Aflatoxin M1 gold label quick detectiontest card and preparation method and application thereof

Abstract

The invention discloses an aflatoxin M1 gold label quick detection card and a preparation method and an application thereof, belongs to the field of immunology, and relates to a toxin detection technology. The detection card comprises a detection strip and a plastic card casing, wherein the detection strip is supported by a PVC (polyvinyl chloride) bottom lining and consists of a sample pad, a gold label antibody combination pad, an enveloping membrane and a water absorption pad which are sequentially connected; the gold label antibody combination pad is made of glass fibers, and envelops an aflatoxin M1 monoclonal antibody combined with colloidal gold particles; the enveloping membrane is a cellulose nitrate membrane and envelops a detection line (T line) containing recessive aflatoxin M1 protein conjugates and a control line (C line) containing a goat-anti-mouse monoclonal antibody; the sample pad is made of glass fibers processed by a buffering system. The detection card, based on the colloidal gold immunochromatograohic assay technology, is simple to operate, convenient to carry, and quick and accurate in result determination, requires only 40-50 minutes for detection, and is suitable for on-site supervision and qualitative screening of a great number of samples.

Description

technical field [0001] The invention belongs to the field of immunology, and relates to a product and a method for rapid detection of aflatoxin M1 in raw milk. Background technique [0002] Aflatoxin (Aflatoxin, AF) is a kind of aflatoxin, which is a derivative of dihydrofuranocoumarin. It is a highly carcinogenic mycotoxin widely present in food. Secondary metabolites of Asperillus parasiticus. Aflatoxin M1 (Aflatoxin M1, AFM 1 ) is an animal ingestion of aflatoxin B1 (Aflatoxin B1, AFB 1 ) after hydroxylation in the body, its hazards are mainly manifested in carcinogenicity and mutagenicity, and it has a destructive effect on human and animal liver tissue, which can lead to liver cancer and even death. It mainly exists in the edible parts of animals, such as Milk, liver, eggs, etc., among which milk is the most common. [0003] In animal experiments, the animals that successfully induce liver cancer include rats, mice, guinea pigs, snow leopards, ducks, dogs, cats, rab...

AI Technical Summary

Problems solved by technology

[0008] The technical problem to be solved by the present invention is to provide a kit capable of rapid screening and detection of aflatoxin M1 residues in raw milk samples and its detection method and application; the main purpose of the invention is to realize the rapid screening and detection of a large number of samples and solve the The method is time-consuming, high in cost, requires high professional requirements for operators, requires the assistance of instruments and equipment, and cannot realize the rapid screening of a large number of samples. At the same time, the detection of different detection sensitivities can be realized through sample pretreatment to meet the detection needs of different customers, thus providing A faster, simpler, more cost-effective and safer detection kit and detection method

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