Staphylococcus aureus enterotoxin B (SEB) immune preparation and its preparation method and use

A technology for staphylococcal intestinal and immune preparations, applied in the directions of botanical equipment and methods, biochemical equipment and methods, and applications, can solve problems such as allergic reactions, aseptic suppuration, granulomas, etc., and achieve great development and utilization value. , the effect of enhancing immunogenicity
CN103160519AInactive Publication Date: 2013-06-19张婉茹
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
张婉茹
Publication Date
2013-06-19
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a staphylococcus aureus enterotoxin B (SEB) immune preparation SEB2-HSP65 and its preparation method and use. The preparation method comprises the following steps of modifying a SEB gene into a SEB2 gene, fusing the SEB2 gene and a gene of a heat shock protein HSP65 to obtain a core gene segment SEB2-HSP65, and carrying out expression of the core gene segment SEB2-HSP65 to obtain a recombinant fusion protein SEB2-HSP65. The recombinant protein SEB2 does not have a TCR cell receptor binding capacity thereby solving the problem that the existing SEB immune preparation produces a large amount of inflammatory factors, and the HSP65 enhances immunogenicity of the recombinant fusion protein SEB2. The recombinant fusion protein SEB2-HSP65 as an immune preparation for animal immunization does not need any immunologic adjuvants, can produce high-titer antibodies, can help animals to resist 5*LD50SEB toxin attack and has a protection rate of 100%. The recombinant fusion protein SEB2-HSP65 has large development and use values.
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Description

technical field

[0001] The invention relates to an immune preparation of Staphylococcus aureus enterotoxin B, a preparation method and application of the medicine. Background technique

[0002] Staphylococcus can be divided into Staphylococcus aureus, Staphylococcus epidermidis and Staphylococcus saprophyticus. Food poisoning is mainly caused by enterotoxin produced by Staphylococcus aureus. Staphylococcus aureus is widely present in air, soil, water and objects. The detection rate is also quite high on the body surface of humans and domestic animals and the cavity that communicates with the outside world. The antigenic structure of Staphylococcus is relatively complex. After the cell wall is hydrolyzed, two antigenic components can be obtained by precipitation method, namely protein antigen and polysaccharide antigen. The protein antigen is mainly Staphylococcus protein A (SPA for short), which is a surface antigen. All strains isolated from humans have SPA, and those fr...

Claims

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