52 results about "Toxin detection" patented technology

The invention discloses a T-2 toxin monoclonal antibody and application thereof, and belongs to the technical field of biology. The monoclonal antibody contains a heavy chain variable region and a light chain variable region, the heavy chain variable region and the light chain variable region are both composed of complementary determining regions and frame regions, and the complementary determining regions are both composed of CDR1, CDR2 and CDR3. The monoclonal antibody can be used for preparing a T-2 toxin detection product. The T-2 toxin colloidal gold test strip provided by the invention has the characteristics of high sensitivity, good specificity and strong stability.

The utility model discloses a rapid endotoxin detection device based on a recombinant C factor method, which comprises a shell with an opening on the front side; the kit is pushed into or pulled out of the shell through the opening by the bracket; the kit is placed on the bracket, a reaction cavity is formed in the kit, and a recombinant C factor reagent is stored in the reaction cavity; and the fluorescence analyzer is fixedly arranged above the kit in the shell. According to the endotoxin rapid detection device based on the recombinant C-factor method, recombinant C-factor reagents are added into the reagent storage area of the kit, the consistency of the recombinant C-factor reagents is high, batch difference does not exist, the accuracy is high when endotoxin detection is carried out, and the endotoxin rapid detection device is not limited by the yield of tachypleus amebocyte lysate. In the detection process, the recombinant C factor reagent is not exposed in the air, so that the influence of endotoxin in the air on the detection result is reduced.

The invention discloses light-driven toxin-enriched composite hydrogel as well as a preparation method and application thereof. The composite hydrogel comprises light-driven hydrogel and detection hydrogel, the light-driven hydrogel is agarose hydrogel doped with Au (at) Ag core-shell nano particles; the detection hydrogel is a double strand formed by an okadaic acid aptamer and a complementary sequence cDNA thereof, and a double strand formed by a domoic acid aptamer and a complementary sequence DNAzyme thereof, the hairpin H1 is used for modifying a quenching group BHQ2 at the 3'end, the hairpin H2 is used for modifying a fluorophore Cy3 at the neck part, the hairpin H3 is used for modifying a fluorophore FAM and a quenching group BHQ1 at the two ends respectively, and the metal ion doped agarose hydrogel is used for catalyzing DNAzyme cyclic shearing; and the photo-thermal driving unit is positioned on the detection function unit. According to the present invention, with the composite system of upper layer photo-thermal enrichment and lower layer dual-signal detection, the rapid and high-sensitivity simultaneous detection of the okadaic acid and the domoic acid is achieved through the combination of the photo-thermal transpiration effect and the HCR and DNAzyme cyclic shearing reaction;

1. The name of the design product: portable bacterial toxin detection equipment. 2. The use of the design product: portable equipment for bacterial, toxin, and carcinogen detection. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: front view.

The invention discloses a pretreatment toxin detection method and device based on agricultural products, and relates to the field of trace detection.The detection method comprises the following steps that S1, an agricultural product sample is homogenized, mixed with an ethanol-water solution containing amylase, subjected to an enzymolysis reaction and subjected to ultrasonic treatment, and a sample extracting solution is obtained; s2, adding an inorganic salt demulsifier, mixing, and centrifuging to obtain a sample filtrate; s3, loading the sample to a cation exchange solid-phase extraction column, sequentially leaching and eluting, and collecting an eluent; and S4, concentrating and redissolving, reacting with a color developing agent, measuring the absorbance at a specific wavelength, and calculating the toxin content. Alpha-amylase specific enzymolysis is adopted to destroy a starch net structure, toxin wrapping is avoided, the extraction efficiency is improved, starch colloid is effectively removed through salting-out demulsification and low-temperature centrifugation, a high-clarity extracting solution is obtained, matrix interference is effectively removed, and high-sensitivity and high-reliability visible light detection of trace toxins in high-starch agricultural products is achieved.

The invention discloses a bacterial endotoxin detection analyzer, and particularly relates to the technical field of biological detection, and the bacterial endotoxin detection analyzer comprises a detection instrument, an MCU microcontroller, a color development detection module, a position sensing module, a motor driving module, an automatic temperature control module, a data transmission and processing system and upper computer software. The MCU coordinates the operation of each module, the color development detection module is combined with an electrochemical impedance spectroscopy and an aptamer biosensor to quantify the endotoxin concentration, and the other modules are respectively responsible for sample frame positioning, sample positioning, temperature control, data interaction, human-computer interaction and the like. The technical effects and advantages are remarkable: various stable voltages can be efficiently converted, and the system stability is improved; high-sensitivity and high-specificity signal acquisition and concentration quantification are realized, and the detection accuracy is enhanced; signal jitter is eliminated, motor out-of-step stalling is prevented, and reliability is improved; the temperature fluctuation is maintained between-0.2 DEG C and + 0.2 DEG C, so that the biochemical reaction is stable and repeatable.

The invention discloses a preparation method and a detection method of a detection liquid for determining bacterial endotoxin in letemovir injection, which can accurately detect the original endotoxin in the letemovir injection by effectively destroying an inclusion body of the letemovir injection. A simple, reliable and accurate method is established for detecting the endotoxin in the letemovir injection, and strict quality control on the letemovir injection is facilitated.

The invention belongs to the technical field of fungaltoxin detection, and particularly relates to a Raman beacon COF nanoflower and a preparation method and application thereof. The method comprises the following steps: dissolving trialdehyde phloroglucinol, o-bitoluidine and polyvinylpyrrolidone in methanol, carrying out ultrasonic uniform mixing, adding a catalyst to carry out a reaction, adding benzaldehyde after the reaction is finished, continuously carrying out a standing reaction, centrifuging, washing and redissolving to obtain TpDBD-COF nanoflowers, dissolving the TpDBD-COF nanoflowers in a mixed solution of ethanol and water, adding AgNO3, carrying out ultrasonic adsorption, and carrying out vacuum drying to obtain the TpDBD-COF nanoflowers. After adsorption is finished, trisodium citrate is added for a heating reaction, centrifugation, washing and redissolution are performed, and the Raman beacon COF nanoflower is obtained. The COF nanoflower prepared by the invention has a highly enhanced stable Raman signal, the surface group has the advantage of being directly used for biomolecule coupling, and the COF nanoflower can be specifically used for sensitively detecting T-2 toxin in a sample.

The application discloses a chromogenic test paper for detecting aflatoxin and a preparation method thereof. The method adds montmorillonite to Fe(OH)3 colloid and mixes, and drops the mixture on a nitrocellulose membrane and waits for it to dry. The mixed solution of the Fe(OH)3 colloid and the montmorillonite in the application has an adsorption effect on the aflatoxin, forms a color block under the particle agglomeration effect, and realizes visual detection of the aflatoxin.

The invention discloses a fungaltoxin non-targeted screening mass spectrum data processing method based on deep learning, and relates to the technical field of food-borne toxin detection.The fungaltoxin non-targeted screening mass spectrum data processing method comprises the steps that on the basis of a standardized peak list, ion peaks serve as nodes, the mass spectrometry relation between the ion peaks serves as edges, and a mass spectrum structure is constructed; based on a connection relationship between nodes in the mass spectrum structure, identifying different mass spectrum relationship types, constructing different meta-paths from a starting ion peak to an ending ion peak, and generating a graph-level feature vector representing the whole mass spectrum structure; and performing multi-scale wavelet transform on the graph-level feature vector, obtaining a correction distance matrix by combining the distribution density value of the known mycotoxin category in the feature space, and extracting a multi-scale distance component corresponding to each known mycotoxin category for weighted fusion to obtain a minimum attribution distance. According to the method, deep semantic modeling of complex mass spectrum data is realized, so that the recognition accuracy of known toxins and the structural interpretability of unknown toxins are greatly improved.

The invention discloses an application of an unmarked fluorescent aptamer sensor in endotoxin detection, according to an unmarked aptamer probe, a guanine-rich nucleic acid sequence is introduced to two ends of an endotoxin specific aptamer, the unmarked aptamer probe is combined with thioflavin T (ThT) to present a fluorescence'open 'state, when the endotoxin exists, the fluorescence is weakened, and when the endotoxin exists, the guanine-rich nucleic acid sequence is introduced to the two ends of the endotoxin specific aptamer, the guanine-rich nucleic acid sequence is introduced to the two ends of the thioflavin T (ThT); a linear relation exists between the fluorescence intensity change and the endotoxin content, and the method can be used for quantitative detection of endotoxin. Under the optimal condition, the linear range of the detector is 10-80 ng / mL, the limit of detection (LOD) is 3.26 ng / mL, the recovery rate in drinking water and fruit juice standard samples reaches 93.34-113.29%, and the relative deviation is 1.37-6.44%. The constructed non-labeled fluorescent aptamer sensor has the characteristics of strong specificity, high sensitivity and the like, and provides a promising solution for detecting endotoxin in a food sample.

The present invention is directed to methods, compositions, and devices useful for detecting and / or quantifying microbial contaminants, including endotoxins. In embodiments, for absorbance-based assays, cartridges containing dry compositions useful in combination with handheld readers / devices are provided.

The invention relates to a surface plasmon resonance (SPR) optical fiber probe, further relates to application of the SPR optical fiber probe in detection of type II ribosome inactivating protein (such as ricin and abrus precatorius toxin), further relates to a kit and a detection system comprising the SPR optical fiber probe, and further relates to a method for detecting the type II ribosome inactivating protein by using the SPR optical fiber probe. The metal nanoparticle layer on the surface of the SPR optical fiber probe is not easy to fall off, has good stability, can be repeatedly used, and can improve the detection stability.

This application belongs to the technical field of mycotoxin detection technology, specifically relating to a Raman beacon COF nanoflower, its preparation method, and its application. The method involves dissolving trialdehyde phloroglucinol, o-toluidine, and polyvinylpyrrolidone in methanol, ultrasonically mixing, adding a catalyst for reaction, adding benzaldehyde after the reaction, allowing the mixture to stand, centrifuging, washing, and redissolving to obtain TpDBD-COF nanoflowers. These nanoflowers are then dissolved in a mixed solution of ethanol and water, followed by the addition of AgNO3 and ultrasonic adsorption. After adsorption, trisodium citrate is added for heating reaction, centrifuging, washing, and redissolving to obtain the Raman beacon COF nanoflowers. The COF nanoflowers prepared by this invention exhibit a highly enhanced and stable Raman signal, and their surface groups have the advantage of being directly used for biomolecule coupling, specifically for the sensitive detection of T-2 toxin in samples.

The invention discloses a neutralizing antibody targeting abrus precatorius toxin and application of the neutralizing antibody. Specifically disclosed is a monoclonal antibody or an antigen-binding fragment thereof targeting an abrine toxin, comprising a heavy chain variable region (SEQ ID NO: 1) and a light chain variable region (SEQ ID NO: 2). The monoclonal antibody has high affinity, can specifically target an A chain of Abrin-a toxin, can significantly inhibit Abrin-a induced cytotoxicity, has a significant protection effect on cells attacked by abrus precatorius toxin, can provide long-acting protection, and has good neutralizing ability. The monoclonal antibody can be prepared into products such as a therapeutic drug and a diagnostic drug for abrus precatorius toxin poisoning, a detection kit for abrus precatorius toxin and the like clinically. Based on a unique action mechanism and a remarkable treatment effect, the invention provides a novel biological preparation for preventing and treating Abrin poisoning, and has a wide clinical application prospect in the fields of first-aid medicine and biological defense.

This invention discloses a sample pretreatment method for detecting bacterial endotoxins in LNP-type mRNA vaccines. The method involves unpacking the sample with a 3-10% (v / v) Triton X-100 solution, followed by a 0.9% sodium chloride solution as the diluent in the sample gradient dilution step. This effectively eliminates interference from the positive control group in the gel limit method for detecting bacterial endotoxins in mRNA vaccines. The method provides accurate and reliable results and is suitable for the quality control of bacterial endotoxins in LNP-type mRNA vaccines.

The present invention is in the field of in vitro assays, particularly in the field of pyrogen and endotoxin detection. The invention provides improved compositions suitable for use in animal-free testing such as for use in a monocyte activation test. The improved compositions allow for test responses with increased sensitivity, improved dynamic range, and improved goodness of fit.

The invention relates to the technical field of toxin detection. The invention discloses a trace toxin detection device, system and method based on magnetic nanoparticles, the device comprises a box body, the box body is provided with an accommodating chamber, and the box body is provided with an opening; the sample holder is arranged in the box body, a to-be-tested sample tube is placed on the sample holder through an opening in the box body, a plurality of sample supports are arranged on the sample holder, and a to-be-tested sample and magnetic nanoparticles are placed in the to-be-tested sample tube; the driving structure is arranged on the box body, the sample frame is connected with the driving structure, and the driving structure is used for driving the sample frame to rotate; the fluorescence detection structure is arranged on the box body. According to the invention, when one to-be-detected sample is placed on one sample bracket, the sample bracket is driven to rotate, and the to-be-detected sample is detected, one to-be-detected sample can be placed on the other sample bracket through the opening in the box body, so that the time for taking out the previous to-be-detected sample and placing a new to-be-detected sample is saved; and the detection efficiency is improved.

The present application belongs to the field of analysis and detection, and particularly relates to a method for rapidly detecting algal toxins based on logic gates and a detection kit. The present application establishes a sensing platform for detecting algal toxins based on magnetic beads. Different algal toxin recognition probes and assembled hairpin probes are used to construct the MB detection platform. The assembled hairpin probes modified by fluorescent groups produce different fluorescent signals, which can be used to distinguish different algal toxins. Meanwhile, the sensing system of three logic gates, i.e. "AND-AND", "Feedforward circuit" and "Resource allocation circuit", provides a general sensing strategy for intelligent diagnosis of different algal toxins. The method simplifies the operation and reduces the cost. The whole detection process is fast in response, and the operation process can be mastered without professional training, which is convenient for rapid popularization and use.

The invention discloses an immune nucleic acid amplification detection kit and detection method based on MS2 virus-like particles, the kit contains the MS2 virus-like particles, the surface of the MS2 VLP displays an antigen conjugate capable of being specifically bound with an object to be detected, and a nucleic acid template is wrapped in the MS2 VLP; after the MS2 VLP is specifically combined with an object to be detected, detecting nucleic acid fragments wrapped in the combined MS2 VLP by adopting a recombinase polymerase amplification technology or real-time fluorescent quantitative PCR (Polymerase Chain Reaction), so as to improve the detection sensitivity of target protein. By utilizing the method, simple, highly sensitive and specific target molecule detection can be realized, the bottleneck of the traditional immunodetection method in sensitivity is broken through, and the problem of low coupling efficiency of the antibody and nucleic acid is effectively solved; the method is also suitable for the field of food safety (such as cereal toxin detection) and the field of clinical diagnosis (such as detection of low-abundance biomarkers in body fluid), and a new technical solution is provided for molecular detection.

The invention discloses a kit and a method for detecting abrus precatorius toxin. The kit comprises an elisa plate coated with an abrus precatorius toxin monoclonal antibody, an abrus precatorius toxin standard substance, an elisa antibody compound, a sample diluent, a washing solution, a developing solution and a stop solution, the kit adopts a double-antibody one-step sandwich method to realize the detection of the abrus precatorius toxin, and the double-antibody one-step sandwich method comprises the following steps: simultaneously adding an abrus precatorius toxin standard substance / sample and an enzyme-labeled antibody compound into micropores of an enzyme-labeled plate coated with an abrus precatorius toxin monoclonal antibody; and mixing and incubating to form an abrus precatorius toxin monoclonal antibody-abrus precatorius toxin-enzyme labeled antibody compound sandwich compound. The method disclosed by the invention has the characteristics of simplicity, rapidness, high sensitivity and the like, the detection range is 0-10 ng / mL, the lowest detection limit can reach 8.9 pg / mL, and the method is suitable for detecting the low-content abrus precatorius toxin of a large number of samples.

The invention relates to the technical field of toxin detection, in particular to a disc type full-automatic grain mycotoxin detector and a detection method thereof.The disc type full-automatic grain mycotoxin detector comprises a mixing pipe and a driving part, the power output end of the driving part is connected with the bottom of the mixing pipe, and a plurality of communicating mechanisms are arranged on the side wall of the mixing pipe in the circumferential direction; the liquid outlet ends of the communicating mechanisms are all connected with centrifugal tubes, and the communicating mechanisms enable the centrifugal tubes to communicate with the mixing tube under the action of rotating centrifugal force of the mixing tube. The detector adopts a centrifugal force and negative pressure suction mode to realize full-process automation from sample mixing, centrifugation to dilution and sample dropping detection, so that the detection efficiency is remarkably improved, and the detection error and the cross contamination risk are fundamentally eliminated.

The utility model relates to the technical field of specimen extraction, in particular to a specimen extraction device for food biotoxin detection, which comprises a food biotoxin specimen culture box, a plurality of through grooves are formed in the food biotoxin specimen culture box, and a same-column extraction structure is slidably mounted in each through groove. The same-column taking-out structure comprises sliding columns, each sliding column is partially exposed below the food biotoxin specimen culture box, the number of the sliding columns is five, a first connecting rod is fixedly installed between every two columns of sliding columns, and by arranging the same-column taking-out structure, a worker can lift up a handle on the side wall of a certain column of sliding columns to take out the food biotoxin specimen culture box; the handle generates a radial force to lift up the whole column of sliding columns through the handle rod, and the column of sliding columns slide upwards in the through groove of the food biotoxin specimen culture box, so that a worker can conveniently take out the specimen box subsequently; and the problem that the specimen boxes are inconvenient to take out by workers due to the fact that the specimen boxes are pushed together is solved.

In one embodiment, a fluorescence-based kit for detection and analysis of a toxin in a liquid sample includes a diluted antibody mixture in a preparation container to receive the liquid sample to produce a preparation solution; a fluorescent compound absorbed onto colloidal graphene in an analysis container to receive the preparation solution to produce an analysis solution; and a portable detector including one or more reference solutions each having a different one of a plurality of known levels of toxin concentration of the toxin, and a light source to shine a light onto the analysis solution and the one or more reference solutions.

The invention provides a kit for detecting endotoxin and a detection method thereof. The kit comprises: a capture unit: a recombinant crab-like hemocyanin factor C fixed on the surface of a solid phase carrier; the signal probe is composed of a polyclonal anti-lipopolysaccharide antibody coupled with gold nanoparticles and horse radish peroxidase; the reaction buffer solution is a Tris-HCl buffer solution containing calcium ions required by activation of Factor C and a surfactant; the signal substrate solution is a peroxidase chromogenic substrate solution containing 3, 3 ', 5, 5'-tetramethyl benzidine; the stop solution comprises a dilute sulphuric acid solution and an endotoxin standard substance. The rapid, high-sensitivity and high-specificity endotoxin detection kit based on the recombinant factor C and the nano signal amplification technology has the advantages of high specificity, interference resistance, high sensitivity, rapidness, convenience and the like, and is wide in application prospect.

The invention discloses a tachypleus amebocyte lysate based on a peptide-rhodamine 110 conjugate and application of the tachypleus amebocyte lysate in endotoxin detection. The tachypleus amebocyte lysate comprises a C factor, a B factor, a clotting zymogen and a peptide-R110 conjugate. The peptide-R110 conjugate is adopted as a limulus reagent substrate, when the limulus reagent substrate is applied to endotoxin detection, a fluorescence detection background signal can be remarkably reduced, the detection sensitivity is greatly improved, meanwhile, the dual detection functions of color development and fluorescence are achieved, end-point method reading can be achieved in a fluorescence mode, and the detection sensitivity is greatly improved. High-precision and high-reliability detection of trace endotoxin is realized, and the method has important significance in quality control in the pharmaceutical production process, safety evaluation of medical equipment and other fields needing endotoxin detection.

The application provides a pre-treatment kit and a pre-treatment method for endotoxin detection samples of biological products, and the pre-treatment kit for endotoxin detection samples of nuclease biological products is characterized in that the pre-treatment kit comprises a lysing agent A, a neutralizing agent B and a stabilizing agent C; the lysing agent A is a protein lysing enzyme, the neutralizing agent B is a hydrogen peroxide solution, and the stabilizing agent C is a magnesium sulfate solution. The pre-treatment kit and the pre-treatment method can be applied to the pre-treatment of various endotoxin detection methods such as a gel method, a chromogenic method and a recombinant C factor method, so that the recovery rate of various nuclease biological products reaches the standard.

The invention discloses a rapid marine product toxin detection method and system, and relates to the technical field of food safety detection.The rapid marine product toxin detection method comprises the steps that 5-10 g of a marine product sample is taken and homogenized through a portable handheld homogenizer, then 8-15 mL of a composite extracting solution is added, extraction is conducted for 8-12 min in a portable ultrasonic instrument at 28-38 DEG C, centrifugation is conducted for 3-4 min through a portable mini centrifugal machine, and the marine product toxin is obtained; taking supernate as to-be-detected liquid; the method comprises the following steps: preparing a test strip which takes a nitrocellulose membrane as a substrate and contains 3-5 parallel detection areas, and fixing aptamer-quantum dot conjugates corresponding to saxitoxin in each detection area; dropwise adding the to-be-detected liquid to a sample pad of the test strip, reacting for 4-6 minutes at 25-35 DEG C, and scanning the fluorescence intensity by using a portable fluorescence reading instrument; and the analysis module compares the threshold value output result within 15 seconds and synchronizes the result to the mobile terminal through Bluetooth. The method is completed within 30 minutes in the whole process, equipment is portable, multiple toxins can be synchronously detected on site, the screening efficiency is improved, and the food safety risk is reduced.

The invention provides a Bt Cry1Fa toxin detection monoclonal antibody and application, and belongs to the technical field of pesticide immunological detection. Amino acid sequences of a heavy chain variable region and a light chain variable region of the monoclonal antibody Cry1Fa-mAb are respectively shown as SEQ ID NO.3 and SEQ ID NO.4. The monoclonal antibody Cry1Fa-mAb can recognize Cry1Fa toxin with high sensitivity, can be used for high-sensitivity detection of Bt Cry1Fa toxin in food and environmental samples, such as DAS-ELISA detection, colloidal gold immunochromatography test paper detection (AuNPs-LFIA) or aggregation-induced emission immunochromatography test paper detection (AIEFMs-LFIA), and has wide application value.

The utility model discloses a be applicable to the powder sample of grain fungus toxin detection's short -fractionation sampling device, including casing, material box, short -fractionation assembly and sampling assembly, short -fractionation assembly is by short -fractionation board and installs fixed block on the lateral wall of short -fractionation board and is composed, the short -fractionation board divides a plurality of equal area short -fractionation unit, and the sampling assembly of different short -fractionation sampling ratio is formed through the different combination installation of blanking pipe and plugging piece. The utility model has the advantages that: short -fractionation assembly, sampling assembly and material box can be detachably installed in casing, through multiple inversion, simplify the operation of multiple short -fractionation sampling, and through the combination of plugging piece and material pipe, the short -fractionation ratio can be adjusted, the short -fractionation sampling ratio is adjusted, after determining the short -fractionation sampling ratio, the powder sample in material cavity is directly short -fractionated and sampled through equal division pipe, and the operation of short -fractionation sampling under different short -fractionation sampling ratios is simplified.