Dengue fever ns1 antigen, IgG/IGM antibody dual detection test strips, detection cards and kits

Abstract

The present invention relates to a dengue fever NS1 antigen and IgG / IgM antibody dual detection test strip, the test strip includes a dengue fever NS1 antigen test strip and a dengue fever IgG / IgM antibody test strip, and the dengue fever NS1 antigen test strip includes the first gold label Cushion layer and the first nitrocellulose membrane, the first gold standard cushion layer is immobilized with dengue fever NS1 monoclonal antibody-colloidal gold complex, and the first nitrocellulose membrane is coated with dengue fever NS1 monoclonal antibody of four serotypes The detection line of dengue fever IgG / IgM antibody test strips includes the second gold standard cushion layer and the second nitrocellulose membrane, and the second gold standard cushion layer is solid-phased with four serotypes of dengue fever recombinant antigen-colloidal gold complex , the second nitrocellulose membrane is coated with the detection line T1 of mouse anti-human IgG monoclonal antibody and the detection line T2 of mouse anti-human IgM monoclonal antibody; the test strip of the present invention can jointly detect IgM / IgG antibody, NS1 antigen simplifies the operation process, effectively diagnoses the state of the body's immune response, and achieves the purpose of real POCT detection.

Description

technical field [0001] The invention belongs to the field of biological detection, and in particular relates to a double detection test strip, a detection card and a kit for detecting dengue virus IgG / IgM antibodies and NS1 antigens in serum, plasma and whole blood by using immunochromatography technology. Background technique [0002] Dengue fever is caused by dengue virus and is an acute infectious disease transmitted by Aedes aegypti or Aedes albopictus. Dengue virus is a small flavivirus, which belongs to the Flavivirus genus (Flavivirus) of the family Flaviviridae. According to the epidemiological classification, it belongs to group B of arboviruses and is divided into four serotypes serologically. Dengue virus can cause a series of clinical symptoms. Clinically, the main symptoms are sudden high fever, extensive hemorrhage, severe muscle pain and arthralgia, and extreme fatigue, often accompanied by rash, lymphadenopathy, and leukopenia. [0003] At present, the virus...

AI Technical Summary

Problems solved by technology

ELISA method and PCR method, these detection techniques require special instruments and equipment, and require professional technicians to conduct detection in professional laboratories, and the operation is cumbersome, technically difficult, time-consuming, experimental conditions are high, and there are serious biosafety It is not suitable for real-time on-site detection and is suitable as a confirmation method, but it cannot be used for large-scale primary screening and should be popularized and applied in actual work.