Various mycotoxin quantifying detection protein chip and kit thereof

A mycotoxin and protein chip technology, which is applied in biological testing, measuring devices, material inspection products, etc., can solve the problems of single function and slow efficiency, and achieve the effect of improving efficiency

Inactive Publication Date: 2010-06-16
上海裕隆生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention provides a protein chip for the quantitative detection of multiple mycotoxins and its kit, which can detect multiple mycotoxins in a one-time integrated manner, solve the problem of single function and slow efficiency of the detection reagents in the current food industry, and meet the multiple requirements of the current food industry. The development needs of modernization and industrialization

Method used

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  • Various mycotoxin quantifying detection protein chip and kit thereof
  • Various mycotoxin quantifying detection protein chip and kit thereof
  • Various mycotoxin quantifying detection protein chip and kit thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Preparation of a protein chip for quantitative detection of multiple mycotoxins

[0030] The main steps are: (1) Prepare the matrix nitrocellulose membrane; (2) Design the chip, determine the arrangement of the lattice and the spotting position; (3) Draw the antibody from the 384-well plate and spray it on the nitrocellulose (4) assemble a nitrocellulose membrane, and carry out sealing, drying, packaging, and preservation.

[0031] The above method for preparing a protein chip for quantitative detection of various mycotoxins specifically includes the following steps:

[0032] 1. Determination of mycotoxin detection antigen antibody

[0033] Determination of detection index antigen:

[0034] Select 7 kinds of mycotoxins that are more harmful and more widespread as antigens, extract and purify from raw materials. Take the extraction of aflatoxin as an example: the raw material containing aflatoxin will be extracted with a certain proportion of 40% methanol extract. Af...

Embodiment 2

[0042] Kit preparation

[0043] A kit for protein chips for quantitative detection of multiple mycotoxins, consisting of protein chips for quantitative detection of multiple mycotoxins, standard products, quality control products, sample diluents, enzyme-labeled working solutions, concentrated washing solutions, and detection solutions; each part It is bottled and fixed in the box with a paper template (as shown in the figure

[0044] 2), there is no limit to the placement method, and it is used in conjunction with the detection. Here's how the parts are prepared:

[0045] 1. The protein chip for the quantitative detection of multiple mycotoxins is a 6×8-well plate, with nitrocellulose membrane as the base, and the assembly method is shown in ZL032298900. After the completion of the appearance, see figure 1 .

[0046] 2. The standard product provides 6 bottles, each bottle is 0.25ml.

[0047]Prepare with phosphate buffer (pH 7.4) containing 3% BSA and 0.05% preservative (...

Embodiment 3

[0065] Application and detection of protein chips and kits

[0066] The application of the protein chip for the quantitative detection of various mycotoxins according to the present invention mainly adopts the antibody-antigen-enzyme-labeled secondary antibody method to detect the mycotoxins in the sample. The method is: solid samples such as grains, soybeans, etc. Crush, take 1g and put it into 1ml sample diluent, mix well, let the sample fully react with the sample diluent for half an hour, then absorb part and add it to the surface of the chip; for liquid samples such as juice, grain oil, etc., directly place 1ml in the 1ml of sample diluent, mix well, let the sample fully react with the sample diluent for half an hour, then absorb the part and add it to the surface of the chip. The mycotoxin in the sample, as an antigen-like component, binds to the corresponding specific antibody immobilized on the chip. After drying, add the enzyme-labeled secondary antibody to react, was...

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Abstract

The invention discloses a various mycotoxin quantifying detection protein chip and a kit thereof. The chip comprises a substrate and a point coating layer of arrayed mycotoxin antibodies which contains seven mycotoxin antibodies formed by uniformly distributing anti-aflatoxin B1, anti-ochratoxin A, anti-fumonisins, anti-vomitoxin, anti-zearalenone, anti-T-2 toxin and anti-patulin on the substrate. A detecting result with various indications is acquired by reacting once by using the protein chip of the invention. The method can be widely applied to the safety sanitation detection for products such as popular food, grain and oil food, feeding stuff, juice beverage and the like and can meet the requirement of the modern food industry to rapidly, simply and efficiently detect the safety of food.

Description

technical field [0001] The invention relates to a protein chip mycotoxin detection product, in particular to a protein chip for the quantitative detection of various mycotoxins and a kit thereof. Background technique [0002] As staple foods for daily consumption, the problem of mycotoxins in grains, oils, foods, and juice drinks has become increasingly prominent and has attracted widespread attention from industry insiders. The pollution of food and feed by mold is very common. In addition to causing deterioration, it will also produce mycotoxins with "three-cause" (teratogenic, carcinogenic, and mutagenic) effects, which will not only reduce the nutritional value of food and feed, but also reduce the nutritional value of food and feed. Easily lead to human and livestock poisoning. According to statistics, 25% of the world's grain and oil crops are contaminated with mycotoxins every year, and the Food and Agriculture Organization of the United Nations estimates that the re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/558
Inventor 穆海东汪宁梅穆宇豪刘纲奚鹰
Owner 上海裕隆生物科技有限公司
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