Rapid test paper bar for testing colloidal gold of 01 group cholera vibrio

Abstract

This invention provides one rapid test bar for 01 cholera vibrio antigen, which covers 01 cholera vibrio An antigen single clone antibody or multiple clone antibody and quality IgG on NC film and combines glue gold label single clone antigen and applies film analysis double antibody clamper and tests the cholera vibrio antigen.

Description

technical field [0001] The invention belongs to the field of biological detection, and in particular relates to a test strip for O1 group Vibrio cholerae antigen detection and an application thereof. Background technique [0002] Cholera is an acute infectious disease caused by Vibrio cholerae infecting organisms, and its typical feature is massive loss of body fluid and electrolytes caused by severe diarrhea. Vibrio cholerae can be divided into more than 200 serogroups according to the specificity of its bacterial antigens, of which the most common and most infectious is the O1 group. [0003] At present, the traditional detection method of Vibrio cholerae O1 group is direct isolation and culture, isolation and culture after enrichment, etc. The operation is complicated and requires professional and technical personnel to carry out in a professional laboratory. It takes 16-24 hours and cannot handle a large number of suspicious specimens. Therefore, For the detection of Vi...

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Problems solved by technology

[0003] At present, the traditional detection method of Vibrio cholerae O1 group is direct isolation and culture, isolation and culture after enrichment, etc. The operation is complicated and requires professional and technical personnel to carry out in a professional laboratory. It takes 16-24 hours and cannot handle a large number of suspicious specimens. Therefore, For the detection of Vibrio cholerae O1 group, it is far from meeting the requirements of every diarrhea, and at the same time, the detection time cannot meet the purpose of rapid screening.

Such as PCR, latex agglutination test and monoclonal antibody diazo-based latex test (DPRC), although they can diagnose Vibrio cholerae within a few hours, with high specificity and sensitivity, but at the same time they have various Different kinds of defects, such as: high technical requirements, relatively expensive equipment, etc.

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