7 results about "Staphylococcal Enterotoxins" patented technology

The present application relates to a kind of high-throughput staphylococcus aureus enterotoxin microfluidic chip detection method and application based on chain quantity amplification enhanced fluorescence.The microfluidic chip is provided with fluorescence sensor, and the fluorescence sensor includes identification probe and signal probe;The identification probe is composed of magnetic nanoparticle, nucleic acid aptamer H1 of target to be measured and target aptamer complementary chain H2, and the signal probe is composed of nanomaterial with signal amplification function and three oligonucleotides H3, H4, H5.The present application combines MNPs-ssDNA-AuNPs sandwich structure system and microfluidic chip, realizes detection signal amplification using the excellent superparamagnetic property of MNPs and the high loading capacity of AuNPs, constructs high-throughput, high-sensitivity fluorescence sensing technology for SEA, SEB and SEC1 detection, simplifies experimental process, and builds the rapid, efficient detection platform of multiple pollutants in food.

The invention provides a universal purification method of a recombinant staphylococcus aureus protein antigen. The purification method comprises the steps of sample treatment and refined purification. Wherein the sample treatment comprises fermentation material liquid crushing, crushed sample clarification and clarified sample concentration; the fine purification comprises primary purification, medium purification and fine purification; the recombinant staphylococcus aureus protein antigen is recombinant staphylococcus aureus enterotoxin A, enterotoxin B, enterotoxin C2 and / or toxic shock syndrome toxin 1. The purification method meets the quality standard required by pharmacopeia, also has the advantages of high yield, simple process, easy amplification and low cost, and greatly simplifies the production and preparation work of the recombinant staphylococcus aureus enterotoxin A, enterotoxin B, enterotoxin C2 and toxic shock syndrome toxin 1.

This invention provides a recombinant Staphylococcus aureus enterotoxin B nanoparticle protein vaccine, mSEB-mi3, its preparation method, and its applications. This invention utilizes a SpyTag / SpyCather protein linker system to connect the recombinant protein to the nanoparticle protein, allowing mSEB to be displayed on the surface of self-contained nanoparticles, thus obtaining the recombinant Staphylococcus aureus enterotoxin B nanoparticle protein mSEB-mi3. Both mSEB-SpyTag and SpyCather-mi3 proteins in this invention are induced to be expressed in soluble form in Escherichia coli. The mSEB-SpyTag and SpyCather-mi3 of this invention can be covalently bound via isopeptide bonds to form the mSEB-mi3 nanoparticle protein vaccine. The binding conditions are simple, and when combined with the adjuvant MF59, it can induce rapid antibody production in mice, generating a high-level immune response and exerting a protective effect. Furthermore, its antibody response rate and protective efficiency are superior to those of the monomeric protein mSEB.

The invention provides a photoelectrochemical aptamer sensor as well as a preparation method and application thereof, and relates to the technical field of biosensors, according to the photoelectrochemical aptamer sensor provided by the invention, a heterostructure of narrow-band-gap CdIn2S4 and WO3 is constructed, so that the prepared WO3 / CdIn2S4 composite material shows excellent photoelectrochemical signal response, and the photoelectrochemical aptamer sensor can be applied to the field of biosensors. According to the present invention, by combining the specific recognition effect of the aptamer, the sensitive analysis of the target object staphylococcal enterotoxin B (SEB) can be achieved, the sensor has characteristics of simple operation and strong anti-interference, the detection range is 1.0 * 10 <-17 >-1.0 * 10 <-12 > mol / L, the detection limit is as low as 1.26 * 10 <-18 > mol / L, and the sensor can be successfully used for the high-selectivity and rapid detection of the SEB in the water sample.

This invention relates to the fields of biomedicine and immunology, and particularly to the preparation and application of an anti-Staphylococcal enterotoxin B antibody. The antibody provided by this invention has a SEB-A8 heavy chain variable region containing CDR sequences as shown in SEQ ID NO:1-3, and a light chain variable region containing CDR sequences as shown in SEQ ID NO:9-11. It has significant application value in the treatment, prevention, and diagnosis of Staphylococcus aureus infections. Experimental data show that this antibody can effectively neutralize the superantigen activity of SEB, significantly improve the survival rate of MRSA-infected mice, and exhibit a dose-dependent protective effect. It can be used as a "non-antibiotic" treatment strategy for the treatment of methicillin-resistant Staphylococcus aureus infections, which is of great significance in controlling the development of bacterial resistance and provides a new research direction for clinical anti-infective therapy.

Provided is a formulation of an SEC1 mutant protein, a toxin of Staphylococcus aureus, exhibiting excellent effects on prevention, symptom· alleviation and treatment of mastitis via an improved immune function of lactating or non-lactating dairy Cows, comprising solid microparticles containing the SEC1 mutant protein as an active ingredient, a protein-stabilizing excipient, a carbohydrate-based auxiliary excipient and a lipophilic material, dispersed in a biocompatible oil and / or a fatty acid ester-based compound. The formulation of the present invention is capable of achieving effective in vivo delivery of a water-soluble mutant protein which is an active ingredient while maintaining activity thereof. In addition, the formulation of the present invention exhibits excellent therapeutic effects on prevention and treatment of mastitis via an enhanced immunopotentiating effects due to superior antibody-producing ability when administered to dairy cows. Further, thq. formulation of the present invention can be . used as an injectable preparation due to excellent injectability.

The invention provides an immunomagnetic bead method kit for enriching staphylococcus aureus enterotoxin B from a complex matrix, and belongs to the technical field of food detection. The invention discloses a kit for enriching staphylococcus aureus enterotoxin B (SEB). The kit comprises a carrier coupled with an anti-staphylococcus aureus enterotoxin B antibody and enrichment liquid, the enrichment liquid comprises a 0.05 M phosphate buffer solution containing 0.5%-1.2% of NaCl, 0.08%-0.12% of KCl and 0.08%-0.12% of Tween 20, and the pH value of the enrichment liquid ranges from 7.1 to 7.4. According to the kit, SEB in a to-be-detected sample is enriched under the condition of enrichment liquid through antibody coupling magnetic beads targeting SEB, so that the aims of concentrating and detecting a target substance and purifying the target substance are fulfilled, the detection sensitivity is effectively improved, and matrix interference is reduced.