78 results about "Toxin detection" patented technology

The invention discloses a reaction buffer solution for detecting endotoxin by a recombinant C factor method and application of the reaction buffer solution. The reaction buffer solution comprises the following components: a basic buffer solution, magnesium chloride, trehalose and tween-20. The invention also discloses a preparation method of the reaction buffer solution and application of the reaction buffer solution in endotoxin detection. According to the reaction buffer solution for detecting the endotoxin by the recombinant C factor method, on the basis of ensuring the reaction sensitivity of the endotoxin, the anti-interference capability is remarkably improved, and the accuracy of detecting the endotoxin in an effective dilution range is ensured, so that the situation that the concentration of the endotoxin is lower than the detection limit due to excessive dilution, and the detection result is unreliable is avoided.

The invention discloses a T-2 toxin monoclonal antibody and application thereof, and belongs to the technical field of biology. The monoclonal antibody contains a heavy chain variable region and a light chain variable region, the heavy chain variable region and the light chain variable region are both composed of complementary determining regions and frame regions, and the complementary determining regions are both composed of CDR1, CDR2 and CDR3. The monoclonal antibody can be used for preparing a T-2 toxin detection product. The T-2 toxin colloidal gold test strip provided by the invention has the characteristics of high sensitivity, good specificity and strong stability.

The utility model discloses a rapid endotoxin detection device based on a recombinant C factor method, which comprises a shell with an opening on the front side; the kit is pushed into or pulled out of the shell through the opening by the bracket; the kit is placed on the bracket, a reaction cavity is formed in the kit, and a recombinant C factor reagent is stored in the reaction cavity; and the fluorescence analyzer is fixedly arranged above the kit in the shell. According to the endotoxin rapid detection device based on the recombinant C-factor method, recombinant C-factor reagents are added into the reagent storage area of the kit, the consistency of the recombinant C-factor reagents is high, batch difference does not exist, the accuracy is high when endotoxin detection is carried out, and the endotoxin rapid detection device is not limited by the yield of tachypleus amebocyte lysate. In the detection process, the recombinant C factor reagent is not exposed in the air, so that the influence of endotoxin in the air on the detection result is reduced.

The invention discloses light-driven toxin-enriched composite hydrogel as well as a preparation method and application thereof. The composite hydrogel comprises light-driven hydrogel and detection hydrogel, the light-driven hydrogel is agarose hydrogel doped with Au (at) Ag core-shell nano particles; the detection hydrogel is a double strand formed by an okadaic acid aptamer and a complementary sequence cDNA thereof, and a double strand formed by a domoic acid aptamer and a complementary sequence DNAzyme thereof, the hairpin H1 is used for modifying a quenching group BHQ2 at the 3'end, the hairpin H2 is used for modifying a fluorophore Cy3 at the neck part, the hairpin H3 is used for modifying a fluorophore FAM and a quenching group BHQ1 at the two ends respectively, and the metal ion doped agarose hydrogel is used for catalyzing DNAzyme cyclic shearing; and the photo-thermal driving unit is positioned on the detection function unit. According to the present invention, with the composite system of upper layer photo-thermal enrichment and lower layer dual-signal detection, the rapid and high-sensitivity simultaneous detection of the okadaic acid and the domoic acid is achieved through the combination of the photo-thermal transpiration effect and the HCR and DNAzyme cyclic shearing reaction;

The present invention relates to the field of antigen detection technology, and in particular to a nanobody for detecting Staphylococcus aureus enterotoxin B and its application. The nanobody comprises an amino acid sequence as shown in SEQ ID NO.1 or SEQ ID NO.2. The applications include: purification, detection, and removal of Staphylococcus aureus enterotoxin B. The present invention screened and obtained two nanobodies for SEB, which have high specificity and high affinity for SEB. Detection of SEB based on these two nanobodies has high specificity and sensitivity, which is of great value in the field of Staphylococcus aureus enterotoxin detection.

1. The name of the design product: portable bacterial toxin detection equipment. 2. The use of the design product: portable equipment for bacterial, toxin, and carcinogen detection. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: front view.

The present application belongs to the technical field of toxin detection, and particularly relates to a method for detecting shellfish toxin based on transition metal oxide / gold particle composite SERS substrate; paralytic shellfish poison, i.e. saxitoxin, in the shellfish toxin is selected as a detection object; the specific steps are as follows: firstly, a zinc oxide-silver composite nanoparticle SERS substrate is prepared to enhance the Raman signal of saxitoxin; then, SERS spectra of saxitoxin with different concentrations are collected; the collected spectra are introduced into matlab to establish a prediction model by using a PLS algorithm; an actual sample is treated according to a national standard method to obtain a treatment liquid; then, the SERS spectrum of the treatment liquid is collected and introduced into the prediction model to obtain a prediction result; the present application has the advantages of simple synthesis steps, good SERS enhancement effect, fast detection speed, high stability, and good application prospect in the technical field of harmful factor detection in food and the like.

The invention discloses a pretreatment toxin detection method and device based on agricultural products, and relates to the field of trace detection.The detection method comprises the following steps that S1, an agricultural product sample is homogenized, mixed with an ethanol-water solution containing amylase, subjected to an enzymolysis reaction and subjected to ultrasonic treatment, and a sample extracting solution is obtained; s2, adding an inorganic salt demulsifier, mixing, and centrifuging to obtain a sample filtrate; s3, loading the sample to a cation exchange solid-phase extraction column, sequentially leaching and eluting, and collecting an eluent; and S4, concentrating and redissolving, reacting with a color developing agent, measuring the absorbance at a specific wavelength, and calculating the toxin content. Alpha-amylase specific enzymolysis is adopted to destroy a starch net structure, toxin wrapping is avoided, the extraction efficiency is improved, starch colloid is effectively removed through salting-out demulsification and low-temperature centrifugation, a high-clarity extracting solution is obtained, matrix interference is effectively removed, and high-sensitivity and high-reliability visible light detection of trace toxins in high-starch agricultural products is achieved.

The invention discloses a bacterial endotoxin detection analyzer, and particularly relates to the technical field of biological detection, and the bacterial endotoxin detection analyzer comprises a detection instrument, an MCU microcontroller, a color development detection module, a position sensing module, a motor driving module, an automatic temperature control module, a data transmission and processing system and upper computer software. The MCU coordinates the operation of each module, the color development detection module is combined with an electrochemical impedance spectroscopy and an aptamer biosensor to quantify the endotoxin concentration, and the other modules are respectively responsible for sample frame positioning, sample positioning, temperature control, data interaction, human-computer interaction and the like. The technical effects and advantages are remarkable: various stable voltages can be efficiently converted, and the system stability is improved; high-sensitivity and high-specificity signal acquisition and concentration quantification are realized, and the detection accuracy is enhanced; signal jitter is eliminated, motor out-of-step stalling is prevented, and reliability is improved; the temperature fluctuation is maintained between-0.2 DEG C and + 0.2 DEG C, so that the biochemical reaction is stable and repeatable.

The invention discloses a preparation method and a detection method of a detection liquid for determining bacterial endotoxin in letemovir injection, which can accurately detect the original endotoxin in the letemovir injection by effectively destroying an inclusion body of the letemovir injection. A simple, reliable and accurate method is established for detecting the endotoxin in the letemovir injection, and strict quality control on the letemovir injection is facilitated.

The invention belongs to the technical field of fungaltoxin detection, and particularly relates to a Raman beacon COF nanoflower and a preparation method and application thereof. The method comprises the following steps: dissolving trialdehyde phloroglucinol, o-bitoluidine and polyvinylpyrrolidone in methanol, carrying out ultrasonic uniform mixing, adding a catalyst to carry out a reaction, adding benzaldehyde after the reaction is finished, continuously carrying out a standing reaction, centrifuging, washing and redissolving to obtain TpDBD-COF nanoflowers, dissolving the TpDBD-COF nanoflowers in a mixed solution of ethanol and water, adding AgNO3, carrying out ultrasonic adsorption, and carrying out vacuum drying to obtain the TpDBD-COF nanoflowers. After adsorption is finished, trisodium citrate is added for a heating reaction, centrifugation, washing and redissolution are performed, and the Raman beacon COF nanoflower is obtained. The COF nanoflower prepared by the invention has a highly enhanced stable Raman signal, the surface group has the advantage of being directly used for biomolecule coupling, and the COF nanoflower can be specifically used for sensitively detecting T-2 toxin in a sample.

The application discloses a chromogenic test paper for detecting aflatoxin and a preparation method thereof. The method adds montmorillonite to Fe(OH)3 colloid and mixes, and drops the mixture on a nitrocellulose membrane and waits for it to dry. The mixed solution of the Fe(OH)3 colloid and the montmorillonite in the application has an adsorption effect on the aflatoxin, forms a color block under the particle agglomeration effect, and realizes visual detection of the aflatoxin.

The invention discloses a fungaltoxin non-targeted screening mass spectrum data processing method based on deep learning, and relates to the technical field of food-borne toxin detection.The fungaltoxin non-targeted screening mass spectrum data processing method comprises the steps that on the basis of a standardized peak list, ion peaks serve as nodes, the mass spectrometry relation between the ion peaks serves as edges, and a mass spectrum structure is constructed; based on a connection relationship between nodes in the mass spectrum structure, identifying different mass spectrum relationship types, constructing different meta-paths from a starting ion peak to an ending ion peak, and generating a graph-level feature vector representing the whole mass spectrum structure; and performing multi-scale wavelet transform on the graph-level feature vector, obtaining a correction distance matrix by combining the distribution density value of the known mycotoxin category in the feature space, and extracting a multi-scale distance component corresponding to each known mycotoxin category for weighted fusion to obtain a minimum attribution distance. According to the method, deep semantic modeling of complex mass spectrum data is realized, so that the recognition accuracy of known toxins and the structural interpretability of unknown toxins are greatly improved.

The invention discloses an application of an unmarked fluorescent aptamer sensor in endotoxin detection, according to an unmarked aptamer probe, a guanine-rich nucleic acid sequence is introduced to two ends of an endotoxin specific aptamer, the unmarked aptamer probe is combined with thioflavin T (ThT) to present a fluorescence'open 'state, when the endotoxin exists, the fluorescence is weakened, and when the endotoxin exists, the guanine-rich nucleic acid sequence is introduced to the two ends of the endotoxin specific aptamer, the guanine-rich nucleic acid sequence is introduced to the two ends of the thioflavin T (ThT); a linear relation exists between the fluorescence intensity change and the endotoxin content, and the method can be used for quantitative detection of endotoxin. Under the optimal condition, the linear range of the detector is 10-80 ng / mL, the limit of detection (LOD) is 3.26 ng / mL, the recovery rate in drinking water and fruit juice standard samples reaches 93.34-113.29%, and the relative deviation is 1.37-6.44%. The constructed non-labeled fluorescent aptamer sensor has the characteristics of strong specificity, high sensitivity and the like, and provides a promising solution for detecting endotoxin in a food sample.

The present invention is directed to methods, compositions, and devices useful for detecting and / or quantifying microbial contaminants, including endotoxins. In embodiments, for absorbance-based assays, cartridges containing dry compositions useful in combination with handheld readers / devices are provided.

An in vitro method of detecting one or more endotoxins of one or more pathogens in a sample, comprising the steps of a. coating a lipid binding protein on a substrate or capturing a lipid binding protein on a capture molecule immobilized on a substrate, b. contacting the lipid binding protein, thus coated or captured, with the sample, c. detecting whether an endotoxin binds to the lipid binding protein, wherein the one or more endotoxins comprise a lipid A moiety and O- polysaccharide moiety, wherein the lipid binding protein is capable of binding the lipid A moiety of an endotoxin, and characterized in that the lipid binding protein is a mammalian protein and preferably is a murine or human protein.

The invention relates to a surface plasmon resonance (SPR) optical fiber probe, further relates to application of the SPR optical fiber probe in detection of type II ribosome inactivating protein (such as ricin and abrus precatorius toxin), further relates to a kit and a detection system comprising the SPR optical fiber probe, and further relates to a method for detecting the type II ribosome inactivating protein by using the SPR optical fiber probe. The metal nanoparticle layer on the surface of the SPR optical fiber probe is not easy to fall off, has good stability, can be repeatedly used, and can improve the detection stability.

This application belongs to the technical field of mycotoxin detection technology, specifically relating to a Raman beacon COF nanoflower, its preparation method, and its application. The method involves dissolving trialdehyde phloroglucinol, o-toluidine, and polyvinylpyrrolidone in methanol, ultrasonically mixing, adding a catalyst for reaction, adding benzaldehyde after the reaction, allowing the mixture to stand, centrifuging, washing, and redissolving to obtain TpDBD-COF nanoflowers. These nanoflowers are then dissolved in a mixed solution of ethanol and water, followed by the addition of AgNO3 and ultrasonic adsorption. After adsorption, trisodium citrate is added for heating reaction, centrifuging, washing, and redissolving to obtain the Raman beacon COF nanoflowers. The COF nanoflowers prepared by this invention exhibit a highly enhanced and stable Raman signal, and their surface groups have the advantage of being directly used for biomolecule coupling, specifically for the sensitive detection of T-2 toxin in samples.

The utility model relates to the technical field of detection cards, and discloses a biotoxin detection card which comprises a shell and detection test paper, the shell comprises a bottom shell and a shell cover detachably installed on the bottom shell, the upper side face of the bottom shell is concaved inwards to form a driving groove and a containing groove which are communicated, and the containing groove is used for containing the detection test paper. A limiting piece for limiting the test paper in the placing groove is arranged in the driving groove in a sliding manner; the detection test paper comprises a bottom plate, a sample pad, a colloidal gold pad, a nitrocellulose membrane and an absorption pad are sequentially arranged on the bottom plate, a detection line is arranged on the nitrocellulose membrane, a sample adding hole corresponding to the sample pad is formed in the shell cover, and an observation opening corresponding to the nitrocellulose membrane is formed in the shell cover. The shell cover is detachably mounted on the bottom shell, meanwhile, the limiting piece moves in the driving groove, and the detection test paper is limited in the containing groove, so that the detection test paper can be better taken and placed, namely, the detection test paper of the detection card can be replaced, and the shell is repeatedly used.

The invention discloses a neutralizing antibody targeting abrus precatorius toxin and application of the neutralizing antibody. Specifically disclosed is a monoclonal antibody or an antigen-binding fragment thereof targeting an abrine toxin, comprising a heavy chain variable region (SEQ ID NO: 1) and a light chain variable region (SEQ ID NO: 2). The monoclonal antibody has high affinity, can specifically target an A chain of Abrin-a toxin, can significantly inhibit Abrin-a induced cytotoxicity, has a significant protection effect on cells attacked by abrus precatorius toxin, can provide long-acting protection, and has good neutralizing ability. The monoclonal antibody can be prepared into products such as a therapeutic drug and a diagnostic drug for abrus precatorius toxin poisoning, a detection kit for abrus precatorius toxin and the like clinically. Based on a unique action mechanism and a remarkable treatment effect, the invention provides a novel biological preparation for preventing and treating Abrin poisoning, and has a wide clinical application prospect in the fields of first-aid medicine and biological defense.

This invention discloses a sample pretreatment method for detecting bacterial endotoxins in LNP-type mRNA vaccines. The method involves unpacking the sample with a 3-10% (v / v) Triton X-100 solution, followed by a 0.9% sodium chloride solution as the diluent in the sample gradient dilution step. This effectively eliminates interference from the positive control group in the gel limit method for detecting bacterial endotoxins in mRNA vaccines. The method provides accurate and reliable results and is suitable for the quality control of bacterial endotoxins in LNP-type mRNA vaccines.

The present invention is in the field of in vitro assays, particularly in the field of pyrogen and endotoxin detection. The invention provides improved compositions suitable for use in animal-free testing such as for use in a monocyte activation test. The improved compositions allow for test responses with increased sensitivity, improved dynamic range, and improved goodness of fit.

The invention relates to the technical field of toxin detection. The invention discloses a trace toxin detection device, system and method based on magnetic nanoparticles, the device comprises a box body, the box body is provided with an accommodating chamber, and the box body is provided with an opening; the sample holder is arranged in the box body, a to-be-tested sample tube is placed on the sample holder through an opening in the box body, a plurality of sample supports are arranged on the sample holder, and a to-be-tested sample and magnetic nanoparticles are placed in the to-be-tested sample tube; the driving structure is arranged on the box body, the sample frame is connected with the driving structure, and the driving structure is used for driving the sample frame to rotate; the fluorescence detection structure is arranged on the box body. According to the invention, when one to-be-detected sample is placed on one sample bracket, the sample bracket is driven to rotate, and the to-be-detected sample is detected, one to-be-detected sample can be placed on the other sample bracket through the opening in the box body, so that the time for taking out the previous to-be-detected sample and placing a new to-be-detected sample is saved; and the detection efficiency is improved.

The present application belongs to the field of analysis and detection, and particularly relates to a method for rapidly detecting algal toxins based on logic gates and a detection kit. The present application establishes a sensing platform for detecting algal toxins based on magnetic beads. Different algal toxin recognition probes and assembled hairpin probes are used to construct the MB detection platform. The assembled hairpin probes modified by fluorescent groups produce different fluorescent signals, which can be used to distinguish different algal toxins. Meanwhile, the sensing system of three logic gates, i.e. "AND-AND", "Feedforward circuit" and "Resource allocation circuit", provides a general sensing strategy for intelligent diagnosis of different algal toxins. The method simplifies the operation and reduces the cost. The whole detection process is fast in response, and the operation process can be mastered without professional training, which is convenient for rapid popularization and use.

The invention discloses an immune nucleic acid amplification detection kit and detection method based on MS2 virus-like particles, the kit contains the MS2 virus-like particles, the surface of the MS2 VLP displays an antigen conjugate capable of being specifically bound with an object to be detected, and a nucleic acid template is wrapped in the MS2 VLP; after the MS2 VLP is specifically combined with an object to be detected, detecting nucleic acid fragments wrapped in the combined MS2 VLP by adopting a recombinase polymerase amplification technology or real-time fluorescent quantitative PCR (Polymerase Chain Reaction), so as to improve the detection sensitivity of target protein. By utilizing the method, simple, highly sensitive and specific target molecule detection can be realized, the bottleneck of the traditional immunodetection method in sensitivity is broken through, and the problem of low coupling efficiency of the antibody and nucleic acid is effectively solved; the method is also suitable for the field of food safety (such as cereal toxin detection) and the field of clinical diagnosis (such as detection of low-abundance biomarkers in body fluid), and a new technical solution is provided for molecular detection.

The present invention discloses a biological toxin detection processor for meat food, which relates to the technical field of food detection devices. It includes a processing box. An ultrasonic extractor is arranged inside the processing box. A vibrating rod is installed at the output end of the ultrasonic extractor. Inside the processing box, a collection bottle body, a test tube and a base are arranged in sequence from top to bottom. A discharge pipe is connected to the lower end of the collection bottle body. A top cover is installed at the upper end of the collection bottle body. A screw blade is arranged inside the collection bottle body. A plurality of triangular pieces are arranged on the inner wall of the test tube. A lifting component and a translation component are installed on the outer side of the base; the translation component is used to push the base to slide directly below the vibrating rod, and the lifting component is used to raise the position of the base, so that the lower end of the vibrating rod penetrates into the test tube to perform ultrasonic oscillation work. After the extraction work is completed, the position of the base is lowered to separate the test tube from the vibrating rod, thereby achieving the effect of continuously processing meat food and avoiding contamination of the sample due to frequent transfer.

The invention discloses a method for eliminating interference in LNP type sample bacterial endotoxin detection. The method comprises the following steps: S1, determining a detection standard curve of a tachypleus amebocyte lysate dynamic color development method; s2, preparing a test sample solution A with different dispersing agent volume ratios and a test sample solution B added with the endotoxin with the midpoint concentration in the standard curve, and carrying out an interference elimination test by adopting the standard bacterial endotoxin solution C, the solution A and the solution B; s3, redissolving the tachypleus amebocyte lysate with bacterial endotoxin inspection water according to the specification of the tachypleus amebocyte S4, detecting by adopting a tachypleus amebocyte lysate dynamic color development method; and S5, judging the effectiveness of the experiment. According to the method for eliminating interference in bacterial endotoxin detection of the LNP type sample, the endotoxin dispersing agent is introduced, so that the multi-dimensional interference problems of endotoxin wrapped by lipid in the LNP sample, pH value deviation, osmotic pressure abnormity and the like are solved in a targeted manner.

The invention discloses a kit and a method for detecting abrus precatorius toxin. The kit comprises an elisa plate coated with an abrus precatorius toxin monoclonal antibody, an abrus precatorius toxin standard substance, an elisa antibody compound, a sample diluent, a washing solution, a developing solution and a stop solution, the kit adopts a double-antibody one-step sandwich method to realize the detection of the abrus precatorius toxin, and the double-antibody one-step sandwich method comprises the following steps: simultaneously adding an abrus precatorius toxin standard substance / sample and an enzyme-labeled antibody compound into micropores of an enzyme-labeled plate coated with an abrus precatorius toxin monoclonal antibody; and mixing and incubating to form an abrus precatorius toxin monoclonal antibody-abrus precatorius toxin-enzyme labeled antibody compound sandwich compound. The method disclosed by the invention has the characteristics of simplicity, rapidness, high sensitivity and the like, the detection range is 0-10 ng / mL, the lowest detection limit can reach 8.9 pg / mL, and the method is suitable for detecting the low-content abrus precatorius toxin of a large number of samples.

The invention belongs to the technical field of mould detection, and discloses a zearalenone, aflatoxin and vomitoxin detection method based on an xMAP technology, and the specific steps are as follows: S1, establishment of an antigen coating of the zearalenone xMAP detection method: coating a purified antigen with magnetic beads by using # imgabs0 # Antibody Coupling Kit; by utilizing the multi-labeling characteristic of the fluorescent encoding microspheres, multiple target molecules can be simultaneously identified in a single reaction, so that the sample collection quantity and processing steps are greatly reduced, and the tedious process of repeated experiments in a traditional method is avoided; by optimizing key parameters such as antigen coating conditions, working concentration of detection antibodies and signal amplification antibodies and the like, the sensitivity is remarkably improved on the premise of ensuring detection specificity, and meanwhile, a competitive immune test mode is combined with a high-throughput analysis instrument, so that single-time detection time is greatly shortened, the use amount of samples is extremely small, complex pretreatment is not needed, and the detection sensitivity is greatly improved. The operation process is standardized, simple and fast, and special equipment maintenance is not needed.

The invention relates to the technical field of toxin detection, in particular to a disc type full-automatic grain mycotoxin detector and a detection method thereof.The disc type full-automatic grain mycotoxin detector comprises a mixing pipe and a driving part, the power output end of the driving part is connected with the bottom of the mixing pipe, and a plurality of communicating mechanisms are arranged on the side wall of the mixing pipe in the circumferential direction; the liquid outlet ends of the communicating mechanisms are all connected with centrifugal tubes, and the communicating mechanisms enable the centrifugal tubes to communicate with the mixing tube under the action of rotating centrifugal force of the mixing tube. The detector adopts a centrifugal force and negative pressure suction mode to realize full-process automation from sample mixing, centrifugation to dilution and sample dropping detection, so that the detection efficiency is remarkably improved, and the detection error and the cross contamination risk are fundamentally eliminated.