Staphylococcal enterotoxin detection method
A staphylococcal enterotoxin detection method technology, which is applied in the field of rapid and high-sensitivity staphylococcal enterotoxin detection, can solve the problems of long detection time and achieve high sensitivity, short detection time and wide application range
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Embodiment 1
[0034] In this embodiment, a detection chip with a metal silver structure with a characteristic size of 1000 nm on a quartz material substrate is used, and the detection method for staphylococcal enterotoxin of the present invention is used to detect SEB with a concentration of 10 μg / ml.
[0035] First, use quartz material as the chip substrate, clean it and perform hydrophilic treatment; make a cylindrical structure with an area of about 10mm×10mm, a characteristic size of 1000nm, and an array number of 10×10 on the surface of the substrate by photolithography; through gold plating, the Nanostructure metallization to obtain a chip with nanogold structures on the surface of the substrate. Then add 2mM dimercaptodiacetic acid aqueous solution dropwise on the surface of the sample, and react for 20min; add 0.4M ethylcarbodiimide hydrochloride EDC and 0.1M hydroxysulfosuccinimide NHS mixed solution dropwise On the surface of the detection chip, activate the carboxyl group for 2...
Embodiment 2
[0037] In this embodiment, a detection chip with a gold-silver composite structure with a characteristic size of 500 nm is used on a germanium material substrate, and the detection method for staphylococcal enterotoxin of the present invention is used to detect SEB with a concentration of 0.1 μg / ml.
[0038] First, germanium material is used as the chip substrate, cleaned and subjected to hydrophilic treatment; a five-pointed star structure with an area of about 500μm×500μm, a characteristic size of 500nm, and an array number of 4×4 is fabricated on the surface of the substrate by nanoimprinting; Silver metallizes the nanostructure to obtain a chip with a nanosilver structure on the surface of the substrate; then, a layer of 10nm thick gold is evaporated on the structure to form a double-layer composite structure. Then add 2mM dimercaptodiacetic acid aqueous solution dropwise on the surface of the sample, and react for 20min; add 0.4M ethylcarbodiimide hydrochloride EDC and 0...
Embodiment 3
[0040] In this embodiment, a detection chip with a gold structure with a characteristic size of 30 nm is used on a silicon material substrate, and the detection method for staphylococcus enterotoxin of the present invention is used to detect SEB with a concentration of 1 ng / ml.
[0041]First, silicon material is used as the chip substrate, cleaned and treated for hydrophilicity; on the surface of the substrate, diamond-shaped nanometers with an area of about 20 μm × 20 μm, a characteristic size of 30 nm, and a quadrilateral arrangement of 1 × 1 are fabricated by self-assembly of nanospheres. structure; the nanostructure is metallized by silver plating to obtain a chip with a nanometal structure on the surface of the substrate. Then add 2mM dimercaptodiacetic acid aqueous solution dropwise on the sample surface and react for 20min; add 0.4M ethylcarbodiacin hydrochloride EDC and 0.1M hydroxysulfosuccinimide NHS mixed solution dropwise On the surface of the detection chip, act...
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Abstract
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