Optical detection sensor for microcystin

A microcystin and optical detection technology, which is applied in the measurement of color/spectral characteristics and phase influence characteristics, can solve the problems of inability to identify toxins and false positive reactions, and achieve improved sensitivity, low requirements, and Simple operation effect

Active Publication Date: 2015-02-11
SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage is that the toxin cannot be well identified, and false positive reactions will occur

Method used

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  • Optical detection sensor for microcystin
  • Optical detection sensor for microcystin

Examples

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Effect test

Embodiment 1

[0024] Modified on the surface of the gold layer by thermal polymerization microcystin molecularly imprinted membrane: clean the gold-plated glass sheet with an oxygen plasma cleaner for 2 minutes, soak it in an ethanol solution of 50mM undecylthiolanic acid for more than 12 hours, take it out Rinse with ethanol, blow dry with nitrogen; then immerse in a mixed aqueous solution of 0.1M 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and 0.01M N-hydroxysuccinimide 1 hour; take it out and immerse it directly in 100mM aminobutyramide hydrochloride aqueous solution for 3 hours, blow dry with nitrogen; take 0.1umol microcystin, 1umol methacrylic acid and 2umol trimethylolpropane trimethacrylate and add 5mL dimethicone Mix the methyl sulfoxide solution, treat with nitrogen for 10 minutes, immerse the pretreated glass sheet, and conduct thermal polymerization reaction at 65±5°C for 18 hours after sealing; after taking it out, use a mixed solution of ethanol and acetic acid ...

Embodiment 2

[0026] Modified on the surface of the gold layer by thermal polymerization microcystin molecularly imprinted membrane: clean the gold-plated glass sheet with an oxygen plasma cleaner for 4 minutes, soak it in an ethanol solution of 1000mM undecaptanic acid for more than 12 hours, and take it out Rinse with ethanol, blow dry with nitrogen; then immerse in a mixed aqueous solution of 1M 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and 0.1M N-hydroxysuccinimide 1 After taking it out, immerse it directly in 500mM aminobutyramide hydrochloride aqueous solution for 3 hours, blow dry with nitrogen; take 200umol microcystin, 2000umol methacrylic acid and 2000umol trimethylolpropane trimethacrylate and add 10mL dimethyl Mix the sulfoxide solution, treat with nitrogen for 12 minutes, immerse in the pretreated glass sheet, and conduct thermal polymerization reaction at 65±5°C for 15 hours after sealing; after taking it out, use a mixed solution of ethanol and acetic acid in...

Embodiment 3

[0028]Modified on the surface of the gold layer by thermal polymerization microcystin molecularly imprinted membrane: clean the gold-plated glass sheet with an oxygen plasma cleaner for 3 minutes, soak it in an ethanol solution of 200mM undecaptanic acid for more than 12 hours, take it out Rinse with ethanol, blow dry with nitrogen; then immerse in a mixed aqueous solution of 0.2M 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and 0.05M N-hydroxysuccinimide 1 hour; take it out and immerse it directly in 200mM aminobutyramide hydrochloride aqueous solution for 3 hours, blow dry with nitrogen; take 100umol microcystin, 200umol methacrylic acid and 300umol trimethylolpropane trimethacrylate and add 6mL dimethyl Mix the base sulfoxide solution, treat with nitrogen for 13 minutes, immerse in the pretreated glass sheet, and conduct thermal polymerization reaction at 65±5°C for 20 hours after sealing; after taking it out, use a mixed solution of ethanol and acetic acid in...

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Abstract

The invention discloses an optical detection sensor for microcystin. The optical detection sensor comprises a linear polarization light source, a 45-degree / 45-degree / 90-degree glass prism, a sensing chip and a spectrum analyzer and is characterized in that a silicon circulation tank is arranged on the sensing chip; the sensing chip comprises a glass sheet, a gold layer coated on the surface of one side of the glass sheet as well as a microcystin molecularly imprinted membrane modified on the surface of the gold layer. The optical detection sensor disclosed by the invention has the beneficial effects that firstly, a molecular imprinting technique is adopted for forming a sensitive layer of the sensor, so that specific recognition and adsorption efficiency for the microcystin are enhanced, the specificity of detection means is improved, and the detection time is shortened; secondly, by the use of a surface plasma optical detection technology, the sensitivity is improved, and the performance of the sensor is improved; thirdly, the whole sensor is simple and quick to operate, is low in requirement on operators, and provides effective help for highly sensitive detection of the microcystin.

Description

technical field [0001] The invention relates to a microcystin optical detection sensor. Background technique [0002] The middle and lower reaches of the Yangtze River is one of the areas where freshwater lakes are concentrated in my country. In the past few decades, human activities have had a great impact on the lake ecosystem in this area. The most prominent is the increasing eutrophication of suburban lakes. Lakes, reservoirs and rivers receive excessive nutrients such as nitrogen and phosphorus, which changes the ecological structure and function of the water body, resulting in the abnormal growth of algae, especially cyanobacteria, and the phenomenon of cyanobacteria blooms. The frequent occurrence of harmful algae blooms caused by the aggravation of eutrophication in water bodies has become an environmental problem of general concern. When the cyanobacteria bloom is severe, it not only affects human senses and destroys a healthy and balanced aquatic ecosystem, but al...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/25G01N21/41
Inventor 周连群何皓姚佳郭振张威李传宇董文飞
Owner SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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