Carbon dot fluorescent probe, and preparation method and application thereof

A carbon dot fluorescence and probe technology, which is applied in the field of organic small molecule fluorescent probes, can solve the problems of not being able to prevent iron ion corrosion well, and the experience cycle cannot be well matched, and achieve the effect of high selectivity detection

Pending Publication Date: 2020-04-10
THE SECOND RES INST OF CIVIL AVIATION ADMINISTRATION OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the transportation situation is affected by many conditions, and the experience cycle does not match the actual situation well, and the corrosion is often more serious than expected, and it cannot prevent iron ion corrosion well

Method used

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  • Carbon dot fluorescent probe, and preparation method and application thereof
  • Carbon dot fluorescent probe, and preparation method and application thereof
  • Carbon dot fluorescent probe, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Disperse 0.25 mmol of 4-methylumbelliferone in a solution containing 10 mL of ultrapure water and 0.25 mmol of D-arginine under gentle stirring. The mixture was transferred into a 50ml stainless steel autoclave lined with polytetrafluoroethylene, and the temperature of the autoclave was heated to 180°C and maintained for 12h. After naturally cooling to room temperature, the resulting suspension was centrifuged at a speed of 10,000 rpm for 5min to remove Large particles, and then the supernatant is filtered through a 0.22μm water membrane to further remove large particles completely. Finally, the crude solution was diluted with 10 ml of ultrapure water, and dialyzed in a cellulose ester film bag (molecular weight cut-off 200 Da) for 2 hours to obtain a purified product. After lyophilization, the yellow-brown powder was collected and redissolved in ultrapure water as a stock solution at a concentration of 0.5 mg / mL (called CDs-4-MU).

Embodiment 2

[0022] 0.5 mmol of 4-methylumbelliferone was dispersed under gentle stirring in a solution containing 20 mL of ultrapure water and 0.5 mmol of D-arginine. The mixture was transferred into a 100ml stainless steel autoclave lined with polytetrafluoroethylene, and the temperature of the autoclave was heated to 190°C and kept for 14h. After naturally cooling to room temperature, the resulting suspension was centrifuged at a speed of 20,000 rpm for 5min to remove Large particles, and then the supernatant is filtered through a 0.22μm water membrane to further remove large particles completely. Finally, the crude solution was diluted with 20 ml of ultrapure water, and dialyzed with stirring in a cellulose ester film bag (molecular weight cut-off 200 Da) for 2 hours to obtain a purified product. After lyophilization, the yellow-brown powder was collected and redissolved in ultrapure water as a stock solution at a concentration of 0.5 mg / mL (called CDs-4-MU).

Embodiment 3

[0024] 1 mmol of 4-methylumbelliferone was dispersed under gentle stirring in a solution containing 40 mL of ultrapure water and 1 mmol of D-arginine. The mixture was moved into a 200ml stainless steel autoclave lined with polytetrafluoroethylene, the temperature of the autoclave was heated to 200°C and kept for 15h, after cooling to room temperature naturally, the resulting suspension was centrifuged at a speed of 15000 rpm for 5min, Remove large particles, and then the supernatant is filtered through a 0.22 μm water membrane to further completely remove large particles. Finally, the crude solution was diluted with 40 ml of ultrapure water, and dialyzed with stirring in a cellulose ester film bag (molecular weight cut-off 200 Da) for 2 hours to obtain a purified product. After lyophilization, the yellow-brown powder was collected and redissolved in ultrapure water as a stock solution at a concentration of 0.5 mg / mL (called CDs-4-MU).

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Abstract

The invention discloses a preparation method of a carbon dot fluorescent probe. The method comprises the following steps: stirring and dispersing an umbelliferone derivative and amino acids in an aqueous solution, performing a reaction at 180-200 DEG C for 12-15 h, centrifuging the obtained suspension to remove large particles, filtering the obtained supernatant by a 0.22-0.25 [mu]m filter membrane to thoroughly remove the large particles, and dialyzing and freeze-drying the filtered supernatant to obtain the product. The probe can realize high-sensitivity and high-selectivity detection of Fe<3+>. The synthesis strategy of the carbon dot hybrid material is provided, a new way is opened up for ion detection, and high-selectivity and specific recognition of iron ions in a water phase is realized.

Description

technical field [0001] The invention belongs to the field of organic small molecule fluorescent probes, and relates to a preparation method of a carbon dot fluorescent probe and its products and applications. [0002] technical background [0003] Iron elements are inevitably introduced during the transportation and storage of aviation fuel. In the presence of trace amounts of water, iron elements are ionized into iron ions, causing irreversible slow corrosion to pipes and containers. The presence of iron ions cannot be observed from the appearance, and it is impossible to intuitively judge when to add the corrosion inhibitor again after adding the corrosion inhibitor. The empirical cycle of adding the corrosion inhibitor can only be obtained through repeated sampling tests and data analysis. However, the transportation situation is affected by many conditions, and the experience cycle does not match the actual situation well, and the corrosion is often more serious than expe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/02C09K11/65G01N21/64
CPCC09K11/65C09K11/025G01N21/643G01N2021/6432
Inventor 叶李薇彭华乔李林刘曦非张亚博王强张帆王天明苏正良
Owner THE SECOND RES INST OF CIVIL AVIATION ADMINISTRATION OF CHINA
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