197 results about "Chemiluminescence assay" patented technology

The present invention provides a novel chemiluminescent reagent producing chemiluminescence in the presence of hydrogen peroxide, extent of which depends on peroxidase concentration, chemiluminescent analysis method using the same, in particular useful for detection and quantitative analysis of various types of materials by measuring peroxidase enzyme activity or enzyme immunoassay with peroxidase enzyme as the marker.More particularly, the present invention provides a chemiluminescent reagent containing, as the major ingredients, a charge-transferring complex of N,N'-disubstituted-9,9'-bisacridinium salt and N,N-disubstituted carboxylic amide compound; chemiluminescent reagent containing further containing a specific aminoalcohol compound, in addition to the above; and method for measuring peroxidase activity at a high sensitivity in the presence of a peroxide, using the above chemiluminescent reagent.Moreover, the novel chemiluminescent reagent of the present invention can enhance sensitivity of the enzyme immunoassay with peroxidase enzyme as the marker by its chemiluminescent reaction.

The invention relates to a gold nanoflower preparing method and the application of gold nanoflowers. The preparing method comprises the following steps that 1) under room temperature, polyphenol is added to water solution with the pH value of 7-12 to obtain mixed solution; 2) chloroauric acid and hydrogen peroxide are then added in sequence, shaking up is carried out to obtain reaction solution; 3) the reaction solution is centrifuged, supernatant is abandoned, and the gold nanoflowers are obtained after washing. The particle diameters of the gold nanoflowers obtained under different pH values are 50-200nm, stability is good, the gold nanoflowers can be used in a biological body as a medicine carrier and have good biocompatibility, the gold nanoflowers can be used in chemiluminescence analysis, the signal intensity of a luminal chemiluminescence system can be obviously enhanced, the gold nanoflowers can be used in surface-enhanced Raman scattering (SERS) analysis, and the sensitivity is better than that of spherical gold nanoparticles. The gold nanoflower preparing method has the advantages that raw materials are easy to get, reaction conditions are mild, efficiency is high, speed is high, and batch preparing and producing are benefited.

The invention discloses a cigarette tar extract with chemiluminescent properties and a chemiluminescent system thereof. A method of preparing the cigarette tar extract comprises the following steps: the cigarette tar is added to a reagent for extraction, and the cigarette tar extract is obtained. The cigarette tar extract is prepared in an open system under a natural light condition through ultrasonic extraction, the contained luminescent material has stable properties and is insensitive to light, oxygen, temperature and other factors. The extract has a wide luminescent response range for thepH value of a medium, and chemiluminescent reactions can occur under acidic, neutral or alkaline conditions. The raw material for extracting cigarette tar has a wide source, and the preparation methodis simple. The cigarette tar extract has a broad application prospect in the technical field of chemiluminescent analysis and detection.

The invention discloses a process for preparing a trace amount mycotoxin molecular imprinted column, which comprises the following steps of: selecting a functional monomer; evenly mixing a mycotoxin template molecule, the functional monomer, a cross-linking agent, a pore-forming agent, an initiator and an organic solvent by a certain molar ratio to prepare a molecular imprinting polymer solution; preparing a nano-solution; and dressing the nano-material and a molecular imprinting polymer on the internal surface of a glass pipe column. A method for detecting the trace amount mycotoxins includes: pumping a chemiluminescence system solution and a sample solution into a chemiluminescence analyzer respectively and detecting the mycotoxins in the sample. The molecular imprinted column of the invention has advantages of high sensitivity and accuracy. The nano-enhanced trace amount mycotoxin molecular imprinted column obtained in the invention is connected to the chemiluminescence analyzer for detecting mycotoxins, and purposes of high specificity, high sensitivity and fast detection to mycotoxins in the sample can be realized.

The invention discloses a luminol direct bonded nano gold nucleic acid analyzing probe and a novel chemiluminescence analysis method. The invention provides the luminol direct bonded nano gold nucleic acid analyzing probe, a chemiluminescence analysis method based on the luminol direct bonded nano gold nucleic acid analyzing probe and a kit for constructing the analyzing method; the analyzing probe comprises nucleic acid which is directly bonded by luminol and marked by nano gold; and luminol direct bonded nano gold is obtained by reducing chloroauric acid at one step by luminol. The chemiluminescence analysis method based on the nucleic acid analyzing probe of the invention has the advantages of high sensitivity (for example, specific sequence single-chain DNA detection limit can reach 1.9*10<-16>mol/L), wide linear range, high repeatability, simple operation, low cost, and the like, can be used for the detection of DNA, RNA and adapter corresponding ligands in various samples and has wide application prospect in the fields of clinical diagnosis and treatment, pharmaceutical analysis, food safety detection, environmental monitoring, and the like.

The invention discloses a method for qualitatively and quantitatively detecting a target substance to be detected in blood serum by utilizing light initiated chemiluminescence immune assay, and the method comprises the following steps of placing luminous microspheres coating a substance antibody, biotin-coated target substance antibody and optical sensitization microspheres wrapped by streptavidin (SA) into a specimen to be detected to perform a primary immunity reaction and detection, and the method also comprises the step of placing anti-He agent into the reaction system to facilitate the target substance immune superimposition reaction, and carrying out the secondary optical excitation chemicalluminescence immune detection. A purpose for comprehensively correcting a hooks effect can be realized by comprehensively analyzing detection results of twice light initiated chemiluminescence assay (LiCA), and the analysis process comprises the following steps of classifying the specimen to be detected into five concentration intervals such as cathode, low anode, middle anode, high anode and ultrahigh anode according to the signal characteristics of the primary LiCA detection and the secondary LiCA detection; and quantitatively analyzing the specimen in the low anode concentration interval and the ultrahigh anode concentration interval according to the primary LiCA detection. The method has the characteristics of accuracy in result, simplicity and convenience in operation, wide application range and the like.

The invention discloses a chemiluminescence method for detecting an organophosphorus pesticide, and belongs to the field of pesticide residue analysis and the technical field of chemiluminescence analysis. The method comprises the following steps: 1, selecting a chemiluminescence system; 2, selecting a sensitizer; 3, performing single factor and orthogonal tests; 4, performing interference tests; 5, drawing a standard curve; 6, analyzing a sample; 7, researching a chemiluminescence mechanism; and 8, researching a relationship between a structure of the pesticide and chemiluminescence. The method has the advantages of simple and quick operation, wide linear range and low cost; an inorganic salt NaCl serves as the sensitizer of the system for the first time so as to improve the sensitivity of the system; and the method is suitable for detecting residue of organophosphorus pesticides with similar structures.

The invention provides a light-activating chemiluminescence immunity analysis kit for serum specific IgE (immunoglobulin E). The kit adopts a luminous microsphere coated with a specific single-component allergen (prepared by naturally purification or recombination), a biotin-labeled anti-human-IgE antibody and a light-sensitive microsphere precoated with streptavidin to form detecting reagents. Firstly, to-be-detected serum, the luminous microsphere coated the allergen and the a biotin-labeled anti-human-IgE antibody are incubated together, and the specific IgE antibody in the to-be-detected serum is bound with an antigen molecule on the surface of the luminous microsphere and the biotin-labeled anti-human-IgE antibody respectively; secondly, the light-sensitive microsphere precoated with streptavidin is added for continuous incubation, and is bridged with the luminous microsphere through the binding of biotin and streptavidin; thirdly, a light-activating chemiluminescence analyzing instrument is used for detecting the strength of light signals. The kit has various advantages of being easy and quick to operate, facilitating automation and quantitative analysis, and the like.

The invention relates to a luteinizing hormone nano-magnetic particle chemiluminescence assay kit and a preparation method thereof and an assay method thereof. The luteinizing hormone nano-magnetic particle chemiluminescence assay kit comprises a solution containing a fluorescein-labeled luteinizing hormone antibody, a suspension coated with magnetic particles of an anti-fluorescein antibody, and a solution containing an alkaline phosphatase-labeled luteinizing hormone antibody, wherein the alkaline phosphatase-labeled luteinizing hormone antibody is formed by connecting an alkaline phosphatase with a luteinizing hormone antibody through SMCC (4-(N-maleimidomethyl) cyclohexyl-1-succinimide carboxylate) and 2IT (2-imido sulfane hydrochloride). The luteinizing hormone nano-magnetic particle chemiluminescence assay kit can be used for quantitative determination of the luteinizing hormone at lower cost and higher accuracy and precision.

The invention discloses a magnetic particle chemiluminescence assay kit for detecting angiotensin II. The kit comprises magnetic particle suspension coupled with anti-rabbit angiotensin II antibody, a calibrator, angiotensin II antigen coupled with biotin, horse radish peroxidase labeled streptavidin, enhanced chemiluminescent A, enhanced chemiluminescent B and lotion. The invention has the advantages that: the kit can be operated conveniently and automatically, a detection result is high in repeatability and accuracy, detection time is short, the stability can be kept for twelve months, and radio contamination can be avoided. During testing, a sample is introduced by a one-step method, namely the magnetic particle suspension, a sample to be tested, the angiotensin II antigen coupled with biotin, and the horse radish peroxidase labeled streptavidin which take part in reaction are added into a reaction cup in one operation step, so operation steps and reaction time are reduced, and errors brought in an operation process are reduced.

The invention relates to a free triiodothyronine nanometer magnetic particle chemiluminescence assay kit and a preparation method thereof and a detection method thereof. The free triiodothyronine nanometer magnetic particle chemiluminescence assay kit comprises first reagents, second reagents and magnetic separation reagents, wherein the first reagents comprise fluorescein-labeled free triiodothyronine resistance antibodies, buffer solutions with potential of hydrogen(pH) 7-9, and the concentration of the fluorescein-labeled free triiodothyronine resistance antibodies is 0.5mug/mL-1mug/mL; the second reagents comprise an alkaline-phosphatase-labeled free triiodothyronine antigens, buffer solutions with pH 7-9, and the concentration of the alkaline-phosphatase-labeled free triiodothyronine antigens is 0.02 mug/mL-0.1mug/mL; the magnetic separation reagents are suspension liquid of magnetic particles covered with fluorescein-resistance antibodies. The free triiodothyronine nanometer magnetic particle chemiluminescence assay kit and the preparation method thereof and the detection method thereof have the advantage of enabling the free triiodothyronine to be quantificationally detected on the condition of lower cost, higher accuracy and higher precision.

The invention belongs to the field of analytical chemistry and particularly relates to a method for detecting glycosylated hemoglobin by chemiluminescence. The method adopts the principle that chloroauric acid is reduced by luminol to obtain luminol colloid gold nanoparticles LumAuNPs, and LumAuNPs are modified with aptamer complementary sequences DNA to obtain chemiluminescent probes; aptamers DNA are fixed by taking magnetic beads as carriers to obtain DNA modified magnetic beads; the chemiluminescent probes and the DNA modified magnetic beads are incubated to obtain chemiluminescence sensors; when a target object (glycosylated hemoglobin) exists, the aptamers DNA and glycosylated hemoglobin act with each other, and the chemiluminescent probes drop off the surfaces of the magnetic beads; and after magnetic separation, hydroxylamine-O-sulfonic acid is added into a separating medium, and LumAuNPs-hydroxylamine-O-sulfonic acid is taken as a chemiluminescent system, chemiluminescence assay is performed, and the target glycosylated hemoglobin is detected according to the generated chemiluminiscence. The method has the advantages of being simple and being high in sensitivity.

The invention discloses a set of sulfamethazine specifically-bound aptamers and a screening method and applications thereof. The aptamer for identifying sulfamethazine, disclosed by the invention, is a single-stranded DNA molecule shown in any one of formulas (1)-(4): (1) a single-stranded DNA molecule shown in a sequence 3 in a sequence table; (2) a single-stranded DNA molecule shown in a sequence 1 in the sequence table; (3) a single-stranded DNA molecule shown in a sequence 2 in the sequence table; and (4) a single-stranded DNA molecule shown in a sequence 4 in the sequence table. Experiments show that a directly competitive chemiluminescence analysis method established based on biotinylated aptamers and used for detecting sulfamethazine provides a basis for the practical application of the aptamers of sulfamethazine, and provides technical support for more efficient and extensive monitoring of antimicrobial drug residues.

The invention provides an analysis method for using magnesium-aluminium carbonate hydrotalcite to catalyze luminol-hydrogen peroxide chemiluminescence. The luminol-hydrogen peroxide chemiluminescence analysis method is automatically carried out by a BPCL ultra-weak chemiluminescence analyzer, wherein solution to be tested, testing luminous solution and ultrapure water are respectively loaded in three storage bottles, a luminol-hydrogen peroxide chemiluminescence reaction system comprises a sensitizing catalyst solution injection port, a flow cell, a photomultiplier and a BPCL tester, and the analysis method is characterized in that the magnesium-aluminium carbonate hydrotalcite is adopted as sensitizing catalyst. Compared with carbonate catalyst in catalyzing the luminol-hydrogen peroxide chemiluminescence system, the magnesium-aluminium carbonate hydrotalcite remarkably enhances the effect of catalyzing luminol-hydrogen peroxide chemiluminescence, and moreover, the magnesium-aluminium carbonate hydrotalcite has the advantages of low cost, environment-friendliness and the like, is easy to synthesize, and has a broad application prospect in environment, bioanalysis and the like.

The invention discloses a photo-thermal release signal enhanced type thyroglobulin electrochemiluminescence immunosensor. According to a construction method of the sensor, a silicon-based titanium dioxide magnetic bead is used as a substrate to fix a thyroglobulin antibody, mesoporous silica marked thyroglobulin adsorbing bipyridyl ruthenium is used as a signal probe, and a competitive immunosensor is constructed through specific recognition between an antigen and the antibody. An electrode for modifying the antibody is placed into a mixed solution containing different concentrations of free antigens and a certain amount of signal probes, so that the free target antigen and the signal probe compete to bind the antibody, and a certain luminous intensity can be generated by combining the electrode of the signal probe. Under the irradiation of an 808 nm infrared laser, a sensing interface is rapidly heated, the dipyridyl ruthenium adsorbed by the mesoporous silica is released, and a luminous signal is remarkably enhanced along with the temperature rise, so that signal amplification is realized. As the concentration of the free target is increased, the number of the signal probes combined with the antibody is reduced, the luminous intensity is also reduced, and an electrochemiluminescence analysis method for the thyroglobulin can be established on the basis of the phenomenon.

The invention discloses a cardiac troponin I magnetic particle chemiluminescence assay kit and a usage method thereof. The kit includes a plurality of reagent strips composed of a plurality of side-by-side hole sites. The reagent strip includes a cTnI magnetic microparticle reagent and a cTnI labeled reagent and further includes a sample diluent, a calibration product, a magnetic bead cleaning solution and a pre-exciting solution. A magnetic rod sleeve is arranged in one hole site on the reagent strip. By combination of a chemiluminescence technology and immunomagnetic particles, the cardiac troponin I magnetic particle chemiluminescence assay kit has higher detection sensitivity and specificity and better performance parameters; the flexibility is high; different tests can be performed simultaneously, a single test can also be carried out, so that shorter test time can be obtained, and the product cost is greatly reduced.

The invention relates to a magnetic particle chemiluminescent analyzer cleaning device and method, and belongs to the technical field of magnetic particle cleaning. The magnetic particle chemiluminescent analyzer cleaning device comprises a first power device, a cleaning needle fixing plate driven by the first power device to move up and down, and cleaning needles arranged on the cleaning needle fixing plate. A reaction cup support plate is arranged below the cleaning needles and provided with a containing groove for containing a reaction cup. A second power device is arranged on the reactioncup support plate, and a power shaft of the second power device is connected with a magnet fixing plate. The magnet fixing plate is located on one side of the containing groove and is driven by the second power device to move up and down. The position, corresponding to the reaction cup, on the magnet fixing plate is provided with a magnet. The magnetic particle chemiluminescent analyzer cleaning device and method have the beneficial effects that the problem that magnetic particles are hard to scatter after being gathered is solved, clean and thorough cleaning of the magnetic particles is guaranteed, and accordingly accuracy and stability of the analysis result are guaranteed.

The invention relates to an automatic bovine cell factor chemiluminescence immunoassay detection method based on magnetic particles. Due to a sandwich immunoassay method, a magnetic ball serves as a fixed carrier of an antibody, and flow injection is combined to be applied to chemiluminescence analysis, so that the high-sensitivity automatic bovine cell factor chemiluminescence immunoassay detection method is built. An immunosensor is manufactured by activating the magnetic ball with the carboxyl, then covalently connecting the antibody to the surface of the magnetic ball and closing residual active sites; when being applied to detection of bovine cell factors, the immunosensor is fed into an immune micro reactor after being mixed with an antigen and the antibody which is labeled with horseradish peroxidase; the immunosensor is incubated on line to form a sandwich compound, and the sandwich compound is fed into a chemiluminiscence substrate; a luminous signal is immediately acquired, so that quick detection can be realized. The automatic bovine cell factor chemiluminescence immunoassay detection method has the characteristics of short analysis time, simplicity in operation, high specificity, high sensitivity and the like and can be used for quantitative detection of the bovine cell factors and researches on immunomechanisms of the bovine cell factors.

The invention discloses a full-automatic chemiluminescent analyzer. The full-automatic chemiluminescent analyzer comprises a reagent cabin, a sample cabin, a reaction cup cabin, a cup delivery mechanism, a reagent sample adding arm, an incubation disk, an evenly mixing mechanism, a colorimetric determination system and a detection analysis system. According to the full-automatic chemiluminescent analyzer disclosed by the invention, a colorimetric determination unit and a chemiluminiscence detection unit are combined, a colorimetric method and a chemiluminescence analysis experiment can be simultaneously performed on one platform, multi-channel divided flow detection can be implemented, a key technology for one sample to detect a plurality of indexes is broken through, a detection method can be more optimally selected, and accuracy and reliability of detection results are ensured.

The invention relates to a horizontal reciprocating swinging device, which is mainly applied to uniform mixing, or cell culture, and the like, of a plurality of liquid compounds for bioluminescence analysis, biochemiluminescence analysis, chemiluminescence analysis, enzyme linked immunosorbent assay, radioimmunoassay, and the like. The swinging device comprises a driving motor, an eccentric wheel structure, a revolving crank structure, a linear swinging sample plate, and a base support structure equipped with a guiding shaft, wherein the housing of the driving motor is in screw connection with the base support; the linear swinging sample plate is in screw connection with a linear bearing and horizontally swings along a linear guide in a single direction. According to the swinging device, rotational motion of a motor shaft is transferred to another rotation shaft fixed to the eccentric wheel structure via a synchronous belt, and the rotation shaft is connected to the linear swinging sample plate via the revolving crank structure to convert the rotational motion of the motor into linear horizontal reciprocating swinging motion. The swinging device is flexible in movement and convenient to use; a hinged shaft of a swing arm is supported by a pair of bearings; and the swinging device has small friction, high rotating accuracy, and long service life. The swinging device is simple and compact in structure, small in size, and high in working reliability and stability; the cost is relatively low; and the use cost for device preparation and a user is reduced.