Immuomagnetic bead chromatographic test strip for rapidly detecting algae toxin and preparation method thereof

An immunomagnetic bead chromatography and detection test paper technology, which is applied in the directions of measuring devices, analytical materials, instruments, etc., can solve the problems such as the lack of commercial supply of specific protein phosphatase, poor specificity, and inability to meet the requirements of speed, convenience and accuracy. , to achieve the effect of cheap equipment, short response time and high sensitivity

Inactive Publication Date: 2009-10-07
JIANGNAN UNIV
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Problems solved by technology

Instrumental analysis methods mainly include high-performance liquid chromatography (HPLC), mass spectrometry, liquid chromatography-mass spectrometry, etc. Although these methods are sensitive, they require expensive instruments and equipment, professional operators, and relatively high requirements for inspection materials. Further sample pretreatment can be carried out, which can no longer meet the requirements of modern detection for fast, convenien

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  • Immuomagnetic bead chromatographic test strip for rapidly detecting algae toxin and preparation method thereof
  • Immuomagnetic bead chromatographic test strip for rapidly detecting algae toxin and preparation method thereof
  • Immuomagnetic bead chromatographic test strip for rapidly detecting algae toxin and preparation method thereof

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preparation example Construction

[0030] 2) Preparation and purification of polyclonal antibody: MC-LR-BSA was used as the immunogen, New Zealand white rabbits were used as the immunized animals, and the immune source was mixed with an equal amount of Freund's complete adjuvant to make an emulsifier for the first immunization , multi-point subcutaneous injection on the back of white rabbits, the immunization dose is 1 mg / rat, after an interval of 2-4 weeks, the same dose of immune source plus the same amount of Freund's incomplete adjuvant is mixed to make an emulsifier, strengthen the immunization, and monitor during the immunization Antibody titer and specificity, the last immunization without adjuvant. Seven days after the last immunization, the heart was bled, and the purified MC-LR polyclonal antibody was obtained by fractional precipitation with ammonium sulfate. And the antibody was purified by ammonium sulfate precipitation method; the IgG of rabbit anti-MC-LR was obtained.

[0031] 3) Preparation of ...

Embodiment 1

[0036] Weigh 40 mg of Taihu cyanobacteria, then use 1.5 mL of aqueous methanol (volume ratio 75:25) to ultrasonically extract for 10 minutes, then centrifuge at 10,000 rpm for 10 minutes to extract the supernatant. Repeat the extraction three times. The three supernatant extracts were mixed and filtered with a 0.2 μm microporous membrane, then blown dry with nitrogen, and finally dissolved the dry residue with a buffer solution as the sample solution to be tested. Finally, use the immunomagnetic bead chromatography test strip of algae toxin to detect the sample solution to be tested. The insertion depth does not exceed the marking line. The sample will flow through the test strip through capillary chromatography, and take out the test strip after 10 to 20 seconds. , after reacting at room temperature for 15 minutes, put the test strip into the Enviso TM The Magnetic Assay Reader of the System detects the magnetic field signal. Calculate the content of algal toxin in the sam...

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Abstract

The invention discloses an immuomagnetic bead chromatographic test strip for rapidly detecting algae toxin and a preparation method thereof, relating to the technical field of algae toxin detection. The test strip consists of a lining board as well as a sample pad, a magnetic scale combination pad, a coated film and an absorbing pad which are sequentially engaged on the lining board; the magnetic scale combination pad is glass fiber for absorbing algae toxin magnetic scale antibody; and the coated film is provided with an adelomorphic detection line T line which is printed by algae toxin-coupled carrier protein solution, and an adelomorphic control line C line which is printed by goat anti-rabbit IgG solution. The sample pad of the test strip is inserted into the solution of a tested sample, then taken out after 10-20 seconds, and then reacts for 15 minutes at room temperature; the test strip is arranged in a magnetic signal detector for detection, and the detector further outputs the biological responding signals which have been converted into magnetic field signals in the form of electrical signals; and after a specification curve is drawn, the algae toxin content in the tested sample is calculated according to the specification curve. The invention relates to the method based on nano-magnetic immunological technique for detecting algae toxin, and has the characteristics of high sensitivity, short reaction time, cheap instrument and equipment, convenience, easy usage and the like.

Description

technical field [0001] The invention discloses a nano-immune magnetic bead chromatography test strip for rapid detection of algal toxins and a preparation method thereof, relating to the technical field of algal toxin detection. Background technique [0002] With the development of the economy, sewage containing a large amount of nitrogen and phosphorus nutrients enters lakes and reservoirs, causing eutrophication of water bodies, causing abnormal proliferation of algae, releasing secondary metabolites algal toxins (Algae Toxins), threatening the safety of human drinking water. safety and the safety of other organisms in the water body. Among them, Microcystins (Microcystins, MCs) are widely distributed and more harmful. They are a family of cyclic heptapeptides with similar structures produced by certain species of freshwater cyanobacteria, mainly Microcystis aeruginosa. There are more than 60 isomers known. Its structure is as follows: [0003] [0004] It contains f...

Claims

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Application Information

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IPC IPC(8): G01N33/552G01N33/558G01N33/531G01N33/532
Inventor 胥传来李灼坤徐丽广刘丽强
Owner JIANGNAN UNIV
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