43results about How to "Improve specific recognition" patented technology

The invention belongs to the technical field of semiconductor devices and integrated systems and discloses a quantum dot-based electronic nose chip and a design method thereof. The design method includes the following steps that: (1) a gas sensor array on the electronic nose chip is designed; and (2) a signal processing unit and a micro control unit are designed. The step (1) further includes thefollowing steps that: (1-1) with a quantum dot material adopted as an olfactory acceptor material, a quantum dot gas sensor unit is designed, specifically, a resistive or field effect transistor typesensor unit on a substrate is designed; and (1-2) a quantum dot sensor array of an MEMS or TFT device structure is designed based on the quantum dot gas sensor unit. According to the quantum dot-basedelectronic nose chip and the design method thereof of the invention adopted, the quantum dot material is adopted as the olfactory receptor material; the micro-nano gas sensor is designed and prepared; the sensor array is obtained on the basis of the MEMS and TFT device structure; and the MCU containing a pattern recognition algorithm, and the signal processing unit are integrated on the chip, andtherefore, the quantum dot-based electronic nose chip which has the advantages of high sensitivity , small size and low power consumption can be obtained.

The invention discloses a rapid detecting sensor for microcystins. The rapid detecting sensor utilizes a photochemistry polymerization method or an electrochemical polymerization method to synthesize a molecularly imprinted membrane, so that the combining capacity is improved, the delay time is reduced, and the specific adsorption capacity is strengthened; meanwhile, a Lamb wave piezoelectric membrane is utilized as an effector of the sensor, so that the sensitivity of the sensor is improved, the stability is increased, the cost is reduced, the performance of the sensor is strengthened, the trace detection of the microcystins is realized, and thus the rapid detecting method for the microcystins, with high sensitivity, rapidness, low cost and low operation requirements, is established.

The invention provides sepecific nucleic acid aptamer. The nucleotide sequence of the specific nucleic acid aptamer is a sequence shown in the sequence table <400>1, the nucleic acid aptamer is screened through a whole bacterium reduction SELEX technique, is low in molecular weight and easy to synthesize and modify, can recognize enterohemorrhagic escherichia coli O157:H7 in a high-specific mode and be combined with the enterohemorrhagic escherichia coli O157:H7 in a high-affinity mode, does not specifically combined with other bacteria, can be used for detecting the enterohemorrhagic escherichia coli O157:H7 and has the important significant for effectively preventing and controlling infection of the enterohemorrhagic escherichia coli O157:H7.

The invention discloses a piezoelectric detection sensor for microcystic toxins based on a molecular imprinting technology. The piezoelectric detection sensor is characterized in that a photochemistry polymerization method or an electrochemical polymerization method is utilized to compound a molecularly imprinted membrane, so that the combining capacity is improved, the delay time is shortened, and the specific adsorption capacity is strengthened; meanwhile, a quartz crystal serves as an effective apparatus of the sensor, so that the sensitivity of the sensor is improved, the stability is increased, and the cost is reduced. The piezoelectric detection sensor provided by the invention has the advantages that the property of the sensor is improved, the trace detection of the microcystic toxins is realized, and the microcystic toxins detection method which is high in sensitivity, high in speed, low in cost, and low in operation requirement is established.

The invention discloses a non-target screening analysis method for intoxicating organic pollutants in wastewater based on a molecular network. The method comprises the following steps of: a) carryingout solid-phase extraction on pretreated wastewater to obtain an organic pollutant concentrated solution; b) fractionating the organic pollutant concentrated solution by using a high performance liquid chromatograph equipped with an automatic fraction collector to obtain a to-be-detected fraction; c) analyzing and detecting the to-be-detected fraction in the step b) by adopting a high performanceliquid chromatography-time-of-flight mass spectrometer; and d) processing the mass spectrum data detected in the step c) by using open source analysis software, generating a molecular network associated with the organic pollutants, and analyzing substances of network nodes. According to the non-target screening analysis method for the intoxicating organic pollutants in the wastewater based on themolecular network, the pollutant molecular network is generated, the network nodes display the response intensity of the substance in different components in a pie chart form, and the concentrations of the same substance in different components can be visually compared.

The invention discloses a dual-signal-amplification fluorescence sensor for detecting mercury ions and a preparation method thereof. The fluorescence sensor comprises seven probes, namely a, b, c andd, namely a probe S, a probe F, a probe F1, a probe F2, a probe IP, a probe H1 and a probe H2; fuel stimulation isothermal amplification (FSP) primary circulation is realized based on specific recognition (T- Hg<2+> -T specific base mismatch) of the mercury ions and nucleic acid, an F2 chain released by the FSP can achieve chain displacement catalytic card assembly reaction (CHA) with H1 and H2, secondary circulation is achieved, and lastly the mercury ions are detected through fluorescence signal enhancement. The fluorescence sensor is advantaged in that the sensor has low detection limit, high sensitivity, high reaction speed, strong anti-interference capability, mild reaction conditions and the like, can make up for defects of a mercury ion detection method in the prior art, and realizes rapid and accurate quantitative detection of the mercury ions.

The invention provides a compound, which is obtained by splicing a heptamethine cyanine structure and an epidermal growth factor receptor(EGFR) targeted drug molecular structure. The compound providedby the invention can be used as a hypoxia and EGFR double-targeted near infrared fluorescence probe, can realize the purpose of accurate imaging on malignant tumor lesions with high EGFR expression by targeting the tumor marker EGFR and the hypoxia state of tumor cells; in addition, the compound provided by the invention can be used as a photosensitizer for cancer treatment.

The invention discloses a gold-magnetic immunochromatographic test strip for rapid quantitative detection of lean meat powder (clenbuterol hydrochloride and ractopamine) in pork. The test strip comprises a base plate, a sample pad, a conjugate release pad, a porous filter membrane and an absorption pad, wherein the sample pad, the conjugate release pad, the porous filter membrane and the absorption pad adhere to the base plate and are connected in sequence. The porous filter membrane is coated with a detection line T1 for a clenbuterol hydrochloride-BSA coupled antigen, a detection line T2 for a ractopamine-BSA coupled antigen and a quality control line C coated with a goat-anti-mouse secondary antibody in sequence, and a monoclonal antibody, marked with gold-magnetic nanoparticles, for clenbuterol hydrochloride and ractopamine is embedded in the sample pad. The test strip combines the advantages of color development and bio-affinity of a colloidal gold test strip, and the advantages of nano-magnetic beads that separation is easy and quantitative detection can be achieved; by means of the prepared test strip, qualitative detection can be conducted through visual inspection, and quantitative detection can also be conducted through magnetic measurement.

The invention discloses a preparation method of a carbon dot fluorescent probe. The method comprises the following steps: stirring and dispersing an umbelliferone derivative and amino acids in an aqueous solution, performing a reaction at 180-200 DEG C for 12-15 h, centrifuging the obtained suspension to remove large particles, filtering the obtained supernatant by a 0.22-0.25 [mu]m filter membrane to thoroughly remove the large particles, and dialyzing and freeze-drying the filtered supernatant to obtain the product. The probe can realize high-sensitivity and high-selectivity detection of Fe<3+>. The synthesis strategy of the carbon dot hybrid material is provided, a new way is opened up for ion detection, and high-selectivity and specific recognition of iron ions in a water phase is realized.

The invention discloses a set of HDACs nucleic acid aptamers, and belongs to the technical field of genetic engineering, immunology and oncology. The aptamers are any one or several nucleotide sequences as shown in SEQ ID NO: 1-5. The nucleic acid aptamers of the invention are obtained by screening with a protein SELEX technology, have a small molecular weight, are easy to synthesize and modify, and can recognize HDACs with high specificity and bind with the HDACs with high affinity. The aptamers do not bind with other protein, have no immunogenicity, are stable and easy to modify, and are easy to synthesize and preserve. An HDACs detection kit and reagent papers prepared from the aptamers can detect the HDACs with high sensitivity, and are easy to optimize. The aptamers are used in tumordiagnosis, evaluation of tumor prognosis and monitoring, and tumor diagnosis.

The invention discloses a fluorescent molecularly imprinted polymer based on glycopeptide, a preparation method and application in glycoprotein screening and detection. The fluorescent molecularly imprinted polymer is prepared by the following steps of dispersing short glycopeptide immobilized N-GQDs@SiO2@FPBA of OVA in a phosphate buffer solution, adding MAA and MBA, performing ultrasonic dissolution, pre-assembling at 28-32 DEG C for 11-13 hours, then adding TEMED and APS, reacting at 28-32 DEG C for 5-7 hours under the protection of inert gas, eluting, and acquiring the fluorescent molecularly imprinted polymer based on the glycopeptide. Compared with a traditional glycoprotein detection method, the fluorescent molecularly imprinted polymer based on glycopeptide has the advantages of high specific recognition and sensitive and rapid detection, and screening and detection of trace target glycoprotein from a biological sample are very significant.

The invention relates to the technical field of the dual-photon fluorescence probe, and specifically relates to an application of the dual-photon fluorescence probe. The dual-photon fluorescence probeis applied to the cell and tissue imaging and used for detecting lipid raft content and distribution dynamic; the probe can improve the accuracy, precision, sensitivity and imaging resolution of thelipid raft detection, thereby providing a new thought for further preventing and controlling Alzheimer's disease and Prion Disease and like neurodegenerative diseases.