Trace amount mycotoxins molecular blotting column preparation method and application

A molecularly imprinted column and mycotoxin technology, which is applied in the preparation of trace mycotoxin molecularly imprinted column, and in the field of trace mycotoxins, can solve the problems of common cross-reaction, achieve high sensitivity and detection range, fast and simple operation, and good repeatability sexual effect

Inactive Publication Date: 2008-11-19
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the main difficulties that limit the detection and research of mycotoxins are: there are many structural similarities, and cross-reactivity is common; toxin detection is more easily affected by other interfering substances that are difficult to remove in the extract, etc.

Method used

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  • Trace amount mycotoxins molecular blotting column preparation method and application
  • Trace amount mycotoxins molecular blotting column preparation method and application
  • Trace amount mycotoxins molecular blotting column preparation method and application

Examples

Experimental program
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Effect test

Embodiment 1

[0036] A method for preparing a molecular imprinted column for detection of trace amounts of ochratoxin A, comprising the following steps:

[0037] (1) Select the functional monomer methacrylic acid (MAA) that can synthesize molecularly imprinted polymers with ochratoxin A;

[0038](2) Preparation of carbon nanotube solution: Add 2 mg of multi-walled carbon nanotubes (MWCNTs) to 1 ml of dimethyl sulfoxide solution under ultrasonic stirring to obtain a black suspension, MWCNTs solution;

[0039] (3) Take 20 μl of the prepared MWCNTs solution and sonicate for 30 minutes to obtain a uniformly dispersed MWCNTs solution;

[0040] (4) template molecule ochratoxin A, functional monomer methacrylic acid (MAA), crosslinking agent ethylene glycol dimethacrylate (EGDMA), porogen chloroform, initiator azobisisobutyronitrile, The organic solvent dichloromethane is mixed uniformly in a molar ratio of 0.1:1:0.5:35:0.05:2.0 to obtain an ochratoxin A molecularly imprinted polymer solution; ...

Embodiment 2

[0050] A method for preparing a deoxynivalenol molecularly imprinted column, comprising the following steps:

[0051] (1) Select functional monomer acrylic acid that can synthesize molecularly imprinted polymers with deoxynivalenol;

[0052] (2) Preparation of nano-gold solution: take 50mL of 0.01% HAuCl4 and heat to boiling, quickly add 1mL of 1% sodium citrate solution for reduction, the prepared bright rose red is nano-gold solution, put it in a 4°C refrigerator for future use;

[0053] (3) Take 20 μl of the prepared nano-gold solution, and sonicate for 20 minutes to obtain a uniformly dispersed nano-gold solution;

[0054] (4) Template molecule deoxynivalenol, functional monomer acrylic acid, crosslinking agent trimethylolpropane trimethacrylate, porogen dichloromethane, initiator azobisisobutyronitrile, organic solvent Dichloromethane is mixed uniformly at a molar ratio of 0.5:1:3:45:0.1:2.2 to obtain a deoxynivalenol molecularly imprinted polymer solution;

[0055] (5)...

Embodiment 3

[0064] A method for preparing a fumonisin molecularly imprinted column, comprising the following steps:

[0065] (1) Select the functional monomer 4-vinylpyridine that can synthesize molecularly imprinted polymers with fumonisins;

[0066] (2) Preparation of nano-platinum solution: get 4ml of 5% H 2 PtCl 6 ·6H 2 The O solution was added to 340ml of double-distilled water, and then heated while stirring at 80°C. After adding 60ml of 1% sodium citrate solution, the resulting solution, namely the nano-platinum solution, was kept at 80±0.5°C for about 4 hours. The process is recorded by adsorption spectroscopy, when PtCl 6 2- When the adsorption band disappears, it indicates that the reaction is over, that is, the nano-platinum solution is obtained;

[0067] (3) Take 20 μl of the prepared nano-platinum solution and ultrasonicate for 40 minutes to obtain a uniformly dispersed nano-platinum solution;

[0068] (4) Template molecule fumonisin, functional monomer 4-vinylpyridine,...

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Abstract

The invention discloses a process for preparing a trace amount mycotoxin molecular imprinted column, which comprises the following steps of: selecting a functional monomer; evenly mixing a mycotoxin template molecule, the functional monomer, a cross-linking agent, a pore-forming agent, an initiator and an organic solvent by a certain molar ratio to prepare a molecular imprinting polymer solution; preparing a nano-solution; and dressing the nano-material and a molecular imprinting polymer on the internal surface of a glass pipe column. A method for detecting the trace amount mycotoxins includes: pumping a chemiluminescence system solution and a sample solution into a chemiluminescence analyzer respectively and detecting the mycotoxins in the sample. The molecular imprinted column of the invention has advantages of high sensitivity and accuracy. The nano-enhanced trace amount mycotoxin molecular imprinted column obtained in the invention is connected to the chemiluminescence analyzer for detecting mycotoxins, and purposes of high specificity, high sensitivity and fast detection to mycotoxins in the sample can be realized.

Description

technical field [0001] The invention relates to the technical field of mycotoxin detection, more specifically to a method for preparing a trace mycotoxin molecular imprinting column, and also relates to a method for detecting trace mycotoxins in a sample by using the molecular imprinting column. Background technique [0002] Mycotoxin is a secondary metabolite produced by some fungi (mainly Aspergillus, Penicillium and Fusarium) during the growth process, which can easily cause pathological changes and physiological metamorphosis in humans and animals, and is highly toxic. Based on chemical structure, an estimated 400 fungal metabolites are toxic to humans and animals. Representative mycotoxins are aflatoxins, ochratoxins, patulin, trichothecenes, zearalenone, fumonisins, versicolor, fusarium, citrinin Su and so on. Ingestion of mycotoxin-contaminated food by humans or animals, or through inhalation and skin contact with mycotoxins can cause a variety of poisoning symptoms...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/34G01N21/76G01N30/50
Inventor 黄加栋裴梅山于京华汪世华张瑾王元秀葛慎光朱元娜张丽娜席志芳矫强李强何文兴宋晓妍孙纳新叶春江刘月辉王军李红梅李殿香秦余香张桂香杨新超
Owner UNIV OF JINAN
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