12 results about "Patulin" patented technology

The invention provides an effective and safe green bactericide and application thereof. The green bactericide is a composition containing methyl furoate. Mycelium growth of penicillium expansum is remarkably inhibited through methyl furoate treatment, and toxin synthesis can be completely inhibited under the median lethal concentration. Therefore, the methyl furoate can be used for preparing a novel antifungal preservative, and is applied to the field of prevention and control of fruit and vegetable postharvest fungal diseases and toxin synthesis.

The invention relates to a preparation method of a photoelectrochemical sensor for detecting patulin. The patulin is a mycotoxin produced by a plurality of penicillium, aspergillus and ascomycetes, and is easy to dissolve in water. The wide pollution of the strain to agricultural products forms a significant health risk to consumers. Therefore, it is necessary to develop a method for rapidly detecting patulin. According to the research, a novel dual-mode aptamer sensor is constructed, and ultra-sensitive detection of patulin is realized by integrating photoelectrochemical and colorimetric sensing technologies; the sensor adopts zinc phthalocyanine sensitized sulfur indium zinc as a high-performance photoactive material, and shows enhanced light absorption capability and charge separation efficiency; the lanthanum-doped nickel cobaltate nano-enzyme material is used as a bifunctional signal probe, so that the light current of a photosensitive material can be effectively quenched, and the lanthanum-doped nickel cobaltate nano-enzyme material can be used as a nano-enzyme to catalyze colorimetric reaction; through a cyclic amplification strategy assisted by exonuclease III, the sensor can detect patulin with high sensitivity, and the absorbance of an o-phenylenediamine oxidation product is weakened along with the increase of the concentration of patulin and the increase of the light current intensity; the aptamer sensor has an ultra-wide linear range of 0.00001-100 ng / mL, the detection limits respectively reach 0.0027 pg / mL (photoelectrochemistry) and 0.03 ng / mL (colorimetric method), and meanwhile, the aptamer sensor shows excellent selectivity, stability and reproducibility; a satisfactory recovery rate is achieved in actual apple and hawthorn juice sample detection, and it is proved that the method has important application value in the field of food safety monitoring.

The invention relates to the technical field of health intervention food processing, and discloses application of selenium-enriched saussurea involucrata fermented Chinese wolfberry primary pulp in preparation of liver protection products. The product is rich in organic selenium, polyphenol, lactic acid bacteria and other beneficial active components, can effectively improve the activity of antioxidant related enzymes in the liver, reduce the generation of malondialdehyde, relieve oxidative damage of patulin to the liver, and can improve the antioxidant activity of the liver by improving the level of an anti-inflammatory factor IL-10 and reducing the levels of proinflammatory factors IL-6, IL-1beta and TNF-alpha. The inflammatory reaction is relieved. The selenium-enriched saussurea involucrata fermented wolfberry puree provides a potential novel diet strategy for patients with patulin-induced liver injury, and has a considerable injury recovery effect and a wide application prospect.

The application discloses a method for extracting and purifying endogenous metabolites after exposure of zebra fish to patulin, which comprises the following steps: weighing a sample to be detected and adding acetonitrile-water mixed extraction liquid, oscillating and centrifuging to obtain extraction liquid and residues, taking out the extraction liquid, repeatedly extracting the residues, combining the extraction liquid, adding ammonium acetate, oscillating and centrifuging to obtain upper acetonitrile phase and lower water phase, taking the water phase layer to pass through a diatomite column, balancing, using pure acetonitrile as an eluent to perform elution, obtaining eluent, adding the acetonitrile phase to neutral alumina powder, oscillating and then low-temperature standing, then low-temperature high-speed centrifuging to obtain supernatant, combining the obtained eluent and the obtained supernatant, nitrogen blowing to near dryness, using a methanol-water mixed solution to constant volume, then high-speed centrifuging, and finally being ready for analysis. The application has high detection efficiency, can determine important biomarkers generated after exposure of zebra fish to patulin, and can provide a detection basis for poisoning monitoring of the compounds.

The application belongs to the technical field of biological enzyme engineering, and discloses an aldonoketoreductase GCAKR capable of degrading patulin and an application thereof. The application first proposes the aldonoketoreductase GCAKR capable of degrading patulin. The aldonoketoreductase GCAKR provided by the application solves the problems of low efficiency, secondary pollution and limited application in the existing PAT removal technology, and has the advantages of simple and efficient preparation method, wide application range, safe and efficient removal of PAT pollution in food and environment, and provides a new technical means and research basis for food safety production and environmental remediation.

The present application belongs to the technical field of biological enzyme engineering, and discloses a short-chain dehydrogenase WaSDR derived from abnormal williamsmyces, and a preparation method and application thereof. The short-chain dehydrogenase WaSDR enzyme in the present application is derived from abnormal williamsmyces, and the WaSDR enzyme can not only efficiently degrade patulin, but also can significantly improve the abundance of key flavor substances, the level, richness and complexity of the flavor of the fruit juice by precisely regulating the volatile component spectrum on the basis of retaining the original flavor characteristics of the fruit juice when added to the fruit juice. The present application provides an efficient and safe enzyme preparation for the biological degradation of patulin in food, effectively controls the PAT pollution in food processing, and simultaneously realizes the synergistic improvement of the nutrition and flavor quality of the product, and has important industrial application value.

The present invention relates to bioconjugates and labeled derivatives of the adducts formed by the reaction of patulin with benzene-1,2-dithiols or their salts. Said conjugates are suitable for the production of antibodies capable of recognizing the aforementioned patulin-(benzene-1,2-dithiols) adducts with high affinity and specificity. Likewise, the present invention also relates to the use of bioconjugates of patulin-(benzene-1,2-dithiols) adducts and of labeled derivatives thereof as assay antigens. Furthermore, the present invention also relates to immunochemical methods for the analysis of patulin, using the bioconjugates and labeled derivatives and antibodies of the invention, which include a previous step to quantitatively convert patulin into these adducts. This invention also provides a kit for analyzing patulin comprising a benzene-1,2-dithiol or its salts, antibodies against patulin-(benzene-1,2-dithiols) adducts, and assay antigens.

The present invention relates to a composition for the prevention, improvement, or treatment of obesity or obesity-induced inflammation comprising patulin as an active ingredient. A composition containing patulin as an active ingredient according to the present invention has the effect of inhibiting lipid accumulation, and can inhibit lipid accumulation by regulating sugar absorption, glycerol excretion, or LDL absorption, reducing the expression of factors related to lipogenesis or lipid synthesis such as Pparγ, Fasn, Acly, C / ebpα, Fabp4, Cd36, Dgat2, or Gpat, increasing the expression of fatty acid oxidation regulators such as PGC1α or CPT1a, and improving the volume and membrane potential of mitochondria. In addition, it can inhibit the expression of pro-inflammatory cytokines or inflammatory regulators such as IL-6, TNF-α, iNOS, or Cox-2, thereby preventing, treating, or improving obesity or obesity-induced inflammation, and can be utilized as a material for food, feed, pharmaceuticals, quasi-pharmaceuticals, etc.

The application belongs to the technical field of industrial analysis and detection, and provides a cinnamon derivative based on natural mycotoxin, a preparation method and application thereof.The preparation method comprises the following steps: mixing anatumomycin solution and trans-4-methoxy acrylate solution, and then reacting to obtain the cinnamon derivative based on natural mycotoxin; the molar ratio of anatumomycin and trans-4-methoxy acrylate is 1:1-80.The cinnamon derivative based on natural mycotoxin provided by the application can be applied in inorganic powder grinding and dispersing agents containing a large number of pseudoplastic components as a molecular tool, as an in-situ molecular level viscosity sensing tool, the size of relative viscosity can be obtained without the help of external shearing, and the viscosity change can be effectively presented in a visual way, which is helpful to realize the rapid, efficient and controllable preparation of inorganic powder grinding and dispersing agents.

The invention relates to a method for rapidly detecting patulin in fruit and vegetable products, and belongs to the technical field of patulin detection. The patulin detection method mainly comprises the following steps: extracting a to-be-detected product by using an extraction reagent consisting of thiosalicylic acid, acetic acid, ethanol and ethyl acetate to obtain a to-be-detected sample, and detecting by using a high performance liquid chromatography. The method disclosed by the invention is simple to operate, capable of realizing accurate detection of patulin, free of interference and relatively high in accuracy and sensitivity.

The present invention involves the development of biopolymer-based aptasensor and the implementation of (bio)molecule analyses in a stable, inexpensive, easy-to-modify, precise and practical way, compared to the studies in the state of the art.

The invention relates to the technical field of food safety detection, and particularly discloses a portable patulin SERS (Surface Enhanced Raman Scattering) sensing detection system based on a nanocomposite substrate. The system comprises a composite SERS substrate module, a miniaturized optical detection unit, an intelligent analysis system, a data transmission module and a portable shell, wherein the SERS substrate is composed of a Fe3O4 (at) ZIF-8 / AuNPs sandwich structure, and the SERS substrate is modified by beta-cyclodextrin so as to enhance the selective adsorption of patulin. The system integrates a 785 nm laser, a temperature control sample cabin and a micro spectrometer, and combines a Raman peak intensity ratio analysis algorithm to realize on-site rapid quantitative detection of patulin in complex food samples such as fruit juice, the detection limit is as low as 0.05 [mu] g / L, the time for single detection is less than or equal to 5 minutes, and the system is suitable for rapid screening of trace toxins in the fields of food processing, circulation, supervision and the like.