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A colorimetric sensor, detection system and application for the detection of mycotoxins in food

A colorimetric sensor and mycotoxin technology, applied in the field of colorimetric sensor and mycotoxin detection in food, can solve the problems of long reaction time, complicated preparation process, increased detection cost, etc., and achieve weak hydrogen bond force and obvious color change. , the effect of fewer mismatched bases

Active Publication Date: 2022-04-26
OCEAN UNIV OF CHINA
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Problems solved by technology

But there are the following disadvantages: 1. The mixed solution composed of DNA-ALP-MB and various concentrations of OTA was incubated at 25°C for 30min; after magnetic separation, it was mixed with AAP and incubated at 37°C for 30min; then, AuNRs solution was added and AgNO 3 The mixture and DEA buffer were incubated at 25°C for 10 minutes; the reaction time was long, and it was difficult to meet the purpose of rapid on-site visual detection
2. Various nanomaterials such as DNA alkaline phosphatase, magnetic beads, and nano-gold rods are used, the preparation process is complicated, and the detection cost increases
However, the distance between AuNPs is close enough and the ends can form a fully complementary double-stranded DNA structure to produce color changes. Therefore, the length of the surface-modified DNA is required, which limits its application, and it is difficult to combine with aptamers with longer sequences. Combining and simultaneously exerting the ability of rapid visualization of non-crosslinked aggregates and high specific recognition of aptamers

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[0050] In order to clearly illustrate the technical characteristics of the present solution, the present invention will be described in detail below through specific implementation modes and in conjunction with the accompanying drawings.

[0051] The OTA aptamer sequence and HS-DNA sequence used in the experiment of the present invention were all synthesized by Sangon Bioengineering (Shanghai) Co., Ltd. The specific sequence information is shown in Table 1.

[0052] Ochratoxin A, ochratoxin B, aflatoxin B1, aflatoxin B2, and deoxynivalenol were purchased from Qingdao Prebang Bioengineering Co., Ltd.

[0053] The other chemical reagents are Sinopharm chemical reagents, which can be used without further purification.

[0054] Table 1 Nucleotide sequence

[0055]

[0056] The colorimetric sensor for the detection of mycotoxins in food of the present invention is specifically a colorimetric sensor for OTA detection in food, including OTA aptamer sequences and AuNPs modified with...

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Abstract

The invention relates to the technical field of food toxin detection, in particular to a colorimetric sensor, detection system and application for the detection of mycotoxins in food. The colorimetric sensor includes an aptamer sequence, and also includes nanomaterials that modify HS-DNA, and the HS-DNA sequence is a sequence that is complementary to the aptamer sequence and has mismatched bases in the middle. The colorimetric sensor has the advantages of enzyme-free, low cost, simple preparation, rapid on-site visualization, and high sensitivity, and can realize on-site rapid and visual detection of mycotoxins in liquid food.

Description

technical field [0001] The invention relates to the technical field of food toxin detection, in particular to a colorimetric sensor for the detection of mycotoxins in food, a detection system and an application thereof. Background technique [0002] Ochratoxin A (OTA) is a toxic secondary metabolite mainly derived from Aspergillus and Penicillium. In our daily life, it is widely found in various foods and beverages, such as wheat, wine and fruit juice. It is considered nephrotoxic, hepatotoxic, neurotoxic, teratogenic, carcinogenic and immunotoxic in humans. Therefore, the International Agency for Research on Cancer (IARC) classified ochratoxin A as a human carcinogen of category 2B in 1993. In addition, OTA also has strong chemical stability. Once it enters the human body and accumulates, it will seriously affect human health. [0003] Chromatographic methods, such as high performance liquid chromatography (HPLC), liquid chromatography (LC), fluorescence detection aided ...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/53G01N21/33
CPCG01N33/5308G01N21/33
Inventor 王国庆丁善森王璐阳何芷毓李钰金田永帅韩肖王瑞华
Owner OCEAN UNIV OF CHINA
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