A method for detecting zearalenone toxin in traditional Chinese medicine in different matrices

A technology of zearalenone and detection method, applied in the field of zearalenone toxin

Inactive Publication Date: 2011-12-28
INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Zearalenone toxin often exists in trace amounts, and conventional detection methods are difficult to be widely used due to the limitation of detection limit. Therefore, simplifying the detection steps and improving detection sensitivity and accuracy have become a major problem in the current detection of zearalenone toxin

Method used

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  • A method for detecting zearalenone toxin in traditional Chinese medicine in different matrices
  • A method for detecting zearalenone toxin in traditional Chinese medicine in different matrices
  • A method for detecting zearalenone toxin in traditional Chinese medicine in different matrices

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Experimental program
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Effect test

Embodiment 1

[0157] Embodiment one: (1), measure the content of zearalenone toxin in the traditional Chinese medicine with high performance liquid chromatography-fluorescence detection method;

[0158] a. Chromatographic conditions:

[0159] Use octadecylsilane bonded silica gel as filler, methanol-acetonitrile-water (8:46:46, V / V / V) as mobile phase, column temperature 25°C, excitation wavelength: 270nm, emission wavelength: 440nm;

[0160] b. Solution preparation:

[0161] Preparation of reference solution: Accurately weigh an appropriate amount of zearalenone standard substance, add acetonitrile to make a solution containing 40 μg / mL per ml;

[0162] Preparation of Tween-PBS buffer solution: Weigh 8.0g sodium chloride, 1.2g disodium hydrogen phosphate, 0.2g potassium dihydrogen phosphate, 0.2g potassium chloride and dissolve them in 900ml water, adjust the pH to 7.0 with concentrated hydrochloric acid, add 1ml Tween-20, dilute to 1000ml with water.

[0163] c. Preparation of sample so...

Embodiment 2

[0173] Embodiment two: (1), measure the content of zearalenone toxin in the traditional Chinese medicine with high performance liquid chromatography-fluorescence detection method;

[0174] a. Chromatographic conditions: use octadecylsilane bonded silica gel as filler, methanol-acetonitrile-water (6:46:48, V / V / V) as mobile phase, column temperature 27°C, excitation wavelength: 270nm, Emission wavelength: 440nm;

[0175] b. Solution preparation:

[0176] Preparation of reference solution: Accurately weigh an appropriate amount of zearalenone standard substance, add acetonitrile to make a solution containing 20 μg / mL per ml;

[0177] Preparation of Tween-PBS buffer solution: Weigh 8.0g sodium chloride, 1.2g disodium hydrogen phosphate, 0.2g potassium dihydrogen phosphate, 0.2g potassium chloride and dissolve them in 900ml water, adjust the pH to 7.0 with concentrated hydrochloric acid, add 1ml Tween-20, dilute to 1000ml with water.

[0178] c. Preparation of sample solution:

...

Embodiment 3

[0189] (1), determine the content of zearalenone toxin in the traditional Chinese medicine with high performance liquid chromatography-fluorescence detection method;

[0190] a. Chromatographic conditions: use octadecylsilane bonded silica gel as filler, methanol-acetonitrile-water (10:46:44, V / V / V) as mobile phase, column temperature 22°C, excitation wavelength: 270nm, Emission wavelength: 440nm;

[0191] b. Solution preparation:

[0192] Preparation of reference solution: Accurately weigh an appropriate amount of zearalenone standard substance, add acetonitrile to make a solution containing 60 μg / mL per ml;

[0193] Preparation of Tween-PBS buffer solution: Weigh 8.0g sodium chloride, 1.2g disodium hydrogen phosphate, 0.2g potassium dihydrogen phosphate, 0.2g potassium chloride and dissolve them in 900ml water, adjust the pH to 7.0 with concentrated hydrochloric acid, add 1ml Tween-20, dilute to 1000ml with water.

[0194] c. Preparation of sample solution:

[0195] Samp...

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Abstract

A method for detecting zearalenone toxin in traditional Chinese medicines in different matrices, including extraction, purification and detection of Chinese medicine samples, said samples are extracted homogeneously at high speed with methanol-water (80:20, V/V), pH7. 0 Tween-PBS buffer solution dilution, glass fiber membrane filtration, immunoaffinity column purification. The determination method includes: high performance liquid chromatography-fluorescence detection method, high performance liquid chromatography-diode array detection method determination, spectrum and high performance liquid chromatography-mass spectrometry confirmation. Liquid chromatography conditions are: mobile phase ratio methanol: acetonitrile: water (8:46:46, V/V/V), fluorescence detector wavelength: excitation wavelength: 270nm, emission wavelength: 440nm; diode array detector wavelength: 236nm . Liquid quality conditions: Methanol-0.1% ammonium acetate water (75:25, V/V) as mobile phase, atmospheric pressure chemical ionization positive ion mode, drying gas flow rate 6.00L/min, drying gas temperature 340°C, fragmentation voltage 150V, Full scan mode scan, scan range 200-400m/z. The invention has the characteristics of accuracy, precision, reliability and the like.

Description

Technical field: [0001] The invention relates to a method for determining zearalenone toxin in traditional Chinese medicines with different bases, in particular to high performance liquid chromatography-fluorescence detection method, high performance liquid chromatography-diode array detection method, spectrum and high performance liquid chromatography-mass spectrometry Method for confirmation of zearalenone toxin in traditional Chinese medicine by combination method. Background technique: [0002] Zearalenone toxin is an estrogenic mycotoxin produced by Fusarium graminearum, Fusarium three-line and Fusarium moniliforme, etc. It is widely distributed in nature and mainly exists in moldy corn, sorghum, wheat, oats, In cereal crops such as barley, as well as in dairy products, recent studies have found zearalenone toxin in the dried roots of ginseng and the middle leaves of medicinal plants such as the Sri Lankan medicinal plant Tribulus terrestris. Toxicology experiments hav...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N21/64G01N27/62
Inventor 杨美华张晓飞
Owner INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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