16 specificity IgM antibody in an infected human speciment is detected by applying a membrane chromatography capture method. The test strip for detection, which is disclosed by the invention, has the advantages of simpleness, convenience, quickness and fastness in operation, no need of special instrument or special training, distinct and easily-distinguished result and easiness for popularization; and the detection test strip is suitable for field detection and early-stage diagnosis and assisting function for CA16 infected diagnosis.

The invention provides a test strip for rapid detection of clostridium difficile toxins A and B. A monoclonal antibody or polyclonal antibody of the clostridium difficile toxin A, a monoclonal antibody or polyclonal antibody of the clostridium difficile toxin B, and a quality control double-antibody IgG coat a nitrate cellulose film (NC film), and a membrane chromatography double antibody sandwich method is adopted to detect the clostridium difficile toxins A and B in a specimen in combination with a monoclonal antibody of colloidal gold labeled clostridium difficile toxins A and B. The test strip is simple in operation, convenient, and fast, and has the advantages of no requirements of special instruments and special training, clear and identified result, and easy popularization. The test strip is suitable for base course, site detection and epidemiological investigation, and has auxiliary effect on the diagnosis of clostridium difficile toxin infection.