104 results about "Patulin" patented technology

A preparation method and application of a molecular imprinted polymer on the silica surface for specifically adsorbing patulin belong to the technical field of bioassay. The method comprises the following steps: firstly pretreating the silica surface; siliconizing the silica surface to prepare APTS-silica and introducing amino groups; preparing ACPA-silica by dewatering and esterifying or an acyl chloride intermediate method and grafting an azo initiator on the silica surface; and finally taking the patulin analog 2-hydroxynicotinic acid as a template to replace molecules, taking acrylamide, methylacrylic acid, 4-vinyl pyridine and trifluoromethyl acrylic acid as the functional monomers and taking ethylene glycol dimethacrylate (EDMA) as a cross-linking agent to obtain the molecular imprinted polymer on the silica surface in acetonitrile and pyridine solvents by thermal initiation. The invention has the following advantages: as the pretreatment method for liquid chromatography of trace patulin in apple juice and related products, the polymer prepared by the invention can specifically adsorb the enriched patulin, the sample is simply and quickly treated and can be reused multiple times, and the polymer is resistant to acid and alkali treatment and is preserved under normal temperature, at the same time, the invention avoids the recovery loss caused by the traditional steps of removing impurities by alkaline washing by liquid-liquid extraction and removing organic solvents by rotary evaporation and reduces the usage amount of the organic solvents.

The invention provides ultrahigh cross-linked macro-porous adsorption resin which is obtained by taking a styrene monomer as a functional monomer, taking a multi-vinyl monomer as a cross-linking agent, suspending and polymerizing in the presence of a pore forming agent to obtain low-cross-linked macro-porous polystyrene white ball, reacting the obtained white ball with chloromethyl ether under the catalysis of lewis acid to obtain chloromethylation macro-porous polystyrene resin, and carrying out a Friedel-Crafts alkylation reaction on the obtained chloromethylation macro-porous polystyrene resin in the presence of a swelling agent by taking the lewis acid as a catalyst. Through adopting a novel cross-linking agent and pore forming agent system, the obtained resin has the advantages of high specific surface area and uniform pore diameter; the specific surface area is up to 1500-1800m<2> / g, the pore diameter distribution is uniform, a pore channel is dense and the average pore diameter is small; the pore diameter of the obtained macro-porous resin is rightly applicable to removal of patulin in juice and the removing efficiency is high; the resin can be used for pointedly removing the patulin which stably exists in the juice and the potential hazards on the human health, caused by the patulin in the juice, are solved; the ultrahigh cross-linked macro-porous adsorption resin has great social and economic benefits.

The invention relates to a method for removing patulin in orange juice by utilizing immobilized inactivated yeast cells of magnetic microspheres and application of the method. The method comprises the steps: adopting an inverse suspension crosslinking method, by means of glutaraldehyde crosslinking, polymerizing with chitosan molecules to prepare magnetic Fe3O4 / CTS microspheres, and optimizing the process parameters of immobilized inactivated Candida. u CICC1769 cells of the magnetic Fe3O4 / CTS microspheres, thereby utilizing the immobilized inactivated yeast cells of the magnetic microspheres to remove patulin in the orange juice. The method has the advantages of good adsorption effect, low cost and environmental protection, the efficient and rapid separation of the inactivated yeast cells and a target product can also be realized, and various adverse effects of living microorganisms on the product and the human body are simultaneously avoided.

The invention discloses a various mycotoxin quantifying detection protein chip and a kit thereof. The chip comprises a substrate and a point coating layer of arrayed mycotoxin antibodies which contains seven mycotoxin antibodies formed by uniformly distributing anti-aflatoxin B1, anti-ochratoxin A, anti-fumonisins, anti-vomitoxin, anti-zearalenone, anti-T-2 toxin and anti-patulin on the substrate. A detecting result with various indications is acquired by reacting once by using the protein chip of the invention. The method can be widely applied to the safety sanitation detection for products such as popular food, grain and oil food, feeding stuff, juice beverage and the like and can meet the requirement of the modern food industry to rapidly, simply and efficiently detect the safety of food.

It is an object of the present invention to provide nucleic acid molecules that enable highly sensitive detection of ligands (e.g., patulin). It is another object of the present invention to provide a screening method for nucleic acid molecules that enable highly sensitive detection of ligands (e.g., patulin), and a method for screening for nucleic acid molecules used for the optimization of nucleic acid molecules that enable highly sensitive detection of ligands (e.g., patulin). It is a further object of the present invention to provide a method for effectively removing ligands from samples containing ligands (e.g., patulin). According to the present invention, there is provided a loop-structured nucleic acid molecule for detection of ligands (e.g., patulin) having a DNA aptamer and a DNAzyme, wherein the sequence is modified between the DNA aptamer region and the DNAzyme.

The invention discloses a method for improving the efficacy of cryptococcus laurentii with control on the penicilliosis and the patulin of post-picked apples, belonging to the technical field of biological control. The method is carried out according to following steps: activating the cryptococcus laurentii, inoculating the cryptococcus laurentii to a trehalose supplemental medium (a carbon source comprises 0.4% of trehalose and 0.6% of glucose) for culturing, and centrifuging the cryptococcus laurentii to obtain thalli; preparing the thalli into a 1*108 pieces / ml bacterium suspension; and adding 30mul yeast bacterium suspensions to the wounds of fruit, naturally drying the fruit, putting the fruit into a plastic basket, sealing by plastic wraps, and storing the fruit at a room temperature. Using the trehalose to improve the efficacy of the cryptococcus laurentii with control on the penicilliosis and the patulin of the post-picked apples, the method for improving the efficacy of the cryptococcus laurentii with control on the penicilliosis and the patulin of the post-picked apples is friendly to environment, safe, economical, efficient and applicable, thereby having important social values and economic values.

The invention discloses a method for degrading patulin by using enzymes. The method comprises the following steps: preparing a crude enzyme preparation from thalluses of rhodosporidium paludigenum fell and tallman induced by patulin; putting the crude enzyme preparation into a liquid which is polluted by patulin and degrading patulin for 0.5-1 hour at a temperature of 38-42 DEG C, wherein each 100 microliters of the crude enzyme preparation is used for degrading the liquid, containing no more than 16 micrograms of patulin, which is polluted by patulin. The method for degrading patulin has the advantages of low cost, good degrading effect, good safety and no secondary pollution.

The invention discloses application of a yeast strain to elimination of patulin. The invention provides application of Pichia membranaefaciens to elimination of patulin. Experiments prove that Pichia membranaefaciens can quickly remove high concentration patulin in culture solution; patulin residues can not be detected four days after the Pichia membranaefaciens cells with concentration being 2*105cfu / ml are inoculated into the culture solution containing 100mu g / ml of patulin to be cultured; and patulin residues can not be detected six days after the Pichia membranaefaciens cells with concentration being 2*105cfu / ml are inoculated into the culture solution containing 200mu g / ml of patulin to be cultured. Application provided by the invention can provide a new solution to removal of patulin residues in such food as fruit juice.

The invention discloses lactobacillus plantarum with a function of removing patulin and belongs to the technical field of microorganisms. The lactobacillus plantarum disclosed by the invention has acid resistance and can be used for removing patulin from an aqueous solution. When 1*10<10>CFU / mL of lactobacillus plantarum is cultured together with 1000mu g / L patulin for 4 hours, the lactobacillus plantarum can remove more than 80% of the patulin, and when 1*10<10>CFU / mL of lactobacillus plantarum is cultured together with 1000mug / L patulin for 8 hours, the lactobacillus plantarum can remove more than 90% of the patulin. In addition, the lactobacillus plantarum has the functions of significantly reducing the patulin in apple juice and moldy apple pomace and alleviating the influence of the patulin on intestinal microflora of mice. The lactobacillus plantarum is used for removing patulin in foods or feeds and has very broad application prospect.

The invention discloses a method for detecting patulin and a special enzyme linked immunosorbent assay kit thereof. The enzyme linked immunosorbent assay kit comprises patulin monoclonal antibodies. The patulin monoclonal antibodies are secreted by patulin monoclonal antibody hybridoma cell strains PAT with collection number CGMCC No.3392. The enzyme linked immunosorbent assay kit qualitatively or quantitatively detects the patulin content of fruits or juice by mainly adopting an indirect competitive ELISA method. The kit and the detection method have low pretreatment requirement for a sample and simple sample pretreatment process, and can quickly detect a large batch of samples at the same time; and by adopting the high-specificity patulin monoclonal antibodies, the detection method is convenient and feasible and has the characteristics of high specificity, high sensitivity, high precision, high accuracy and the like. The kit plays a great role in the detection of the patulin.

The invention discloses a method for controlling apple patulin, belonging to a method for controlling plant postharvest diseases. The method provided by the invention comprises the following steps of: activating rhodotorula mucilaginosa, inoculating the rhodotorula mucilaginosa into an NYDB (Nutrient Yeast Dextrose Broth) culture medium to be cultured and centrifuging to obtain thalli; diluting the thalli by utilizing a 0.2% phytic acid solution to prepare a thalli suspension solution with the concentration of 1*10 to the eighth power per milliliter; adding 30 milliliters of microzyme suspension solution into the wounds of the fruit and naturally drying; and then putting the fruit into a plastic basket and sealing the plastic basket by a preservative film. In the method disclosed by the invention, the phytic acid and the rhodotorula mucilaginosa are combined to control the apple patulin; and the method is environmental-friendly, safe, economical, efficient and practical and has important social values and economic values.

The invention discloses a method for detecting patulin and chloramphenicol in hawthorn products. Testing is carried out by using a liquid chromatography-mass spectrometry method, the liquid chromatography conditions are as follows: the spectrum column Agilent Eclipse Plus C18 is 1.8mu m, 2.1mm*100mm, the column temperature is 33-38 DEG C, and the flowing speed is 0.2-0.5ml / min; gradient elution iscarried out with 9mmol / L ammonium acetate as a flow phase A and methanol as a flow phase B, and the sampling feeding amount is 1-2mu l; the mass spectrum conditions are as follows: an electrospray ionization source is adopted, detection is carried out in an MRM (Multiple Reaction Monitoring) mode, the temperature of a drying gas is 210 DEG C, the pressure of an atomizer is 20Psi, the flowing speed of the drying gas is 11-12L / minute, the temperature of a sheath flow gas is 350 DEG C, the flowing speed of the sheath flow gas is 10L / minute, and the voltage of a nozzle is 1000V. The method disclosed by the invention has the advantages of being simple, convenient and rapid, high in accuracy and good in stability, and is applicable to quality control on hawthorn products in laboratories, and on-batch detection with high detection efficiency can be achieved by using the method.

The invention provides a molecularly imprinted electrochemical sensor for detecting patulin. The sensor comprises a glassy carbon electrode, a thionine-Pt nanoparticle-nitrogen-doped graphene composite nanomaterial layer and a molecularly imprinted polymer layer. The molecularly imprinted electrochemical sensor can effectively amplify the signal, has high sensitivity and has good selectivity, a high recovery rate and good reproducibility. The molecularly imprinted electrochemical sensor is free of expensive large instruments, has a simple pretreatment process, ensures the accuracy of the results, realizes fast detection, greatly saves a cost and improves detection efficiency.

An aptamer for specifically detecting patulin and a method for detecting patulin using the same. The aptamer for specifically detecting patulin is a single-stranded DNA aptamer serving as a bioreceptor capable of effectively detecting patulin. Since such an aptamer for specifically detecting patulin is capable of specifically binding to patulin which is a mycotoxin having a simple chemical structure and a low molecular weight, it can be effectively employed as a bioreceptor in various bioassays for specifically detecting patulin. The aptamer for specifically detecting patulin can achieve more effective detection of patulin in apples or apple juice.

The invention relates to a method for completely removing patulin in cider made from defective apples through synchronous adsorption and mixed bacteria fermentation, and belongs to the technical field of food engineering. The method includes subjecting the defective apples to crushing, color protecting, juicing and filtering after cleaning, adjusting total sugar concentration of clear apple juice to 15-21Brix by cane sugar, adjusting pH to 3.1-3.8 by malic acid, inoculating the processed clear apple juice with saccharomyces cerevisiae and hansenula anomala, fermenting the inoculated clear apple juice at the temperature of 20-25 DEG C, stopping fermentation when the sugar degree of fermented liquid is lower than 4g / L, filtering the fermented liquid, and sterilizing the filtered liquid to obtain a cider stock solution, wherein the ratio of the viable count of the saccharomyces cerevisiae to the viable count of the hansenula anomala is (2-3):1. The method is capable of completely removing the patulin in the cider made from the defective apples, simple and convenient to operate, and safe and efficient to use, thereby being the most ideal method for removing the patulin in the cider made from the defective apples currently.

The invention discloses a kit for synchronously detecting six fungal toxins. The six toxins are aflatoxin, ochratoxin, patulin, trichothecene mycotoxin, fumonisins and zearalenone; and based on a multiple PCR detection method, the kit comprises a first primer pair, a second primer pair, a third primer pair, a fourth primer pair, a fifth primer pair, a sixth primer pair and an internal standard primer pair which can perform PCR reaction in the same reaction tube. The detection system directly targets a toxin synthesis essential gene and has high specificity; an internal reference gene is amplified synchronously, positive, negative and blank control are set simultaneously, and false negative and false positive are effectively avoided; and multiplication of PCR index guarantees the sensitivity of the method.

The invention relates to the field of microorganisms, and specifically discloses a new application of pseudomonas aeruginosa, namely the new application of the pseudomonas aeruginosa to patulin degradation. According to the invention, it is proved by experiments that the pseudomonas aeruginosa can degrade the patulin effectively. The pseudomonas aeruginosa has a good application prospect in developing both of new biodegradation microbial preparations and new biodegradation sterile preparations.

The invention discloses application of a yeast strain to elimination of patulin. The invention provides application of Candida guilliermondii to elimination of patulin. Experiments prove that Candida guilliermondii can quickly remove high concentration patulin in culture solution; patulin residues in three concentrations of culture solution can not be detected two days after the Candida guilliermondii cells with concentration being 2*105cfu / ml are inoculated into the culture solution containing 50mu g / ml of patulin, the culture solution containing 100mu g / ml of patulin and the culture solution containing 200mu g / ml of patulin respectively to be cultured. Application provided by the invention can provide a new solution to removal of patulin residues in such food as fruit juice.

The invention discloses a method for rapidly measuring patulin in fruit jam and vegetable paste, comprising the following steps: weighing 2.00g of fruit jam and vegetable paste in a centrifuge tube, adding 0.5-3mL of water, shaking, adding 5mL of ethyl acetate-normal hexane solution (95+5, v / v), shaking, simultaneously adding 0.5-2g of sea sands, 4-8g of anhydrous sodium sulfate and 0.5-2g of sodium bicarbonate, shaking vigorously for 2min, and centrifuging for 3min; and weighing 4mL of supernate until ethyl acetate activates a carbon black columella, receipting eluent by a test tube in which glacial acetic acid is added, eluting by 3-7mL of ethyl acetate, and drying; dissolving 240 mu L of acetonitrile, pipetting 150 mu L of dissolution liquid into a sample bottle, adding 50 mu L of derivatization reagent, heating by microwave for 1-7min, cooling and then measuring by gas chromatography-mass spectrometry. The method is rapid, simple, sensitive, accurate, specific and durable in confirmation and quantification, and is widely applied to the measurement filed of patulin in fruit jam and vegetable paste.

The invention discloses application of encapsulated cryptococcus laurentii to removal of patulin, and provides a method for removing patulin in a system by use of cryptococcus laurentii. The method comprises the following steps: 1) mixing cryptococcus laurentii or a suspension thereof, a sodium alginate water solution and a CaCl2 water solution to obtain cryptococcus laurentii colloidal particles; 2) uniformly mixing the cryptococcus laurentii colloidal particles with a system containing patulin and reacting so as to remove the patulin in the system. The application shows that the encapsulated cryptococcus laurentii can be used for removing the patulin in the system, and the encapsulated cryptococcus laurentii can be recovered without affecting the system.

The invention discloses a method for fast measuring patulin in apple juice. The method comprises the following steps: weighing 5.00 grams of apple and shaking the apple in a centrifuge tube; adding 5mL of ethyl acetate-normal hexane solution (95+5, V / V); shaking the centrifuge tube and adding 0.3-0.7 gram of sodium bicarbonate; fiercely shaking the centrifuge tube, carrying out centrifugation, moving 3.2mL of supernate to ethyl acetate activated Crab / C18 mixed type solid phase extraction column, using a test tube with 30mu L of glacial acetic acid to receive eluent, eluting 4-8mL ethyl acetate, injecting nitrogen for drying, adding 240mu L acetonitrile for dissolving, accurately moving 150mu L of solvent to a sample bottle, adding 50mu L of derivatization reagent; using a shock insulator for sealing, carrying out microwave heating for 1-5min, cooling gas phase color spectrum-mass spectrum measurement. The method is a fast, easy and convenient, sensitive, specific durable and quantitative method for confirmation, thus being widely applied to the field where patulin in apple juice is measured.

The invention discloses a solid phase extraction and purification column, which is used in pretreatment during detection of patulin, is mainly applied to the detection of patulin residues and belongs to the field of solid phase extraction columns. An appropriate amount of crosslinked polyvinylpyrrolidone (pvpp) is uniformly mixed with Florisil, and the mixture is filled into a solid phase extraction column hollow pipe with the volume of 15ml. Product extracting solution of hawthorn and apples directly passes through the solid phase extraction column, filtrate is collected and dried by nitrogen, volume is fixed and filtering is performed for analyzing. The column has good impurity removing effect and high yield and can effectively replace the effect of a 228AflaPat multifunctional column on the detection of the patulin. The effect of the solid phase extraction column is basically equivalent to that of the 228AflaPat multifunctional column, and the cost of the solid phase extraction column is one tenth of that of the 228AflaPat multifunctional column. The column is easy and convenient to prepare, is easy to operate, has high repeatability and is easy for mass production.

The disclosure relates to an appetizing and summer heat relieving tangxin raw cider and a high-efficiency deep processing technology thereof. The high-efficiency deep processing technology comprises the following steps: (1) preparing raw pulp; (2) detoxifying patulin; (3) inoculating and precisely fermenting; (4) performing enzymolysis of residues; (5) clarifying and filtering; (6) ageing; and (7) canning after adsorbing and clarifying. According to the disclosure, aiming at a phenomenon that cider is muddy after being stored, it is solved by adsorption with a multi-stimuli responsive nano fiber modified resin. Exterior stimuli include change in environment temperature or acidity-basicity (pH value), light, electric field, magnetic field or specific molecules. In response to exterior stimuli, nano fiber may include physicochemical properties such as size, hydrophilicity and hydrophobicity, refractive index, transmittance, mechanical strength, color or conductivity.

The invention belongs to the field of analytical chemistry, and particularly relates to a method for easily and conveniently detecting patulin. The method comprises the steps that firstly, a patulin aptamer is fixed in a 96-pore plate micropore, an invertase modified aptamer complementary sequence serves as a probe, and the probe is fixed in the 96-pore plate micropore through DNA hybridization. When the target object patulin exists, recognition action is conducted between the patulin and the patulin aptamer, disengagement of the invertase modified aptamer complementary sequence probe is achieved, a disengaged probe solution is taken to be put into a small centrifugal tube, saccharose is added and converted into glucose under the action of invertase on the probe, a glucometer is utilized to serve as a detecting instrument, and determination of the patulin is achieved.

The invention belongs to the technical field of food safety, and particularly relates to a method of removing patulin in apple juice. The method comprises the following steps: (1) mixing edible corn flour with distilled water according to the mass ratio of 1: 50-60, stirring for 8-10 minutes, standing for 10-15 minutes, carrying out pumping filtration separation, repeatedly operating for 3-4 times, and sieving after drying at the temperature of 60 DEG C to obtain water insoluble corn flour with different particle sizes; and (2) adding the water insoluble corn flour to the apple juice polluted by patulin, stirring, carrying out adsorption treatment for 5-12 hours at the temperature of 20-40 DEG C, and carrying out filtering separation to realize removal of the patulin in the apple juice. The method has the beneficial effects that the patulin in the apple juice can be effectively removed by using the water insoluble corn flour, and the method is low in cost, simple in operation, green and safe, has a good adsorption effect and facilitates industrialized production.

The present invention provides an ultrafiltration / microfiltration membrane comprising a matrix filter membrane and a sulfhydryl-modified polydopamine membrane layer deposited on the surface of the matrix filter membrane, wherein the surface of the sulfhydryl-modified polydopamine membrane layer is chemically bonded with free sulfhydryl groups. The sulfhydryl-modified polydopamine membrane layer isdeposited on the surface of the matrix filter membrane, the adsorption and removal of patulin in liquid containing patulin such as fruit juice can be effectively realized, the adsorption capacity canreach 30[mu]g / cm<2>, which solves the problems of residual adsorbents, low removal efficiency and difficult industrial application in the traditional separation process of patulin.

The invention discloses a protein for degrading patulin and an encoding gene and application of the protein. The protein is (a) or (b). The protein (a) is a protein composed of an amino acid sequence shown in the sequence 1 in a sequence table. The protein (b) is a protein which has the patulin degrading function, is derived from the sequence 1 and is prepared in the mode that the amino acid sequence shown in the sequence 1 is subjected to substitution and / or deletion and / or addition of one or more amino acid residues. Under the condition that oxidized coenzyme exists, patulin can be degraded in vitro by using the protein, and the effect is very good. The protein has important application value in the aspect of eliminating patulin.

The invention relates to a preparation method of an electrochemical aptamer ratio sensor for patulin detection. The preparation method comprises the following steps: preparing trimetal nanoflowers bya hydrothermal synthesis method to be used as a substrate material; modifying a substrate material and a nucleic acid aptamer on the surface of the gold electrode by covalent binding; and preparing anucleic acid single-stranded probe by adopting an enzyme circulation cutting method to prepare a nucleic acid aptamer / trimetal composite nanoflower / aminated gold electrode. The electrode system is adopted, the electrochemical aptamer sensor for detecting the content of patulin is obtained by taking the nucleic acid aptamer / trimetal composite nanoflower / amination gold electrode as the working electrode, the platinum wire electrode as the counter electrode and saturated silver chloride as the reference electrode and measuring the concentration of patulin through the change of the signal ratio ofthe electroactive substance. Compared with a common single-signal electrochemical sensor, the electrochemical aptamer ratio sensor has the advantages of being high in response speed, low in background noise, high in sensitivity, good in repeatability and high in accuracy.