Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Protein for degrading patulin and encoding gene and application of protein

A patulin and coding technology, applied in the field of microorganisms, can solve the problems such as the effect is not very ideal, and achieve the effect of good effect

Active Publication Date: 2016-12-21
INST OF BOTANY CHINESE ACAD OF SCI
View PDF3 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, patulin is mainly removed by physical means such as adsorption and microwave treatment, but the effect is not very satisfactory, and it is urgent to develop a new method for degrading patulin

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Protein for degrading patulin and encoding gene and application of protein
  • Protein for degrading patulin and encoding gene and application of protein
  • Protein for degrading patulin and encoding gene and application of protein

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Cloning of embodiment 1, Cgscd gene and prokaryotic expression of Cgscd protein

[0047] 1. Cloning of Cgscd gene

[0048] According to the GI number of Cgscd protein (gi|190348612, Short-chain dehydrogenase), look up the corresponding Cgscd gene sequence in NCBI, design primers at both ends of the gene and add BamHI and XhoI restriction sites to the primers, and name them respectively are pET-30a-F(BamHI) and pET-30a-R(XhoI).

[0049] pET-30a-F(BamHI): 5'- GGATCC ATGGAACAAACGTACTTTTATTTCAGGCG-3';

[0050] pET-30a-R(XhoI): 5'- CTCGAG TTACCATGGAAGTTCGGTTCCATCG-3'.

[0051]Candida guilliermondii (Candida guilliermondii) CGMCC 2.63 (recorded in "Yuanyuan Zong, Jia Liu, Boqiang Li, et al. Effects of yeast antagonists in combination with hot water treatment on postharvest diseases of tomato fruit. Biological Control 54 (2010) 316 -321 "article, the public can obtain from the applicant, can only be used for repeating the genomic DNA of the experiment of the present inv...

Embodiment 2

[0076] Example 2, Cgscd protein degradation research on patulin

[0077] After the concentration of the purified Cgscd protein in Example 1 was measured by the Coomassie brilliant blue method, the purified protein was taken to explore the effects of different coenzymes, pH and co-cultivation temperature on the catalytic degradation of patulin in vitro by the Cgscd protein.

[0078] Patulin: Canada Toronto Research Chemicals (TRC) company product, its catalog number is P206500.

[0079] 1. Coenzyme

[0080] It is divided into 4 groups: (1) reduced coenzyme Ⅰ (NADH); (2) oxidized coenzyme Ⅰ (NADH) + ); (3) reduced coenzyme Ⅱ (NADPH); (4) oxidized coenzyme Ⅱ (NADP + ).

[0081]The total volume of the reaction system is 300 μL, which contains 245 μL of 50 mM MES buffer solution (pH6) (preparation method: MES is dissolved in an appropriate amount of water, and the pH is adjusted to 6 with 2M NaOH, and then the water is made to volume so that the final concentration of MES in the...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
wavelengthaaaaaaaaaa
Login to View More

Abstract

The invention discloses a protein for degrading patulin and an encoding gene and application of the protein. The protein is (a) or (b). The protein (a) is a protein composed of an amino acid sequence shown in the sequence 1 in a sequence table. The protein (b) is a protein which has the patulin degrading function, is derived from the sequence 1 and is prepared in the mode that the amino acid sequence shown in the sequence 1 is subjected to substitution and / or deletion and / or addition of one or more amino acid residues. Under the condition that oxidized coenzyme exists, patulin can be degraded in vitro by using the protein, and the effect is very good. The protein has important application value in the aspect of eliminating patulin.

Description

technical field [0001] The invention belongs to the field of microorganisms, and relates to a protein for degrading patulin, its coding gene and application. Background technique [0002] Patulin is a secondary metabolite of some Penicillium and Aspergillus fungi. It has extensive and strong toxic effects on humans and animals. Acute toxicity manifests as convulsions, convulsions, subcutaneous tissue edema, pulmonary and intestinal congestion, enteritis, anuria, and death; chronic toxicity can lead to gastric ulcers, immunosuppression, teratogenicity, and potential carcinogenicity, etc., and may also affect men of fertility. Patulin is soluble in water, ethanol, acetone, ethyl acetate and chloroform, slightly soluble in ether and benzene, insoluble in petroleum ether, and chemically stable under acidic conditions. [0003] Among the fungi capable of producing patulin, some fungi are plant pathogens, and the most important one is Penicillium expansum. This pathogen is one...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/40C12N15/31A62D3/02A62D101/28
CPCA62D3/02A62D2101/28C07K14/40
Inventor 田世平李博强张占全
Owner INST OF BOTANY CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com