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Application of SEC, antiviral vaccine adjuvant and composition

A vaccine adjuvant and compound technology, applied in the field of biological protein adjuvant and its preparation, can solve the problems of inability to induce cellular immune response, significant side effects, granulomas, etc., and achieve the effect of enhancing immune response and good immune effect. , the effect of increasing antibody titers

Inactive Publication Date: 2016-10-05
SHENYANG INST OF APPLIED ECOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the aluminum adjuvant immunizes the body, mainly based on the humoral immune response, and cannot induce the cellular immune response well, and in most viral infections, the cellular immune response has played a key role; although the oil-emulsion adjuvant can well Induce the body to produce humoral immune response and cellular immune response, but its side effects are also significant, mainly granuloma, redness and swelling often occur at the injection site

Method used

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  • Application of SEC, antiviral vaccine adjuvant and composition
  • Application of SEC, antiviral vaccine adjuvant and composition
  • Application of SEC, antiviral vaccine adjuvant and composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1: Preparation of Staphylococcus aureus enterotoxin C2

[0030] The purified proteins of positive clones Escherichia coli and Staphylococcus aureus enterotoxin C2 were preserved by our laboratory (for expression and purification methods, please refer to Xu Mingkai, Zhang Chenggang, Zhou Yafeng, Zhang Xianen. 2005. Gene cloning, expression and biological activity of Staphylococcus aureus enterotoxin C2 . Advances in Biochemistry and Biophysics (SCI). 32(3):275-281).

Embodiment 2

[0031] Embodiment 2: SEC2 immune BALB / c mice

[0032] 35 mice (6-8 weeks old female SPF level BALB / c mice purchased from Liaoning Changsheng Biotechnology Co., Ltd.) were divided into 5 groups, respectively SEC2 administration group, foot-and-mouth disease inactivated virus (FMDV Antigen) administration group , SEC2+Antigen administration group, commercial vaccine (porcine foot-and-mouth disease O-type inactivated vaccine O / MYA98 / BY / 2010 strain, purchased from Zhongnong Weite Biotechnology Co., Ltd.) administration group and normal saline negative control group. Immunize twice at intervals of two weeks, intramuscularly inject the left hind limb, and the injection dose is 200 μ L / rat. The dose of SEC2 in the compatible vaccine is 50 μg / bottle, and the dose of inactivated foot-and-mouth disease virus is 100 μg / bottle.

[0033] Mix 50 micrograms of SEC2 with 100 micrograms of FMD inactivated virus, dissolve in 200 microliters of normal saline, and prepare the final vaccine.

Embodiment 3

[0034] Embodiment 3: collecting serum

[0035] On day 0, day 5, day 10, day 14, day 28, day 42, day 56 and day 70 after the first immunization, blood was collected from the inner canthus of the mouse, and the blood sample was first placed at 4°C for 30 minutes , centrifuge at 5000 rpm at 4°C for 10 minutes to separate the serum, and store the serum at -70°C for ELISA detection.

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Abstract

The invention belongs to the field of biological products, and relates to a vaccine adjuvant, in particular to application of staphylococcus enterotoxin (SEC2) C2 to serving as a vaccine adjuvant and preparing a vaccine composition and a method. The SEC2 is adopted as the vaccine adjuvant, and experiments prove that the SEC2 can serve as a foot-and-mouth disease vaccine adjuvant. The SEC2 serves as the adjuvant, and compared with an existing commercial vaccine, the titer of an antibody and expression of cell factors IL-4 and IFN-gamma can be remarkably improved, and humoral immunity and cellular immune responses are stimulated more effectively. Meanwhile, the adjuvant is convenient to produec and transport and low in cost, and is convenient and easy to popularize when used for vaccine preparation.

Description

technical field [0001] The invention relates to a vaccine adjuvant and a preparation method thereof, and relates to a novel biological protein adjuvant and a preparation method thereof. Vaccine adjuvants required for immunization of different species of artiodactyla. Background technique [0002] With the overuse of antibiotics causing more and more serious problems, and the continuous emergence of new drug-resistant bacteria and viruses, infectious diseases have received close attention from medical workers again. In the field of prevention and control of infectious diseases, vaccination is still one of the most effective means. In this process, the addition of adjuvants can make the vaccine complex induce a stronger immune response and increase the antibody titer. Enhance the secretion level of cytokines, so the application of adjuvants is very important to enhance the immune system's response to vaccine antigens and improve the protective effect of vaccines. In traditio...

Claims

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Application Information

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IPC IPC(8): A61K39/39A61K39/135A61P31/14
Inventor 徐明恺孟北乾张惠文张成刚
Owner SHENYANG INST OF APPLIED ECOLOGY - CHINESE ACAD OF SCI
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