133 results about "Toxin detection" patented technology

The present invention describes methods and cassettes for cell-based toxin detection and organ localization. The cassettes includes an array containing cells and a matrix of openings or depressions, wherein each region of the substrate enclosed by the opening or depression in the matrix forms a domain individually addressable by microfluidic channels in the device.

Methods and cassettes for cell-based toxin detection and organ localization are disclosed. The cassettes includes an array containing cells and a matrix of openings or depressions, wherein each region of the substrate enclosed by the opening or depression in the matrix forms a domain individually addressable by microfluidic channels in the device.

Methods, materials, and systems for detecting toxins are provided. In one aspect, a toxin contamination detector includes a substrate on which a bar code is printed. The bar code has a first color (e.g., black) that is effective to reflect light from a bar code scanning device to produce a bar code result. A toxin indicator is also included. The toxin indicator has a second color in the absence of toxin, which second color does not substantially affect or alter the bar code result. However, the toxin indicator presents a third color in the presence of toxin which substantially changes the bar code result; thereby indicating the presence of toxin.

The present invention describes methods and cassettes for cell-based toxin detection and organ localization. The cassettes includes an array containing cells and a matrix of openings or depressions, wherein each region of the substrate enclosed by the opening or depression in the matrix forms a domain individually addressable by microfluidic channels in the device.

Methods for microfabricating composite materials and composite materials prepared there from are described herein. The sacrificial material can be etched or patterned to create a two-dimensional and / or three-dimensional sacrificial material structure. The resulting sacrificial material structure can be embedded in one or more embedding materials. The sacrificial material(s) are materials whose solubility can be altered by application of a stimulus typically pH, and / or temperature, light, pH, pressure, presence of absence of ions, and combinations thereof. The embedding materials can contain one or more additives that modify one or more properties of the embedding materials, such as degradation properties, porosity, mechanical properties, viscosity, conductive properties, and combinations thereof. The composite materials can be used in tissue engineering, drug screening, toxin detection, drug delivery, filtrations, bioseparations, and as microfluidic devices for fluid mixing and structural repair.

A toxic material detection apparatus includes a sample collection portion including a sample inlet and a sample concentrator adapted to concentrate an environmental sample on a substrate. A sample distributing system transfers portions of the substrate to a color sensor and an ion mobility spectrometer for simultaneously analysis and toxin detection, particularly cholinesterase inhibitor detection. Optionally, a portion of the substrate may be directed to an archive for possible analysis at a later time. Reagents utilized include the enzyme acetylcholinesterase (AChE), and the reactants acetylthiocholine iodide (ATCI) and 5,5′-dithio-bis-(2-nitrobenzoic acid) (DTB). A data management unit provides for near-real-time analysis of the samples in under 5 minutes. Simultaneous “hits” by both analysis methods indicate the presence of a cholinesterase inhibitor.

The invention belongs to the field of molecularly imprinted polymers, and relates to a quercetin surface imprinted polymer and application of the quercetin surface imprinted polymer in detection of toxins of wheat and other crops. Quercetin is used as an alternative template of the polymer, UAO-66-NH2 is selected as a carrier material for surface imprinting, and the surface imprinted polymer is prepared by using a chemical grafting method. The synthesized polymer is used as an adsorbent to prepare a surface imprinted solid phase extraction column, and aflatoxin in food is extracted and separated, so that a simple, rapid, efficient and sensitive method for detecting aflatoxin in wheat samples is formed. When the separation enrichment effect is compared with that of commercial columns and immunoaffinity columns, aflatoxin can be adsorbed selectively, and the selectivity and adsorption capacity of aflatoxin are better; and furthermore, development of sample pretreatment for crop toxin detection is facilitated, and the problems of high cost and large time consumption of current pretreatment methods are solved.

The invention belongs to the technical field of toxin detection and discloses an SPR sensor for detecting microcystic toxin based on a nucleic acid aptamer signal amplification strategy and a preparation method and application thereof. The SPR sensor comprises a glass chip, a microcystic toxin aptamer, a DNA probe 1, a DNA probe 2 and a precious metal nano material, wherein microcystic toxin detection can be performed by determining SPR signal change. The preparation of the SPR sensor is simple and facilitates establishment of a portable detecting instrument for field monitoring, and the cost is far lower than that of a large analysis and detection instrument.

The invention discloses a monoclonal antibody capable of identifying T-2 and HT-2 toxin. The monoclonal antibody is secreted by a hybridoma cell T-2, which is preserved in China Center for Type Culture Collection, and has a preservation number of CCTCC NO:C201189. The invention also discloses an enzyme-linked immunosorbent assay method and a kit for detecting T-2 and HT-2 toxin, and application thereof to detection of HT-2 and T-2 toxin. Compared with prior art, the monoclonal antibody prepared by the invention has high identification sensitivity, good antibody specificity, IC50 values of 1.46 mug / L and 26.26 mug / L; the enzyme-linked immunosorbent assay method and the kit established by the invention have high detection precision and good accuracy; and a sample treatment method is simple and beneficial for rapid screening.

The invention discloses an immuomagnetic bead chromatographic test strip for rapidly detecting algae toxin and a preparation method thereof, relating to the technical field of algae toxin detection. The test strip consists of a lining board as well as a sample pad, a magnetic scale combination pad, a coated film and an absorbing pad which are sequentially engaged on the lining board; the magnetic scale combination pad is glass fiber for absorbing algae toxin magnetic scale antibody; and the coated film is provided with an adelomorphic detection line T line which is printed by algae toxin-coupled carrier protein solution, and an adelomorphic control line C line which is printed by goat anti-rabbit IgG solution. The sample pad of the test strip is inserted into the solution of a tested sample, then taken out after 10-20 seconds, and then reacts for 15 minutes at room temperature; the test strip is arranged in a magnetic signal detector for detection, and the detector further outputs the biological responding signals which have been converted into magnetic field signals in the form of electrical signals; and after a specification curve is drawn, the algae toxin content in the tested sample is calculated according to the specification curve. The invention relates to the method based on nano-magnetic immunological technique for detecting algae toxin, and has the characteristics of high sensitivity, short reaction time, cheap instrument and equipment, convenience, easy usage and the like.

A method for detecting zearalenone toxin in traditional Chinese medicines in different matrices, including extraction, purification and detection of Chinese medicine samples, said samples are extracted homogeneously at high speed with methanol-water (80:20, V / V), pH7. 0 Tween-PBS buffer solution dilution, glass fiber membrane filtration, immunoaffinity column purification. The determination method includes: high performance liquid chromatography-fluorescence detection method, high performance liquid chromatography-diode array detection method determination, spectrum and high performance liquid chromatography-mass spectrometry confirmation. Liquid chromatography conditions are: mobile phase ratio methanol: acetonitrile: water (8:46:46, V / V / V), fluorescence detector wavelength: excitation wavelength: 270nm, emission wavelength: 440nm; diode array detector wavelength: 236nm . Liquid quality conditions: Methanol-0.1% ammonium acetate water (75:25, V / V) as mobile phase, atmospheric pressure chemical ionization positive ion mode, drying gas flow rate 6.00L / min, drying gas temperature 340°C, fragmentation voltage 150V, Full scan mode scan, scan range 200-400m / z. The invention has the characteristics of accuracy, precision, reliability and the like.

The invention relates to a manufacture method and application of an immune sensor based on a polyaniline nano-particle composite membrane. By manufacturing the polyaniline nano-particle composite membrane, self-conductivity of materials is improved, and the membrane can be successfully applied to the immune sensor so as to greatly reduce detection limits of the sensor and achieve better stability. Representation and measurement of the sensor are performed through a cyclic voltammetry method and an alternate current impedance method, a staphylococcus aureus enterotoxin B detection standard curve is built, the linear range ranges from 0.1ng / ml to 8ng / ml, correlation coefficient R2=0.9932, and detection limit is 0.033ng / ml(S / N=3). The manufacture method and the application are good in specificity, can be used repeatedly, are good in stability, can be applied to fast detection of staphylococcus aureus enterotoxin B in dairy products and has wide application prospect, and dairy product detection recovery rate ranges from 84% to 111%.

The invention belongs to the technical field of photoelectrochemical sensors, and in particular relates to a gold nano particle-titanium dioxide nano wire array composite material as well as a preparation method and application thereof. The preparation method comprises the steps of adjusting the hydrolysis reaction rate through concentrated hydrochloric acid, wherien tetra-n-butyl titanate is taken as a titanium source and water is taken as a solvent under a hydrothermal system, and growing a titanium dioxide nano wire array on an FTO conductive glass-containing substrate; immersing the titanium dioxide nano wire array in a chloroauric acid solution, and heating and roasting the titanium dioxide nano wire array in air to obtain a gold nano particle-titanium dioxide nano wire composite structure; and performing chemical group and specific acceptor modification on the surfaces of gold nano particles to obtain the gold nano particle-titanium dioxide nano wire composite material. The composite material can be used for performing high-sensitivity bacterial toxin detection. The prepared photoelectrochemical sensor is low in cost, high in stability, high in photoelectric conversion efficiency and suitable for large-area production.

The invention relates to a measuring method of micro-capsule alga toxin in water. The method includes the steps as follows: firstly, compounding and appraising of the micro-capsule alga toxin; secondly, preparing and purifying of polyclonal antibody antibody: immunizing the New-Zealand rabbit by making the micro-capsule alga toxin holoantigen MC-LR-KLH as the immunity resource, and preparing the polyclonal antibody and purifying by ammonium sulphate according to the normal method; thirdly, preparing immune bead: coupling the micro-capsule alga toxin holoantigen MC-LR-BSA and the nanometer bead, and preparing the immune bead containing MC-LR-BSA; fourthly, embedding the antibody to the pyroxylin film; fifthly, producing testing board, combing the coupling mat of the magnetic scale MC-LR-BSA, the pyroxylin film embedding the polyclonal antibody, the sample mat, the sopping mat, the covering film, the testing board out card into a testing board; sixthly, sample testing, respectively adding standard goods and testing samples with different concentrations into the sampling holds on the testing board, the samples flow on the test paper through the chromatography effect, after 3 to 5 minutes of the reaction under the room temperature, the testing board is put into a magnetic single testing apparatus to be tested, and the testing apparatus can further output the biology reaction signals being transferred to the magnetic field signals by the way of the electric signals; after the standard curve being drawn, the specific value of the micro-capsule alga toxin content in the sample to be tested is counted based on the standard curve.

The invention belongs to the field of molecularly imprinted polymers and discloses an aflatoxin surface imprinted polymer and application thereof in crop detection. The surface imprinted polymer HKUST-1@MIPs is synthesized by using a metal organic framework material HKUST-1 as a carrier and 7-acetoxy-4-methylcoumarin as a substitute template of aflatoxin. The synthesized polymer is used as an adsorbent to prepare a surface imprinting solid-phase extraction column to extract and separate aflatoxin in food, and correspondingly a rapid, efficient and cheap aflatoxin sample pretreatment method isobtained; a detection method of aflatoxin in food, which is low in price, simple in operation and low in detection limit, is developed in combination with high performance liquid chromatography. The pretreatment development of crop toxin detection samples is facilitated, and the problems of high cost and large time consumption of current pretreatment methods are solved.

The invention discloses a piezoelectric detection sensor for microcystic toxins based on a molecular imprinting technology. The piezoelectric detection sensor is characterized in that a photochemistry polymerization method or an electrochemical polymerization method is utilized to compound a molecularly imprinted membrane, so that the combining capacity is improved, the delay time is shortened, and the specific adsorption capacity is strengthened; meanwhile, a quartz crystal serves as an effective apparatus of the sensor, so that the sensitivity of the sensor is improved, the stability is increased, and the cost is reduced. The piezoelectric detection sensor provided by the invention has the advantages that the property of the sensor is improved, the trace detection of the microcystic toxins is realized, and the microcystic toxins detection method which is high in sensitivity, high in speed, low in cost, and low in operation requirement is established.

The invention discloses a preparation method and a detection method of high-sensitivity paper capable of rapidly detecting algal toxin through Raman visualization and belongs to the technical field of nanometer materials and biochemical detection. The preparation method comprises the following steps: preparing an algal toxin antibody, preparing spherical and star-like nano particles, preparing a gold-labeled antibody with a Raman signal, assembling a paper-based Raman test strip and application in algal toxin detection. The invention provides a high-sensitivity paper-based Raman visualization method for rapidly detecting the algal toxin. The nano particles with the Raman signal are synthesized, the algal toxin antibody is labeled, and the method is used for performing high-sensitivity detection on the algal toxin by combining the immunogold labeling technique (ICA) with a Raman detection instrument.

The invention relates to preparation of a universal toxin paper detection sensor and application thereof and belongs to the technical field of toxin detection. The preparation comprises the following steps: packaging the traditional filter paper by utilizing a DIP-Dry technology through a carbon nanometer tube of a functional nanometer material during the preparation of the toxin paper detection sensor and adding an antibody of cyanobacterial toxin MC-LR into package solution to prepare the paper detection sensor capable of specially detecting the cyanobacterial toxin. The universal toxin paper detection sensor has the detection sensitivity of 0.6 ng / mL satisfying the limiting requirements of WHO on MC-LR in daily water, has equivalent detection sensitivity compared with the traditional ELISA method and leads the whole detection process not to exceed 0.5 hour, thereby greatly reducing the detection time, ensuring the detection quality and remarkably improving the detection efficiency simultaneously. The method is a universal detection method and can be used for conveniently preparing another universal toxin paper detection sensor only through replacing the antibody added during the preparation process of the sensor.

The invention discloses a carbon nano tube / nanogold composite membrane electrochemical immunosensor and application thereof, belonging to the technical field of electrochemical analysis. The surface of a glass carbon electrode is modified by a carbon nano tube, and a multi-potential-step method is adopted for depositing nano gold on the surface of the carbon nano tube, and thus the carbon nano tube / nanogold composite membrane is obtained; under the adsorption effect between nanogold and microcystic toxin-(leucine-arginine) antibody, an antibody is fixed on the surface of an electrode, a non-specific adsorption site is closed by utilizing bovine serum albumin, and an electrochemical immunosensor used for detecting microcystic toxin is researched; an immunoassay analysis mode of a 'sandwich'-structure is constructed by utilizing specific recognition effect between the microcystic toxin and an antibody thereof; and horse radish peroxidase labelled antibody is taken as a detection beacon, and detection on microcystic toxin is realized by utilizing a differential pulse voltammetry method. The carbon nano tube / nanogold composite membrane electrochemical immunosensor has high detection sensitivity, good selectivity and high accuracy when being used for microcystic toxin detection, and a new method is provided for analysis and study of microcystic toxin in water body.

The invention discloses a clostridial neurotoxin substrate protein, and a coding gene and an application thereof. The clostridial neurotoxin substrate protein is a protein (a) composed of an amino acid sequence represented by sequence 2 in a sequence table, or is a protein (b) obtained through substituting and / or deleting and / or adding one or more amino acid residues to the amino acid sequence represented by the sequence 2, related to the clostridial neurotoxin detection and derived from the sequence 2. The substrate protein SNVP provided by the invention has seven toxin serotype botulinum and tetanus toxin enzyme hydrolysis sites, can specifically identify A, B, E, C, D, F and G type botulinum toxins and tetanus toxins, and can be used for the detection and the parting discrimination of the botulinum toxins and tetanus toxins as a core detection reagent.

The invention discloses a staphylococcus aureus enterotoxin detection kit and preparation and use methods thereof; the kit includes staphylococcus aureus enterotoxin SEA and SEB specific antibodies and enzyme-labeled staphylococcus aureus enterotoxin SEA and SEB antibodies, has the characteristics of high specificity, high sensitivity, high precision, high accuracy and the like and can quickly detect melamine remaining in feeds and animal products.

The present invention is based on the use of 1) two polyclonal IgGs against two Cry toxins, 2) Cry toxin receptors isolated from lepidopteran larvae and 3) manual striping methods to manufacture immunochromatographic strips that are useful in detecting a variety of analytes. Immunochromatographic strips, which facilitate rapid detection of analytes are expensive if made using monoclonal antibodies and cannot be affordable for Indian farmers and extension workers. The main objective of the current method was to simplify the development of the immunochromatographic detection method for Cry (Bt) toxin detection using affinity purified polyclonal antibodies specific to the analyte and also to provide a robust and easy method suitable for manufacture of 'Cry1Ac / Cry1Ab / Cry2Aa / Cry2Ab toxin' detection strips at affordable cost, under Indian conditions. Anti-Bt (anti-Cry1 Ac or anti-Cry2Aa / Ab or both) antibody or Cry receptor proteins are immobilized on a cellulose nitrate membrane by manual striping. The membrane is blocked using casein or BSA and preservatives. Anti-Bt (anti-Cry1 Ac or anti-Cry2Aa / Ab or both) antibody is labeled with gold and adsorbed on glass-fibre membrane which is placed at the bottom the membrane so as to overlap 2mm. Specificity and accuracy of the assay are greatly enhanced due to the use of antigen-affinity and Protein-A affinity purified polyclonal IgG raised in two different animals (goat and rabbit). The schematic diagram of the Cry toxin detection lateral flow strip assembly is shown in a diagram appended herewith. The strips thus made represent rapid, sensitive devices and methods for detecting the presence of Cry1Ac / Cry1Ab / Cry2Aa / Cry2Ab and crystal toxins either from transgenic plants or in Bt-fermented products.

The invention discloses an MC-LR (microcystin-LR) detection method based on G-quadruplex nuclease, and belongs to the technical field of immunoassay chemistry. A quadruplex-hemin compound with similar peroxidase activity is successfully prepared and coupled to a gold nanoparticle surface, signals can be amplified, and finally, a gold nanoparticle-quadruplex conjugate is successfully marked on an antibody. By the aid of the novel antibody marker, a novel immunodetection method based on G-quadruplex DNA (deoxyribonucleic acid) is used for detecting microcystins. The method is simple, rapid and convenient, fine sensitivity and selectivity are embodied, detection sensitivity is improved by the signal amplification function of quadruplex, the microcystins in water can be simply, rapidly and highly sensitively detected, and international advanced level is reached.

The invention relates to a preparation method and a using method of a nodularin (NODLN) polyclonal antibody immunoaffinity column (IAC), and belongs to the technical field of immunoaffinity chromatography and algae toxin detection. In the prepared IAC, an NODLN polyclonal antibody is fixed in the column to adsorb NODLN in sample solution, enrichment and purification effects are achieved after elution, and qualitative and quantitative analysis is performed through high performance liquid chromatography (HPLC); in the pretreatment process, IAC enrichment is superior to the conventional solid-phase extraction (SPE) method; an HPLC graph shows that the NODLN polyclonal antibody IAC can separate the peak of the NODLN from the peak of other impurities so as not to influence a measurement result, and excessive impurities exist in SPE purification, so that the peak of the NODLN is even difficult to distinguish; and the NODLN polyclonal antibody can be specifically combined with the NODLN, so that the IAC has high specificity, and most interfering substances can be removed through one-time purification, while the SPE does not have specificity, and the interfering substances cannot be removed, so that the final measurement result is influenced.

The invention discloses an aflatoxin M1 gold label quick detection card and a preparation method and an application thereof, belongs to the field of immunology, and relates to a toxin detection technology. The detection card comprises a detection strip and a plastic card casing, wherein the detection strip is supported by a PVC (polyvinyl chloride) bottom lining and consists of a sample pad, a gold label antibody combination pad, an enveloping membrane and a water absorption pad which are sequentially connected; the gold label antibody combination pad is made of glass fibers, and envelops an aflatoxin M1 monoclonal antibody combined with colloidal gold particles; the enveloping membrane is a cellulose nitrate membrane and envelops a detection line (T line) containing recessive aflatoxin M1 protein conjugates and a control line (C line) containing a goat-anti-mouse monoclonal antibody; the sample pad is made of glass fibers processed by a buffering system. The detection card, based on the colloidal gold immunochromatograohic assay technology, is simple to operate, convenient to carry, and quick and accurate in result determination, requires only 40-50 minutes for detection, and is suitable for on-site supervision and qualitative screening of a great number of samples.

The invention discloses a rapid detection method for aflatoxin AFB1. According to the rapid detection method for aflatoxin AFB1, a DNA-CDs composite is prepared in a simple and green synthesis method,a low-cost novel fluorescent detection system for the aflatoxin is constructed based on the [pi]-[pi] stacking interaction between the humic acid and the DNA-CDs, the DNA-CDs is taken as a fluorescent probe, and the fluorescence of the AFB1 is directly utilized, so that the efficient and sensitive detection for the aflatoxin is achieved. This is the first time to use the readily available humic acid as a fluorescent quenching material for toxin detection. The rapid detection method for aflatoxin AFB1 is simple and convenient to operate, high in targeting property and sensitivity, and low in cost.

The invention relates to a preparation method for an aptamer biosensor for T-2 toxin detection in food or feed and application, which belong to the technical field of electrochemical detection. The method is characterized by comprising steps: a gold nanorod (Au NRs) material is firstly synthesized and obtained; platinum is then reduced on the gold nanorods to form gold platinum nanorods (Au@PtNRs), through combination with reducing graphene oxide-tetraethylenepentamine (rGO-TEPA), increasing of an immobilization amount is realized, and the signal amplification ability is improved; then, through combination with single-stranded DNA signal probe, a biosignal probe is thus prepared; and finally, through fixing of molybdenum disulfide-polyaniline-chitin-gold nanoparticle composite material anda DNA capture probe, an aptamer biosensor for T-2 toxin detection is thus prepared. The sensor is successfully applied to trace detection of T-2 toxin in food or feed. The method has the advantages that the limitation of a T-2 toxin small molecule substance is broken through, and through introducing a nucleic acid aptamer, T-2 toxin high-sensitivity and high-specificity trace detection can be realized, and a simple and quick new strategy is provided for mycotoxin detection.

A TaqMan / MGB probe PCR (polymerase chain reaction) technology can carry out bacterial genus identification on clostridium difficile in fecal genomes, simultaneously detect the carrying situation of toxin genes, and can judge whether a toxin A has deletion. A fecal specimen is not required to be purely cultured, and the strain identification and the toxin detection are completed in a reaction system. The method has the characteristics of simple operation, good repeatability, high flux detection specimens, short report time, and the like, and is applicable to the screening of pathogenesis of patients with diarrhea caused by clinically unexplained causes. The invention solves the technical problems that fecal specimens can be subjected to strain identification and strain toxin carrying situation screening simultaneously without being purely cultured, and a DNA (deoxyribonucleic acid) detection method for fecal genomes, which is high in sensibility, is provided. Because traditional anaerobic culture is uneasy to perform, and an enzyme immunoassay has methodology defects, according to the invention, the diagnosis rate of clostridium difficile associated diarrhea is significantly increased. Meanwhile, the invention also can be applied to the monitoring of drug used in the process of treating the diseases.