Light chain and heavy chain variable regions of anti-SEB (Staphylococcal Enterotoxin B) monoclonal antibody FMU-SEB-No.1 with high neutralizing activity

A technology of monoclonal antibody, fmu-seb-no.1, which is applied in the field of biomedicine to achieve strong neutralizing ability and high affinity

Active Publication Date: 2011-01-26
FOURTH MILITARY MEDICAL UNIVERSITY
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AI Technical Summary

Problems solved by technology

The U.S. military’s 10-year toxin warfare agent prevention and control research plan has always regarded SEB as one of the important research objects, focusing on SEB damage therapeutic antibodies and small molecule inhibitors, but there are only reports on the preparation of polyclonal antibodies (PcAbs). Sexual monoclonal antibodies (mAbs) are still in their infancy

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  • Light chain and heavy chain variable regions of anti-SEB (Staphylococcal Enterotoxin B) monoclonal antibody FMU-SEB-No.1 with high neutralizing activity
  • Light chain and heavy chain variable regions of anti-SEB (Staphylococcal Enterotoxin B) monoclonal antibody FMU-SEB-No.1 with high neutralizing activity
  • Light chain and heavy chain variable regions of anti-SEB (Staphylococcal Enterotoxin B) monoclonal antibody FMU-SEB-No.1 with high neutralizing activity

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Embodiment Construction

[0030] The present invention immunizes Balb / c mice with natural SEB, prepares a group of mouse anti-SEB monoclonal antibodies, screens out the FMU-SEB-No.1 mAb hybridoma cell line that can stably secrete high-affinity anti-SEB, and prepares Obtain high-affinity anti-SEB mAb from ascites; and confirm the uniqueness of the gene sequence and corresponding protein sequence and its CDR sequence; provide support for anti-SEB chimeric or humanized genetic engineering antibodies. The present invention will be described in detail below in conjunction with the preparation method of specific monoclonal antibodies, antibody activity detection, and sequence detection and uniqueness determination, which is an explanation rather than a limitation of the present invention. The present invention is specifically implemented according to the following steps:

[0031] 1. Preparation of mouse anti-SEB high affinity antibody FMU-SEB-No.1 mAb

[0032] 1.1 Preparation and purification of monoclonal ...

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Abstract

The invention discloses light chain and heavy chain variable regions of an anti-SEB (Staphylococcal Enterotoxin B) monoclonal antibody FMU-SEB-No.1 with a high neutralizing activity. The amino acid sequence of an FMU-SEB-No.1 mAb light chain variable region is shown in SEQ.ID.NO.1, and the amino acid sequence of an FMU-SEB-No.1 mAb heavy chain variable region is shown in SEQ.ID.NO.2. A method for the invention comprises the following steps of: preparing a group of mouse anti-SEB mAbs by using a natural SEB immune Balb / c mouse, screening hybridoma cell lines which can stably secrete high-affinity anti-SEB mAb; and preparing ascitic fluid to obtain a high-affinity anti-SEB mAb FMU-SEB-No.1. The invention is beneficial to confirming the uniqueness of a gene sequence and a corresponding protein sequence as well as a CDR sequence thereof, and providing support for researching and developing an anti-SEB mosaic or humanized genetic engineering antibody.

Description

technical field [0001] The invention belongs to the field of biomedical technology, and relates to a monoclonal antibody, in particular to the light chain and heavy chain variable regions of the high neutralizing active anti-SEB monoclonal antibody FMU-SEB-No.1, including its amino acid sequence and nucleotides sequence. Background technique [0002] Biological threat and its defense is one of the most important national security concerns in the world, and it is also one of the important fields of military medical research. Biological threats mainly include biological warfare and bioterrorism, which refers to the use of biological agents (including warfare agents) to deliberately launch attacks on specific target groups and other organisms (such as animals, plants) and facilities closely related to human production and life. . Therefore, biological threats can not only kill and injure a large number of people and animals, but also cause huge psychological panic, social cha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/12C12N15/11G01N33/577G01N33/569
Inventor 杨琨金伯泉李永明孙元杰宋朝君董邦权
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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