Method for detecting patulin and chloramphenicol in hawthorn products

A technology of patulin and a detection method, applied in the field of food or drug detection, can solve the problems of drug resistance, residue and harm of pathogenic bacteria, and achieve the effects of good stability, high accuracy and high detection efficiency

Active Publication Date: 2018-04-20
蒙小翠
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The chemical properties of chloramphenicol are relatively stable, and it is easy to remain in animals, causing direct harm. What is more serious is that a certain concentration of drug residues will induce drug resistance of pathogenic bacteria

Method used

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  • Method for detecting patulin and chloramphenicol in hawthorn products
  • Method for detecting patulin and chloramphenicol in hawthorn products
  • Method for detecting patulin and chloramphenicol in hawthorn products

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Experimental program
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Effect test

Embodiment 1

[0026] The detection method of patulin and chloramphenicol in hawthorn products, its detection method comprises the following steps:

[0027] (1) Preparation of sample solution and blank matrix solution: Take 5g of hawthorn products and blank matrix samples, crush them, and weigh them accurately; add 30ml of methanol, ultrasonicate for 40 minutes, shake well, and filter; respectively draw 1ml of the filtrate accurately, Add methanol to dilute 10 times, shake well, pass 0.2 mu m filter membrane, the sample solution to be tested and the blank matrix solution can be obtained;

[0028] (2) Preparation of mixed reference substance solution: Accurately weigh patulin and chloramphenicol reference substances respectively, add blank matrix solution to dilute to prepare a mixed solution with the mass concentration of patulin and chloramphenicol both at 1 μg / ml, and obtain mixed Reference substance stock solution: Accurately measure the above-mentioned mixed reference substance stock s...

Embodiment 2

[0035] The detection method of patulin and chloramphenicol in hawthorn products, its detection method comprises the following steps:

[0036] (1) Preparation of sample solution and blank matrix solution: take 5g of hawthorn products and blank matrix samples, crush them, and weigh them accurately; add 30ml of methanol, ultrasonicate for 30 minutes, shake well, and filter; respectively draw 1ml of the filtrate accurately, Add methanol to dilute 10 times, shake well, pass 0.2 mu m filter membrane, the sample solution to be tested and the blank matrix solution can be obtained;

[0037] (2) Preparation of mixed reference substance solution: Accurately weigh patulin and chloramphenicol reference substances respectively, add blank matrix solution to dilute to prepare a mixed solution with the mass concentration of patulin and chloramphenicol both at 1 μg / ml, and obtain mixed Reference substance stock solution: Accurately measure the above-mentioned mixed reference substance stock s...

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Abstract

The invention discloses a method for detecting patulin and chloramphenicol in hawthorn products. Testing is carried out by using a liquid chromatography-mass spectrometry method, the liquid chromatography conditions are as follows: the spectrum column Agilent Eclipse Plus C18 is 1.8mu m, 2.1mm*100mm, the column temperature is 33-38 DEG C, and the flowing speed is 0.2-0.5ml / min; gradient elution iscarried out with 9mmol / L ammonium acetate as a flow phase A and methanol as a flow phase B, and the sampling feeding amount is 1-2mu l; the mass spectrum conditions are as follows: an electrospray ionization source is adopted, detection is carried out in an MRM (Multiple Reaction Monitoring) mode, the temperature of a drying gas is 210 DEG C, the pressure of an atomizer is 20Psi, the flowing speed of the drying gas is 11-12L / minute, the temperature of a sheath flow gas is 350 DEG C, the flowing speed of the sheath flow gas is 10L / minute, and the voltage of a nozzle is 1000V. The method disclosed by the invention has the advantages of being simple, convenient and rapid, high in accuracy and good in stability, and is applicable to quality control on hawthorn products in laboratories, and on-batch detection with high detection efficiency can be achieved by using the method.

Description

technical field [0001] The invention relates to the field of food or medicine detection, in particular to a detection method for patulin and chloramphenicol in hawthorn products. Background technique [0002] Patulin, also known as patulin, patulin, and coral penicillin, is a secondary metabolite produced by fungi such as Aspergillus and Penicillium. Patulin has broad-spectrum antibiotic characteristics. Toxicological tests have shown that patulin has toxicological effects such as affecting fertility, carcinogenesis and immunity, and is also a neurotoxin. Patulin is teratogenic and very harmful to the human body, causing damage to the respiratory and urinary systems, nerve paralysis, pulmonary edema, and renal failure. [0003] Chloramphenicol, also known as chloramphenicol, is a broad-spectrum antibiotic produced by Streptomyces chloride and has the effect of inhibiting bacterial growth. Chloramphenicol has inhibitory effects on Gram-negative bacteria and Gram-positive bac...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 蒙小翠邓喜然
Owner 蒙小翠
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