Colloidal gold test paper for staphylococcal enterotoxin B (SEB), preparation method thereof and application thereof
A technology of Staphylococcus aureus enterotoxin and colloidal gold, applied in biological testing, material inspection products, measuring devices, etc., can solve the problems of long detection time, cumbersome operation, unsuitable for on-site detection, etc., and achieve the effect of saving manpower and material resources
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0044] Embodiment 1, the preparation of the colloidal gold test paper that detects Staphylococcus aureus enterotoxin B type
[0045] 1. Preparation of colloidal gold probes
[0046] (1) HAuCl 4 It is first prepared as a 0.01% aqueous solution, and 100ml of pure water is taken and heated to boiling. Accurately add 1ml of 1% HAuCl under magnetic stirring 4 , while adding 1.5ml mass fraction of 1% trisodium citrate aqueous solution. After the color is stable, continue to heat and boil for 15 minutes. After cooling to room temperature, add pure water to make up to 100ml, and store in the dark at 4°C.
[0047] (2)K 2 CO 3 Adjust the PH value to about 8.2-8.5.
[0048] (3) Adjust the colloidal gold to a suitable pH, preferably 8.0-8.5, more preferably 8.2, and dilute the antibody to 0.2 mg / ml with 5 mM PB (pH 7.2).
[0049] 2. Preparation of gold-labeled antibody adsorption membrane
[0050] Take 1ml / tube of colloidal gold with pH 8.2 and distribute it in 1.5ml centrifuge tu...
Embodiment 2
[0054] Embodiment 2, Staphylococcus aureus enterotoxin type B detection test paper
[0055] Referring to Figure 1, the reaction support is a 6.2cm × 0.4cm PCV plate; the absorbent pad is 2cm × 0.4cm oil filter paper; the nitrocellulose membrane of 1.8cm × 0.4cm is coated with goat anti-rabbit IgG (purchased from Dingguo Biotechnology company, 1mg / ml), rabbit anti-SEB polyclonal antibody 2mg / ml (containing 1% BSA blocking agent), containing 0.4cm×0.4cm colloidal gold-labeled rabbit anti-SEB polyclonal antibody (concentration of standard gold: 5ug PBS dilution; Optimum pH value is 8.5) glass fiber membrane; sample pad is the glass fiber membrane of 2.7cm * 0.4cm; That is to form the staphylococcus aureus enterotoxin type B detection test paper.
Embodiment 3
[0056] Embodiment 3, detection method
[0057] See attached figure 2 , mix the sample to be tested with the sample diluent, and then use a pipette to add the sample mixture to the sample hole of the test paper. The liquid in the sample goes up by siphon action, and the result is interpreted in 10-15 minutes:
[0058] If it contains Staphylococcus aureus enterotoxin type B antigen, it will form a corresponding complex with the colloidal gold-labeled rabbit anti-SEB polyclonal antibody on the test paper, and combine with the flow rabbit anti-SEB polyclonal antibody coated on the nitrocellulose membrane to form a red color. line, that is, a red band is formed at the "T".
[0059] Regardless of whether it contains the corresponding antigen or not, the colloidal gold-labeled polyclonal antibody continues to crawl upward and forms a red precipitation line with the goat anti-rabbit IgG coated on the membrane, that is, a red band is formed at "C". This line is a quality control line...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com