Piezoelectric detection sensor for microcystic toxins based on molecular imprinting technology

A molecular imprinting technology, microcystin technology, applied in the direction of instruments, measuring devices, scientific instruments, etc., can solve the problems of inability to identify toxins, false positive reactions, etc. The effect of low requirements and simple operation

Active Publication Date: 2014-04-30
SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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Problems solved by technology

The disadvantage is that the toxin cannot be well identified, and false positive reactions will occur

Method used

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  • Piezoelectric detection sensor for microcystic toxins based on molecular imprinting technology
  • Piezoelectric detection sensor for microcystic toxins based on molecular imprinting technology
  • Piezoelectric detection sensor for microcystic toxins based on molecular imprinting technology

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specific Embodiment

[0032] For the synthesis of the microcystin molecularly imprinted membrane 3, the present invention adopts a photopolymerization synthesis method, and the quartz crystal oscillator is cleaned with an oxygen plasma cleaner for 2-4 minutes, and the ethanol solution of 50-1000 mM undecyl mercaptan or ten Soak in the ethanol solution of dithiol for more than 12 hours, take it out, rinse with ethanol, and blow dry with nitrogen; mix 0.1-200 umol microcystin and 1-2000 umol methacrylic acid in 1-2 mL ethanol solution and let stand for 3 hours Above, add 2-2000 umol ethylene glycol dimethacrylate and 0.016-16 mg benzophenone, and treat with nitrogen for more than 10 minutes; drop 100 uL of the prepared reaction solution on the surface of the sensor substrate, in a nitrogen protection environment Use 365±10 nm ultraviolet light to irradiate the photocrosslinking reaction. After the surface is slightly dry, continue to add the reaction solution dropwise, repeat more than 10 times, and t...

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Abstract

The invention discloses a piezoelectric detection sensor for microcystic toxins based on a molecular imprinting technology. The piezoelectric detection sensor is characterized in that a photochemistry polymerization method or an electrochemical polymerization method is utilized to compound a molecularly imprinted membrane, so that the combining capacity is improved, the delay time is shortened, and the specific adsorption capacity is strengthened; meanwhile, a quartz crystal serves as an effective apparatus of the sensor, so that the sensitivity of the sensor is improved, the stability is increased, and the cost is reduced. The piezoelectric detection sensor provided by the invention has the advantages that the property of the sensor is improved, the trace detection of the microcystic toxins is realized, and the microcystic toxins detection method which is high in sensitivity, high in speed, low in cost, and low in operation requirement is established.

Description

technical field [0001] The invention relates to the field of microcystin detection sensors, in particular to a microcystin piezoelectric detection sensor based on molecular imprinting technology. Background technique [0002] The middle and lower reaches of the Yangtze River is one of the areas where freshwater lakes are concentrated in my country. In the past few decades, human activities have had a great impact on the lake ecosystem in this area. The most prominent is the increasing eutrophication of suburban lakes. Lakes, reservoirs and rivers receive excessive nutrients such as nitrogen and phosphorus, which changes the ecological structure and function of the water body, resulting in the abnormal growth of algae, especially cyanobacteria, and the phenomenon of cyanobacteria blooms. The frequent occurrence of harmful algae blooms caused by the aggravation of eutrophication in water bodies has become an environmental problem of general concern. When the cyanobacteria blo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N5/00G01N33/569
Inventor 周连群何皓李传宇张志强董文飞王弼陡
Owner SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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