Primer probe combination for detecting methylation double sites of colorectal cancer and kit thereof

By combining primer and probe combinations for SDC2 and LIFR two-site detection and using molecular hybridization capture technology, the problem of low sensitivity in colorectal cancer screening has been solved, achieving high sensitivity and high specificity for early screening, which is suitable for early colorectal cancer screening of fecal samples.

CN116200493BActive Publication Date: 2026-06-02CHONGQING BOHAO DIAGNOSTIC TECH CO LTD +1
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Patent Information

Application Number
CN202211594794.X
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2022-12-13
Publication Date
2026-06-02
Estimated Expiration
2042-12-13

AI Technical Summary

Technical Problem

Existing colorectal cancer screening methods have low sensitivity, high false negative rates for single methylation site detection, and lack highly sensitive and specific combined detection methods.

Method used

A primer-probe combination for joint detection of SDC2 and LIFR sites was used, combined with molecular hybridization capture and multiplex fluorescent PCR technology, to detect the methylation sites cg08392199 of the LIFR gene and cg13096260 of the SDC2 gene in fecal samples. The target genes were captured by probe specificity and analyzed by real-time fluorescent PCR.

Benefits of technology

It achieves high sensitivity (92%) and high specificity (100%) in colorectal cancer detection, providing a high-sensitivity and high-specificity early screening method. It is fast, easy to operate, and low-cost, making it suitable for early colorectal cancer screening.

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Abstract

The application discloses a primer probe combination for detecting methylation of two sites of colorectal cancer and a kit thereof. The primer probe combination is a primer probe combination for detecting methylation of a cg08392199 site of a LIFR gene and a primer probe combination for detecting methylation of a cg13096260 site of a SDC2 gene; the methylation of the cg08392199 site of the LIFR gene and the cg13096260 site of the SDC2 gene is correlated with colorectal cancer. The kit comprises the primer probe combination. The application adopts combined detection of the two sites of SDC2 and LIFR for the first time, has innovation, and the sensitivity of combined detection of the two sites in the detection of colorectal cancer reaches 92%, and the specificity is as high as 100%, thereby providing a primer probe combination for early screening of colorectal cancer with high sensitivity and high specificity and a kit.
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