Rapid detection of lean meat powder in pork

A pork and detection area technology is applied in the field of rapid detection of gold magnetic nano-immunochromatographic test strips, which can solve the problems of missed detection and low sensitivity, and achieve the effects of simple operation, high affinity and adsorption force, and low price.

Inactive Publication Date: 2017-03-22
陈军 +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, because it can only be used for qualitative or semi-quantitative detection, it is difficult to meet the quantitative requirements of detection indicators. At the same time, the detection results are judged by naked eyes, especially when the detection results are weakly positive, which can easily cause artificial missed detection. Issues such as low sensitivity need to be further improved

Method used

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  • Rapid detection of lean meat powder in pork
  • Rapid detection of lean meat powder in pork
  • Rapid detection of lean meat powder in pork

Examples

Experimental program
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Embodiment

[0069] see figure 1 and figure 2 As shown, the gold magnetic immunochromatographic test strip for rapid determination of clenbuterol includes a support pad (bottom plate) 11 and a sample pad 14 attached to the bottom plate in sequence, a binding release pad 13, a porous filter membrane 10 and an absorption pad. Pad 12, detection T1 line 20 coated with clenbuterol hydrochloride-BSA conjugated antigen, detection T2 line 20* coated with ractopamine-BSA conjugated antigen, and goat anti-mouse secondary antibody coated sequentially on the porous filter membrane The quality control C line 21, the sample conjugate release pad 13 is embedded with monoclonal antibodies of clenbuterol hydrochloride and ractopamine labeled with gold magnetic nanoparticles.

[0070] Wherein the monoclonal antibody preparation steps of clenbuterol hydrochloride or ractopamine labeled with gold magnetic nanoparticles on the conjugate release pad are as follows:

[0071] (1) Preparation of gold magnetic n...

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Abstract

The invention discloses a gold-magnetic immunochromatographic test strip for rapid quantitative detection of lean meat powder (clenbuterol hydrochloride and ractopamine) in pork. The test strip comprises a base plate, a sample pad, a conjugate release pad, a porous filter membrane and an absorption pad, wherein the sample pad, the conjugate release pad, the porous filter membrane and the absorption pad adhere to the base plate and are connected in sequence. The porous filter membrane is coated with a detection line T1 for a clenbuterol hydrochloride-BSA coupled antigen, a detection line T2 for a ractopamine-BSA coupled antigen and a quality control line C coated with a goat-anti-mouse secondary antibody in sequence, and a monoclonal antibody, marked with gold-magnetic nanoparticles, for clenbuterol hydrochloride and ractopamine is embedded in the sample pad. The test strip combines the advantages of color development and bio-affinity of a colloidal gold test strip, and the advantages of nano-magnetic beads that separation is easy and quantitative detection can be achieved; by means of the prepared test strip, qualitative detection can be conducted through visual inspection, and quantitative detection can also be conducted through magnetic measurement.

Description

technical field [0001] The invention belongs to the field of biological immunological detection, and in particular relates to the preparation and application of a gold magnetic nano-immunochromatographic test strip for the rapid detection of clenbuterol hydrochloride and ractopamine in meat products. Background technique [0002] Immunochromatography, which appeared in the early 1980s, is a new type of detection technology that combines immunolabeling technology with chromatography technology. It uses antigen-antibody specific reactions to form immune complexes, and then quantitatively detects analytes. A highly selective analysis method, the immunoassay method can realize the detection of trace substances such as drugs, microorganisms, protein substances, cytokines and nucleic acids. Compared with other detection techniques, it has the characteristics of: fast, simple and cheap, It has strong specificity and good stability, and can meet the characteristics of high-throughpu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543
CPCG01N33/54326
Inventor 陈军
Owner 陈军
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