43results about How to "Improve specific recognition" patented technology

The invention provides a compound, which is obtained by splicing a heptamethine cyanine structure and an epidermal growth factor receptor(EGFR) targeted drug molecular structure. The compound providedby the invention can be used as a hypoxia and EGFR double-targeted near infrared fluorescence probe, can realize the purpose of accurate imaging on malignant tumor lesions with high EGFR expression by targeting the tumor marker EGFR and the hypoxia state of tumor cells; in addition, the compound provided by the invention can be used as a photosensitizer for cancer treatment.

The invention discloses a colorimetric probe for identifying iron ions and its preparation and application. The colorimetric probe has a structure shown in formula I: the colorimetric probe of the invention is prepared by one-step synthesis, and the synthesis conditions The method is simple, easy to purify and high in yield; the prepared colorimetric probe has a strong ability to identify iron ions and a fast response speed, and has practical application value in the fields of biochemistry, environmental science and the like.

The present invention discloses a preparation method of bisphenol A-coated antigen with polylysine as a carrier and application thereof, is aimed at providing the preparation method of bisphenol A-coated antigen with polylysine as the carrier which is simple in the synthetic method and the application thereof, and finally establishes an enzyme linked immunosorbent assay kit. The kit has the advantages of simple pre-treatment, fast speed and accuracy and low cost, and can be used for on-site testing in large scale. The technical points include: taking polylysine as a carrier protein, preparing bisphenol A-coated antigen by coupling with diphenolic acid, and applying the bisphenol A-coated antigen to prepare the bisphenol A enzyme linked immunosorbent assay kit, wherein the bisphenol A enzyme linked immunosorbent assay kit is composed of a solid phase carrier of envelope antigen, an antibody working solution, a bisphenol A standard solution, an enzyme labeled secondary antibody solution, an antibody diluent, a wash concentrated solution, a substrate solution and a stop solution. The preparation method belongs to the technical field of enzyme linked immunoassay.

The invention relates to the technical field of food toxin detection, in particular to a colorimetric sensor, detection system and application for the detection of mycotoxins in food. The colorimetric sensor includes an aptamer sequence, and also includes nanomaterials that modify HS-DNA, and the HS-DNA sequence is a sequence that is complementary to the aptamer sequence and has mismatched bases in the middle. The colorimetric sensor has the advantages of enzyme-free, low cost, simple preparation, rapid on-site visualization, and high sensitivity, and can realize on-site rapid and visual detection of mycotoxins in liquid food.

The invention discloses a functional detection paper and its preparation method and application. The structure of the functional detection paper contains high-density amino groups, carboxyl groups, and sulfur-containing groups, and through the synergistic effect of the three groups, the detection of Ag + 、Cu 2+ rapid and high-sensitivity detection; the functional detection paper is based on polyacrylonitrile fiber, which is aminated with amine reagents to obtain aminated polyacrylonitrile fiber, and then combined with two kinds of sulfur-containing polyacrylonitrile fibers of type A and type B Carboxylic acid small-molecule reagents are subjected to amidation reaction to prepare polyacrylonitrile-based functional fibers, and finally polyacrylonitrile-based functional fibers are mixed with bleached pulp fibers and reinforcing agents to form paper, and functional detection paper is prepared. The function detection paper of the present invention is simple and portable, has stable performance and low cost, and can realize the detection of Ag + 、Cu 2+ Rapid and highly sensitive detection of Ag + 、Cu 2+ The detection sensitivity can reach 10 ‑7 mol / L, the detection response time is 10-120s.

The present invention relates to a dengue fever NS1 antigen and IgG / IgM antibody dual detection test strip, the test strip includes a dengue fever NS1 antigen test strip and a dengue fever IgG / IgM antibody test strip, and the dengue fever NS1 antigen test strip includes the first gold label Cushion layer and the first nitrocellulose membrane, the first gold standard cushion layer is immobilized with dengue fever NS1 monoclonal antibody-colloidal gold complex, and the first nitrocellulose membrane is coated with dengue fever NS1 monoclonal antibody of four serotypes The detection line of dengue fever IgG / IgM antibody test strips includes the second gold standard cushion layer and the second nitrocellulose membrane, and the second gold standard cushion layer is solid-phased with four serotypes of dengue fever recombinant antigen-colloidal gold complex , the second nitrocellulose membrane is coated with the detection line T1 of mouse anti-human IgG monoclonal antibody and the detection line T2 of mouse anti-human IgM monoclonal antibody; the test strip of the present invention can jointly detect IgM / IgG antibody, NS1 antigen simplifies the operation process, effectively diagnoses the state of the body's immune response, and achieves the purpose of real POCT detection.

The invention discloses a bifunctional probe for recognizing iron ions and fluorine ions and its preparation and application. The rate is high; it can realize the detection of iron ion and fluorine ion, and the recognition of fluorine ion is also reversible.

The invention discloses a stilbene estrogen nucleic acid aptamer, including at least one of Apt-7, Apt-21 and Apt-31, wherein the sequence of Apt-7 is shown in SEQ ID NO.01, Apt-7 The sequence of 21 is shown in SEQ ID NO.02, and the sequence of Apt-31 is shown in SEQ ID NO.03. The nucleic acid aptamer of the present invention can recognize and bind stilbene estrogens with high specificity and high affinity, and can be applied to related methods for detecting stilbene estrogens, in terms of detection of stilbene estrogens and It is of great significance in reducing the damage of stilbene estrogens to the human body.

The present invention relates to the technical field of two-photon fluorescent probes, in particular, to the use of a two-photon fluorescent probe, which is used in cell and tissue imaging to detect lipid raft content and distribution dynamics. The probe can improve the accuracy, precision, sensitivity, and imaging resolution of lipid raft detection, and provide new ideas for further prevention and control of neurodegenerative diseases such as Alzheimer's disease and prion disease.

The invention provides a method for detecting salmonellae. The method comprises the following steps: synthesizing a DNA silver nano cluster; and carrying out index enlargement under the combined action of Phi 29 polymerase and Nt.AlwI endonuclease and homogeneous reaction of strand displacement cycle amplification, and then carrying out fluorescence detection. The method provided by the invention has the advantages that specific recognition of an aptamer is utilized, and the aptamer is designed into a hairpin structure, so that high specificity detection on a target object salmonellae is realized; and cyclic utilization of Trigger is realized by utilizing the combined action of the Phi 29 polymerase and Nt.AlwI endonuclease as well as strand displacement reaction, signal simplifying effect is achieved, and detection sensitivity is effectively improved.

The invention discloses an organochlorine pesticide DDT nucleic acid aptamer, including at least one of DDT-01 to 17, wherein the DDT-01 to 17 have sequences as shown in SEQ ID NO. 01 to 17 in sequence. The nucleic acid aptamer of the present invention can recognize DDT with high specificity and bind with high affinity, and can be applied to related methods for detecting DDT of organochlorine pesticides, in the detection of DDT residues in animal bodies and water bodies, and in reducing the effect of DDT on human body. is of great significance in terms of violations.