21 results about "Patulin" patented technology

The invention belongs to the technical field of food safety sensing detection, adsorption and degradation, and particularly relates to an MIP / MOF / COF hybrid material and a preparation method and application thereof. Aiming at the defects and bottleneck problems of the existing patulin detection and removal technology, the preparation method comprises the following steps: firstly preparing an MOF / COF composite material, and then preparing an MIP / MOF / COF hybrid material by a sol-gel method, so that the photoelectrochemical sensor based on the MIP / MOF / COF hybrid material is constructed and is used for detecting patulin; meanwhile, based on the MIP / MOF / COF hybrid material, an attachment and degradation test platform is constructed. The prepared photoelectrochemical sensor can successfully detect trace patulin, and the hybrid material has good adsorption and degradation performance on patulin.

The invention relates to a patulin-containing apple juice matrix standard sample and a preparation method thereof, and belongs to the field of preparation of samples for testing. The matrix standard sample is prepared through a series of steps of bacterial suspension preparation, cleaning, inoculated culture, crushing and juicing, screen filtration, adsorption treatment, centrifugal purification, heating sterilization, freeze concentration, packaging and the like, and the matrix standard sample prepared through the method has the advantages of being good in uniformity and high in stability after being subjected to strict uniformity and stability test.

The application discloses a preparation method of a ratio fluorescent chromatography test strip and application in detection of patulin, and comprises the following steps: S1, preparation of blue carbon dots; S2, preparation of gold nanoclusters; S3, preparation of a ratio fluorescent probe. The application is based on a strategy of red fluorescence quenching and blue fluorescence opening. The solution of thiol functionalized AuNCs is rich in functional groups (-SH) and is combined with patulin. Red fluorescence is gradually quenched, and blue fluorescence is opened, so that the function of detecting patulin is realized. The change of fluorescence color is from red to blue, and the whole fluorescence signal response can be completed within 10 seconds. In addition, with the continuous increase of the concentration of patulin, the red fluorescence is gradually quenched, the blue fluorescence is gradually enhanced, and a series of changes in fluorescence intensity is presented under an ultraviolet lamp, so that the visual detection of patulin is realized. The application can realize convenient and rapid preliminary detection of food health.

The invention provides an effective and safe green bactericide and application thereof. The green bactericide is a composition containing methyl furoate. Mycelium growth of penicillium expansum is remarkably inhibited through methyl furoate treatment, and toxin synthesis can be completely inhibited under the median lethal concentration. Therefore, the methyl furoate can be used for preparing a novel antifungal preservative, and is applied to the field of prevention and control of fruit and vegetable postharvest fungal diseases and toxin synthesis.

The invention relates to a preparation method of a photoelectrochemical sensor for detecting patulin. The patulin is a mycotoxin produced by a plurality of penicillium, aspergillus and ascomycetes, and is easy to dissolve in water. The wide pollution of the strain to agricultural products forms a significant health risk to consumers. Therefore, it is necessary to develop a method for rapidly detecting patulin. According to the research, a novel dual-mode aptamer sensor is constructed, and ultra-sensitive detection of patulin is realized by integrating photoelectrochemical and colorimetric sensing technologies; the sensor adopts zinc phthalocyanine sensitized sulfur indium zinc as a high-performance photoactive material, and shows enhanced light absorption capability and charge separation efficiency; the lanthanum-doped nickel cobaltate nano-enzyme material is used as a bifunctional signal probe, so that the light current of a photosensitive material can be effectively quenched, and the lanthanum-doped nickel cobaltate nano-enzyme material can be used as a nano-enzyme to catalyze colorimetric reaction; through a cyclic amplification strategy assisted by exonuclease III, the sensor can detect patulin with high sensitivity, and the absorbance of an o-phenylenediamine oxidation product is weakened along with the increase of the concentration of patulin and the increase of the light current intensity; the aptamer sensor has an ultra-wide linear range of 0.00001-100 ng / mL, the detection limits respectively reach 0.0027 pg / mL (photoelectrochemistry) and 0.03 ng / mL (colorimetric method), and meanwhile, the aptamer sensor shows excellent selectivity, stability and reproducibility; a satisfactory recovery rate is achieved in actual apple and hawthorn juice sample detection, and it is proved that the method has important application value in the field of food safety monitoring.

The present invention discloses a nano-antibody for patulin and its application. The present invention uses an artificial patulin antigen (a conjugate of patulin and a carrier protein BSA) as a coating source and utilizes a natural nano-antibody library to screen the patulin nano-antibody. This method eliminates the need to immunize camelids with the artificial patulin antigen, thereby avoiding the toxic effects of patulin on animals. The patulin nano-antibody is prepared and a patulin immunoassay method is established using the patulin nano-antibody. The detection limit for patulin is 0.12 μg / mL, the half-inhibitory concentration is 0.41 μg / mL, and the linear range is 0.17-0.97 μg / mL. The method is simple, rapid, highly specific, and highly sensitive, and can be used for the rapid detection of patulin in food.

The invention relates to the technical field of health intervention food processing, and discloses application of selenium-enriched saussurea involucrata fermented Chinese wolfberry primary pulp in preparation of liver protection products. The product is rich in organic selenium, polyphenol, lactic acid bacteria and other beneficial active components, can effectively improve the activity of antioxidant related enzymes in the liver, reduce the generation of malondialdehyde, relieve oxidative damage of patulin to the liver, and can improve the antioxidant activity of the liver by improving the level of an anti-inflammatory factor IL-10 and reducing the levels of proinflammatory factors IL-6, IL-1beta and TNF-alpha. The inflammatory reaction is relieved. The selenium-enriched saussurea involucrata fermented wolfberry puree provides a potential novel diet strategy for patients with patulin-induced liver injury, and has a considerable injury recovery effect and a wide application prospect.

The invention belongs to the technical field of biology, and relates to an enzyme separated from plant lactobacillus and application of the enzyme, the enzyme is prepared through a prokaryotic expression system and can efficiently degrade patulin, 1 mg / L of enzyme can completely degrade 5 mg / L of PAT, the degradation rate is still higher than 80% when the concentration of PAT is 40 mg / L, a degradation product is identified as low-toxicity E / Z-ascladiol, the cytotoxicity is remarkably lower than that of PAT, and the enzyme can be used for efficiently degrading patulin. The enzyme separated from the plant lactobacillus, provided by the invention, provides effective theoretical support and candidates for degrading patulin in food and feed by lactic acid bacteria and biological enzymes, and provides a safe basis for performing biological detoxification by industrially using the biological enzymes and the lactic acid bacteria.

The application discloses a method for extracting and purifying endogenous metabolites after exposure of zebra fish to patulin, which comprises the following steps: weighing a sample to be detected and adding acetonitrile-water mixed extraction liquid, oscillating and centrifuging to obtain extraction liquid and residues, taking out the extraction liquid, repeatedly extracting the residues, combining the extraction liquid, adding ammonium acetate, oscillating and centrifuging to obtain upper acetonitrile phase and lower water phase, taking the water phase layer to pass through a diatomite column, balancing, using pure acetonitrile as an eluent to perform elution, obtaining eluent, adding the acetonitrile phase to neutral alumina powder, oscillating and then low-temperature standing, then low-temperature high-speed centrifuging to obtain supernatant, combining the obtained eluent and the obtained supernatant, nitrogen blowing to near dryness, using a methanol-water mixed solution to constant volume, then high-speed centrifuging, and finally being ready for analysis. The application has high detection efficiency, can determine important biomarkers generated after exposure of zebra fish to patulin, and can provide a detection basis for poisoning monitoring of the compounds.

The invention provides a photoelectrocatalytic degradation method for patulin in apple juice, which comprises the following steps: growing TiO2 on the surface of a fluorine-doped tin oxide conductive glass electrode treated by plasma, and annealing to obtain a TiO2 nanorod electrode; the preparation method comprises the following steps: growing a silicon dioxide film with the thickness of 10-40nm on the surface of a TiO2 nanorod electrode by a # imgabs0 # solution growth method, and after annealing, cleaning redundant # imgabs1 # solution to obtain a TiO2 nanorod electrode modified by a vertical pore silicon dioxide film; the TiO2 nanorod electrode is inserted into apple juice and serves as a working electrode, a platinum electrode serves as a counter electrode, an Ag / AgCl electrode serves as a reference electrode, and patulin of the apple juice is degraded through photoelectrocatalysis under irradiation of a light source. According to the TiO2 nanorod electrode prepared by the method, stable, accurate and efficient degradation of patulin can be realized while nutrient components rich in apple juice are not influenced.

The application belongs to the technical field of biological enzyme engineering, and discloses an aldonoketoreductase GCAKR capable of degrading patulin and an application thereof. The application first proposes the aldonoketoreductase GCAKR capable of degrading patulin. The aldonoketoreductase GCAKR provided by the application solves the problems of low efficiency, secondary pollution and limited application in the existing PAT removal technology, and has the advantages of simple and efficient preparation method, wide application range, safe and efficient removal of PAT pollution in food and environment, and provides a new technical means and research basis for food safety production and environmental remediation.

The present application belongs to the technical field of biological enzyme engineering, and discloses a short-chain dehydrogenase WaSDR derived from abnormal williamsmyces, and a preparation method and application thereof. The short-chain dehydrogenase WaSDR enzyme in the present application is derived from abnormal williamsmyces, and the WaSDR enzyme can not only efficiently degrade patulin, but also can significantly improve the abundance of key flavor substances, the level, richness and complexity of the flavor of the fruit juice by precisely regulating the volatile component spectrum on the basis of retaining the original flavor characteristics of the fruit juice when added to the fruit juice. The present application provides an efficient and safe enzyme preparation for the biological degradation of patulin in food, effectively controls the PAT pollution in food processing, and simultaneously realizes the synergistic improvement of the nutrition and flavor quality of the product, and has important industrial application value.

The invention provides a COFs (Covalent Organic Frameworks)-based SERS (Surface Enhanced Raman Scattering) aptamer sensor as well as a preparation method and application thereof. The preparation method comprises the following steps: preparing COF (at) Ag-cDNA (Complementary Deoxyribose Nucleic Acid) from a COFs probe loaded by silver nanoparticles, preparing Au (at) MBA (at) Ag-apt from a gold-silver core-shell probe embedded with 4-MBA, and mixing the COF (at) Ag-cDNA and the Au (at) MBA (at) Ag-apt to prepare the COFs-based SERS aptamer sensor. According to the SERS aptamer sensor based on the covalent organic framework material COFs, a COFs probe with silver nanoparticles growing on the surface and a gold-silver core-shell probe embedded with a Raman reporter molecule 4-MBA are accurately connected by utilizing a base complementary pairing principle of an aptamer and a complementary chain of the aptamer, so that the hot spot density is effectively increased, the signal intensity is remarkably enhanced, and the detection sensitivity is improved. By establishing a quantitative relation curve between Raman intensity of a characteristic signal peak and mycotoxin concentration, specific quantitative detection of patulin in fruits and vegetables is realized, and the method has the characteristics of simplicity and convenience in operation and quick response, has excellent sensitivity, stability and specificity, and has good accuracy and reliability in detection of fruit and vegetable samples.

The present invention relates to bioconjugates and labeled derivatives of the adducts formed by the reaction of patulin with benzene-1,2-dithiols or their salts. Said conjugates are suitable for the production of antibodies capable of recognizing the aforementioned patulin-(benzene-1,2-dithiols) adducts with high affinity and specificity. Likewise, the present invention also relates to the use of bioconjugates of patulin-(benzene-1,2-dithiols) adducts and of labeled derivatives thereof as assay antigens. Furthermore, the present invention also relates to immunochemical methods for the analysis of patulin, using the bioconjugates and labeled derivatives and antibodies of the invention, which include a previous step to quantitatively convert patulin into these adducts. This invention also provides a kit for analyzing patulin comprising a benzene-1,2-dithiol or its salts, antibodies against patulin-(benzene-1,2-dithiols) adducts, and assay antigens.

The present invention relates to a composition for the prevention, improvement, or treatment of obesity or obesity-induced inflammation comprising patulin as an active ingredient. A composition containing patulin as an active ingredient according to the present invention has the effect of inhibiting lipid accumulation, and can inhibit lipid accumulation by regulating sugar absorption, glycerol excretion, or LDL absorption, reducing the expression of factors related to lipogenesis or lipid synthesis such as Pparγ, Fasn, Acly, C / ebpα, Fabp4, Cd36, Dgat2, or Gpat, increasing the expression of fatty acid oxidation regulators such as PGC1α or CPT1a, and improving the volume and membrane potential of mitochondria. In addition, it can inhibit the expression of pro-inflammatory cytokines or inflammatory regulators such as IL-6, TNF-α, iNOS, or Cox-2, thereby preventing, treating, or improving obesity or obesity-induced inflammation, and can be utilized as a material for food, feed, pharmaceuticals, quasi-pharmaceuticals, etc.

The invention relates to a method for detecting trace patulin based on a bionic nanofluid sensor, and belongs to the field of detection. The preparation method comprises the following steps: modifying an anodic aluminum oxide (AAO) nano-channel membrane; growing a covalent organic framework (COFs) in situ on the surface of the AAO nano-channel film from bottom to top, and preparing a covalent organic framework nano-channel with the aperture smaller than 2 nm; gold ions grow in situ in a COFs pore channel through a post-synthesis method, and a sub-nano-channel metal covalent organic framework (Au-COF) nano-channel is prepared; the aptamer (Apt) is anchored on the surface of the Au-COF through a covalent coupling strategy, and the Apt / Au-COF / AAO nanofluid sensor is prepared. According to the invention, Au-COF is used as a nano channel, Apt is used as a recognition unit, the sensor integrates specific recognition, signal amplification and rapid detection, and high-selectivity and high-sensitivity analysis of trace patulin (PAT) in a complex food matrix is realized.

The application belongs to the technical field of industrial analysis and detection, and provides a cinnamon derivative based on natural mycotoxin, a preparation method and application thereof.The preparation method comprises the following steps: mixing anatumomycin solution and trans-4-methoxy acrylate solution, and then reacting to obtain the cinnamon derivative based on natural mycotoxin; the molar ratio of anatumomycin and trans-4-methoxy acrylate is 1:1-80.The cinnamon derivative based on natural mycotoxin provided by the application can be applied in inorganic powder grinding and dispersing agents containing a large number of pseudoplastic components as a molecular tool, as an in-situ molecular level viscosity sensing tool, the size of relative viscosity can be obtained without the help of external shearing, and the viscosity change can be effectively presented in a visual way, which is helpful to realize the rapid, efficient and controllable preparation of inorganic powder grinding and dispersing agents.

The present application belongs to the technical field of bioengineering, and particularly relates to a kind of aldehyde ketone reductase and its application.The aldehyde ketone reductase is a protein consisting of the amino acid sequence shown in SEQ ID NO.1;the coding gene of the aldehyde ketone reductase, nucleotide sequence is shown in SEQ ID NO.2.The aldehyde ketone reductase constructed in the present application has high catalytic performance, in the reaction system with aldehyde ketone reductase as biological catalyst, PAT as substrate, NADPH as coenzyme and MES buffer, 15ug / mL of patulin can be completely degraded in 4h;and 0.5ug / mL of patulin in fresh pear juice can be completely degraded in 4h.Simultaneously, the present application is safe and environmentally friendly, and the expressed and purified aldehyde ketone reductase can be effectively used to control patulin pollution in self-made pear juice, and has broad application prospect.

The invention relates to a method for rapidly detecting patulin in fruit and vegetable products, and belongs to the technical field of patulin detection. The patulin detection method mainly comprises the following steps: extracting a to-be-detected product by using an extraction reagent consisting of thiosalicylic acid, acetic acid, ethanol and ethyl acetate to obtain a to-be-detected sample, and detecting by using a high performance liquid chromatography. The method disclosed by the invention is simple to operate, capable of realizing accurate detection of patulin, free of interference and relatively high in accuracy and sensitivity.

The present invention involves the development of biopolymer-based aptasensor and the implementation of (bio)molecule analyses in a stable, inexpensive, easy-to-modify, precise and practical way, compared to the studies in the state of the art.

The invention relates to the technical field of food safety detection, and particularly discloses a portable patulin SERS (Surface Enhanced Raman Scattering) sensing detection system based on a nanocomposite substrate. The system comprises a composite SERS substrate module, a miniaturized optical detection unit, an intelligent analysis system, a data transmission module and a portable shell, wherein the SERS substrate is composed of a Fe3O4 (at) ZIF-8 / AuNPs sandwich structure, and the SERS substrate is modified by beta-cyclodextrin so as to enhance the selective adsorption of patulin. The system integrates a 785 nm laser, a temperature control sample cabin and a micro spectrometer, and combines a Raman peak intensity ratio analysis algorithm to realize on-site rapid quantitative detection of patulin in complex food samples such as fruit juice, the detection limit is as low as 0.05 [mu] g / L, the time for single detection is less than or equal to 5 minutes, and the system is suitable for rapid screening of trace toxins in the fields of food processing, circulation, supervision and the like.