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Quick detection agent of staphylococcus intestinal toxin

A technology of staphylococcus intestine and detection reagent, which is applied in biological tests, measuring devices, material inspection products, etc., can solve the problems of inability to meet rapid detection, time-consuming and labor-intensive, etc., and achieves short detection time, convenient use, and simple operation procedures. Effect

Inactive Publication Date: 2007-05-02
吴斌 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The whole process takes about 10 days, which is time-consuming and laborious, and cannot meet the requirements of rapid detection

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Example 1 The detection reagent strip of the present invention is composed of a sample injection port to be tested, a viewing window, a detection line, and a quality control line. The support of the reagent strip is a nitrocellulose membrane, which is divided into three areas, the two ends are the sample area and the waste liquid area respectively, and the middle part is the experimental reaction area. There are two bands with reagent labels in the experimental reaction area, and the two bands contain colloidal gold-labeled anti-staphylococcal enterotoxin monoclonal antibody, colloidal gold-labeled quality control antigen and antibodies to the corresponding quality control antigen. The usage amount of the above three reagents was 100 μL, and the concentrations were all 1:100u. After the labeling of each band was completed, the nitrocellulose membrane was blocked with 1% calf serum albumin and finally sealed in an aluminum foil bag dry.

Embodiment 2

[0015] Example 2 The usage amount of the four reagents was 80 μL, and the concentrations were all 1:100u. Other contents are the same as those of Embodiment 1.

Embodiment 3

[0016] Example 3 The usage amount of the four reagents was 90 μL, and the concentrations were all 1:120u. Other contents are the same as those of Embodiment 1.

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PUM

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Abstract

A reagent bar used for quickly detecting staphylococcus enterotoxin consists of reagent, reagent bar and micro-scale pipet. It is featured as using cell clasmatosis solution as reagent and carrying 20 micron L of specific single clone antibody of anti-staphylococcus enterotoxin, collaurum labeled single clone antibody of anti-staphylococcus enterotoxin, collaurum labeled quality control antigen and antibody of relevant antigen on reagent bar.

Description

technical field [0001] The invention relates to a reagent strip for detecting staphylococcus enterotoxin in food and drinking water. Background technique [0002] Staphylococcal enterotoxin (SE) is a toxic protein produced by Staphylococcus aureus, and serotypes A, B, C, D, E, F (also known as TSST-1) and G, H, I have been reported. All types of enterotoxins can cause food poisoning and nosocomial infections. Among them, type A is the most common, followed by types B and D. Staphylococcal enterotoxin is a strong mitogen and superantigen, has a wide range of immunomodulatory effects, and its properties are stable and easy to mass-produce and make Aerosols have become an important biological warfare agent. At present, the detection of staphylococcal enterotoxins is mainly carried out in the laboratory, that is, the test results are finally obtained through the steps of pre-enrichment, selective enrichment, isolation and identification, and animal testing. The whole process ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/569G01N33/558G01N33/531G01N33/52
Inventor 吴斌李晓岚秦成
Owner 吴斌
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