SPR sensor for detecting microcystic toxin based on nucleic acid aptamer signal amplification strategy and preparation method and application thereof

A technology of microcystin and nucleic acid aptamer, which is applied in the direction of instruments, measuring devices, scientific instruments, etc., can solve the problems of restricting the dynamics of microcystin pollution in a timely manner, restricting large-scale promotion and use, and expensive probes, etc. , to achieve the effects of easy construction of portable detection instruments for field monitoring, real-time analysis, and simple preparation methods

Active Publication Date: 2017-02-01
CHONGQING INST OF GREEN & INTELLIGENT TECH CHINESE ACADEMY OF SCI
View PDF5 Cites 13 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the enzymes used are expensive and easy to inactivate. In addition, the current sensor detection methods generally have problems such as low sensitivity, poor stability, and expensive probes, which limits the wide-scale promotion and use of this technology.
In recent years, molecular imprinting technology has the ability to spec

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • SPR sensor for detecting microcystic toxin based on nucleic acid aptamer signal amplification strategy and preparation method and application thereof
  • SPR sensor for detecting microcystic toxin based on nucleic acid aptamer signal amplification strategy and preparation method and application thereof
  • SPR sensor for detecting microcystic toxin based on nucleic acid aptamer signal amplification strategy and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A preparation method of an SPR sensor for detecting microcystins based on a nucleic acid aptamer signal amplification strategy. The specific steps of the preparation method are as follows:

[0032] (1) Preparation of glass chips: Clean the glass slides with a mixed solution of ammonia water: hydrogen peroxide: ultrapure water with a volume ratio of 1:1:1 for 1 hour, and then place them in ammonia with a volume fraction of 10% under anhydrous conditions. Propyltrimethoxysilane in ethanol solution for 10min, then wash with ultrapure water to make the surface positively charged amino groups, and finally immerse in citric acid stabilized gold nano solution for 2h to deposit gold nanoparticles on the treated surface through electrostatic force On the glass slide, a glass chip was obtained;

[0033] (2) DNA probe 1 modification of the glass chip: immerse the glass chip obtained in step (1) in a solution containing 0.03 μM DNA probe 1 for 1 h, so that the DNA probe 1 can be bo...

Embodiment 2

[0040] The method for detecting the content of microcystin in water by an SPR sensor for detecting microcystin based on a nucleic acid aptamer signal amplification strategy comprises the following steps:

[0041] (1) Carry out the preparation of sensor according to the method step of preparing SPR sensor in embodiment 1;

[0042] (2) The sensor in step (1) was immersed in the PBS buffer solution containing 0 μ M, 10 μ M, and 100 μ M microcystin respectively, and then the SPR signal change was measured, and the results were as follows: image 3 It can be seen from the figure that although the sensor contains different concentrations of microcystins before being washed with water, the response values ​​of the three groups increase at the same time because the injected DNA probe 2 modified noble metal nanoparticles stays on the surface. , but after washing with water, as the content of microcystin increases, the number of noble metal nanoparticles modified by DNA probe 2 bound to...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Thicknessaaaaaaaaaa
Particle sizeaaaaaaaaaa
Login to view more

Abstract

The invention belongs to the technical field of toxin detection and discloses an SPR sensor for detecting microcystic toxin based on a nucleic acid aptamer signal amplification strategy and a preparation method and application thereof. The SPR sensor comprises a glass chip, a microcystic toxin aptamer, a DNA probe 1, a DNA probe 2 and a precious metal nano material, wherein microcystic toxin detection can be performed by determining SPR signal change. The preparation of the SPR sensor is simple and facilitates establishment of a portable detecting instrument for field monitoring, and the cost is far lower than that of a large analysis and detection instrument.

Description

technical field [0001] The invention belongs to the technical field of toxin detection, and in particular relates to a surface plasmon (SPR) sensor for detecting microcystins based on a nucleic acid aptamer signal amplification strategy and a preparation method and application thereof. Background technique [0002] Microcystins (MCs) are a class of cyclic heptapeptide hepatotoxins released by harmful cyanobacteria blooms. They have strong carcinogenic effects and are important environmental factors that induce liver cancer, gastroenteritis and other diseases. In view of the toxicity and harm of microcystins, the World Health Organization (WHO) has listed microcystin-LR (MC-LR) as a harmful pollutant to be controlled in drinking water. The secondary pollution of microcystins caused by cyanobacteria blooms and its harm to human health have attracted people's attention, and it has become an environmental science problem faced by the whole world. [0003] At present, the analys...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N33/68G01N33/553
CPCG01N33/553G01N33/68G01N2333/405
Inventor 曹海燕黄昱魏东山张华汤冬云
Owner CHONGQING INST OF GREEN & INTELLIGENT TECH CHINESE ACADEMY OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap